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"Mao, Ji‐Wei"
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MicroRNA‐372 enhances radiosensitivity while inhibiting cell invasion and metastasis in nasopharyngeal carcinoma through activating the PBK‐dependent p53 signaling pathway
Nasopharyngeal carcinoma (NPC) is a common cancer found in the nasopharynx, which plagues countless NPC patients. MicroRNA‐372 (miR‐372) has been reported to be involved in various tumors. Here, we explored the important role of miR‐372 in radiosensitivity, invasion, and metastasis of NPC. Microarray analysis was conducted to search the NPC‐related differentially expressed genes (DEGs) and predict the miRs regulating PBK, which suggested that miR‐372 could influence the development of NPC via PBK and the p53 signaling pathway. Importantly, miR‐372 was observed to target PBK, thus down‐regulating its expression. Then, NPC 5‐8F and C666‐1 cells were selected, and treated with ionization radiation and alteration of miR‐372 and PBK expression to explore the functional role of miR‐372 in NPC. The expression of miR‐372, PBK, Bcl‐2, p53, and Bax as well as the extent of Akt phosphorylation were measured. In addition, cell colony formation, cell cycle, proliferation, apoptosis, migration, and invasion were detected. At last, tumor growth and the effect of miR‐372 on radiosensitivity of NPC were evaluated. Besides, over‐expressed miR‐372 down‐regulated Bcl‐2 and PBK expression and the extent of Akt phosphorylation while up‐regulated the expression of p53 and Bax. Additionally, miR‐372 over‐expression and radiotherapy inhibited cell clone formation, proliferation, tumor growth, migration, invasion, and cell cycle entry, but promoted cell apoptosis. However, the restoration of PBK in NPC cells expressing miR‐372 reversed the anti‐tumor effect of miR‐372 and activation of the p53 signaling pathway. In conclusion, the study shows that up‐regulated miR‐372 promotes radiosensitivity by activating the p53 signaling pathway via inhibition of PBK.
In conclusion, the study shows that up‐regulated miR‐372 promotes radiosensitivity by activating the p53 signaling pathway via inhibition of PBK.
Journal Article
Numerical Analysis of Tunneling Influence on Settlement of Existing Pile Foundations Based on Orthogonal Design
Because of the Limited urban space and the development of transport, underground space development, especially the metro construction, is vigorously developed in many cities. Subway passing through the existing building inevitably may cause the settlement and deformation of the buildings and their foundations. Therefore, research on the influence of tunneling on existing buildings has important significance. In order to study the influence of tunneling on settlement of existing pile foundations, soil elastic modulus, cohesion, friction angle, tunneling sizes, distance between tunnel opening boundaries and existing pile foundation etc. are discussed as analysis factors in this paper. The calculation schemes are designed based on orthogonal design, and then the influence of tunneling on existing buildings is researched according to the numerical calculation model which is established by the finite element software. Finally, the sensitivity of pile foundation settlement to various factors is analyzed according to the results of simulation analysis. we can draw the conclusions: the influence of tunneling on settlement of existing pile foundation is positively correlated with tunneling sizes, and is negatively correlated with soil parameters and distance between tunnel opening boundaries and existing pile foundations; The settlement of pile foundations can be effectively controlled by application of composite anchor isolation piles around the existing buildings piles, but with the increase of soil parameters and distance between tunnel opening boundaries and existing pile foundations, the strengthening effects are decreased; soil elastic modulus and excavation sizes are the key impact-factors of pile settlement within the distance of 5m between tunneling boundaries and existing pile foundations, which have extremely remarkable effect on the subsidence of pile foundations.
Journal Article
Spin mapping of intralayer antiferromagnetism and field-induced spin reorientation in monolayer CrTe2
Intrinsic antiferromagnetism in van der Waals (vdW) monolayer (ML) crystals enriches our understanding of two-dimensional (2D) magnetic orders and presents several advantages over ferromagnetism in spintronic applications. However, studies of 2D intrinsic antiferromagnetism are sparse, owing to the lack of net magnetisation. Here, by combining spin-polarised scanning tunnelling microscopy and first-principles calculations, we investigate the magnetism of vdW ML CrTe
2
, which has been successfully grown through molecular-beam epitaxy. We observe a stable antiferromagnetic (AFM) order at the atomic scale in the ML crystal, whose bulk is ferromagnetic, and correlate its imaged zigzag spin texture with the atomic lattice structure. The AFM order exhibits an intriguing noncollinear spin reorientation under magnetic fields, consistent with its calculated moderate magnetic anisotropy. The findings of this study demonstrate the intricacy of 2D vdW magnetic materials and pave the way for their in-depth analysis.
In two dimensions magnetic order without magnetic anisotropy is forbidden, making 2D magnetic systems a rich playground for interesting physics. Here, Xian et al. fabricate monolayers of CrTe2, and demonstrate antiferromagnetic ordering, with spin reorientation at finite magnetic fields.
