Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
25,246
result(s) for
"Lu, Feng"
Sort by:
The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling
Atherosclerosis is a progressive, chronic inflammation in arterial walls. Long noncoding RNAs (lncRNAs) participate in inflammation, but the exact mechanism in atherosclerosis is unclear. Our microarray analyses revealed that the levels of lncRNA-FA2H-2 were significantly decreased by oxidized low-density lipoprotein (OX-LDL). Bioinformatics analyses indicated that mixed lineage kinase domain-like protein (MLKL) might be regulated by lncRNA-FA2H-2. In vitro experiments showed that lncRNA-FA2H-2 interacted with the promoter of the MLKL gene, downregulated MLKL expression, and the binding sites between −750 and 471 were necessary for lncRNA-FA2H-2 responsiveness to MLKL. Silencing lncRNA-FA2H-2 and overexpression of MLKL could activate inflammation and inhibited autophagy flux. Both lncRNA-FA2H-2 knockdown and overexpression of MLKL could significantly aggravate inflammatory responses induced by OX-LDL. We found that the 3-methyladenine (3-MA) and Atg7-shRNA enhanced inflammatory responses induced by knockdown of lncRNA-FA2H-2 and overexpression of MLKL. We demonstrated that the effects of MLKL on autophagy might be associated with a mechanistic target of rapamycin (mTOR)-dependent signaling pathways. In vivo experiments with apoE knockout mice fed a western diet demonstrated that LncRNA-FA2H-2 knockdown decreased microtubule-associated expression of microtubule-associated protein 1 light chain 3 II and lysosome-associated membrane protein 1, but increased expression of sequestosome 1 (p62), MLKL, vascular cell adhesion molecule-1, monocyte chemoattractant protein-1, and interleukin-6 in atherosclerotic lesions. Our findings indicated that the lncRNA-FA2H-2-MLKL pathway is essential for regulation of autophagy and inflammation, and suggested that lncRNA-FA2H-2 and MLKL could act as potential therapeutic targets to ameliorate atherosclerosis-related diseases.
Journal Article
Online shopping consumer perception analysis and future network security service technology using logistic regression model
In order to understand consumer perception, reduce risks in online shopping, and maintain online security, this study employs data envelopment analysis (DEA) to confirm the relationship between evaluation and stimuli. It establishes a model of stimuli-organism response and uses regression analysis to explore the relationships among negative online shopping evaluations, consumer perception of risk, and consumer behavior. This study employs attribution theory to analyze the impact of evaluations on consumer behavior and assesses the role of perceived risk as a mediator. The independent variable is negative comments, the dependent variable is consumer behavior, and logistic regression is used to empirically analyze the factors influencing online shopping security. The results indicate a positive correlation between the number of negative comments and consumers’ delayed purchase behavior, with a correlation coefficient of 41%. The intensity of negative comments significantly impacts consumers’ refusal to make a purchase, with a correlation coefficient of 38%. The length of negative comments substantially influences consumers’ opposition to purchasing, also with a correlation coefficient of 38%. There is a close relationship between perceived risk and consumers’ delayed shopping behavior and the number of negative comments, with 41% and 4% correlation coefficients, respectively. Perceived risk has a relatively smaller impact on consumers’ opposition to purchase behavior, with a correlation coefficient of 27%. The length, intensity, and number of negative comments are correlated with consumers’ opposition, refusal, and delayed consumption, negatively affecting consumer intent. Additionally, negative comments are related to perceived risk and consumer behavior. Perceived risk causally influences consumer behavior, while the convenience of shopping has a relatively minor impact on online shopping security. Factors like delivery speed, buyer reviews, brand, price, and consumer perception are significantly related to online shopping security. Consumer perception has the most significant impact on online shopping security, balancing secure and fast consumption under the guarantee of user experience. Strengthening consumer perception enhances consumers’ ability to process risk information, helping them better identify risks and avoid using hazardous network software, tools, or technologies, thereby reducing potential online security risks.
