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Some retrospective studies reported that psoriasis (PsO) and psoriatic arthritis (PsA) may have been associated with an elevated risk of skin cancer. The causal associations among them remain unclear. To evaluate the causal association of among both PsO and PsA, and skin cancer. We performed large-scale two-sample and Multivariate Mendelian randomization analyses to examine whether there is a causal relationship between PsO and PsA, and skin cancer, encompassing basal cell carcinoma (BCC), cutaneous squamous cell carcinoma (cSCC), and cutaneous melanoma (CM). Genetically predicted PsO, per log-odds ratio increase, showed no significant association with the risk of BCC, cSCC, and CM. The odds ratios (with corresponding 95% confidence intervals) for BCC, cSCC, and CM were 1.00 (0.99,1.01) (P = 0.990), 0.94(0.89, 1.00) (P = 0.065), and 0.99 (0.98, 1.01) (P = 0.239), respectively. PsA showed a significant association with a decreased risk of BCC, with odds ratios (with corresponding 95% confidence intervals) of 1.00 (1.00, 1.00) (P = 0.214) and 1.00 (1.00, 1.00) (P = 0.477), respectively. Univariate analysis of the FinnGen database demonstrated PsA did exhibit a significant association with the decrease risk of BCC, with an odds ratio of 0.94(0.90,0.99) (P = 0.016). However, this association disappeared after other risk factors were adjusted. Our findings suggest no causal association between PsO and PsA and the genetic risk of skin cancer. Further observational studies are required to elucidate the relationship among PsO, PsA, and skin cancer.

Acute Myeloid Leukemia (AML) is a heterogeneous disease with limited therapeutic options and poor prognosis for patients. Therefore, the aim of this study was to evaluate the potential value of TRIM6 as a prognostic marker in AML. In this study, the low-expressed gene TRIM6 was obtained by analysis of The Cancer Genome Atlas (TCGA-AML) and Genotype-Tissue Expression (GTEx) databases.The prognostic impact of TRIM6 was analyzed using Kaplan-Meier curves, univariate COX, multivariate COX, and columnar plot models. The signaling pathways associated with TRIM6 were obtained using Gene Set Variation Analysis (GSVA) and Gene Set Enrichment Analysis (GSEA) methods. Infiltrating immune cells associated with TRIM6 expression were analyzed using the \"CIBERSORT\" method. Mutations in AML patients were characterized using relevant Single Nucleotide Polymorphism (SNP) data. The effect of TRIM6 expression on AML cell progression was further analyzed by qRT-PCR, CCK-8 assay, flow cytometry and Western blot. By analyzing TCGA-AML and GTEx data, TRIM6 was found to be under-expressed in AML patients, and Kaplan-Meier curves, one-way and multifactorial Cox regression models suggested that under-expression of TRIM6 had a poor prognosis. In addition, monocyte, M2 macrophage and memory B cell infiltration levels were found to be higher in the TRIM6 low-expression group than in the TRIM6 high-expression group based on the \"CIBERSORT\" method. Further mutational characterization showed that TRIM6 expression was positively correlated with NPM1 mutations and negatively correlated with mutations in RUNX1, TP53 and ASXL1. Enrichment analysis revealed that TRIM6 expression was associated with the PI3K/AKT signaling pathway and immune response. In addition, in vivo and in vitro experiments further demonstrated that TRIM6 expression could inhibit AML progression via PI3K/AKT. TRIM6 is expressed at low levels in AML, correlates with immune infiltration, and may affect AML progression through the PI3K/AKT signaling pathway.

In the process of double-shield TBM excavation, it is difficult to directly observe and test the characteristics of the surrounding rock. In this paper, the screening test of the different type tailings in the wet state was carried out to obtain the gradation curve and curve evaluation index. Combining with the excavation parameters and the surrounding rock characteristics of the tailings, a comprehensive analysis was carried out to establish the evaluation system among with the tailings gradation characteristics, lithology characteristics and excavation parameters. The results showed that: Sparsely fissured rock: the tailings are extensional fractures, the tailings gradation curve is inverse “ S ” type and gentle, and the evaluation index value: (1.50 > lg( Cu ) > 1.35), (1.90 >  C c  > 1.10). Broken surrounding rock: the curve is “ L ” type and steep, the content of coarse particles is much more than that of fine particles and (1.10 > lg( Cu ) > 1.00), (2.60 >  C c  > 2.40). Fractured rock: the curve is “ Step ” type, the tailings particles lack the middle particle size, the minerals are mostly weathered, (2.15 > lg( Cu  )> 1.95), (0.09 >  C c  > 0.07). The research results have good applicability to the surrounding rock stability evaluation of the example tunnel, which verifies the feasibility of the method.

