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Previous studies have shown that baicalin, an active ingredient of the Chinese traditional medicine Huangqin, attenuates LPS-induced inflammation by inhibiting the activation of TLR4/NF-κBp65 pathway, but how it affects this pathway is unknown. It has been shown that CD14 binds directly to LPS and plays an important role in sensitizing the cells to minute quantities of LPS via chaperoning LPS molecules to the TLR4/MD-2 signaling complex. In the present study we investigated the role of CD14 in the anti-inflammatory effects of baicalin in vitro and in vivo. Exposure to LPS (1 μg/mL) induced inflammatory responses in RAW264.7 cells, evidenced by marked increases in the expression of MHC II molecules and the secretion of NO and IL-6, and by activation of MyD88/NF-κB p65 signaling pathway, as well as the expression of CD14 and TLR4. These changes were dose-dependently attenuated by pretreatment baicalin (12.5–50 μM), but not by baicalin post-treatment. In RAW264.7 cells without LPS stimulation, baicalin dose-dependently inhibit the protein and mRNA expression of CD14, but not TLR4. In RAW264.7 cells with CD14 knockdown, baicalin pretreatment did not prevent inflammatory responses and activation of MyD88/NF-κB p65 pathway induced by high concentrations (1000 μg/mL) of LPS. Furthermore, baicalin pretreatment also inhibited the expression of CD14 and activation of MyD88/NF-κB p65 pathway in LPS-induced hepatocyte-derived HepG2 cells and intestinal epithelial-derived HT-29 cells. In mice with intraperitoneal injection of LPS and in DSS-induced UC mice, oral administration of baicalin exerted protective effects by inhibition of CD14 expression and inflammation. Taken together, we demonstrate that baicalin pretreatment prevents LPS-induced inflammation in RAW264.7 cells in CD14-dependent manner. This study supports the therapeutic use of baicalin in preventing the progression of LPS-induced inflammatory diseases.

A kind of intelligent ventilation system is studied in this paper, which includes host system and slave system. The design scheme and schematic diagram of the hardware circuit are given. A variety of sensors are integrated, which can measure temperature, humidity, formaldehyde, PM2.5 dust, smoke and other parameters. According to the measured data, the host sends out control commands such as “open window” ” purify air” and “draught fan speed”, and realizes intelligent ventilation. The system has dust prevention, damp proofing and other work mode. The power drive circuit designed by SCR designs to realize no spark control. Host monitors multiple slave stations using ZigBee wireless networks, through the WIFI broadband access network and realize the application of “Internet +”. Therefore, users can view and monitor the home environment status through the mobile phone APP software. The system can be used in the newly decorated houses without ventilation, and can also be used in various mines, subway stations, working room of chemical plant and other workplaces

Necroptosis is a new type of programmed cell death discovered in recent years, playing an important role in various diseases. Since it was conceptualized in 2005, research on necroptosis has developed rapidly. However, few bibliometric analyses have provided a comprehensive overview of the field. This study aimed to employ a bibliometric analysis to assess necroptosis research’s current status and hotspot, highlight landmark findings, and orientate future research. A total of 3993 publications from the WoSCC were collected for this study. Multiple tools were used for bibliometric analysis and data visualization, including an online website, VOSviewer, CiteSpace, and HistCite. Publications related to necroptosis have increased significantly annually, especially in the last 5 years. Globally, the USA and Harvard University are the most outstanding countries and institutions in this field, respectively. The academic groups managed by Peter Vandenabeele and Junying Yuan both have permanent and intensive research on necroptosis. Cell Death and Differentiation is the most vital journal in this field. The molecular mechanisms of necroptosis and its role in disease are the focus of current research, while the crosstalk between programmed cell death is an emerging direction in the field. The “reactive oxygen species”, “innate immunity”, and “programmed cell death” may be potential research hotspots. Our results present a comprehensive knowledge map and explore research trends. Researchers and funding agencies on necroptosis can obtain helpful references from our study.

Bilophila wadsworthia is rarely found in the bloodstream. We report the first case of bloodstream infection caused by B. wadsworthia in a diabetic foot patient in China. B. wadsworthia is a normal component of the human intestinal microbiota, and a history of diarrhea may contribute to its translocation into the bloodstream. Due to the slow growth of B. wadsworthia , prolonged incubation was essential for its detection. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) enabled rapid identification of the pathogen. Early initiation of Piperacillin/Tazobactam therapy resulted in a favorable clinical outcome.

