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With sustainability within food systems becoming an increasingly important issue, several approaches that claim to assess the sustainability of farms, farming systems, and supply chains have been developed. Looking more closely at these sustainability impact assessment approaches, we discerned considerable differences between them in terms of scope, the level of assessment, and the precision of indicators used for impact assessment. Our aim was to classify and analyze a range of available sustainability impact assessment approaches with respect to scope and precision. From a total of 35 sustainability assessment approaches, we selected 6 for a detailed comparison. From our analysis, we concluded that there are 3 different types of trade-offs in these approaches: between different kinds of scope, between different indicators for precision and trade-offs, and between the scope and precision. Thus, one-size-fits-all solutions, with respect to tool selection, are rarely feasible. Furthermore, as indicator selection determines the assessment results, different and inconsistent indicators can lead to contradictory assessment results that may not be comparable. To overcome these shortcomings, sustainability impact assessments should include a precise definition of the notion of “sustainability” along with a description of the methodological approach and the indicator sets and should aim for harmonization of indicators and assumptions. Global initiatives such as the Sustainability Assessment in Food and Agriculture Systems (SAFA) Guidelines are a helpful step toward shedding light on the differences of these approaches and making the assessment results more comparable.

Diabetes mellitus is a metabolic disorder that affects more than 460 million people worldwide. Type 1 diabetes (T1D) is caused by autoimmune destruction of β-cells, whereas type 2 diabetes (T2D) is caused by a hostile metabolic environment that leads to β-cell exhaustion and dysfunction. Currently, first-line medications treat the symptomatic insulin resistance and hyperglycaemia, but do not prevent the progressive decline of β-cell mass and function. Thus, advanced therapies need to be developed that either protect or regenerate endogenous β-cell mass early in disease progression or replace lost β-cells with stem cell-derived β-like cells or engineered islet-like clusters. In this Review, we discuss the state of the art of stem cell differentiation and islet engineering, reflect on current and future challenges in the area and highlight the potential for cell replacement therapies, disease modelling and drug development using these cells. These efforts in stem cell and regenerative medicine will lay the foundations for future biomedical breakthroughs and potentially curative treatments for diabetes.Diabetes is a substantial and increasing health concern. In this Review, Lickert and colleagues discuss the progress made in developing insulin-producing islets using in vitro methods, including which aspects need to be improved in order to use these islets as transplants. Using these islets in laboratory settings could further our understanding of pancreatic function and the mechanisms underlying diabetes.

In recent years, more agricultural lands are been converted to photovoltaic (PV) power plants for better return on investment. However, prioritizing energy generation over food production poses a significant threat to the well-being of the rapidly growing global population. Agro-photovoltaics (APV) provide an opportunity to integrate crop production under PV panels. The objective of this study was to investigate the effect of APV system on microclimate, photosynthesis, and agronomic performance of mungbean in a tropical environment. Five mungbean genotypes, Tvr18, Tvr28, Tvr65, Tvr79 and Tvr83 were assessed under three APV micro environments, East-west facing PV (WPV), West-east facing PV (EPV), and no PV (NPV) in a split plot design with 5 replications. Results obtained showed significant reduction ( p  < 0.05) in photosynthetic active radiation (5–47%), leaf temperature (3–9%), and in the proportion of potentially harmful unregulated energy reaching the reaction centers (19–23%) under the PV (% reduction in WPV > EPV). Relative humidity, photochemical energy conversion, plant height, number of leaves, pods, and seeds were increased significantly ( p  < 0.05) underneath the EPV compared to NPV. Seed weight also increased non-significantly under EPV while flowering and podding behaviour, leaf area and stem diameter were comparable ( p  > 0.05) in NPV and EPV. We report for the first time that microclimate, growth, photochemistry and yield performances of mungbean were improved under APV system in a tropical environment. The improved performances of mungbean under EPV compared to WPV suggest that PV orientation is important and should not be overlooked in APV system designs.

Impact ejecta formation and emplacement is of great importance when it comes to understanding the process of impact cratering and consequences of impact events in general. Here we present a multidisciplinary investigation of a distal impact ejecta layer, the Blockhorizont, that occurs near Bernhardzell in eastern Switzerland. We provide unambiguous evidence that this layer is impact-related by confirming the presence of shocked quartz grains exhibiting multiple sets of planar deformation features. Average shock pressures recorded by the quartz grains are ~ 19 GPa for the investigated sample. U–Pb dating of zircon grains from bentonites in close stratigraphic context allows us to constrain the depositional age of the Blockhorizont to ~ 14.8 Ma. This age, in combination with geochemical and paleontological analysis of ejecta particles, is consistent with deposition of this material as distal impact ejecta from the Ries impact structure, located ~ 180 km away, in Germany. Our observations are important for constraining models of impact ejecta emplacement as ballistically and non-ballistically transported fragments, derived from vastly different depths in the pre-impact target, occur together within the ejecta layer. These observations make the Ries ejecta one of the most completely preserved ejecta deposit on Earth for an impact structure of that size.

