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The atmosphere of Mars is enriched in heavy isotopes with respect to Earth as a result of the escape of the atmosphere to space over billions of years. Estimating this enrichment requires a rigorous understanding of all atmospheric processes that contribute to the evolution of isotopic ratios between the lower and upper atmosphere, where escape processes take place. We combine measurements of CO vertical profiles obtained by the Atmospheric Chemistry Suite on board the ExoMars Trace Gas Orbiter with the predictions of a photochemical model and find evidence of a process of photochemistry-induced fractionation that depletes the heavy isotopes of C and O in CO (δ13C = −160 ± 90‰ and δ18O = −20 ± 110‰). In the upper atmosphere, accounting for this process reduces the escape fractionation factor by ~25%, suggesting that less C has escaped from the atmosphere of Mars than previously thought. In the lower atmosphere, incorporation of this 13C-depleted CO fractionation into the surface could support the abiotic origin of recently found Martian organics.Measurements by the ExoMars Trace Gas Orbiter reveal a depletion of heavy CO isotopes in Mars’s atmosphere caused by photochemistry-induced fractionation. The observed depletion in heavy C has implications for our understanding of C escape to space and the formation of organic material on Mars’s surface.

As many as 2500 interdune lakes lie within the Nebraska Sand Hills, a 50,000 km stabilized sand sea. The few published data on cores from these lakes indicate they are typically underlain by less than two m of Holocene lacustrine sediments. However, three lakes in the southwestern Sand Hills, Swan, Blue, and Crescent, contain anomalously thick marsh (peat) and lacustrine (gyttja) sediments. Swan Lake basin contains as much as 8 m of peat, which was deposited between about 9000 and 3300 years ago. This peat is conformably overlain by as much as 10.5 m of gyttja. The sediment record in Blue lake, which is 3 km downgradient from Swan lake, dates back to only about 6000 years ago. Less than two m of peat, which was deposited from 6000 to 5000 years ago, is overlain by 12 m of gyttja deposited in the last 4300 years. Crescent Lake basin, one km downgradient from Blue Lake, has a similar sediment history except for a lack of known peat deposits. Recently, a 8-km long segment of a paleovalley was documented running beneath the three lakes and connecting to the head of Blue Creek Valley. Blockage of this paleovalley by dune sand during two arid intervals, one shortly before 10 500 yr BP and one in the mid-Holocene, has resulted in a 25 m rise in the regional water table. This made possible the deposition of organic-rich sediment in all three lakes. Although these lakes, especially Swan, would seem ideal places to look for a nearly complete record of Holocene climatic fluctuations, the paleoclimatic record is confounded by the effect dune dams have on the water table. In Swan Lake, the abrupt conversion from marsh to lacustrine deposition 3300 years ago does not simply record the change to a wetter regional climate; it reflects the complex local hydrologic changes surrounding the emplacement and sealing of dune dams, as well as regional climate.

As many as 2500 interdune lakes lie within the Nebraska Sand Hills, a 50 000 km stabilized sand sea. The few published data on cores from these lakes indicate they are typically underlain by less than two m of Holocene lacustrine sediments. However, three lakes in the southwestern Sand Hills, Swan, Blue, and Crescent, contain anomalously thick marsh (peat) and lacustrine (gyttja) sediments. Swan Lake basin contains as much as 8 m of peat, which was deposited between about 9000 and 3300 years ago. This peat is conformably overlain by as much as 10.5 m of gyttja. The sediment record in Blue lake, which is 3 km downgradient from Swan lake, dates back to only about 6000 years ago. Less than two m of peat, which was deposited from 6000 to 5000 years ago, is overlain by 12 m of gyttja deposited in the last 4300 years. Crescent Lake basin, one km downgradient from Blue Lake, has a similar sediment history except for a lack of known peat deposits. Recently, a 8-km long segment of a paleovalley was documented running beneath the three lakes and connecting to the head of Blue Creek Valley. Blockage of this paleovalley by dune sand during two arid intervals, one shortly before 10500 yr BP and one in the mid-Holocene, has resulted in a 25 m rise in the regional water table. This made possible the deposition of organic-rich sediment in all three lakes. Although these lakes, especially Swan, would seem ideal places to look for a nearly complete record of Holocene climatic fluctuations, the paleoclimatic record is confounded by the effect dune dams have on the water table. In Swan Lake, the abrupt conversion from marsh to lacustrine deposition 3300 years ago does not simply record the change to a wetter regional climate; it reflects the complex local hydrologic changes surrounding the emplacement and sealing of dune dams, as well as regional climate.

In the ProtecT trial, over 1600 men with PSA-detected localized prostate cancer were assigned to active monitoring, prostatectomy, or radiotherapy. Although more patients assigned to active monitoring had disease progression, overall survival was similar in the three groups. The management of clinically localized prostate cancer that is detected on the basis of prostate-specific antigen (PSA) levels remains controversial. In the United States alone, an estimated 180,890 cases will be diagnosed in 2016, and 26,120 men will die from the disease. 1 The widespread use of PSA testing has resulted in a dramatic increase in the diagnosis and treatment of prostate cancer, but many men do not benefit from intervention because the disease is either indolent or disseminated at diagnosis. Prostate cancer often progresses slowly, and many men die of competing causes. In addition, interventions for prostate cancer can have . . .

