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Continuous canopy status monitoring is an essential factor to support and precisely apply orchard management actions such as pruning, pesticide and foliar treatment applications, or fertirrigation, among others. For that, this work proposes the use of multispectral vegetation indices to estimate geometric and structural orchard parameters from remote sensing images (high temporal and spatial resolution) as an alternative to more time-consuming processing techniques, such as LiDAR surveys or UAV photogrammetry. A super-intensive almond (Prunus dulcis) orchard was scanned using a mobile terrestrial laser (LiDAR) in two different vegetative stages (after spring pruning and before harvesting). From the LiDAR point cloud, canopy orchard parameters, including maximum height and width, cross-sectional area and porosity, were summarized every 0.5 m along the rows and interpolated using block kriging to the pixel centroids of PlanetScope (3 × 3 m) and Sentinel-2 (10 × 10 m) image grids. To study the association between the LiDAR-derived parameters and 4 different vegetation indices. A canonical correlation analysis was carried out, showing the normalized difference vegetation index (NDVI) and the green normalized difference vegetation index (GNDVI) to have the best correlations. A cluster analysis was also performed. Results can be considered optimistic both for PlanetScope and Sentinel-2 images to delimit within-field management zones, being supported by significant differences in LiDAR-derived canopy parameters.

Eutrophication affects seagrasses negatively by increasing light attenuation through stimulation of biomass of fast-growing, bloom-forming algae and because high concentrations of ammonium in the water can be toxic to higher plants. We hypothesized nevertheless, that moderate amounts of nitrophilic macroalgae that coexists with seagrasses under eutrophic conditions, can alleviate the harmful effects of eutrophication on seagrasses by reducing ammonium concentrations in the seawater to non-toxic levels because such algae have a very large capacity to take up inorganic nutrients. We studied therefore how combinations of different ammonium concentrations (0, 25 and 50 μM) and different standing stocks of macroalgae (i.e. 0, 1 and 6 layers of Ulva sp.) affected survival, growth and net production of the seagrass Zostera noltei. In the absence of Ulva sp., increasing ammonium concentrations had a negative influence on the performance of Z. noltei. The presence of Ulva sp. without ammonium supply had a similar, but slightly smaller, negative effect on seagrass fitness due to light attenuation. When ammonium enrichment was combined with presence of Ulva sp., Ulva sp. ameliorated some of negative effects caused by high ammonium availability although Ulva sp. lowered the availability of light. Benthic microalgae, which increased in biomass during the experiment, seemed to play a similar role as Ulva sp.--they contributed to remove ammonium from the water, and thus, aided to keep the ammonium concentrations experienced by Z. noltei at relatively non-toxic levels. Our findings show that moderate amounts of drift macroalgae, eventually combined with increasing stocks of benthic microalgae, may aid seagrasses to alleviate toxic effects of ammonium under eutrophic conditions, which highlights the importance of high functional diversity for ecosystem resistance to anthropogenic disturbance.

Despite meticulous monitoring of Salmonella spp. throughout the food chain to ensure safer animal food products for consumers, the number of salmonellosis cases in humans continues to rise annually in Europe. Phage therapy emerges as a promising tool for controlling and eradicating Salmonella in primary production. This study aimed to fully characterize new phage therapy candidates isolated from animal sources. To achieve this, a phenotypic and genetic characterization of five phage isolates was conducted. The five phages demonstrated physical stability across a wide range of temperatures and pH levels, effectively lysing 12 different Salmonella serovars, including the most prevalent ones in the European Union in recent years, as well as multidrug-resistant strains isolated from the field. Additionally, four of the phages exhibited depolymerase production in the host range, with genomic analysis confirming that all five possessed sequences encoding for this activity, suggesting their potential as surface-disinfecting agents. Genetic analysis further revealed that the phages belong to distinct genera: Felixounavirus , Cornellvirus , Skatevirus , Agtevirus and Berlinvirus . Notably, none of the phages contained harmful sequences that could compromise their future application, such as virulence factors, antibiotic resistance genes or temperate markers. Overall, these five phages show promise as suitable candidates for phage therapy applications or phage-based Salmonella eradication strategies, where their integration in the existing biocontrol measures may enhance both food safety and public health.