Fire blight (FB), caused by Erwinia amylovora, is one of the most important pome fruit pathogens worldwide. To control this devastating disease, various chemical and biological treatments are commonly applied in Switzerland, but they fail to keep the infection at an acceptable level in years of heavy disease pressure. The Swiss authorities therefore currently allow the controlled use of the antibiotic streptomycin against FB in years that are predicted to have heavy infection periods, but only one treatment per season is permitted. Another strategy for controlling Erwinia is to breed resistant/tolerant apple cultivars. One way of accelerating the breeding process is to obtain resistant cultivars by inserting one or several major resistance genes, using genetic engineering. To date, no study summarizing the impact of different FB control measures on the environment and on human health has been performed. This study consequently aims to compare different disease-control measures (biological control, chemical control, control by antibiotics and by resistant/tolerant apple cultivars obtained through conventional or molecular breeding) applied against E. amylovora, considering different protection goals (protection of human health, environment, agricultural diversity and economic interest), with special emphasis on biosafety aspects. Information on each FB control measure in relation to the specified protection goal was assessed by literature searches and by interviews with experts. Based on our results it can be concluded that the FB control measures currently applied in Switzerland are safe for consumers, workers and the environment. However, there are several gaps in our knowledge of the human health and environmental impacts analyzed: data are missing (1) on long term studies on the efficacy of most of the analyzed FB control measures; (2) on the safety of operators handling streptomycin; (3) on residue analyses of Equisetum plant extract, the copper and aluminum compounds used in apple production; and (4) on the effect of biological and chemical control measures on non-target fauna and flora. These gaps urgently need to be addressed in the near future.

Religion lebt in Bildern. Bilder stiften Gemeinschaft. Gleichzeitig ziehen Bilder Grenzen zwischen Menschen. In diesem Spannungsfeld erforscht die vorliegende Arbeit Kriterien eines angemessenen Umgangs mit religioser Bildlichkeit in interkulturellen und interreligiosen Lernsituationen. Diese interdisziplinare Forschungsarbeit im Schnittbereich von Religions- und Kunstpadagogik analysiert die Bedeutung verbildlichter Religion fur das interkulturelle und interreligiose Lernen zwischen Menschen christlicher und islamischer Pragung. Dazu untersucht der Autor zentrale Verbildlichungen des Christentums und des Islams vor dem Hintergrund imaginationstheoretischer und bildwissenschaftlicher Erkenntnisse und im Abgleich mit religionspadagogischen und kunstpadagogischen Bildumgangskonzepten. Darauf aufbauend entwickelt er Grundlagen einer interkulturellen und interreligiosen Bilddidaktik und legt dar, welchen Beitrag Religions- und Kunstpadagogik zu einem mundigen Umgang mit kulturellen Figurationen im Allgemeinen und mit religiosen Verbildlichungen im Speziellen leisten konnen.

Spatially resolved transcriptomics of tissue sections enables advances in fundamental and applied biomedical research. Here, we present Multiplexed Deterministic Barcoding in Tissue (xDBiT) to acquire spatially resolved transcriptomes of nine tissue sections in parallel. New microfluidic chips were developed to spatially encode mRNAs over a total tissue area of 1.17 cm 2 with a 50 µm resolution. Optimization of the biochemical protocol increased read and gene counts per spot by one order of magnitude compared to previous reports. Furthermore, the introduction of alignment markers allowed seamless registration of images and spatial transcriptomic spots. Together with technological advances, we provide an open-source computational pipeline to prepare raw sequencing data for downstream analysis. The functionality of xDBiT was demonstrated by acquiring 16 spatially resolved transcriptomic datasets from five different murine organs, including the cerebellum, liver, kidney, spleen, and heart. Factor analysis and deconvolution of spatial transcriptomes allowed for in-depth characterization of the murine kidney. Examining the spatially resolved transcriptome of tissue sections promises advances in biomedical research. Here, the authors present xDBiT, a versatile, microfluidics-based approach to cost-effectively measure the spatial transcriptome of multiple tissue sections in parallel.

Determining the structure of water adsorbed on solid surfaces is a notoriously difficult task and pushes the limits of experimental and theoretical techniques. Here, we follow the evolution of water agglomerates on Fe₃O₄(001); a complex mineral surface relevant in both modern technology and the natural environment. Strong OH–H₂O bonds drive the formation of partially dissociated water dimers at low coverage, but a surface reconstruction restricts the density of such species to one per unit cell. The dimers act as an anchor for further water molecules as the coverage increases, leading first to partially dissociated water trimers, and then to a ring-like, hydrogen-bonded network that covers the entire surface. Unraveling this complexity requires the concerted application of several state-of-the-art methods. Quantitative temperature-programmed desorption (TPD) reveals the coverage of stable structures, monochromatic X-ray photoelectron spectroscopy (XPS) shows the extent of partial dissociation, and noncontact atomic force microscopy (AFM) using a CO-functionalized tip provides a direct view of the agglomerate structure. Together, these data provide a stringent test of the minimum-energy configurations determined via a van der Waals density functional theory (DFT)-based genetic search.

Oligonucleotide-conjugated antibodies have gained importance for their use in protein diagnostics. The possibility to transfer the readout signal from the protein to the DNA level with an oligonucleotide-conjugated antibody increased the sensitivity of protein assays by orders of magnitude and enabled new multiplexing strategies. A bottleneck in the generation of larger oligonucleotide-conjugated antibody panels is the low conjugation yield between antibodies and oligonucleotides, as well as the lack of product purification methods. In this study, we combined a non-site-directed antibody conjugation technique using copper-free click chemistry with ion-exchange chromatography to obtain purified single and double oligonucleotide-conjugated antibodies. We optimized the click conjugation reaction of antibodies with oligonucleotides by evaluating crosslinker, reaction temperature, duration, oligonucleotide length, and secondary structure. As a result, we were able to achieve conjugation yields of 30% at a starting quantity as low as tens of nanograms of antibody, which makes the approach applicable for a wide variety of protein analytical assays. In contrast to previous non-site-directed conjugation methods, we also optimized the conjugation reaction for antibody specificity, confirmed by testing with knockout cell lines. The advantages of using single or double oligonucleotide-conjugated antibodies in regards to signal noise reduction are shown within immunofluorescence, proximity ligation assays, and single cell CITE-seq experiments.

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot de novo assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic in vivo processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by Escherichia coli. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date.