Explore the vast range of titles available.

Angiogenesis and neurogenesis are functionally interconnected during brain development. However, the study of the vasculature has trailed other brain cell types because they are delicate and of low abundance. Here we describe a protocol extension to purify prenatal human brain endothelial and mural cells with FACS and utilize them in downstream applications, including transcriptomics, culture and organoid transplantation. This approach is simple, efficient and generates high yields from small amounts of tissue. When the experiment is completed within a 24 h postmortem interval, these healthy cells produce high-quality data in single-cell transcriptomics experiments. These vascular cells can be cultured, passaged and expanded for many in vitro assays, including Matrigel vascular tube formation, microfluidic chambers and metabolic measurements. Under these culture conditions, primary vascular cells maintain expression of cell-type markers for at least 3 weeks. Finally, we describe how to use primary vascular cells for transplantation into cortical organoids, which captures key features of neurovascular interactions in prenatal human brain development. In terms of timing, tissue processing and staining requires ~3 h, followed by an additional 3 h of FACS. The transplant procedure of primary, FACS-purified vascular cells into cortical organoids requires an additional 2 h. The time required for different transcriptomic and epigenomic protocols can vary based on the specific application, and we offer strategies to mitigate batch effects and optimize data quality. In sum, this vasculo-centric approach offers an integrated platform to interrogate neurovascular interactions and human brain vascular development. Key points This protocol extension describes the purification of prenatal human brain endothelial and mural cells with FACS and their utilization in downstream applications, including cell culture, organoid transplantation and single-cell transcriptomics. This simple, efficient protocol has relatively few steps compared with other methods and uses inexpensive reagents. Robust yields of healthy vascular and perivascular cells can be obtained in 6 h. A protocol extension describing the purification of prenatal human brain endothelial and mural cells with FACS and their utilization in downstream applications, including cell culture, organoid transplantation and single-cell transcriptomics.

Purpose This study aims to reflect on quality, sustainability and resilience as emerging organisational priorities within total quality management (TQM) and organisational excellence. Design/methodology/approach The paper uses a conceptual approach based on reflection and theoretical studies on the philosophical foundations of quality, excellence, resilience and sustainability as cornerstones for organisational excellence. Bearing in mind that sustainable excellence rests upon a combination of systemic and soft issues that define organisational ability for resilience and sustainability, there is a need to analyse and reflect on short business cases from world-leading companies and further reflect on the fundamental principles, which have helped such companies to survive, grow and sustain. This study includes such a business case – the LEGO case. In addition, a Japanese case has been included. Japanese training material on human motivation developed in the 1980s exemplifies how company managers were trained, at that time, to understand and practice human motivation, excellence principles and tools. Findings Organisational excellence constitutes an evolving concept as the world becomes more chaotic and interconnected with multiple disruptive shocks. Organisational excellence challenges the inflexibilities of Newtonian mindsets, recognising the paramount importance of interactions and further underlining the significance of invisible elements such as human potentiality, motivation and values that formulate the principles of organisational excellence. Originality/value The paper investigates the notions of quality, resilience and sustainability and their relation to motivation and organisational excellence within the framework of business management and TQM. A world-leading company – LEGO – will be used to exemplify the theoretical findings together with the Japanese Motivation Training Programme case.

Germinal matrix hemorrhage (GMH) is a devastating neurodevelopmental condition affecting preterm infants, but why blood vessels in this brain region are vulnerable to rupture remains unknown. Here we show that microglia in prenatal mouse and human brain interact with nascent vasculature in an age-dependent manner and that ablation of these cells in mice reduces angiogenesis in the ganglionic eminences, which correspond to the human germinal matrix. Consistent with these findings, single-cell transcriptomics and flow cytometry show that distinct subsets of CD45 + cells from control preterm infants employ diverse signaling mechanisms to promote vascular network formation. In contrast, CD45 + cells from infants with GMH harbor activated neutrophils and monocytes that produce proinflammatory factors, including azurocidin 1, elastase and CXCL16, to disrupt vascular integrity and cause hemorrhage in ganglionic eminences. These results underscore the brain’s innate immune cells in region-specific angiogenesis and how aberrant activation of these immune cells promotes GMH in preterm infants. Chen et al. show that subtypes of immune cells in prenatal human brain promote angiogenesis in the germinal matrix. Conversely, in preterm infants, proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage.