Malachite green (MG) residue in aquatic products is a widely concerning issue, and the possible source of MG contamination includes its illegal usage and environmental pollution. A variety of strategies for solving such a problem have been proposed, and the research about them is summarized in this review. The MG contamination in aquaculture environments can be eliminated by adsorption, degraded by advanced oxidation processes (AOPs), or biodegraded by microbes or enzymes. The illegal usage of MG can be prevented by screening novel anti-Saprolegnia sp. agents from current available agricultural antibiotics, plant extracts, or antagonistic microbes. Nevertheless, deficiencies also existed in these proposed solving strategies. Therefore, further research opportunities in such areas were provided. This includes developing effective combinatorial methods (adsorption + AOPs or biodegradation) for eliminating MG from the aquaculture environment; systematically considering the impact of practical conditions on the efficiency of MG elimination; screening more efficient anti-Saprolegnia sp. agents; and systematically evaluating both the in vivo activities and safety of these agents.

The aim of the present study was to investigate whether fraction from Lycium barbarum polysaccharide (LBP) could reduce immunotoxicity and enhance antitumor activity of doxorubicin (Dox) in mice. A water-soluble LBP fraction, designated LBP3, was isolated from edible Chinese herbal Lycium barbarum and used in this study. To investigate the effect of LBP3 on Dox-induced immunotoxicity, tumor-free mice were used and treated with either normal saline, Dox, or Dox plus LBP3. To investigate the effect of LBP3 on antitumor activity of Dox, H22 tumor-bearing mice were used and treated with either normal saline, Dox, LBP3, or Dox plus LBP3. The results showed that LBP3 did not protect against the body weight loss caused by Dox, but it promoted the recovery of body weight starting at day 5 after Dox treatment in tumor-free mice. LBP3 also improved peripheral blood lymphocyte counts, promoted cell cycle recovery in bone marrow cells, and restored the cytotoxicity of natural killer cells. Furthermore, in H22 tumor-bearing mice, LBP3 enhanced antitumor activity of Dox and improved peripheral blood lymphocyte counts and the cytotoxicity of splenocytes. In brief, our results demonstrated that LBP3 could reduce the immunotoxicity and enhance antitumor activity of Dox.

As one of the ligands of aryl hydrocarbon receptor (AhR), baicalein, isolated from Scutellaria baicalensis Georgi, has been proved to exert potential therapeutic effects on ulcerative colitis (UC), but its therapeutic mechanism remains obscure. Authentically, ulcerative colitis can be alleviated by regulating the differentiation of naïve CD4+ T cells via AhR activation. So, our study planned to prove the hypothesis that baicalein protected mice against UC by regulating the balance of Th17/Treg cells via AhR activation. Immunofluorescence and western blot results showed that baicalein could promote AhR activation and induce it to transfer to the nucleus. We further determined the effect of baicalein on naïve CD4+ T cell differentiation in vitro by magnetic cell separation and drug intervention. The results showed that baicalein could promote Treg cell differentiation by activating AhR. In vivo study, UC mice were established by free drinking of dextran sulfate sodium (DSS) for 7 days and then were orally administrated by baicalein (10, 20, and 40 mg/kg), TCDD (AhR agonist), and CH223191 (antagonist). The results demonstrated that baicalein improved the symptoms of UC mice, regulated the balance of Th17/Treg cells, and restored the balance of proinflammatory cytokines such as IL-17, IL-6, and TNF-α; anti-inflammatory cytokines such as IL-10 and TGF-β; and epithelial protective cytokine IL-22 in UC mice, and these effects were related to AhR. Taken together, our research found that baicalein might be a potential drug for UC via regulating Treg cell differentiation and maintaining immune homeostasis and attempted to shed a light on the pivotal role of AhR in these effects.