The current green building assessment system suffers from issues such as insufficient coverage of smart indicators, significant biases in subjective weighting, and weak dynamic adaptability, which restrict the scientific promotion of green buildings. This study focuses on the gaps in the quantitative assessment of smart technologies in China’s green building evaluation standards (such as the current Green Building Evaluation Standard). While domestic standards are relatively well-established in traditional dimensions like energy conservation and environmental protection, there are fragmentation issues in the assessment of smart technologies such as the Internet of Things (IoT) and BIM. Moreover, the coverage of smart indicators in non-civilian building fields is significantly lower than that of international systems such as LEED and BREEAM. This study determined the basic framework of the evaluation indicator system through the Delphi method. Drawing on international experience and contextualized within China’s (GB/T 50378-2019) standards, it systematically integrated secondary indicators including “smart security,” “smart energy,” “smart design,” and “smart services,” and constructed dual-drive evaluation dimensions of “greenization + smartization.” This elevated the proportion of the smartization dimension to 35%, filling the gap in domestic standards regarding the quantitative assessment of smart technologies. In terms of research methods, combined weighting using the Analytic Hierarchy Process (AHP) and entropy weight method was adopted to balance subjective and objective weights and reduce biases (the resource conservation dimension accounted for 39.14% of the combined weights, the highest proportion). By integrating the grey clustering model with the whitening weight function to handle fuzzy information, evaluations were categorized into four grey levels (D/C/B/A), enhancing the dynamic adaptability of the system. Case verification showed that Project A achieved a comprehensive evaluation score of 5.223, with a grade of B. Among its indicators, smart-related ones such as “smart energy” (37.17%) and “smart design” (37.93%) scored significantly higher than traditional indicators, verifying that the system successfully captured the project’s high performance in smart indicators. The research results indicate that the efficient utilization of resources is the core goal of green buildings. Especially under pressures of energy shortages and carbon emissions, energy conservation and resource recycling have become key priorities. The evaluation system constructed in this study can provide theoretical guidance and technical support for the promotion, industrial upgrading, and sustainable development of green buildings (including non-civilian buildings) under the dual-carbon goals. Its characteristic of “dynamic monitoring + smart integration” forms differentiated complementarity with international standards, making it more aligned with the needs of China’s intelligent transformation of buildings.

Urbanization is a human‐induced process of rapid habitat change that can affect the reproductive success of animals. The occurrence of extra‐pair paternity (EPP) may be sensitive to changes in urban ecological conditions. In this study, the Chinese blackbird Turdus mandarinus, a common breeding bird in southern Chinese cities, was selected as the focus species to explore the EPP patterns of monogamous birds in developing cities and the relationship between pairs. Over four breeding seasons, 316 Chinese blackbird nestlings and 20 adults in both urban and rural areas were monitored and genotyped for 11 microsatellite loci to determine progeny affinity. We confirmed that both the brood size and hatching rate of EPP nests were significantly larger than those of within‐pair paternity (WPP) nests. The body mass, beak length, head length, and body length of 12‐day age extra‐pair offspring (EPO) were significantly greater than those of within‐pair offspring (WPO), suggesting important benefits of EPP. However, in accordance with the analyses of nest ecological variables, we concluded that EPP occurs most often in areas with low urbanization levels, indicating some constraints for this reproductive strategy in cities. Our findings provide new insights about how this growing human‐induced landscape change affects mating behaviour in birds, an important aspect in avian ecology and evolution.