Journal Article
A microRNA-inducible CRISPR–Cas9 platform serves as a microRNA sensor and cell-type-specific genome regulation tool
microRNAs (miRNAs) are small noncoding RNAs that play important regulatory roles in plants, animals and viruses. Measuring miRNA activity in vivo remains a big challenge. Here, using an miRNA-mediated single guide RNA (sgRNA)-releasing strategy and dCas9–VPR to drive a transgene red fluorescent protein, we create an miRNA sensor that can faithfully measure miRNA activity at cellular levels and use it to monitor differentiation status of stem cells. Furthermore, by designing sgRNAs to target endogenous loci, we adapted this system to control the expression of endogenous genes or mutate specific DNA bases upon induction by cell-type-specific miRNAs. Finally, by miRNA sensor library screening, we discover a previously undefined layer of heterogeneity associated with miR-21a activity in mouse embryonic stem cells. Together, these results highlight the utility of an miRNA-induced CRISPR–Cas9 system as miRNA sensors and cell-type-specific genome regulation tools.
Wang et al. developed an inducible CRISPR–Cas9 system, in which guide RNA release is controlled by specific microRNAs, and demonstrated its application as a microRNA sensor and cell-type-specific genome regulator.
Journal Article
Hydrogenated Cs2AgBiBr6 for significantly improved efficiency of lead-free inorganic double perovskite solar cell
Development of lead-free inorganic perovskite material, such as Cs
2
AgBiBr
6
, is of great importance to solve the toxicity and stability issues of traditional lead halide perovskite solar cells. However, due to a wide bandgap of Cs
2
AgBiBr
6
film, its light absorption ability is largely limited and the photoelectronic conversion efficiency is normally lower than 4.23%. In this text, by using a hydrogenation method, the bandgap of Cs
2
AgBiBr
6
films could be tunable from 2.18 eV to 1.64 eV. At the same time, the highest photoelectric conversion efficiency of hydrogenated Cs
2
AgBiBr
6
perovskite solar cell has been improved up to 6.37% with good environmental stability. Further investigations confirmed that the interstitial doping of atomic hydrogen in Cs
2
AgBiBr
6
lattice could not only adjust its valence and conduction band energy levels, but also optimize the carrier mobility and carrier lifetime. All these works provide an insightful strategy to fabricate high performance lead-free inorganic perovskite solar cells.
Though inorganic perovskites are an attractive, non-toxic and stable alternative to organic-inorganic halide perovskite solar cells, realizing efficient devices remains a challenge. Here, the authors report hydrogenated lead-free inorganic perovskite solar cells with enhanced power conversion efficiency.
Journal Article
Beating the Fundamental Rate-Distance Limit in a Proof-of-Principle Quantum Key Distribution System
With the help of quantum key distribution (QKD), two distant peers are able to share information-theoretical secure key bits. Increasing the key rate is ultimately significant for the applications of QKD in the lossy channel. However, it has been proven that there is a fundamental rate-distance limit, called the linear bound, which restricts the performance of all existing repeaterless protocols and realizations. Surprisingly, a recently proposed protocol, called twin-field (TF) QKD, can beat the linear bound with no need for quantum repeaters. Here, we present one of the first implementations of the TF-QKD protocol and demonstrate its advantage of beating the linear bound at a channel distance of 300 km. In our experiment, a modified TF-QKD protocol that does not assume phase postselection is considered, and thus a higher key rate than the original one is expected. After controlling the phase evolution of the twin fields traveling through hundreds of kilometers of optical fibers, the implemented system achieves high-visibility single-photon interference and allows stable and high-rate measurement-device-independent QKD. Our experimental demonstration and results confirm the feasibility of the TF-QKD protocol and its prominent superiority in long-distance key distribution services.
Journal Article
Aging and homeostasis of the hypodermis in the age-related deterioration of skin function
Adipose tissues in the hypodermis, the crucial stem cell reservoir in the skin and the endocrine organ for the maintenance of skin homeostasis undergo significant changes during skin aging. Dermal white adipose tissue (dWAT) has recently been recognized as an important organ for both non-metabolic and metabolic health in skin regeneration and rejuvenation. Defective differentiation, adipogenesis, improper adipocytokine production, and immunological dissonance dysfunction in dWAT lead to age-associated clinical changes. Here, we review age-related alterations in dWAT across levels, emphasizing the mechanisms underlying the regulation of aging. We also discuss the pathogenic changes involved in age-related fat dysfunction and the unfavorable consequences of accelerated skin aging, such as chronic inflammaging, immunosenescence, delayed wound healing, and fibrosis. Research has shown that adipose aging is an early initiation event and a potential target for extending longevity. We believe that adipose tissues play an essential role in aging and form a potential therapeutic target for the treatment of age-related skin diseases. Further research is needed to improve our understanding of this phenomenon.
