Explore the vast range of titles available.

Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m 3 industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17 th October – 14 th December). Optimisation of tubular PBR operations showed that improved productivities were obtained at a culture velocity of 0.65–1.35 m s −1 and a pH set-point for CO 2 injection of 8.0. Highest volumetric (0.08 ± 0.01 g L −1 d −1 ) and areal (20.3 ± 3.2 g m −2 d −1 ) biomass productivities were attained in the 100-m 3 PBR compared to those of the 35-m 3 PBR (0.05 ± 0.02 g L −1 d −1 and 13.5 ± 4.3 g m −2 d −1 , respectively). Lipid contents were similar in both PBRs (9–10% of ash free dry weight). CO 2 sequestration was followed in the 100-m 3 PBR, revealing a mean CO 2 mitigation efficiency of 65% and a biomass to carbon ratio of 1.80. Tetraselmis sp. CTP4 is thus a robust candidate for industrial-scale production with promising biomass productivities and photosynthetic efficiencies up to 3.5% of total solar irradiance.

Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35-and 100-m(3) industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17th October -14th December). Optimisation of tubular PBR operations showed that improved productivities were obtained at a culture velocity of 0.65-1.35 m s(-1) and a pH set-point for CO2 injection of 8.0. Highest volumetric (0.08 +/- 0.01 g L-1 d(-1)) and areal (20.3 +/- 3.2 g m(-2) d(-1)) biomass productivities were attained in the 100-m(3) PBR compared to those of the 35-m(3) PBR (0.05 +/- 0.02 g L-1 d(-1) and 13.5 +/- 4.3 g m(-2) d(-1), respectively). Lipid contents were similar in both PBRs (9-10% of ash free dry weight). CO2 sequestration was followed in the 100-m(3) PBR, revealing a mean CO2 mitigation efficiency of 65% and a biomass to carbon ratio of 1.80. Tetraselmis sp. CTP4 is thus a robust candidate for industrial-scale production with promising biomass productivities and photosynthetic efficiencies up to 3.5% of total solar irradiance.

High expectations are placed on microalgae as a sustainable source of valuable biomolecules. Robust methods to control microalgae cultivation processes are needed to enhance their efficiency and, thereafter, increase the profitability of microalgae-based products. To meet this need, a non-invasive monitoring method based on a hyperspectral imager was developed for laboratory scale and afterwards tested on industrial scale cultivations. In the laboratory experiments, reference data for microalgal biomass concentration was gathered to construct 1) a vegetation index-based linear regression model and 2) a one-dimensional convolutional neural network model to resolve microalgae biomass concentration from the spectral images. The two modelling approaches were compared. The mean absolute percentage error (MAPE) for the index-based model was 15–24%, with the standard deviation (SD) of 13-18 for the different species. MAPE for the convolutional neural network was 11–26% (SD = 10–22). Both models predicted the biomass well. The convolutional neural network could also classify the monocultures of green algae by species (accuracy of 97–99%). The index-based model was fast to construct and easy to interpret. The index-based monitoring was also tested in an industrial setup demonstrating a promising ability to retrieve microalgae-biomass-based signals in different cultivation systems.

Industrial production of novel microalgal isolates is key to improving the current portfolio of available strains that are able to grow in large-scale production systems for different biotechnological applications, including carbon mitigation. In this context, Tetraselmis sp. CTP4 was successfully scaled up from an agar plate to 35- and 100-m industrial scale tubular photobioreactors (PBR). Growth was performed semi-continuously for 60 days in the autumn-winter season (17 October - 14 December). Optimisation of tubular PBR operations showed that improved productivities were obtained at a culture velocity of 0.65-1.35 m s and a pH set-point for CO injection of 8.0. Highest volumetric (0.08 ± 0.01 g L d ) and areal (20.3 ± 3.2 g m d ) biomass productivities were attained in the 100-m PBR compared to those of the 35-m PBR (0.05 ± 0.02 g L d and 13.5 ± 4.3 g m d , respectively). Lipid contents were similar in both PBRs (9-10% of ash free dry weight). CO sequestration was followed in the 100-m PBR, revealing a mean CO mitigation efficiency of 65% and a biomass to carbon ratio of 1.80. Tetraselmis sp. CTP4 is thus a robust candidate for industrial-scale production with promising biomass productivities and photosynthetic efficiencies up to 3.5% of total solar irradiance.

A intervenção em âmbitos urbanos consolidados pressupõe o reconhecimento de um conjunto de acções anteriores sobrepostas num mesmo lugar.A partir do tema Construir no Construído, é objectivo compreender o papel da arquitectura na transformação de áreas sedimentadas que carreguem um grau de definição reconhecível e validade histórica mas consideradas como insuficiências na capacidade de constituir cidade.O trabalho investiga a hipótese da reactivação dos lugares baseada nas suas características intrínsecas, através da proposta de soluções programáticas e formais que tirem partido do vínculo natural com a estrutura urbana, por forma a operar dentro da complexidade temporal que caracteriza a experiência da cidade.Neste sentido, a arquitectura actua como sistema de relações; articulação que integra a significação cultural nos usos que propõe, viabilizando leituras que compatibilizem os temas da permanência e da mudança ao serviço da regeneração da cidade e espaço público.O caso prático de projecto consiste na ampliação da Biblioteca Municipal de Belém, actualmente sediada no corpo poente do Palácio do Marquês de Angeja, à Junqueira. A intervenção procura radicar-se nos valores essenciais da préexistência e é desenvolvida no sentido de incorporar os elementos existentes e o seu significado na narrativa do projecto.

One patient presented hyporexia, asthenia, adynamia, and jaundice two months after acute yellow fever (YF) onset; plus laboratory tests indicating hepatic cytolysis and a rebound of alanine and aspartate transaminases, and total and direct bilirubin levels. Laboratory tests discarded autoimmune hepatitis, inflammatory or metabolic liver disease, and new infections caused by hepatotropic agents. Anti-YFV IgM, IgG and neutralizing antibodies were detected in different times, but no viremia. A liver biopsy was collected three months after YF onset and tested positive for YFV antigens and wild-type YFV-RNA (364 RNA-copies/gram/liver). Transaminases and bilirubin levels remained elevated for five months, and the arresting of symptoms persisted for six months after the acute YF onset. Several serum chemokines, cytokines, and growth factors were measured. A similar immune response profile was observed in the earlier phases of the disease, followed by more pronounced changes in the later stages, when transaminases levels returned to normal. The results indicated viral persistence in the liver and continual liver cell damage three months after YF onset and reinforced the need for extended follow-ups of YF patients. Further studies to investigate the role of possible viral persistence and the immune response causing relapsing hepatitis following YF are also necessary.