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Tomato is a popularly consumed vegetable fruit and suffers from huge losses due to its high perishability. Calcium chloride (CaCl2∙2H2O) application has been shown to be an important method that can extend the shelf life of tomato fruits. The current study aimed to determine the effect of pre-harvest treatments of CaCl2 on the growth, yield, quality, and shelf-life performance of tomato varieties. Four tomato varieties known as MT-1, MT-3, 303, and 105 were sprayed with the CaCl2 solutions (0.0%, 1.0%, 1.5%, and 2.0%, w/v) after seven days of fruit initiation and were sprayed every week until the first harvest. Spraying with 2% of CaCl2 showed an improvement in controlling physiological disorders such as blossom end rot (BER), weight loss, declined disease incidence, and disease severity. An increase in fruit quality was also observed as the treated fruit could maintain its shelf life up to 20 days at ambient conditions. At the highest doses of CaCl2 (2%), it increased the total ascorbic acid (AA), lycopene content, total phenolic content (TPC), and antioxidant activity (DPPH) in comparison to lower doses and control tomatoes. MT-3 responded well to higher doses of CaCl2 (2%) among the four tomato varieties and performed better in growth, yield, disease, insect infestation, quality, and shelf-life performance compared to the other varieties and CaCl2 treatment combination.

Drying and storage are the common postharvest issues in seed production. Normally, seeds are harvested at physiological maturity when the moisture is higher than desired for safe storage. This study aims to evaluate the use of common drying methods and suggest a suitable storage temperature for the lablab bean seed. Pods at 30 days after anthesis are harvested and the seeds subjected to drying using sun, shade, oven, and drying beads to obtain target moisture contents of 14%, 12%, and 10%. Dried seeds are then stored at two storage temperatures; ambient (27.7–34.2 °C; 74 ± 5% relative humidity) and refrigerated (2.0–5.0 °C; 25 ± 5% relative humidity) for six months. Both shade and drying beads provided good results. However, drying beads gave the highest germination percentage (≥70%) and the shortest drying duration to achieve safe moisture content of 10%, along with the economic benefit from the repeated usage. The beads can be reused indefinitely by recharging in an oven between uses, thus, indicating the potential adoption by farmers. Two commonly used methods (sun and oven) have been proven to be unsuitable as they increase dead seeds due to heating damage. It is further explained that during six months of storage in the ambient temperature, declines were recorded in antioxidant enzyme activities, germination performance, and seedling growth. Therefore, lablab bean seeds are best stored refrigerated (≤5 °C) in which the germination and defense mechanism are maintained, and it is highly recommended to the farmers and seed producers as it is easily accessible, cost-saving, and sustainable.

Phyllanthus niruri (P. niruri) or Dukung Anak is a herbal plant in the Phyllanthaceae family that has been used traditionally to treat various ailments such as diabetes, jaundice, flu and cough. P. niruri contains numerous medicinal benefits such as anti-tumor and anti-carcinogenic properties and a remedy for hepatitis B viral infection. Due to its beneficial properties, P. niruri is overharvested and wild plants become scarce. This study was conducted to develop an appropriate in vitro culture protocol for the mass production of P. niruri. An aseptic culture of P. niruri was established followed by multiplication of explants using different types of basal medium and its strength and plant growth regulators manipulation. This study also established the induction of in vitro rooting utilizing various types and concentrations of auxin. Treatment of Clorox® with 30% concentration showed the lowest percentage (%) of contamination, 4.44% in P. niruri culture. Nodal segments of P. niruri were successfully induced in full-strength of Murashige and Skoog (MS) basal media with 2.33 number of shoots, 3.11 cm length of shoot and 27.91 number of leaves. In addition, explants in full-strength MS media without any additional cytokinin were recorded as the optimum results for all parameters including the number of shoots (5.0 shoots), the length of shoots (3.68 cm) and the number of leaves (27.33 leaves). Treatment of 2.5 µM indole-3-butyric acid (IBA) showed the highest number of roots (17.92 roots) and root length (1.29 cm). Rooted explants were transferred for acclimatization, and the plantlet showed over 80% of survival rate. In conclusion, plantlets of P. niruri were successfully induced and multiplied via in vitro culture, which could be a step closer to its commercialization.

The failure to promptly eliminate excessive reactive oxygen species (ROS) leads to the oxidation of biological macromolecules such as proteins, which is a key factor in chilling injury (CI) in harvested fruits and vegetables. Methionine sulfoxide reductase (Msr) is a class of redox proteins that reduce methionine sulfoxide (MetSO) in oxidized proteins back to methionine (Met), thereby restoring protein function. In recent years, the role of Msr in protecting fruits and vegetables from CI has attracted increasing research interest. This review summarizes the classification, distribution, and subcellular localization of Msr in plants and examines its roles and regulatory mechanisms in mitigating CI. The discussion focuses on postharvest CI, ROS dynamics, and Msr-related regulatory pathways. This review provides insights into improving plant quality and enhancing cold resistance through genetic engineering.