Journal Article
Porous flexible molecular-based piezoelectric composite achieves milliwatt output power density
Molecular ferroelectrics have made breakthrough progress in intrinsic piezoelectric response that can be on par with advanced inorganic piezoelectric ceramics. However, their successful applications in high-density energy harvesting and self-powered flexible devices have been great challenge, owing to the low elastic moduli, intrinsically brittle, and fracture proneness of such material systems under mechanical loading. Here, we have developed a flexible porous composite piezoelectric material by using soft thermoplastic polyurethane (TPU) and molecular ferroelectric materials. Benefiting from the porous structure of TPU, the flexible piezoelectric composites enable effectively large doping ratio (50%) of [Me
3
NCH
2
Cl]CdCl
3
(TMCM-CdCl
3
) and highly efficient stress absorption, coupled with the excellent piezoelectric properties of TMCM-CdCl
3
, to realize a superior power density (636.9 µW cm
−2
or 1273.9 µW cm
−3
). This output is 2000 times higher than that of flexible piezoelectric materials represented by poly(vinylidene fluoride) (PVDF). We believe that the outstanding performance of the porous composite piezoelectric material would pave a feasible way for real industrial applications of molecular ferroelectrics.
This work presents a flexible porous composite piezoelectric material combining thermoplastic polyurethane and TMCM-CdCl
3
, achieving near milliwatt power density, with promising industrial application prospects.
Journal Article
The cortical regions and white matter tracts underlying auditory comprehension in patients with primary brain tumor
The comprehension of spoken language is one of the most essential language functions in humans. However, the neurological underpinnings of auditory comprehension remain under debate. Here we used multi‐modal neuroimaging analyses on a group of patients with low‐grade gliomas to localize cortical regions and white matter tracts responsible for auditory language comprehension. Region‐of‐interests and voxel‐level whole‐brain analyses showed that cortical areas in the posterior temporal lobe are crucial for language comprehension. The fiber integrity assessed with diffusion tensor imaging of the arcuate fasciculus and the inferior longitudinal fasciculus was strongly correlated with both auditory comprehension and the grey matter volume of the inferior temporal and middle temporal gyri. Together, our findings provide direct evidence for an integrated network of auditory comprehension whereby the superior temporal gyrus and sulcus, the posterior parts of the middle and inferior temporal gyri serve as auditory comprehension cortex, and the arcuate fasciculus and the inferior longitudinal fasciculus subserve as crucial structural connectivity. These findings provide critical evidence on the neural underpinnings of language comprehension. Our findings provide direct evidence for an integrated network of auditory comprehension whereby not only the superior temporal gyrus and sulcus but the posterior parts of the middle and inferior temporal gyri also serve as auditory comprehension cortex, and the arcuate fasciculus and the inferior longitudinal fasciculus subserve as crucial structural connectivity. These findings provide new evidence on the neural underpinnings of language comprehension
Journal Article
Compressive Response and Damage Distribution of Fiber-Reinforced Concrete with Various Saturation Degrees
Tunnels frequently experience issues such as lining spalling and water leakage, making the stability of tunnel support critical for engineering safety. Given that tunnels are subjected to various ground stress disturbances and groundwater influences, it is essential to investigate the mechanical properties and damage mechanisms of tunnel support materials under different loading paths and saturation levels. Fiber-reinforced concrete (FRC) is widely used for tunnel support; in this study, uniaxial compression tests were conducted on FRC with different fiber contents (0%, 0.5%, 1.0%) under varying loading paths (monotonic, pre-peak cyclic loading, full cyclic loading). The stress–strain behavior, volumetric strain, and elastic modulus were analyzed. The results indicate that increasing fiber content enhances strength and stiffness, while higher water content leads to a significant water-weakening effect, reducing both parameters. To classify crack types, the logistic regression (LR) algorithm is employed based on the AF-RA features, identifying tensile damage (which accounts for 60–80%) as more dominant than shear damage. Using this classification, AE event distributions reveal the spatial characteristics of internal damage in FRC. Gaussian process regression (GPR) is further applied to predict the AE parameters, enabling the assessment of the tensile and shear damage responses in FRC. The location and magnitude of the predicted wave crest indicate extreme damage levels, which become more pronounced under a higher saturation condition. A damage constitutive model is proposed to characterize the post-peak softening behavior of FRC. The numerical verification demonstrates good agreement with the experimental results, confirming the model’s capability to describe the softening behavior of FRC under various fiber and water contents.
Journal Article