The photoacclimation capacity of the seagrass Cymodocea nodosa was evaluated considering temporal (i.e. seasonal) and spatial (i.e. depth and within-leaf position) factors of variation. Changes along the leaf were measured in a population growing along a depth gradient (from intertidal to subtidal) in Cadiz Bay (Southern Spain) from 2004 to 2005. Photoacclimation was evaluated by photosynthesis ( P – E curves), pigment content and leaf morphology. Plants of Cymodocea nodosa showed large physiological and morphological plasticity (mean %CV = 35.8 ± 3.4) according to the three factors considered. Seasonal patterns appeared for photosynthesis, respiration, pigment content and morphology. Nevertheless, seasonal patterns were not consistent with depth or leaf portions. The resulting data set offered different information depending on the analysis conducted; when only one factor (season, depth or leaf portion) was considered, some tendencies observed in the 3-way full design were masked. Accordingly, considering spatio–temporal variability is crucial when describing photoacclimation and estimating productivity in seagrass meadows.

Knowledge of plant mechanical traits is important in understanding how plants resist abiotic and biotic forces and in explaining ecological strategies such as leaf lifespan. To date, these traits have not been systematically evaluated in seagrasses. We analysed mechanical (breaking force and tensile strength) and associated traits (thickness, width, length, fibre content, mass area, and lifespan) of leaves in 22 seagrass species (around one-third of all known seagrass species) to examine (1) the inter-specific variation of these traits in relation to growth form and bioregions, (2) the contribution of morphology to leaf breaking force, (3) how breaking force scales to leaf dimensions, and (4) how mechanical and structural traits correlate to leaf longevity. We also compared our seagrass dataset with terrestrial plant databases to examine similarities between them. Large variation in leaf breaking force was found among seagrass species but, on average, temperate species resisted higher forces than tropical species. Variation in leaf breaking force was largely explained by differences in leaf width rather than thickness, likely due to the benefits in leaf reconfiguration and light interception. Species of large dimensions (long leaves) typically had high leaf breaking force, plausibly to tolerate the drag forces they may experience, which are proportional to the leaf area. Leaves of long-lived species typically had high mass per leaf area and fibre content and they supported high breaking forces. Compared to terrestrial plants, seagrasses are short-lived species with moderately strong fibre-reinforced leaves, which probably evolved to withstand the hydrodynamic forces occurring in the sea, and in response to other environmental factors. Overall, our analysis provides new insights into the physical performance of seagrasses in the marine environment.

Regulatory T cells are a population of CD4+ T cells that play a critical role in peripheral tolerance and control of immune responses to pathogens. The purpose of this study was to measure the percentages of two different regulatory T cells subpopulations, identified by the presence or absence of CD31 (Recent thymic emigrants and peripherally induced naïve regulatory T cells), in term and preterm infant cord blood. We report the association of prenatal factors, intrauterine exposure to lipopolysaccharide and inflammation and the percentages of these regulatory T cell subpopulations in term and preterm infants. Cord blood samples were collected from both term and preterm infants and mononuclear cells isolated over a Ficoll-Hypaque cushion. Cells were then stained with fluorochrome-labeled antibodies to characterize regulatory T cell populations and analyzed with multi-color flow cytometry. Cord blood plasma C-reactive protein, and lipopolysaccharide were also measured. Placental pathology was also examined. We report a gestational age-dependent difference in the percentage of total regulatory T cells, in which preterm infants of lower gestational ages have an increased percentage of regulatory T cells. We report the presence of two populations of regulatory T cells (CD31+ and CD31-) in cord blood of term and preterm infants and their association with different maternal and fetal characteristics. Factors associated with differences in the percentage of CD31- Tregs included the use of prenatal antibiotics, steroids and magnesium sulfate. In addition, the percentage of CD31- Tregs was significantly higher in cord blood of preterm pregnancies associated with inflammation and prenatal lipopolysaccharide exposure. The peripheral Treg pool of preterm infants could be altered by prenatal exposure to inflammation and chorioamnionitis; however, the clinical implications of this finding are not yet understood.