Nine morphologically distinct kenaf genotypes were hybridized to produce 36 hybrids following a half diallel mating design. The combining ability and gene action of 15 yield and yield components were assessed in hybrids and their parents across two environments. Except for the mid diameter and plant height traits, there were highly significant differences (p ≤ 0.01) between the environments and the interaction of genotype and environment. Additive gene effects were considerable for the inheritance of these traits, and the expression of these additive genes was heavily influenced by the environment. Significant differences were found for all studied traits for GCA except top diameter and SCA except plant height and top diameter, implying the presence of both additive and non-additive gene action for the inheritance of the concerned characters. For all features except top diameter and number of nodes, the magnitude of GCA variation was significantly higher than that of SCA variance, indicating the additive gene's predominance. The parental lines P 1 , P 3 and P 4 were outstanding general combiners for fiber yield and yield-related parameters. Considering combining ability and genetic analysis study, the crosses P 1  × P 4 , P 1  × P 9 , P 2  × P 3 , P 2  × P 5 , P 4  × P 6 , P 4  × P 7 , P 4  × P 9 , P 5  × P 8 , and P 7  × P 9 were found promising for their heterotic response to higher fiber yield, stick yield, seed yield and could be for future improvement in kenaf breeding programmes.

It is crucial to assess genetically superior parents when developing novel hybrids. This experiment was conducted to find out the diversity of 27 Capsicum annuum mutant lines derived from two varieties.To achieve the objective, 23 morpho-physiological and yield traits were recorded through two planting seasons. Highly significant differences (p < 0.01) were recorded among the studied traits. There was a strong to moderately positive phenotypic association between yield and all other morphological traits except first bifurcation length, stem diameter, pedicle length, flowering date, and maturity date. A higher Genotypic Coefficient of Variation (GCV) and Phenotypic Coefficient of Variation (PCV), combined with moderate to high heritability and high hereditary progress, have been found in the number of fruits per plant, fruit yield per plant, and number of seeds per fruit. High heritability was found in yield characteristics, vis-à-visnumber of seeds per fruit, number of fruits per plant, and indicated high genetic advance. The studied genotypes were divided into six groups after the cluster analysis. Based on the correlation matrix of 23 quantitative characteristics, principal component analysis revealed that the percentage of variation for PC1 and PC2 is 28%and 19%, respectively, andPC1 represents the largest percentage of the overall total variation. The calculated genetic distance also explains the potential of heterosis breeding. The revealed findings might be helpful for breeders to target quantitative characters and the parental lines of C. annuum during the execution of their future breeding programmes for developing high-yielding and climate-resilient chilli varieties.

Chilli (Capsicum annuum L.) is an herbaceous crop and plays an important role as common spices and vegetables. Pepper (Capsicum spp.) is one of the most cost-effective and agricultural vegetables in the world. The most significant characteristics of peppers, as spices and in various pharmacological uses, are pungency and oleoresin (color). Cabsicum annuum L. is widely used as a medicinal herb and in the Mediterranean diet (at the present, C. annuum var. acuminatum Fingerh. and Capsicum frutescens L. are considered synonyms of C. annuum L.). Capsicum annuum includes a wide range of carotenoids including capsanthin, capsorubin, beta-carotene, cryptoxanthin, lutein, fanthophyl, and xanthophyll, and capsaicinoid. However, it remains limited in production due to the lack of development in varieties especially under severe climatic circumstances such as drought, high temperature, or salt. Some reports were provided through distinct traditional approaches for genetic improvement. A combination of traditional and molecular breeding, especially breeding for heterosis, might be a good option for developing a novel genotype for ecologically adversely affected niche adaption. This review summarizes the current chilli breeding approaches with their drawbacks and highlights some recent classical efforts for the improvement of the crop. This would be the milestone for the breeders in the planning of a successful chilli breeding program to combat the adverse ecological condition. Thus, the information gathered in this article might be considered as the cornerstone of Chilli breeders at their ongoing and sustainable future programs as well.