In men with prostate cancer on PSA screening, radical treatments led to half the incidence of metastasis and local progression as active monitoring without affecting disease-specific or overall survival.

A phenotype of Escherichia coli and Klebsiella pneumoniae, resistant to piperacillin/tazobactam (TZP) but susceptible to carbapenems and 3rd generation cephalosporins, has emerged. The resistance mechanism associated with this phenotype has been identified as hyperproduction of the β-lactamase TEM. However, the mechanism of hyperproduction due to gene amplification is not well understood. Here, we report a mechanism of gene amplification due to a translocatable unit (TU) excising from an IS26-flanked pseudo-compound transposon, PTn6762, which harbours bla . The TU re-inserts into the chromosome adjacent to IS26 and forms a tandem array of TUs, which increases the copy number of bla leading to TEM-1B hyperproduction and TZP resistance. Despite a significant increase in bla copy number, the TZP-resistant isolate does not incur a fitness cost compared to the TZP-susceptible ancestor. This mechanism of amplification of bla is an important consideration when using genomic data to predict susceptibility to TZP.

Plant biotechnology is rife with new advances in transformation and genome engineering techniques. A common requirement for delivery and coordinated expression in plant cells, however, places the design and assembly of transformation constructs at a crucial juncture as desired reagent suites grow more complex. Modular cloning principles have simplified some aspects of vector design, yet many important components remain unavailable or poorly adapted for rapid implementation in biotechnology research. Here, we describe a universal Golden Gate cloning toolkit for vector construction. The toolkit chassis is compatible with the widely accepted Phytobrick standard for genetic parts, and supports assembly of arbitrarily complex T‐DNAs through improved capacity, positional flexibility, and extensibility in comparison to extant kits. We also provision a substantial library of newly adapted Phytobricks, including regulatory elements for monocot and dicot gene expression, and coding sequences for genes of interest such as reporters, developmental regulators, and site‐specific recombinases. Finally, we use a series of dual‐luciferase assays to measure contributions to expression from promoters, terminators, and from cross‐cassette interactions attributable to enhancer elements in certain promoters. Taken together, these publicly available cloning resources can greatly accelerate the testing and deployment of new tools for plant engineering. Core ideas Phytobrick parts library for genome engineering includes developmental regulators, recombinases, and reporters Modular cloning toolkit simplifies assembly of complex constructs, especially binary vectors Benchmarking of cis‐regulatory elements for dicot and monocot gene expression helps guide construct design Plant housekeeping gene promoters show minimal enhancer activity in comparison to viral promoters

A phenotype of Escherichia coli and Klebsiella pneumoniae , resistant to piperacillin/tazobactam (TZP) but susceptible to carbapenems and 3rd generation cephalosporins, has emerged. The resistance mechanism associated with this phenotype has been identified as hyperproduction of the β-lactamase TEM. However, the mechanism of hyperproduction due to gene amplification is not well understood. Here, we report a mechanism of gene amplification due to a translocatable unit (TU) excising from an IS 26 -flanked pseudo-compound transposon, PTn 6762 , which harbours bla TEM-1B . The TU re-inserts into the chromosome adjacent to IS 26 and forms a tandem array of TUs, which increases the copy number of bla TEM-1B, leading to TEM-1B hyperproduction and TZP resistance. Despite a significant increase in bla TEM-1B copy number, the TZP-resistant isolate does not incur a fitness cost compared to the TZP-susceptible ancestor. This mechanism of amplification of bla TEM-1B is an important consideration when using genomic data to predict susceptibility to TZP. An E. coli and K. pneumoniae phenotype resistant to piperacillin/tazobactam has recently emerged. Here, the authors show that hyperproduction of the β-lactamase driving this resistance occurs due to excision and reinsertion of a translocatable unit containing bla TEM-1B , creating a tandem array.

Two nanometre gold nanoparticles (AuNPs), bearing sugar moieties and/or thiol-polyethylene glycol-amine (PEG-amine), were synthesised and evaluated for their in vitro toxicity and ability to radiosensitise cells with 220 kV and 6 MV X-rays, using four cell lines representing normal and cancerous skin and breast tissues. Acute 3 h exposure of cells to AuNPs, bearing PEG-amine only or a 50:50 ratio of alpha-galactose derivative and PEG-amine resulted in selective uptake and toxicity towards cancer cells at unprecedentedly low nanomolar concentrations. Chemotoxicity was prevented by co-administration of N-acetyl cysteine antioxidant, or partially prevented by the caspase inhibitor Z-VAD-FMK. In addition to their intrinsic cancer-selective chemotoxicity, these AuNPs acted as radiosensitisers in combination with 220 kV or 6 MV X-rays. The ability of AuNPs bearing simple ligands to act as cancer-selective chemoradiosensitisers at low concentrations is a novel discovery that holds great promise in developing low-cost cancer nanotherapeutics.