Journal Article
Exploring atomic defects in molybdenum disulphide monolayers
Defects usually play an important role in tailoring various properties of two-dimensional materials. Defects in two-dimensional monolayer molybdenum disulphide may be responsible for large variation of electric and optical properties. Here we present a comprehensive joint experiment–theory investigation of point defects in monolayer molybdenum disulphide prepared by mechanical exfoliation, physical and chemical vapour deposition. Defect species are systematically identified and their concentrations determined by aberration-corrected scanning transmission electron microscopy, and also studied by
ab-initio
calculation. Defect density up to 3.5 × 10
13
cm
−2
is found and the dominant category of defects changes from sulphur vacancy in mechanical exfoliation and chemical vapour deposition samples to molybdenum antisite in physical vapour deposition samples. Influence of defects on electronic structure and charge-carrier mobility are predicted by calculation and observed by electric transport measurement. In light of these results, the growth of ultra-high-quality monolayer molybdenum disulphide appears a primary task for the community pursuing high-performance electronic devices.
Imperfections can greatly alter a material’s properties. Here, the authors investigate the influence of point defects on the electronic structure, charge-carrier mobility and optical absorption of molybdenum disulphide prepared by mechanical exfoliation, physical and chemical vapour deposition.
Journal Article
Non-Destructive Testing of Alfalfa Seed Vigor Based on Multispectral Imaging Technology
Seed vigor is an important index to evaluate seed quality in plant species. How to evaluate seed vigor quickly and accurately has always been a serious problem in the seed research field. As a new physical testing method, multispectral technology has many advantages such as high sensitivity and accuracy, nondestructive and rapid application having advantageous prospects in seed quality evaluation. In this study, the morphological and spectral information of 19 wavelengths (365, 405, 430, 450, 470, 490, 515, 540, 570, 590, 630, 645, 660, 690, 780, 850, 880, 940, 970 nm) of alfalfa seeds with different level of maturity and different harvest periods (years), representing different vigor levels and age of seed, were collected by using multispectral imaging. Five multivariate analysis methods including principal component analysis (PCA), linear discriminant analysis (LDA), support vector machine (SVM), random forest (RF) and normalized canonical discriminant analysis (nCDA) were used to distinguish and predict their vigor. The results showed that LDA model had the best effect, with an average accuracy of 92.9% for seed samples of different maturity and 97.8% for seed samples of different harvest years, and the average sensitivity, specificity and precision of LDA model could reach more than 90%. The average accuracy of nCDA in identifying dead seeds with no vigor reached 93.3%. In identifying the seeds with high vigor and predicting the germination percentage of alfalfa seeds, it could reach 95.7%. In summary, the use of Multispectral Imaging and multivariate analysis in this experiment can accurately evaluate and predict the seed vigor, seed viability and seed germination percentages of alfalfa, providing important technical methods and ideas for rapid non-destructive testing of seed quality.
Journal Article
The Response of Soil Respiration to Temperature and Humidity in the Thermokarst Depression Zone of the Headwater Wetlands of Qinghai Lake
As the climate warms, the thickening of the active layer of permafrost has led to permafrost melting and surface collapse, forming thermokarst landforms. These changes significantly impact regional vegetation, soil physicochemical properties, and hydrological processes, thereby exacerbating regional carbon cycling. This study analyzed the relationship between soil respiration rate (Rs), soil temperature (T), and volumetric water content (VWC) in the thermokarst depression zone of the headwater wetlands of Qinghai Lake, revealing their influence on these soil parameters. Results showed a significant positive correlation between soil temperature and Rs (p < 0.001), and a significant negative correlation between VWC and Rs (p < 0.001). The inhibitory effect of VWC on Rs in the thermokarst depression zone was stronger than under natural conditions (p < 0.05). Single-factor models indicated that the temperature-driven model had higher explanatory power for Rs variation in both the thermokarst depression zone (R2 = 0.509) and under natural conditions (R2 = 0.414), while the humidity-driven model had lower explanatory power. Dual-factor models further improved explanatory power, slightly more so in the thermokarst depression zone. This indicates that temperature and humidity jointly drive Rs. Additionally, during the daytime, temperature had a more significant impact on Rs under natural conditions, while increased VWC inhibited Rs. At night, the positive correlation between Rs and temperature in the thermokarst depression zone increased significantly. The temperature sensitivity (Q10) values of Rs were 3.32 and 1.80 for the thermokarst depression zone and natural conditions, respectively, indicating higher sensitivity to temperature changes at night in the thermokarst depression zone. This study highlights the complexity of soil respiration responses to temperature and humidity in the thermokarst depression zone of Qinghai Lake’s headwater wetlands, contributing to understanding carbon cycling in wetland ecosystems and predicting wetland carbon emissions under climate change.
Journal Article
Enhanced recovery after surgery (ERAS) relieves psychological stress in patients with osteoporotic vertebral compression fracture undergoing percutaneous kyphoplasty: an observational retrospective cohort study
Study design
This is an observational retrospective cohort study.
