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Extending product life is one of the effective ways to reduce the waste of resources. However, many unsatisfactory products are scrapped because of a lack of adequate performance. The product should be improved and upgraded innovatively, and the existing upgradable products may create more economic benefits for the longer product life cycles. This paper proposed a product innovative design and product upgrade employing an Extenics-TRIZ Integrated requirement-function-principle-structure (RFPS) model, which aims at complex requirement flexibility with easy-to-use design process when the product needs a redesign. Here, the requirement flexibility refers to the ability of a design object to adapt its design levels. There are two design strategies: the extension analysis methods are utilized to map the top-level requirements to functions, principles, and structures requirements, and then the TRIZ is used to handle the design problems according to the objects on different levels. This design knowledge is summarized as RFPS, and it can be reused in computer-aided innovation further. A case study for a cutting table is illustrated to the innovation and upgrade, and it indicates the effectiveness for designers to implement the design methodology.

Stroke is the result of blockage or rupture of blood vessels in the brain and is the leading cause of death and disability in the world. Currently only a very limited number of therapeutic approaches are available for treatment of stroke patients, and the vast majority of neuroprotective agents that tested positively in pre-clinical studies failed in clinical trials. In recent years, the clinical value of the use of exosomes for stroke treatment has received widespread attention due their unique characteristics such as low immunogenicity, low toxicity and biodegradability, ability to cross the blood–brain barrier (BBB), and their important role in communication between cells. More and more evidence suggests that the secretion of exosomes is the mechanism underlying the protection induced by mesenchymal stromal cells (MSCs) after stroke. Exosomes are thought to support brain restoration and induce repairing effects, including neurovascular remodeling, and anti-apoptosis and anti-inflammatory effects. Recent reports have focused on the clinical application of exosomes as a potential drug delivery approach. This review focuses on the ability of exosomes to interrupt the stroke-induced pathologic processes of stroke, and on publications describing how to achieve more effective treatment of stroke with exosomes.

Adult neurogenesis mainly occurs at the subventricular zone (SVZ) on the walls of the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus (DG). However, the majority of newborn neurons undergo programmed cell death (PCD) during the period of proliferation, migration, and integration. Stroke activates neural stem cells (NSCs) in both SVZ and SGZ. This process is regulated by a wide variety of signaling pathways. However, the newborn neurons derived from adult neurogenesis are insufficient for tissue repair and function recovery. Thus, enhancing the endogenous neurogenesis driven by ischemia and promoting the survival of newborn neurons can be promising therapeutic interventions for stroke. Here, we present an overview of the process of adult neurogenesis and the potential of stroke-induced neurogenesis on brain repair.

Procambarus clarkii is an important economic aquaculture species worldwide. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) infects numerous crustacean hosts, including P. clarkii. However, there have been few reports on the prevalence of IHHNV in P. clarkii. In this study, 200 farmed P. clarkii were collected from Anhui, Jiangsu, Zhejiang, Hunan, Hubei, and Sichuan provinces in China. PCR detection was employed per the protocol by the World Organization for Animal Health (WOAH) to identify and detect the presence of IHHNV. The positive rate of IHHNV in different provinces ranged from 16.7 to 56.7%, and the overall IHHNV-positive rate was 38.5%. IHHNV strains isolated in this study related closely to infectious IHHNV and split into two major distinct branches. Besides, the IHHNV strains shared a high homology (93.4–99.4%). These findings suggest that a high prevalence of IHHNV was established in farmed P. clarkii in the middle and lower reaches of the Yangtze River.

Ganglioside GM1, which is particularly abundant in the central nervous system (CNS), is closely associated with the protection against several CNS disorders. However, controversial findings have been reported on the role of GM1 following ischemic stroke. In the present study, using a rat middle cerebral artery occlusion (MCAO) model, we investigated whether GM1 can protect against ischemic brain injury and whether it targets the autophagy pathway. GM1 was delivered to Sprague-Dawley male rats at 3 doses (25 mg/kg, 50 mg/kg, 100 mg/kg) by intraperitoneal injection soon after reperfusion and then once daily for 2 days. The same volume of saline was given as a control. Tat-Beclin-1, a specific autophagy inducer, was administered by intraperitoneal injection at 24 and 48 hours post-MCAO. Infarction volume, mortality and neurological function were assessed at 72 hours after ischemic insult. Immunofluorescence and Western blotting were performed to determine the expression of autophagy-related proteins P62, LC3 and Beclin-1 in the penumbra area. No significant changes in mortality and physiological variables (heart rate, blood glucose levels and arterial blood gases) were observed between the different groups. However, MCAO resulted in enhanced conversion of LC3-I into LC3-II, P62 degradation, high levels of Beclin-1, a large area infarction (26.3±3.6%) and serious neurobehavioral deficits. GM1 (50 mg/kg) treatment significantly reduced the autophagy activation, neurobehavioral dysfunctions, and infarction volume (from 26.3% to 19.5%) without causing significant adverse side effects. However, this biological function could be abolished by Tat-Beclin-1. GM1 demonstrated safe and robust neuroprotective effects that are associated with the inhibition of autophagy following experimental stroke.