Background Complicated acetabular fractures comprise the most challenging field for orthopedists. The purpose of this study was to develop three-dimensional printed patient-specific (3DPPS) Ti-6Al-4 V plates to treat complicated acetabular fractures involving quadrilateral plate (QLP) disruption and to evaluate their efficacy. Methods Fifty patients with acetabular fractures involving QLP disruption were selected between January 2016 and June 2017. Patients were divided into a control group (Group A, 35 patients) and an experimental group (Group B, 15 patients), and were treated by the conventional method of shaping reconstruction plates or with 3DPPS Ti-6AL-4 V plates, respectively. The efficacy of Ti-6AL-4 V plates was evaluated by blood loss, operative time, reduction quality, postoperative residual displacement, and complications. Results The operative time and blood loss in Group B were reduced compared to Group A, and the difference was statistically significant ( P  < 0.05). There was no significant difference in reduction quality between the two groups ( P  > 0.05). Reduction quality in Group B was anatomic in 10 (66.7%), satisfactory in four (26.7%), and poor in one (6.7%). In Group A, they were anatomic in 18 (51.4%), satisfactory in 13 (37.1%), and poor in four (11.4%). Residual displacement in Group B was less than that in Group A, and the difference was statistically significant ( P  < 0.05). In Group B, one case exhibited loosening of the pubic screw postoperatively. In Group A, there was one case of wound infection, one of deep vein thrombosis (DVT) in the ipsilateral lower limb, one case of traumatic arthritis and two obturator nerve injuries. Conclusions The 3DPPS Ti-6AL-4 V plate is a feasible, accurate and effective implant for acetabular fracture treatment.

Melanoma is among the most malignant immunologic tumor types and is associated with high mortality. However, a considerable number of melanoma patients cannot benefit from immunotherapy owing to individual differences. This study attempts to build a novel prediction model of melanoma that fully considers individual differences in the tumor microenvironment. An immune-related risk score (IRRS) was constructed based on cutaneous melanoma data from The Cancer Genome Atlas (TCGA). Single-sample gene set enrichment analysis (ssGSEA) was used to calculate immune enrichment scores of 28 immune cell signatures. We performed pairwise comparisons to obtain scores for cell pairs based on the difference in the abundance of immune cells within each sample. The resulting cell pair scores, in the form of a matrix of relative values of immune cells, formed the core of the IRRS. The area under the curve (AUC) for the IRRS was over 0.700, and when the IRRS was combined with clinical information, the AUC reached 0.785, 0.817, and 0.801 for the 1-, 3-, and 5-year survival, respectively. Differentially expressed genes between the two groups were enriched in staphylococcal infection and estrogen metabolism pathway. The low IRRS group showed a better immunotherapeutic response and exhibited more neoantigens, richer T-cell receptor and B-cell receptor diversity, and higher tumor mutation burden. The IRRS enables a good prediction of prognosis and immunotherapy effect, based on the difference in the relative abundance of different types of infiltrating immune cells, and could provide support for further research in melanoma.

Inhibitors of programmed cell death protein 1 and its associated ligand (PD-L1) are widely used in cancer treatment. However, medical costs and benefits of PD-1/PD-L1 inhibitors need attention owing to differences in response rates among individuals. This study explored global trends in the health economics field of PD-1/PD-L1 inhibitors to enhance their worldwide development. Bibliometric analysis of all documents currently indexed in Web of Science Core Collection from inception to 2022 was performed. Publication year, authors, countries, institutes, and journals were analyzed by Bibliometrix package (version 3.2.1) in R (version 4.1.3). CiteSpace (version 6.1.R6) and VOSviewer (version 1.6.18) were used to analyze burst words, co-authorship of institutes, co-cited journals, and co-cited references, while figures were mainly drawn by Ggplot2 package (version 3.3.5) in R (version 4.1.3) and SCImago Graphica Beta (version 1.0.23). A total of 2020 documents related to the health economics of PD-1/PD-L1 inhibitors were identified, and 1,204 documents met the selection criteria for inclusion in the study. A rapid increase in the number of publications since 2019 was observed, but this increase stopped in 2022, revealing research saturation in the field. Value in Health (166 publications, 13.79% of total documents) had the most publications, while New England Journal of Medicine (2,890 co-citations) was the most co-cited journal. The United States was the leading contributor in this field with 506 publications and the top two productive institutes globally. The main hot topics included the cost-effectiveness of treatment with PD-1 and/or PD-L1 inhibitors, and the comparison between the cost-effectiveness of PD-/PD-L1 inhibitors and other drugs. There were substantial differences between developed and developing countries in the health economics field of PD-1 and/or PD-L1 inhibitors. The cost-effectiveness analysis of combined treatment with PD-1/PD-L1 inhibitors and other drugs warrants further attention. Findings from this study may provide governments and pharmaceutical companies with a strong reference for future research.

