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Under the influence of artificial intelligence (AI), Internet of Things (IOT) and big data technology, V2X vehicle infrastructure cooperative technology is rapidly applied to intelligent traffic management and road condition services. However, the existing roadside traffic control equipment has difficulty in data aggregation and processing, that cannot meet the application requirements of the intelligent connection and edge calculation of the V2X vehicle infrastructure cooperative device. In this paper, the \"edge computing + end-edge-cloud collaboration\" mode is used to construct the roadside intelligent information interaction system. As an intelligent connection node between roadside traffic management system and V2X vehicle infrastructure cooperative system, the design makes roadside video analysis and real-time data fusion and push function come true. Because of its lightweight intersection deployment mode, regional-level road condition diagnosis and optimization control capabilities, it has broad application prospects.

Overexpression of bacterial γ‐glutamylcysteine synthetase in the cytosol of Populus tremula × P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd) phytoremediation. However, the net Cd²⁺influx in association with H⁺/Ca²⁺, Cd tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars. We assessed net Cd²⁺influx, Cd tolerance and the transcriptional regulation of several genes involved in Cd²⁺transport and detoxification in wild‐type and transgenic poplars. Poplars exhibited highest net Cd²⁺influxes into roots at pH 5.5 and 0.1 mM Ca²⁺. Transgenics had higher Cd²⁺uptake rates and elevated transcript levels of several genes involved in Cd²⁺transport and detoxification compared with wild‐type poplars. Transgenics exhibited greater Cd accumulation in the aerial parts than wild‐type plants in response to Cd²⁺exposure. Moreover, transgenic poplars had lower concentrations of O₂˙⁻and H₂O₂; higher concentrations of total thiols, GSH and oxidized GSH in roots and/or leaves; and stimulated foliar GSH reductase activity compared with wild‐type plants. These results indicate that transgenics are more tolerant of 100 μM Cd²⁺than wild‐type plants, probably due to the GSH‐mediated induction of the transcription of genes involved in Cd²⁺transport and detoxification.

The Diego blood group is a crucial blood group system for ensuring the safety of clinical transfusions. However, current detection methods for the Diego blood group remain limited. This study aims to develop a multiplex digital PCR approach for Diego blood group genotyping and establish a blood pool in the northwest region. Additionally, multiplex digital PCR is used to determine the distribution frequencies of the Diego blood group antigens Dia and Dib in this region. The proportions of Di(a+b-), Di(a+b+), and Di(a-b+) phenotypes in a blood pool of 1000 individuals, as determined by multiplex digital PCR, were 0.281%, 6.972%, and 92.747%, respectively. The frequencies of the Dia and Dib antigens in Xi’an region were 7.253% and 99.719%, respectively. This study established a multiplex digital PCR method for detecting the Diego blood group and analyzing its population distribution frequency. The method enables accurate Diego blood group detection in individual samples and provides population-level distribution data. These findings are valuable for understanding genetic polymorphisms and heterogeneity in the local population. In this study, multiplex digital PCR was employed for the first time to detect the Diego blood group. This approach holds significant promise for being extended to a wider range of blood group research in the future, thus demonstrating extensive potential for application.

Backgrounds The oligopeptide transporter (OPT) family belongs to a type of proton-coupled symporter and functions in the transport of metal ions, glutathione, phytochelatins (PCs), and metal-PC complexes, as well as in the regulation of metal homeostasis. Polplar, a fast-growing woody model species which has been resequenced multiple times, shows great potential in phytoremediation. However, the identification of OPT family genes and their responses to cadmium (Cd) and iron (Fe) remain poorly understood. Results This study identified 30 OPT genes (17 yellow stripe-like (YSL) subfamily members and 13 OPT subfamily members) in Populus trichocarpa , and most PtOPT proteins were localized to the plasma membrane and tonoplast. Twelve gene duplication events were detected among the members of the PtOPT gene family, with one duplication pair being a tandem duplication and the remaining 11 pairs being segmental duplications. Numerous cis -acting elements related to light responses, hormone responses, stress responses, and plant development have been identified. The expression profiles of the PtOPT genes varied significantly among different tissues. Coexpression networks centered on PtOPT4.2 were constructed to reveal its potential roles in cellular nitrogen compound metabolism, protein-containing complex metabolism, small molecule metabolism, and carboxylic acid metabolism. The expression levels of PtYSL6 , PtYSL7.4 , PtOPT2 , PtOPT5.2 , and PtYSL3.3 were highly induced by Cd under normal Fe conditions, whereas the expression levels of these five genes were reduced by Cd under high Fe conditions, indicating that these five genes may play roles in the transport and distribution of Fe and Cd, as well as in Cd detoxification. Conclusions These findings enhance our understanding of the critical role of PtOPT genes in response to Fe and Cd, and provide valuable molecular targets for improving Cd phytoremediation potential.