Multi-junction solar cells made by assembling semiconductor materials with different bandgap energies have hold the record conversion efficiencies for many years and are currently approaching 50%. Theoretical efficiency limits make use of optimum designs with the right lattice constant-bandgap energy combination, which requires a 1.0–1.15 eV material lattice-matched to GaAs/Ge. Nevertheless, the lack of suitable semiconductor materials is hindering the achievement of the predicted efficiencies, since the only candidates were up to now complex quaternary and quinary alloys with inherent epitaxial growth problems that degrade carrier dynamics. Here we show how the use of strain-balanced GaAsSb/GaAsN superlattices might solve this problem. We demonstrate that the spatial separation of Sb and N atoms avoids the ubiquitous growth problems and improves crystal quality. Moreover, these new structures allow for additional control of the effective bandgap through the period thickness and provide a type-II band alignment with long carrier lifetimes. All this leads to a strong enhancement of the external quantum efficiency under photovoltaic conditions with respect to bulk layers of equivalent thickness. Our results show that GaAsSb/GaAsN superlattices with short periods are the ideal (pseudo)material to be integrated in new GaAs/Ge-based multi-junction solar cells that could approach the theoretical efficiency limit.

Rhizophytic green algae of the genus Caulerpa are potential competitors with seagrass-dominated habitats. At Cadiz Bay Natural Park, Caulerpa prolifera and the seagrass Cymodocea nodosa co-occur in overlapping patches, which may be considered as transition zones. In these zones, changes in microtopography are stepped because patches of C. prolifera normally occur at 5 to 10 cm above the adjacent C. nodosa patches. We hypothesized that hydrodynamics influenced by this microtopography may foster sedimentation on the C. nodosa side, which in turn would facilitate the spreading of C. prolifera. To test this hypothesis, an experiment simulating the flow between both species was conducted in a race-track flume tank, allowing us to study the influence of unidirectional free stream velocity (low velocity, LV = 0.065 m s−1, vs. high velocity, HV = 0.14 m s−1) and microtopography (flat bottom, FB, vs. stepped bottom, SB) on shear stress (τ), turbulent kinetic energy above the canopy (TKEabove) and volumetric flow rate through the canopy (Q c). Under our experimental conditions, comparison of τ-values with theoretical thresholds revealed sedimentation scenarios. However, at HV, the probability of sedimentation was higher in C. nodosa than in C. prolifera patches. Moreover, Q c was higher within C. nodosa canopies than within C. prolifera, ensuring a higher source of sediment with potential to be trapped within the canopy. Although we found no species-specific differences in TKEabove values, they increased with HV increasing the vertical mixing. Overall, conditions for sedimentation seemed more facilitated in C. nodosa than in C. prolifera in SB only under HV conditions.

Friedreich’s ataxia (FRDA) is a rare inherited recessive disorder affecting the central and peripheral nervous systems and other extraneural organs such as the heart and pancreas. This incapacitating condition usually manifests in childhood or adolescence, exhibits an irreversible progression that confines the patient to a wheelchair, and leads to early death. FRDA is caused by a reduced level of the nuclear-encoded mitochondrial protein frataxin due to an abnormal GAA triplet repeat expansion in the first intron of the human FXN gene. FXN is evolutionarily conserved, with orthologs in essentially all eukaryotes and some prokaryotes, leading to the development of experimental models of this disease in different organisms. These FRDA models have contributed substantially to our current knowledge of frataxin function and the pathogenesis of the disease, as well as to explorations of suitable treatments. Drosophila melanogaster, an organism that is easy to manipulate genetically, has also become important in FRDA research. This review describes the substantial contribution of Drosophila to FRDA research since the characterization of the fly frataxin ortholog more than 15 years ago. Fly models have provided a comprehensive characterization of the defects associated with frataxin deficiency and have revealed genetic modifiers of disease phenotypes. In addition, these models are now being used in the search for potential therapeutic compounds for the treatment of this severe and still incurable disease.

The effects of light and ammonium levels on net production, fluorescence parameters and non-structural carbohydrates of the seagrass Zostera noltii under different phosphate conditions were studied. A fully factorial design was used with light (low/high levels), ammonium supply and phosphate preculture conditions of the plants as the experimental variables. Both ammonium supply and low light caused negative and synergistic effects on net production, while ammonium toxicity was more severe at high light levels; in this case, it was independent of the non-structural carbohydrate (sucrose and starch) content. Preculturing of plant with added phosphate alleviated the ammonium toxicity, and also attenuated the negative production balance of plants grown at low light levels. The results indicated that phosphate preculture ameliorated the plant's short-term response against the assayed stressors (low light, high ammonium) significantly. An overall consumption of non-structural carbohydrates in response to environmental stressors was recorded throughout the experiment, indicating the importance of carbon and phosphorus reserves to cope with adverse conditions. ln addition, phosphate deficiency increased the vulnerability of plants, which could have negative ecological consequences for seagrass species thriving under phosphate deficiency conditions, or in developing seagrass transplantation programs.