Background Pelvic fractures are rare in toddlers but are often associated with other injuries that make treatment difficult. Conservative treatment has been used with moderate success, but it is unclear if surgical correction could confer additional benefits and improve patient outcomes. The purpose of this study was to report authors’ experience using the lateral-rectus approach (LRA) for surgical correction of unstable pelvic fractures in two toddlers. Methods We retrospectively analyzed the cases of two toddlers with unstable pelvic fractures who underwent surgery through the LRA between April 2016 and October 2018. Patients’ characteristics, fracture type, mechanism of injury, Injury Severity Score (ISS), operative time, intra-operative blood loss, and post-operative complications were assessed. Pelvic asymmetry, degree of deformity, Cole scoring criteria and modified Barthel Index (MBI) were used to evaluate radiographic and functional outcomes. Results Successful surgical treatment was performed using the LRA, external fixation, and sacroiliac screw fixation. Surgery duration was 180 min on average, with an average intra-operative bleeding of 250 ml. There were no iatrogenic nerve injuries or infections. Pelvic asymmetry a week after surgery was 0.5 cm on average and dropped to 0.3 cm on average at the end of the follow-up period. The deformity index of the pelvis dropped from an average of 0.035 a week after surgery to 0.02 at the end of the follow-up period. The mean MBI was 100 in the last follow-up, and Cole scoring criteria categorized both patients as being in excellent condition. All patients achieved radiological bone union without discrepancy in length of the lower limbs. Neither patient had loss of reduction nor evidence of low back pain during the mean follow-up period of 22 months. Conclusions Pelvic fracture in toddlers is rare, and surgical treatment requires careful consideration. The lateral-rectus approach was proven as a viable alternative for managing unstable pelvic fractures in toddlers, with minimal blood loss and risk of nerve injury. Furthermore, anterior external fixation and posterior sacroiliac screw fixation would be adequate for this population, with excellent final outcome.

Objective This study aimed to investigate the regulatory effects of long non-coding RNA-ANRIL on CDKN2A in the cell cycle of Kasumi-1 cells and elucidate the underlying molecular mechanisms. Methods ANRIL and CDKN2A expression levels were quantified using RT-qPCR in peripheral blood samples from acute myeloid leukemia (AML) patients. CDKN2A knockdown efficiency was validated via RT-qPCR, and cell cycle distribution was analyzed using flow cytometry. Cell proliferation assays were conducted with CCK-8 following palbociclib treatment and CDKN2A downregulation. RNA immunoprecipitation (RIP) identified potential ANRIL-associated targets, while western blotting assessed the expression levels of GSK3β/β-catenin/cyclin D1 signaling components and related proteins. Results ANRIL and CDKN2A were markedly overexpressed in AML patient samples. Knockdown of ANRIL and CDKN2A led to G1 phase arrest accompanied by reduced CDK2/4/6 and cyclin D1 protein levels, while ANRIL upregulation induced the opposite effect. Palbociclib treatment for 24 h and 48 h elevated the G1 phase cell population and suppressed CDK2/4/6 and cyclin D1 protein expression, demonstrating its ability to counteract ANRIL-driven cell cycle progression. Downregulation of ANRIL and CDKN2A also suppressed the GSK3β/β-catenin signaling pathway, reducing cyclin D1 expression, whereas ANRIL upregulation reactivated this axis. Co-transfection experiments showed that simultaneous cyclin D1 suppression and ANRIL overexpression attenuated ANRIL’s stimulatory effects on cell cycle progression. RIP analysis confirmed a physical interaction between ANRIL and CDKN2A. Furthermore, CDKN2A downregulation inhibited cell proliferation and reversed GSK3β/β-catenin/cyclin D1 pathway activation mediated by ANRIL upregulation. Conclusion ANRIL facilitates Kasumi-1 cell survival by modulating CDKN2A to activate the GSK3β/β-catenin/cyclin D1 signaling pathway.