Purpose The paper aims to investigate the relation of narcissism to leadership in business contexts and presents its impact on specific organizational variables, which are crucial for the pursuit of excellence. Narcissism constitutes a personality trait which is considered responsible for both positive and negative behavioral outcomes that impact decisions and actions. Design/methodology/approach The approach, by which this paper is structured, is theoretical and has been based on a literature review regarding narcissism (and more specifically, narcissistic leadership) in business contexts. Findings Narcissism can have positive (mainly in the short term) and/or deleterious effects both for the individual and the organization. The self-proclaimed excellence of narcissists results from a combination of their beliefs about their extreme intelligence, abilities and leadership and their high motives for reassuring their supremacy. However, the behavior of narcissistic leaders impacts various organizational parameters which are preconditions for organizational excellence. Research limitations/implications The study at hand can be used as an input for further research on the effects of narcissism on quality performance variables. Practical implications The behavior of narcissists (and its positive and/or negative consequences) should be evaluated in relation to any specific organizational context and circumstances. Originality/value The study examines narcissistic leadership and attempts to examine its implications for factors that are crucial for the achievement of organizational excellence.

PurposeThe evolution of management underlines the importance of the human, systemic, technological and contingency element and their interaction along with an amplified awareness of organizations for achieving excellence. This paper aims to discuss whether the fusion of digital, biological and physical world leads to a new excellence perspective and to investigate the potential value of informative neuroscientific findings for setting the foundations for smart services.Design/methodology/approachThis study is based on a literature review regarding the advances of neurosciences and its implications for business. Their usefulness and potential contribution for the provision of smart services are investigated.FindingsThe fusion of technological evolution and biological sciences may potentially give birth to a new excellence conceptualization complemented by genetic data whose consequences are hard to predict. Neurosciences offer insights for various human behavior areas that can be used by business practitioners, to refine their thinking and management style and build brain-friendly organizational contexts. The combination of using neuroscientific evidence and technology in service systems sets the foundations for an “intelligent” provision of services in a quality context.Originality/valueThe paper investigates the conceptual development of excellence within the receding context of the “smart era” and the potential contribution of neurosciences for the provision of smart services with reference to quality pioneers’ theories and ideas.

Purpose The purpose of this paper is twofold: first, to examine the level of narcissism and its individual traits in students who study business, in the particular context of a regional country such as Greece; and, second, to test how several demographic variables are related to narcissism levels. Design/methodology/approach The study consists of a theoretical part on narcissism in business education and an empirical part that was based on a survey conducted with the use of a questionnaire. The analysis includes hypothesis testing and basic statistical tests. Findings Findings suggest that sex, study levels, years of business experience and (personal/family) income do impact specific narcissistic dimensions, which may be a cause for concern both for employers and higher education providers. Research limitations/implications The study was conducted in a regional country, the participants were students of public higher education institutions only and the questionnaire was self-reported, which could lead to likely social desirability effects. Practical implications The investigation of narcissism in the Greek business education might be of interest to business education providers (for providing curriculum that help future managers/leaders to deploy the positive characteristics of narcissism and avoid or not to develop the negative ones) and to future employers to apply more effective human resource practices, i.e. selection, training, rewarding. Originality/value The study at hand aimed to investigate the presence of narcissism and its individual (narcissistic) behavioral dimensions in students studying business in Greece.