Cryptosporidium spp. are prevalent zoonotic pathogens that affect both humans and animals. The pathogens are spread through feces and represent a major cause of diarrhea. As they are both abundant and widely distributed, wild rodents play a significant role in the transmission of Cryptosporidium spp. The Dabie Mountains in southeast Henan Province are rich in wildlife resources as well as various species of livestock. However, the epidemiological characteristics of Cryptosporidium spp. among local wild rodents remain poorly understood. Therefore, the infection rate and genetic characteristics of Cryptosporidium spp. in wild rodents within this region should be determined. Between March 2023 and April 2024, a total of 267 wild rodents were captured in the northern foothills of the Dabie Mountains, and fecal samples were collected from their intestines for DNA extraction. Species identification of wild rodents was conducted using PCR amplification of the universal vertebrate cytochrome b (cytb) gene. Nested PCR was subsequently used to amplify the small subunit (SSU) rRNA, actin, heat shock protein 70 (HSP70), and 60 kDa glycoprotein (gp60) genes for the analysis of Cryptosporidium species, genotypes, and subtypes in the fecal samples. The infection rate of Cryptosporidium spp. in wild rodents from the northern foothills of the Dabie Mountains was 21.3% (57/267). Seven species of wild rodents were identified, and the infection rates for Cryptosporidium spp. varied among host species. In particular, the infection rate was 21.4% (25/117) in Niviventer lotipes, 22.4% (22/98) in Apodemus agrarius, 17.2% (5/29) in Rattus nitidus, 22.2% (4/18) in Apodemus draco, and 33.3% (1/3) in Rattus tanezumi. The identification results indicated the presence of five Cryptosporidium species: Cryptosporidium apodemi (n = 12), C. ubiquitum (n = 11), C. viatorum (n = 7), C. ratti (n = 2), and C. occultus (n = 2). Moreover, two novel genotypes were identified: Cryptosporidium sp. rat genotype VI (n = 8) and Cryptosporidium sp. rat genotype VII (n = 15). Notably, a new subtype of C. viatorum designated as XVgA4 was discovered. This study revealed the prevalence of Cryptosporidium spp. in wild rodents in the northern foothills of the Dabie Mountains and identified two novel Cryptosporidium genotypes, along with a new subtype, C. viatorum-XVgA4. The findings highlight the genetic diversity of Cryptosporidium spp., underscoring the increased risk of Cryptosporidium spp. transmission posed by local wild rodents population. It suggests that host-specific factors should be considered in epidemiological surveillance and control strategies of Cryptosporidium spp., which is of great significance for the prevention and control of Cryptosporidiosis.

With the development of Internet of Things (IOT) technology, V2X vehicle-road collaboration platform is rapidly applied to intelligent traffic control and road condition service. However, the existing platforms have the problems of inaccurate collection and release of traffic incidents, which can not solve the situation of a large number of secondary accidents and traffic congestion. To meet this demand, a road traffic incident collection and reporting device based on LTE communication is designed. As a roadside intelligent device of LTE-V2X vehicle infrastructure cooperative platform, it realizes the real-time and fast collection and reporting of traffic incidents, and sends them out in real time through V2X internet of vehicle service platform to help drivers adjust their driving routes and avoid congestion points. To help the traffic management department locate the incident quickly and deal with it effectively, so as to eliminate the negative impact of the incident on traffic operation in the shortest time, and further improve the road operation safety and smoothness index and operation efficiency.

