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The 2021 volcanic eruption at La Palma, Canary Islands, was the island’s most voluminous historical eruption. Little is known about this volcano’s feeding system. During the eruption, seismicity was distributed in two clusters at ~10-14 km and ~33-39 km depth, separated by an aseismic zone. This gap coincides with the location of weak seismic swarms in 2017-2021 and where petrological data have implied pre-eruptive magma storage. Here we use seismological methods to understand the seismic response to magma transfer, with 8,488 hypocentral relocations resolving small-scale seismogenic structures, and 156 moment tensors identifying stress heterogeneities and principal axes flips. Results suggest a long-lasting preparatory stage with the progressive destabilisation of an intermediate, mushy reservoir, and a co-eruptive stage with seismicity controlled by the drainage and interplay of two localised reservoirs. Our study provides new insights into the plumbing system that will improve the monitoring of future eruptions in the island. In a new study, the authors use seismological methods to understand the eruption of La Palma 2021. Results suggest a preparatory phase of de-stabilisation of a mushy reservoir, and a co-eruptive phase with seismicity controlled by the drainage and interplay of two reservoirs.

Abstract Background: Maternal overweight, obesity, and gestational diabetes (GD) have been negatively associated with offspring development. Further knowledge regarding metabolic and nutritional alterations in these mother and their offspring are warranted. Methods: In an observational cohort study we included 331 pregnant women from Granada, Spain. The mothers were categorized into four groups according to BMI and their GD status; overweight (n:56), obese (n:64), GD (n:79), and healthy normal weight controls (n:132). We assessed maternal growth and nutritional biomarkers at 24 weeks (n = 269), 34 weeks (n = 310) and at delivery (n = 310) and the perinatal characteristics including cord blood biomarkers. Results: Obese and GD mothers had significantly lower weight gain during pregnancy and infant birth weight, waist circumference, and placental weight were higher in the obese group, including a significantly increased prevalence of macrosomia. Except for differences in markers of glucose metabolism (glucose, HbA1c, insulin and uric acid) we found at some measures that overweight and/or obese mothers had lower levels of transferrin saturation, hemoglobin, Vitamin B12 and folate and higher levels of C-reactive protein, erythrocyte sedimentation rate, ferritin, and cortisol. GD mothers had similar differences in hemoglobin and C-reactive protein but higher levels of folate. The latter was seen also in cord blood. Conclusions: We identified several metabolic alterations in overweight, obese and GD mothers compared to controls. Together with the observed differences in infant anthropometrics, these may be important biomarkers in future research regarding the programming of health and disease in children. Trial registration: The trial was registered at clinicaltrials.gov, identifier (NCT01634464). Keywords: Pregnancy, Maternal overweight, Maternal obesity, Gestational diabetes, Offspring, Fetal nutrition, Early programming, Vitamin B12, Folate, Iron status, Glucose metabolism

Anthocyanins are flavonoid pigments broadly distributed in plants with great potential to be used as food colorants due to their range of colors, innocuous nature, and positive impact on human health. However, these molecules are unstable and affected by pH changes, oxidation and high temperatures, making it very important to extract them using gentle non-thermal technologies. The use of emerging non-thermal techniques such as High Hydrostatic Pressure (HHP), Ultra High Pressure Homogenization (UHPH), Pulsed Electric Fields (PEFs), Ultrasound (US), irradiation, and Pulsed Light (PL) is currently increasing for many applications in food technology. This article reviews their application, features, advantages and drawbacks in the extraction of anthocyanins from grapes. It shows how extraction can be significantly increased with many of these techniques, while decreasing extraction times and maintaining antioxidant capacity.

Dietary (poly)phenols are extensively metabolized, limiting their anticancer activity. Exosomes (EXOs) are extracellular vesicles that could protect polyphenols from metabolism. Our objective was to compare the delivery to breast tissue and anticancer activity in breast cancer cell lines of free curcumin (CUR) and resveratrol (RSV) vs. their encapsulation in milk-derived EXOs (EXO-CUR and EXO-RSV). A kinetic breast tissue disposition was performed in rats. CUR and RSV were analyzed using UPLC-QTOF-MS and GC-MS, respectively. Antiproliferative activity was tested in MCF-7 and MDA-MB-231 breast cancer and MCF-10A non-tumorigenic cells. Cell cycle distribution, apoptosis, caspases activation, and endocytosis pathways were determined. CUR and RSV peaked in the mammary tissue (41 ± 15 and 300 ± 80 nM, respectively) 6 min after intravenous administration of EXO-CUR and EXO-RSV, but not with equivalent free polyphenol concentrations. Nanomolar EXO-CUR or EXO-RSV concentrations, but not free CUR or RSV, exerted a potent antiproliferative effect on cancer cells with no effect on normal cells. Significant (p < 0.05) cell cycle alteration and pro-apoptotic activity (via the mitochondrial pathway) were observed. EXO-CUR and EXO-RSV entered the cells primarily via clathrin-mediated endocytosis, avoiding ATP-binding cassette transporters (ABC). Milk EXOs protected CUR and RSV from metabolism and delivered both polyphenols to the mammary tissue at concentrations compatible with the fast and potent anticancer effects exerted in model cells. Milk EXOs enhanced the bioavailability and anticancer activity of CUR and RSV by acting as Trojan horses that escape from cancer cells’ ABC-mediated chemoresistance.

Vaccination rates among American Indians and Alaska Natives are higher than average. Since early in the pandemic, these communities have worked to protect their members, including by adapting community mitigation and vaccination strategies to their diverse cultures.

