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The Jupiter Trojan asteroids are a key population for understanding the chemical and dynamical evolution of the Solar System. Surface compositions of Trojans, in turn, provide crucial information for reconstructing their histories. NASA’s Lucy mission will soon complete the first spacecraft reconnaissance of this population. This review summarizes the current state of knowledge of Trojan surface compositions and looks ahead to expected advances in that knowledge from Lucy . Surface compositions of Trojans remain uncertain due to a relative lack of diagnostic absorption features, though dedicated observations have begun to provide some clues to compositions. Trojans have uniformly low albedos, with a population average of ∼5.3%, and red spectral slopes at ultraviolet, visible, and near-infrared wavelengths. A bimodality of spectral slopes has been detected and confirmed across all these wavelengths, and the ratio of “less-red” to “red” Trojans increases with decreasing size. A broad absorption at ∼3.1 μm in some less-red Trojans may indicate the presence of N-H bearing material. Mid-infrared emissivity spectra reveal the presence of fine-grained anhydrous silicates on the surfaces. The meteorite collection contains no identifiable analogs to Trojan asteroids. Among small body populations, some Main Belt asteroids, comets, irregular satellites, and Centaurs provide reasonable spectral matches, supporting some genetic relationships among some members of these groups. The cause of the observed spectral properties remains uncertain, but recent suggestions include a combination of volatile ice sublimation and space weathering or a combination of impact gardening and space weathering. The Lucy mission will provide detailed compositional analysis of (3548) Eurybates, (15094) Polymele, (11351) Leucus, (21900) Orus, and (617) Patroclus-Menoetius, a suite of targets that sample the diversity among the Trojan population along several dimensions. With these flybys, the Lucy mission is poised to resolve many of the outstanding questions regarding Trojan surface compositions, thereby revealing how the Trojans formed and evolved and providing a clearer view of Solar System history.

Gonadal hormones act throughout the brain , and neuropsychiatric disorders vary in symptom severity over the reproductive cycle, pregnancy, and perimenopause . Yet how hormones influence cognitive processes is unclear. Exogenous 17 -estradiol modulates dopamine signaling in the nucleus accumbens core (NAcc) , which instantiates reward prediction errors (RPEs) for reinforcement learning . Here we show that endogenous 17 -estradiol enhances RPEs and sensitivity to previous rewards by reducing dopamine reuptake proteins in the NAcc. Rats performed a task with different reward states; they adjusted how quickly they initiated trials across states, balancing effort against expected rewards. NAcc dopamine reflected RPEs that predicted and causally influenced initiation times. Elevated endogenous 17 -estradiol increased sensitivity to reward states by enhancing dopaminergic RPEs in the NAcc. Proteomics revealed reduced dopamine transporter expression. Finally, knockdown of midbrain estrogen receptors suppressed reinforcement learning. 17 -estradiol therefore controls RPEs via dopamine reuptake, mechanistically revealing how hormones influence neural dynamics for motivation and learning.

Primitive asteroids with low albedos and red slopes in the visible and near infrared (VNIR) are found in both the Main Belt and the Jupiter Trojan clouds. In order to determine whether the VNIR spectral similarities of primitive Main Belt asteroids and Jupiter Trojans are reflective of a true compositional similarity, we compare the mid-infrared silicate emission features of Main Belt and Jupiter Trojan asteroids. Using archival data from the Spitzer Space Telescope's IRS spectrograph and observations from the Stratospheric Observatory for Infrared Astronomy's (SOFIA) FORCAST instrument, we analyze the 5-40 micron spectra of thirteen primitive Main Belt asteroids and compare them to those of Jupiter Trojans in the literature. We find that while many primitive asteroids in the Main Belt resemble their Trojan counterparts with strong spectral signatures of olivine-rich high-porosity silicate regoliths, we identify (368) Haidea as a spectrally distinctive asteroid that lacks strong evidence of olivine in its MIR spectrum. Differences in silicate compositions among D-type asteroids imply a diversity of origins for primitive asteroids.