Objective
The purpose of this study is to investigate the incidence rate of depression and anxiety and the changes in patients treated with percutaneous kyphoplasty (PKP) following ERAS protocol.
Summary of background data
The incidence of depression and anxiety is not uncommon in patients with osteoporotic vertebral compression fracture (OVCF), which affects the prognosis of surgery. Enhanced recovery after surgery (ERAS) protocols can improve the perioperative stress response of patients.
Materials and methods
Patients were treated conventionally in 2019 as the control group (CG) (n = 281), and patients were treated according to the ERAS protocol in 2020 as the intervention group (IG) (n = 251). All patients were evaluated for depression and anxiety using Patient Health Questionnaire-9 (PHQ-9) and Generalized Anxiety Disorder-7 at admission, postoperative 1 week, 1 month and 3, 6, 12 months.
Results
The degree of depression statistically decreased in the IG at follow-up periods (
p
< 0.001), and the degree of anxiety statistically decreased at 1 week (
p
< 0.001), 1 month (
p
< 0.001), 3 months (
p
= 0.017). Patients in the IG could soothe depression and anxiety disorders faster than patients in the CG and maintain psychological stability at the follow-up periods. The percentage of moderate or above depression in the IG was statistically fewer than in the CG at follow-up periods (
p
< 0.01). The odds ratio (OR) was respectively 0.410, 0.357, 0.294, 0.333, 0.327 from 1 week to 12 months. While the percentage of patients with moderate or above anxiety significantly decreased in the IG at 1 week (
p
< 0.001), OR = 0.528, 1 month (
p
= 0.037), OR = 0.309 and 12 months (
p
= 0.040), OR = 0.554, no differences between 3 months (
p
= 0.187) and 6 months (
p
= 0.133).
Conclusion
PKP following ERAS protocol to treat patients with OVCF had a better effect on relieving postoperative anxiety and depression than following conventional protocol.
Journal Article
Metagenomic Analysis of the Rhizosphere Microbiome of Poa alpigena in the Qinghai Lake Basin Grasslands
Poa alpigena Lindm is a dominant forage grass in the temperate grasslands of the Qinghai Lake Basin, commonly used for grassland restoration. Soil microorganisms are crucial in material cycling within terrestrial ecosystems. This study aimed to investigate the effects of P. alpigena on the microbial community composition and structure in rhizosphere and non-rhizosphere soils in the Qingbaya grassland area. Using high-throughput sequencing, we identified microbial gene pools and compared microbial diversity. Metagenomic analysis showed that non-rhizosphere soil contained 35.42–36.64% known microbial sequences, with bacteria making up 79.25% of the microbiota. Alpha diversity analysis indicated significantly higher microbial richness and diversity in non-rhizosphere soil, influenced by electrical conductivity, total carbon, and total nitrogen content. LEfSe analysis revealed that Alphaproteobacteria and Betaproteobacteria were major differential taxa in rhizosphere and non-rhizosphere soils, respectively. Key metabolic pathways in rhizosphere microorganisms were related to AMPK signaling, secondary metabolite biosynthesis, and starch metabolism, while non-rhizosphere microorganisms were involved in aromatic compound degradation, purine metabolism, and microbial metabolism in diverse environments. The enrichment of microbial taxa and functional pathways related to methane oxidation in rhizosphere soil suggests a potential role of P. alpigena in shaping microbial processes linked to greenhouse gas regulation, although direct evidence of methane flux changes was not assessed. Similarly, the presence of aromatic compound degradation pathways in non-rhizosphere soil indicates microbial potential for processing such compounds, but no direct measurements of specific contaminants were performed.
Journal Article
A single-photon source based on topological bulk cavity
Topological photonics offers the potential to develop quantum light sources with inherent robustness against structural disorders. To date, topologically protected edge or corner states have been investigated for this purpose. Here, for the first time, we exploit a topological bulk state with vertical directionality to enhance the light emission from a single semiconductor quantum dot (QD). An irregular ‘Q’-shaped cavity is applied for establishing topological robustness. We experimentally demonstrate a 1.6-fold Purcell enhancement of single-photon emission in the topological bulk cavity, with tolerance to the emission wavelength or the positioning of the coupled QD. Simulations indicate that such a QD-cavity coupling system can retain a Purcell factor exceeding 1.6 under a broad spectral detuning range of 8.6 nm or a coverage area of 2.5 μm
2
. Furthermore, the optimized cavity structure integrated with a reflector predicts a high single-photon extraction efficiency up to 92%. Our results offer a novel approach to develop topologically protected quantum light sources with high extraction efficiency and robust QD-cavity interaction against irregular cavity boundaries.
We demonstrate a single-photon source based on topological bulk cavity, achieving high extraction efficiency and robust QD-cavity interaction against irregular cavity boundaries.
Journal Article