Entrepreneurial intention has been observed to be a necessary, but insufficient, condition for new business creation. Another variable apart from intention that requires consideration is how potential entrepreneurs perceive their own capacity or ability to exercise control over entrepreneurial behaviour. Drawing on the Rubicon model of action phases, this paper analyses the role of the perceived entrepreneurial competencies in the preactional phase of the entrepreneurial process. This corresponds to the moment when individuals have formed their entrepreneurial intention and must embark on how to implement it. A structural equations modelling analysis on a sample of undergraduate business students in their final year who manifest an intention to start up a new business after graduation served both to analyse the relationship between entrepreneurial competencies and gestation behaviour and determine the moderating role of competencies in the relationship between the intention and nascent activities. The findings reveal that entrepreneurial intention and competencies related to commitment, planning and organisation have a significant and direct influence on nascent entrepreneurial behaviour and that the whole set of entrepreneurial competency factors enhances the relationship between entrepreneurial intention and entrepreneurial gestation activities. This paper provides a comprehensive analysis of the role of entrepreneurial competencies in the transition from intention to early nascent business gestation and addresses a valuable and relatively unexplored line of research concerning the interaction effects of intention and perceived competencies on the performance of entrepreneurial gestation activities. The paper also delves into the practical implications for designers of entrepreneurship support and education programmes.

Faults in photovoltaic modules in operation can lead to power losses. By determining the module surface temperature, hot spots that can potentially cause this power loss can be detected. Temperature measurement by radiation allows a complete, reliable, and fast qualitative determination of hot spots on PV modules in outdoor operation. However, to obtain quantitative values, it is necessary to consider multiple factors: emissivity, reflected radiation, wind speed, intensity, shading, etc. Temperature quantitative measurement evaluation by contact is more studied, although by this technique it is impossible to examine the temperature of the entire module to detect hot spots because it is a point measurement and due to shading caused by the measurement probe on the surface. In this work, a method of temperature measurement by radiation is described, evaluating the uncertainty components, and a comparison is made with temperature measurement by contact on the module rear side points where module heating has been detected, also evaluating the uncertainty components. This comparison of both methods and uncertainty determination allows establishing a methodology in quantitative temperature measurement by radiation in photovoltaic modules in outdoor operation.

Production of industrially relevant compounds in microbial cell factories can employ either genomes or plasmids as an expression platform. Selection of plasmids as pathway carriers is advantageous for rapid demonstration but poses a challenge of stability. Yarrowia lipolytica has attracted great attention in the past decade for the biosynthesis of chemicals related to fatty acids at titers attractive to industry, and many genetic tools have been developed to explore its oleaginous potential. Our recent studies on the autonomously replicating sequences (ARSs) of nonconventional yeasts revealed that the ARSs from Y. lipolytica showcase a unique structure that includes a previously unannotated sequence (spacer) linking the origin of replication (ORI) and the centromeric (CEN) element and plays a critical role in modulating plasmid behavior. Maintaining a native 645-bp spacer yielded a 2.2-fold increase in gene expression and 1.7-fold higher plasmid stability compared to a more universally employed minimized ARS. Testing the modularity of the ARS sub-elements indicated that plasmid stability exhibits a pronounced cargo dependency. Instability caused both plasmid loss and intramolecular rearrangements. Altogether, our work clarifies the appropriate application of various ARSs for the scientific community and sheds light on a previously unexplored DNA element as a potential target for engineering Y. lipolytica.Key points• In Y. lipolytica, an ARS comprises an origin of replication, a spacer, and a centromere.• The unannotated spacer present in the wild-type ARS stabilizes episomal expression.• Plasmid stability and intramolecular rearrangements exhibit a clear cargo dependency.

This work is focused on deformation activity mapping and monitoring using Sentinel-1 (S-1) data and the DInSAR (Differential Interferometric Synthetic Aperture Radar) technique. The main goal is to present a procedure to periodically update and assess the geohazard activity (volcanic activity, landslides and ground-subsidence) of a given area by exploiting the wide area coverage and the high coherence and temporal sampling (revisit time up to six days) provided by the S-1 satellites. The main products of the procedure are two updatable maps: the deformation activity map and the active deformation areas map. These maps present two different levels of information aimed at different levels of geohazard risk management, from a very simplified level of information to the classical deformation map based on SAR interferometry. The methodology has been successfully applied to La Gomera, Tenerife and Gran Canaria Islands (Canary Island archipelago). The main obtained results are discussed.

The use of high-pressure technologies is a hot topic in food science because of the potential for a gentle process in which spoilage and pathogenic microorganisms can be eliminated; these technologies also have effects on the extraction, preservation, and modification of some constituents. Whole grapes or bunches can be processed by High Hydrostatic Pressure (HHP), which causes poration of the skin cell walls and rapid diffusion of the anthocyanins into the pulp and seeds in a short treatment time (2–10 min), improving maceration. Grape juice with colloidal skin particles of less than 500 µm processed by Ultra-High Pressure Homogenization (UHPH) is nano-fragmented with high anthocyanin release. Anthocyanins can be rapidly extracted from skins using HHP and cell fragments using UHPH, releasing them and facilitating their diffusion into the liquid quickly. HHP and UHPH techniques are gentle and protective of sensitive molecules such as phenols, terpenes, and vitamins. Both techniques are non-thermal technologies with mild temperatures and residence times. Moreover, UHPH produces an intense inactivation of oxidative enzymes (PPOs), thus preserving the antioxidant activity of grape juices. Both technologies can be applied to juices or concentrates; in addition, HHP can be applied to grapes or bunches. This review provides detailed information on the main features of these novel techniques, their current status in anthocyanin extraction, and their effects on stability and process sustainability.