Objective To investigate the safety and feasibility of high intensity focused ultrasound (HIFU) ablation followed by ultrasound-guided dilation and curettage (USg-D&C) for two types patients with cesarean scar pregnancy (CSP-I and CSP-II). Materials and methods This study was a retrospective analysis of 101 CSP-I patients and 52 CSP-II patients who received HIFU ablation followed by USg-D&C from Jun 2014 to Oct 2020. The diameter of gestational sac/mass, thickness of the intervening myometrium, intraoperative blood loss, operation time, length of hospital stays, adverse effects and β-HCG level in the two groups were compared. Results All patients successfully received HIFU ablation under conscious sedation. The median total treatment time of HIFU ablation and median USg-D&C time in the CSP-I group were statistically longer than those in the CSP-II group ( P <  0.05). The average intraoperative median blood loss was 39 ml in the CSP-I group and 65 ml in the CSP-II group ( P <  0.05). The duration of hospitalization was 7.07 ± 1.83 days in the CSP-I group and 7.18 ± 1.72 days in the CSP-II group ( P >  0.05). The average time needed for β-HCG return to normal levels was 26.08 ± 5.02. and 28.15 ± 4.99 days for CSP-I and CSP-II, respectively ( P  > 0.05). The percentage of adverse effects and complications was not significantly different between the two groups ( P >  0.05). Conclusions HIFU ablation followed by USg-D&C was safe and effective in treating the CSP-I patients and CSP-II patients, which may be a potential noninvasive therapeutic option for patients with CSP.

The treatment of Sb (III) wastewater produced from mining activities is uniquely challenging and has therefore garnered increasing attention. Here, an amino-modified zirconium-based metal-organic framework material (UiO-66-NH2) and its composites were loaded onto graphene oxide (GO@UiO-66-NH2) via the hydrothermal method, after which these materials were used to adsorb Sb (III) in mine wastewater. The effects of adsorption time, pH, initial Sb (III) concentration, temperature, and adsorbent dosage on the removal performance of Sb (III) were then investigated. The adsorption processes of Sb (III) were examined via adsorption kinetic, isotherm, and thermodynamic analyses. XRD, SEM, and FTIR analyses demonstrated the presence of a porous structure and high levels of oxygen-containing functional groups on the UiO-66-NH2 and GO@UiO-66-NH2 surfaces. During the Sb (III) adsorption process, the adsorption rates of UiO-66-NH2 and GO@UiO-66-NH2 were very fast in the first 10 minutes, and the adsorption equilibrium was achieved in 12 h, with the adsorption efficiencies of 91.76% and 93.79%, respectively. At a pH of 7.0, 25°C, an initial Sb (III) concentration of 100 mg/L, and an adsorbent dosage of 0.04 g/L, the maximum Sb (III) adsorption capacities of UiO-66-NH2 and GO@UiO-66-NH2 reached 39.23 mg/g and 61.07 mg/g, respectively. The adsorption process was accurately described by the Langmuir model, meaning that the Sb (III) was adsorbed through single-layer uniform adsorption. Moreover, the adsorption process was highly consistent with the pseudo-second-order model, which was indicative of spontaneous and endothermic chemical adsorption. Additionally, the Sb (III) removal efficiency could be maintained approximately 70% after sorption-desorption recycling four times. Therefore, our study provides an economical and effective method for the removal of Sb (III) in wastewater treatment.

Recombinant adeno-associated viruses (rAAVs) have emerged as promising gene therapy vectors due to their proven efficacy and safety in clinical applications. In non-human primates (NHPs), rAAVs are administered via suprachoroidal injection at a higher dose. However, high doses of rAAVs tend to increase additional safety risks. Here, we present a novel AAV capsid (AAVv128), which exhibits significantly enhanced transduction efficiency for photoreceptors and retinal pigment epithelial (RPE) cells, along with a broader distribution across the layers of retinal tissues in different animal models (mice, rabbits, and NHPs) following intraocular injection. Notably, the suprachoroidal delivery of AAVv128-anti-VEGF vector completely suppresses the Grade IV lesions in a laser-induced choroidal neovascularization (CNV) NHP model for neovascular age-related macular degeneration (nAMD). Furthermore, cryo-EM analysis at 2.1 Å resolution reveals that the critical residues of AAVv128 exhibit a more robust advantage in AAV binding, the nuclear uptake and endosome escaping. Collectively, our findings highlight the potential of AAVv128 as a next generation ocular gene therapy vector, particularly using the suprachoroidal delivery route. In non-human primates, rAAVs are delivered through suprachoroidal injection at a high dose to achieve optimal efficacy. Here, the authors present a novel AAV capsid (AAVv128) that significantly improved the transduction efficiency in photoreceptor and retinal pigment epithelial cells across species.