We present observations obtained with the Near Infrared Spectrograph on JWST of the five Jupiter Trojans that will be visited by the Lucy spacecraft -- the Patroclus-Menoetius binary, Eurybates, Orus, Leucus, and Polymele. The measured 1.7-5.3 \\(\\mu\\)m reflectance spectra, which provide increased wavelength coverage, spatial resolution, and signal-to-noise ratio over previous ground-based spectroscopy, reveal several distinct absorption features. We detect a broad OH band centered at 3 \\(\\mu\\)m that is most prominent on the less-red objects Eurybates, Patroclus-Menoetius, and Polymele. An additional absorption feature at 3.3-3.6 \\(\\mu\\)m, indicative of aliphatic organics, is systematically deeper on the red objects Orus and Leucus. The collisional fragment Eurybates is unique in displaying an absorption band at 4.25 \\(\\mu\\)m that we attribute to bound or trapped CO\\(_2\\). Comparisons with other solar system small bodies reveal broad similarities in the 2.7-3.6 \\(\\mu\\)m bands with analogous features on Centaurs, Kuiper belt objects (KBOs), and the active asteroid 238P. In the context of recent solar system evolution models, which posit that the Trojans initially formed in the outer solar system, the significant attenuation of the 2.7-3.6 \\(\\mu\\)m absorption features on Trojans relative to KBOs may be the result of secondary thermal processing of the Trojans' surfaces at the higher temperatures of the Jupiter region. The CO\\(_2\\) band manifested on the surface of Eurybates suggests that CO\\(_2\\) may be a major constituent in the bulk composition of Trojans, but resides in the subsurface or deeper interior and is largely obscured by refractory material that formed from the thermophysical processes that were activated during their inward migration.

Molecular networking has become a key method to visualize and annotate the chemical space in non-targeted mass spectrometry data. We present feature-based molecular networking (FBMN) as an analysis method in the Global Natural Products Social Molecular Networking (GNPS) infrastructure that builds on chromatographic feature detection and alignment tools. FBMN enables quantitative analysis and resolution of isomers, including from ion mobility spectrometry. Feature-based molecular networking allows the generation of molecular networks for mass spectrometry data that can recognize isomers, incorporate relative quantification and integrate ion mobility data.

Microplastics are widely dispersed throughout the marine environment. An understanding of the distribution and accumulation of this form of pollution is crucial for gauging environmental risk. Presented here is the first record of plastic contamination, in the 5 mm–250 μm size range, of Irish continental shelf sediments. Sixty-two microplastics were recovered from 10 of 11 stations using box cores. 97% of recovered microplastics were found to reside shallower than 2.5 cm sediment depth, with the area of highest microplastic concentration being the water-sediment interface and top 0.5 cm of sediments (66%). Microplastics were not found deeper than 3.5 ± 0.5 cm. These findings demonstrate that microplastic contamination is ubiquitous within superficial sediments and bottom water along the western Irish continental shelf. Results highlight that cores need to be at least 4–5 cm deep to quantify the standing stock of microplastics within marine sediments. All recovered microplastics were classified as secondary microplastics as they appear to be remnants of larger items; fibres being the principal form of microplastic pollution (85%), followed by broken fragments (15%). The range of polymer types, colours and physical forms recovered suggests a variety of sources. Further research is needed to understand the mechanisms influencing microplastic transport, deposition, resuspension and subsequent interactions with biota.

Passively grasping an unseen artificial finger induces ownership over this finger and an illusory coming together of one’s index fingers: a grasp illusion. Here we determine how interoceptive ability and attending to the upper limbs influence this illusion. Participants passively grasped an unseen artificial finger with their left index finger and thumb for 3 min while their right index finger, located 12 cm below, was lightly clamped. Experiment 1 (n = 30) investigated whether the strength of the grasp illusion (perceived index finger spacing and perceived ownership) is related to a person’s level of interoceptive accuracy (modified heartbeat counting task) and sensibility ( Noticing subscale of the Multidimensional Assessment of Interoceptive Awareness). Experiment 2 (n = 30) investigated the effect of providing verbal or tactile cues to guide participants’ attention to their upper limbs. On their own, neither interoceptive accuracy and sensibility or verbal and tactile cueing had an effect on the grasp illusion. However, verbal cueing increased the strength of the grasp illusion in individuals with lower interoceptive ability. Across the observed range of interoceptive accuracy and sensibility, verbal cueing decreased perceived index spacing by 5.6 cm [1.91 to 9.38] (mean [95%CI]), and perceived ownership by ∼3 points on a 7-point Likert scale (slope -0.93 [-1.72 to -0.15]). Thus, attending to the upper limbs via verbal cues increases the strength of the grasp illusion in a way that is inversely proportional to a person’s level of interoceptive accuracy and sensibility.