Kenaf (Hibiscus cannabinus L.) is a natural fibre crop that can be used for a variety of purposes and has various applications in industry. Despite this, its potential has not been fully exploited because of low yields and a narrow genetic base, limiting hybrids’ development. Based on this background, eight kenaf mutants and one commercial cultivar were selected and crossed in a half-diallel for general and specific combining abilities (GCA and SCA) to get the desired results done in this investigation. The 36 hybrid offspring and their parental lines were tested in the field over two environments. Diallel results based on Griffing B method 2 indicated significant differences for all characters studied except for GCA in top diameter and plant height and top diameter SCA, indicating the existence of both additive and nonadditive gene actions for the inheritance of the traits. The amplitude of GCA variation was much higher than that of SCA variation for all parameters except top diameter and node number, showing the additive gene’s prevalence and the likelihood of genetic advancement through selection. In both conditions, Hayman and Jinks graphical studies demonstrated that partial dominance controlled various fibre yield component parameters such as plant height, middle diameter, stick weight, and fibre weight. On the other hand, fibre yield and the majority of physical features indicated either dominance or overdominance gene action. Plant height, base diameter, core diameter, middle diameter, fresh stem weight, and stick weight all strongly positively correlated with fibre yield. These traits also had a higher proportion of additive effects, a moderate narrow-sense heritability, and a higher baker ratio, indicating successful indirect selection for fibre yield. The parents P1, P3, and P4 had the most dominant alleles for most of the features, while the parents P2, P7, and P9 had the most recessive alleles. The hybrids P1×P4, P1×P9, P2×P3, P2×P5, P4×P6, P4×P7, P4×P9, P5×P8, and P7×P9 outperformed the parents in terms of heterotic responses and showed that they have a lot of genetic potential for kenaf enhancement in tropical climates.

Eleven morphologically diverse cultivated eggplant accessions were used for hybridization following half diallel mating design to obtain 55 hybrids. Evaluation of hybrids along with the parents was conducted over two locations followed by randomised complete block design with three replications to study gene action and combining ability of 15 morphological and biochemical traits. The analysis of variance indicated highly significant differences among the environments and interaction of genotype and environment, except for fruit length to width ratio. Additive gene effects were significant for the inheritance of these traits and expression of these additive genes were greatly affected by environments. The general combining ability (GCA) was greater than their respective specific combining ability (SCA) for all traits except for fruit yield per plant. High values of GCA and SCA effects for characters of interest were dispersed among different genotypes. From this study it was observed that the best parental line was BT15 based on days to first flowering, total number of fruits per plant, total soluble solids and total phenol content. Besides, the parent BM5 showed good general combining ability effects for fruit yield per plant, fruit length and fruit length to width ratio and the parent BB1 performed good general combining ability for fruit diameter, fruit girth and fruit weight. Besides, other parents showed the best performance for only one trait. On the other hand, the hybrid BT6 × BT15 was reported bearing early flowering with high total phenol content and the hybrid BM9 × BB26 has high fruit yield with high soluble solids. Besides, the hybrid BM9 × BB1 has a high fruit diameter and fruit weight. All other hybrids except for these three (BT6 × BT15, BM9 × BB26 and BM9 × BB1) were shown the best performance for only one trait. Hence, based on the desired trait, the hybrid can be selected for future use after large scale evaluation.

Main conclusion The study uncovers how Agastache rugosa coordinates carbon fixation, redox balance, and ATP homeostasis via distinct metabolic strategies optimized for different light and nutrient conditions. This study explores the metabolic adaptations of Agastache rugosa (Fisch. & C.A.Mey.) Kuntze in varying light and nutrient conditions, focusing on the coordination between photosynthetic and respiratory pathways. Plants were grown under two light levels (high light, 0% shade; low-light, 50% shade) and four nutrient treatments (NPK1, 40 mg kg −1 ; NPK2, 80 mg kg −1 ; NPK3, 120 mg kg −1 ; NPK4, 160 mg kg −1 ) and key metabolic parameters were analyzed. High-light plants had peak carbonic anhydrase activity (5.17 ± 0.26 U g −1 FW) at NPK2, optimizing carbon fixation and redox balance with 20.6% and 12.8% higher NADP + /NADPH and NAD + /NADH ratios, each. Low-light plants upregulated PEPC (+110%), and PEPCK (+34%) at NPK4, displaying enhanced anaplerotic carbon fixation. Despite lower respiratory activity, (NADH–UQ, −50%; COX, −46%), plants under low-light had tenfold higher ATP at NPK3 through reduced consumption. Principal component and hierarchical cluster analyses (> 60% similarity) revealed distinct metabolic strategies between light treatments. Strong correlations among photosynthetic, respiratory, and redox parameters (r > 0.7, P  < 0.001) indicated metabolic integration via shared regulatory networks. Our findings reveal the metabolic plasticity of A. rugosa , offering insights into plant adaptation with implications for cultivation. Moreover, multivariate analyses unveiled complex regulatory networks coordinating energy metabolism, highlighting the metabolic reprogramming employed by A. rugosa to maintain energetic and redox balance under dynamic environmental conditions.