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"Marquardt, Meg"
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E-sports competitions
\"Explores the world of E-Sports competitions. Topics include locating and entering electronic game competitions, how they are structured, and rules competitors are expected to follow. Includes glossary, index, and bibliography for further reading\"-- Provided by publisher.
Book
Real-Time Imaging and Quantification of Amyloid-β Peptide Aggregates by Novel Quantum-Dot Nanoprobes
Protein aggregation plays a major role in the pathogenesis of neurodegenerative disorders, such as Alzheimer's disease. However, direct real-time imaging of protein aggregation, including oligomerization and fibrillization, has never been achieved. Here we demonstrate the preparation of fluorescent semiconductor nanocrystal (quantum dot; QD)-labeled amyloid-beta peptide (QDAbeta) and its advanced applications.
The QDAbeta construct retained Abeta oligomer-forming ability, and the sizes of these oligomers could be estimated from the relative fluorescence intensities of the imaged spots. Both QDAbeta coaggregation with intact Abeta42 and insertion into fibrils were detected by fluorescence microscopy. The coaggregation process was observed by real-time 3D imaging using slit-scanning confocal microscopy, which showed a typical sigmoid curve with 1.5 h in the lag-time and 12 h until saturation. Inhibition of coaggregation using an anti-Abeta antibody can be observed as 3D images on a microscopic scale. Microglia ingested monomeric QDAbeta more significantly than oligomeric QDAbeta, and the ingested QDAbeta was mainly accumulated in the lysosome.
These data demonstrate that QDAbeta is a novel nanoprobe for studying Abeta oligomerization and fibrillization in multiple modalities and may be applicable for high-throughput drug screening systems.
Journal Article
Wolves on the hunt
\"How do wolves work together to hunt? How long do wolves go between meals? Discover the answers to these questions and learn other fascinating facts about wolves' habitat, physical characteristics, and behavior.\"-- Provided by publisher.
Book
Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging
Metabolism and mitochondrial dysfunction are known to be involved in many different disease states. We have employed two-photon fluorescence imaging of intrinsic mitochondrial reduced nicotinamide adenine dinucleotide (NADH) to quantify the metabolic state of several cultured cell lines, multicell tumor spheroids, and the intact mouse organ of Corti. Historically, fluorescence intensity has commonly been used as an indicator of the NADH concentration in cells and tissues. More recently, fluorescence lifetime imaging has revealed that changes in metabolism produce not only changes in fluorescence intensity, but also significant changes in the lifetimes and concentrations of free and enzyme-bound pools of NADH. Since NADH binding changes with metabolic state, this approach presents a new opportunity to track the cellular metabolic state.
Journal Article
The science of a nuclear plant explosion
Examines the reasons behind and effects of nuclear power plant accidents.
Book
Polar bears on the hunt
\"Polar bears may seem cute and cuddly, but they are actually fierce predators! Find out how polar bears catch their prey, what makes them such powerful predators, and how their habitat supports these mighty hunters.\"-- Provided by publisher.
Book