Glomerular filtration rate is a key physiologic variable with a central role in clinical decision making and a strong association with prognosis in diverse populations. Reduced estimated glomerular filtration rate (eGFR) is common among adults with congenital heart disease (ACHD). We conducted a prospective cohort study of outpatient ACHD ≥18 years old seen in 2012-2017. Creatinine and cystatin C were measured; eGFR was calculated using either the creatinine or cystatin C Chronic Kidney Disease–Epidemiology Collaboration equation (CKD-EPICr and CKD-EPICysC, respectively). Survival analysis was performed to define the relationship between eGFR and both all-cause mortality and a composite outcome of death or nonelective cardiovascular hospitalization. Our cohort included 911 ACHD (39 ± 14 years old, 49% female). Mean CKD-EPICr and CKD-EPICysC were similar (101 ± 20 vs 100 ± 23 mL/min/1.73 m2), but CKD-EPICr estimates were higher for patients with a Fontan circulation (n = 131, +10 ± 19 mL/min/1.73 m2). After mean follow-up of 659 days, 128 patients (14.1%) experienced the composite outcome and 31 (3.4%) died. CKD-EPICysC more strongly predicted all-cause mortality (eGFR <60 vs >90 mL/min/1.73 m2: CKD-EPICysC unadjusted HR = 20.2 [95% CI 7.6-53.1], C-statistic = 0.797; CKD-EPICr unadjusted HR = 4.6 [1.7-12.7], C-statistic = 0.620). CKD-EPICysC independently predicted the composite outcome, whereas CKD-EPICr did not (CKD-EPICysC adjusted HR = 3.0 [1.7-5.3]; CKD-EPICr adjusted HR = 1.5 [0.8-3.1]). Patients reclassified to a lower eGFR category by CKD-EPICysC, compared with CKD-EPICr, were at increased risk for the composite outcome (HR = 2.9 [2.0-4.3], P < .0001); those reclassified to a higher eGFR class were at lower risk (HR = 0.5 [0.3-0.9], P = .03). Cystatin C–based eGFR more strongly predicts clinical events than creatinine-based eGFR in ACHD. Creatinine-based methods appear particularly questionable in the Fontan circulation.

Purpose - The aim of this paper is to contradict past and present perceptions of excellence in business education, and to present excellence as a primary element for future business education in an era of new challenges for global management.Design methodology approach - The approach, by which this paper is structured, is theoretical and has been based on literature regarding excellence in business education and philosophical works.Findings - The findings of the paper support the view that current business education results in a partial conception or misconception of management with immediate consequences in managerial decision making and business activity. Business education should be seen as an odyssey towards personal (balanced spiritual and technocratic) advancement that can pave the way for noble management behaviour.Practical implications - The paper brings out an alternative perspective on business education by considering economics and management as part of a unified context. The attainment of excellence in business activity signifies necessary changes in the foundations of business education, so that future managers have a wider and diversified knowledge repository that will allow them to act in a harmonious way.Originality value - The paper offers an alternative way of viewing excellence in business education combining knowledge on management and philosophy which perhaps needs to be taken into consideration by business schools worldwide in order to build a mentality of excellence in business behaviour.

Purpose The evolution of management underlines the importance of the human, systemic, technological and contingency element and their interaction along with an amplified awareness of organizations for achieving excellence. This paper aims to discuss whether the fusion of digital, biological and physical world leads to a new excellence perspective and to investigate the potential value of informative neuroscientific findings for setting the foundations for smart services. Design/methodology/approach This study is based on a literature review regarding the advances of neurosciences and its implications for business. Their usefulness and potential contribution for the provision of smart services are investigated. Findings The fusion of technological evolution and biological sciences may potentially give birth to a new excellence conceptualization complemented by genetic data whose consequences are hard to predict. Neurosciences offer insights for various human behavior areas that can be used by business practitioners, to refine their thinking and management style and build brain-friendly organizational contexts. The combination of using neuroscientific evidence and technology in service systems sets the foundations for an “intelligent” provision of services in a quality context. Originality/value The paper investigates the conceptual development of excellence within the receding context of the “smart era” and the potential contribution of neurosciences for the provision of smart services with reference to quality pioneers’ theories and ideas.

A bstract A search is presented for additional scalar (H) or pseudoscalar (A) Higgs bosons decaying to a top quark pair in proton-proton collisions at a center-of-mass energy of 13 TeV. The data set analyzed corresponds to an integrated luminosity of 35.9 fb − 1 collected by the CMS experiment at the LHC. Final states with one or two charged leptons are considered. The invariant mass of the reconstructed top quark pair system and variables that are sensitive to the spin of the particles decaying into the top quark pair are used to search for signatures of the H or A bosons. The interference with the standard model top quark pair background is taken into account. A moderate signal-like deviation compatible with an A boson with a mass of 400 GeV is observed with a global significance of 1.9 standard deviations. New stringent constraints are reported on the strength of the coupling of the hypothetical bosons to the top quark, with the mass of the bosons ranging from 400 to 750 GeV and their total relative width from 0.5 to 25%. The results of the search are also interpreted in a minimal supersymmetric standard model scenario. Values of m A from 400 to 700 GeV are probed, and a region with values of tan β below 1.0 to 1.5, depending on m A , is excluded at 95% confidence level.