The gut microbiota has recently been proposed as a novel component in the regulation of host homeostasis and immunity. We have assessed for the first time the role of the gut microbiota in a mouse model of leukemia (transplantation of BaF3 cells containing ectopic expression of Bcr-Abl), characterized at the final stage by a loss of fat mass, muscle atrophy, anorexia and inflammation. The gut microbial 16S rDNA analysis, using PCR-Denaturating Gradient Gel Electrophoresis and quantitative PCR, reveals a dysbiosis and a selective modulation of Lactobacillus spp. (decrease of L. reuteri and L. johnsonii/gasseri in favor of L. murinus/animalis) in the BaF3 mice compared to the controls. The restoration of Lactobacillus species by oral supplementation with L. reuteri 100-23 and L. gasseri 311476 reduced the expression of atrophy markers (Atrogin-1, MuRF1, LC3, Cathepsin L) in the gastrocnemius and in the tibialis, a phenomenon correlated with a decrease of inflammatory cytokines (interleukin-6, monocyte chemoattractant protein-1, interleukin-4, granulocyte colony-stimulating factor, quantified by multiplex immuno-assay). These positive effects are strain- and/or species-specific since L. acidophilus NCFM supplementation does not impact on muscle atrophy markers and systemic inflammation. Altogether, these results suggest that the gut microbiota could constitute a novel therapeutic target in the management of leukemia-associated inflammation and related disorders in the muscle.

We examine in detail the AP-2 adaptin complex from the malaria parasite Plasmodium falciparum . In most studied organisms, AP-2 is involved in bringing material into the cell from outside, a process called endocytosis. Previous work shows that changes to the μ subunit of AP-2 can contribute to drug resistance. Our experiments show that AP-2 is essential for parasite development in blood but does not have any role in clathrin-mediated endocytosis. This suggests that a specialized function for AP-2 has developed in malaria parasites, and this may be important for understanding its impact on drug resistance. The efficacy of current antimalarial drugs is threatened by reduced susceptibility of Plasmodium falciparum to artemisinin, associated with mutations in pfkelch13 . Another gene with variants known to modulate the response to artemisinin encodes the μ subunit of the AP-2 adaptin trafficking complex. To elucidate the cellular role of AP-2μ in P. falciparum , we performed a conditional gene knockout, which severely disrupted schizont organization and maturation, leading to mislocalization of key merozoite proteins. AP-2μ is thus essential for blood-stage replication. We generated transgenic P. falciparum parasites expressing hemagglutinin-tagged AP-2μ and examined cellular localization by fluorescence and electron microscopy. Together with mass spectrometry analysis of coimmunoprecipitating proteins, these studies identified AP-2μ-interacting partners, including other AP-2 subunits, the K10 kelch-domain protein, and PfEHD, an effector of endocytosis and lipid mobilization, but no evidence was found of interaction with clathrin, the expected coat protein for AP-2 vesicles. In reverse immunoprecipitation experiments with a clathrin nanobody, other heterotetrameric AP-complexes were shown to interact with clathrin, but AP-2 complex subunits were absent. IMPORTANCE We examine in detail the AP-2 adaptin complex from the malaria parasite Plasmodium falciparum . In most studied organisms, AP-2 is involved in bringing material into the cell from outside, a process called endocytosis. Previous work shows that changes to the μ subunit of AP-2 can contribute to drug resistance. Our experiments show that AP-2 is essential for parasite development in blood but does not have any role in clathrin-mediated endocytosis. This suggests that a specialized function for AP-2 has developed in malaria parasites, and this may be important for understanding its impact on drug resistance.

Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized 1 , 2 . Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii . The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3 , showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals. Generation of a library of 62,389 mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii enables screening for genes required for photosynthesis and the identification of 303 candidate genes.