Microwave-assisted rock breaking is recognized as an effective technology for reducing tool wear and enhancing rock-breaking efficiency. In this study, basalt rock was irradiated with a microwave power of 3 kW and 6 kW for 30 s, 60 s, 90 s, and 120 s. Subsequently, uniaxial compression, uniaxial loading and unloading, and acoustic emission tests were performed. The damage evolution of basalt was assessed through non-destructive acoustic testing methods and nuclear magnetic resonance (NMR) techniques. The results showed that the P-wave velocity, uniaxial compressive strength (UCS), and modulus of elasticity (E) exhibited varying degrees of deterioration as the microwave radiation time increased. An increase in microwave radiation time and power led to heightened acoustic emission activity in basalt and a significant rise in the proportion of shear cracks during uniaxial compression. From an energy perspective, microwave irradiation decreased the energy storage capacity of the basalt specimen prior to its peak point, with increased power and duration. At a microscopic level, porosity and the macroporous fractal dimension increased with extended microwave radiation time and power, indicating that microwave irradiation facilitated the growth of larger fractal pore structures. The findings of this study offer scientific insights for the application of microwave-assisted rock crushing.

Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis (HVHN) disease, which causes serious economic losses in the crucian carp culture industry. In this study, by displaying ORF132 on the surface of Saccharomyces cerevisiae cells (named EBY100/pYD1-ORF132), we evaluated the protective efficacy of oral administration against CyHV-2 infection. Intense innate and adaptive immune responses were evoked in both mucosal and systemic tissues after oral vaccination with EBY100/pYD1-ORF132. Importantly, oral vaccination provided significant protection for crucian carp post CyHV-2 infection, resulting in a relative percent survival (RPS) of 64%. In addition, oral administration suppressed the virus load and relieved histological damage in selected tissues. Our results indicated that surface-displayed ORF132 on S. cerevisiae could be used as potential oral vaccine against CyHV-2 infection.

The rice leaf-roller Cnaphalocrocis medinalis is an important migratory pest of rice. We conducted a study to determine the physiological status of adults trapped by a sex pheromone and floral odor. In the immigrant group, the number of males trapped by the floral odor was greater than the number caught by sex pheromone trapping. The volume of testes was similar in the above two trapping methods but was smaller than in the sweep net method. The ovary developmental grade, mating rate, and number of matings of females caught in floral odor trap were higher than in those caught in the sweep net. In the local breeding group, the number of males trapped by sex pheromones was greater than the number trapped by the floral odor. The volume of testes was smaller in the floral odor trap compared to the pheromone trap group, with the largest in the sweep net group. The ovarian developmental grade, mating rate, and number of matings of females were significantly higher in the floral odor trap group than in the sweep net group. In the emigrant group, the adult olfactory response to the sex pheromone and floral odor was low. The volume of testes was larger in the sweep net group compared to the moths caught by floral odor trapping. The number of eggs laid by female immigrants trapped by the floral odor and sweep net was similar, while the number in the local breeding group was greater in moths caught with the sweep net in comparison with those caught by the floral odor trap. The difference in egg hatchability between the two trapping methods in both immigrants and local breedings was not significant.

The safe and efficient mining of deep coal resources is severely restricted by the dynamic disasters caused by high gas and high ground stress. Taking a deep mine in China as the research background, a mechanical model of the front supporting stress with the working face was constructed through theoretical calculations. Based on the limit equilibrium theory, the stress distribution in the plastic zone and the elastic zone of the lateral working face was derived; based on semi-infinite plane mechanics model, the floor vertical and horizontal stress distribution was deduced. Then, the roadway surrounding rock stress and displacement field distribution evolution characteristics were revealed through numerical simulation. On this basis, the reasonable floor gas drainage roadway (FGDR) layout was determined: internal staggered layout was used with FGDR, the vertical distance to the working face is 20 m, and the horizontal distance to the working face end is 15 m; the open-off cut of FGDR was arranged in an external staggered layout, the vertical distance to the working face is 20 m, and the horizontal distance to the open-off cut of the working face is 15 m. It is an important practical significance for the layout of FGDR, the control of surrounding rocks, and the improvement of gas drainage effects under similar conditions through the research results.

The present study was conducted to evaluate the effect of dietary Silphium perfoliatum L. on growth performance, immunity, intestinal health, and resistance against Nocardia seriolae of largemouth bass. Four dietary concentrations of S. perfoliatum L. were prepared: 0% (SPL0), 1% (SPL1), 2.5% (SPL2.5), and 5% (SPL5). After 60 d feeding, a challenge test was conducted by injecting N. seriolae intraperitoneally. The results showed that supplementing the diets with S. perfoliatum L. could promote the weight gain rate, specific growth rate, and feed intake. Meantime, S. perfoliatum L. could also enhance the activity of immune-related enzymes and improve the expression of immune-related genes in the liver. Compared to the control group, S. perfoliatum L. improved intestinal morphology and reduced the abundance of harmful bacteria in intestinal microbiota. Importantly, supplementing the diets with S. perfoliatum L. protected largemouth bass from N. seriolae infection, resulting in a relative percent survival (RPS) of 24% (SPL1), 31.99% (SPL2.5), and 43.99% (SPL5). In conclusion, the present study showed that S. perfoliatum L. could boost growth performance, enhance immunity, promote intestinal health, and improve the ability of largemouth bass against N. seriolae infection.