The study provides phenotypic and molecular analyses of the antibiotic resistance in lactic acid bacteria (LAB) from fermented foods in Xi'an, China. LAB strains (n = 84) belonging to 16 species of Lactobacillus (n = 73), and Streptococcus thermophilus (n = 11) were isolated and identified by sequencing their 16S rRNA gene. All strains were susceptible to ampicillin, bacitracin, and cefsulodin, and intrinsically resistant to nalidixic acid, kanamycin, and vancomycin (except L. bulgaricus, L. acidophilus, and S. thermophilus, which were susceptible to vancomycin). Some strains had acquired resistance for penicillin (n = 2), erythromycin (n = 9), clindamycin (n = 5), and tetracycline (n = 14), while resistance to gentamycin, ciprofloxacin, streptomycin, and chloramphenicol was species dependant. Minimum inhibitory concentrations presented in this study will help to review microbiological breakpoints for some of the species of Lactobacillus. The erm(B) gene was detected from two strains of each of L. fermentum and L. vaginalis, and one strain of each of L. plantarum, L. salivarius, L. acidophilus, L. animalis, and S. thermophilus. The tet genes were identified from 12 strains of lactobacilli from traditional foods. This is the first time, the authors identified tet(S) gene from L. brevis and L. kefiri. The erm(B) gene from L. fermentum NWL24 and L. salivarius NWL33, and tet(M) gene from L. plantarum NWL22 and L. brevis NWL59 were successfully transferred to Enterococcus faecalis 181 by filter mating. It was concluded that acquired antibiotic resistance is well dispersed in fermented food products in Xi'an, China and its transferability to other genera should be monitored closely.

Zoonoses are increasingly recognized as an important burden on global public health in the 21st century. High-resolution, long-term field studies are critical for assessing both the baseline and future risk scenarios in a world of rapid changes. We have used a three-decade-long field study on hantavirus, a rodent-borne zoonotic pathogen distributed worldwide, coupled with epidemiological data from an endemic area of China, and show that the shift in the ecological dynamics of Hantaan virus was closely linked to environmental fluctuations at the human-wildlife interface. We reveal that environmental forcing, especially rainfall and resource availability, exert important cascading effects on intra-annual variability in the wildlife reservoir dynamics, leading to epidemics that shift between stable and chaotic regimes. Our models demonstrate that bimodal seasonal epidemics result from a powerful seasonality in transmission, generated from interlocking cycles of agricultural phenology and rodent behavior driven by the rainy seasons.

The obesity epidemic represents a critical public health issue worldwide, as it is a vital risk factor for many diseases, including type 2 diabetes (T2D) and cardiovascular disease. Obesity is a complex disease involving excessive fat accumulation. Proper adipose tissue accumulation and function are highly transcriptional and regulated by many genes. Recent studies have discovered that post-transcriptional regulation, mainly mediated by RNA-binding proteins (RBPs), also plays a crucial role. In the lifetime of RNA, it is bound by various RBPs that determine every step of RNA metabolism, from RNA processing to alternative splicing, nucleus export, rate of translation, and finally decay. In humans, it is predicted that RBPs account for more than 10% of proteins based on the presence of RNA-binding domains. However, only very few RBPs have been studied in adipose tissue. The primary aim of this paper is to provide an overview of RBPs in adipogenesis and adipose function. Specifically, the following best-characterized RBPs will be discussed, including HuR, PSPC1, Sam68, RBM4, Ybx1, Ybx2, IGF2BP2, and KSRP. Characterization of these proteins will increase our understanding of the regulatory mechanisms of RBPs in adipogenesis and provide clues for the etiology and pathology of adipose-tissue-related diseases.

From April to November 2016, two outbreaks of epidemic keratoconjunctivitis (EKC) occurred successively at primary and middle schools in the Tibet Autonomous Region of China, and a total of 197 clinically diagnosed cases were reported. Real-time PCR analyses confirmed that human adenovirus (HAdV) infection was related to these outbreaks. Further studies involving sequence determination and phylogenetic analysis based on the penton base, hexon, and fiber genes indicated that human adenovirus type 8 (HAdV-8), belonging to species D, was responsible for the outbreaks. This is the first report of a HAdV-8 associated EKC outbreak in mainland of China, and the results of this study are expected to provide support for future research into HAdV-8 in China.