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The biological manufacturing of zinc oxide nanoparticles (ZnO-NPs) using renewable sources is safe, harmless, and compatible with the environment. The capacity of Hypnea pannosa to synthesize ZnO-NPs was investigated in this work. Ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and zeta potential analysis were employed to characterize the ZnO-NPs. The created ZnO-NPs showed antimicrobial activity against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Acinetobacter baumannii, Candida albicans, and Candida auris. ZnO-NPs showed an MIC of 12.5 µg/mL against S. aureus, E. faecalis, K. pneumoniae, C. albicans, and C. auris, but it had a 25 µg/mL against Acinetobacter baumannii. ZnO-NPs’ ability to scavenge free radicals was assessed using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) technique with IC50 of 36.2 µg/mL. Anti-inflammatory activity of ZnO-NPs compared to indomethacin at 1000 µg/mL was investigated, where the membrane’s maximum stabilizer was 93.3%. ZnO-NPs demonstrated anticancer activity against PC3 and Caco2 cell lines with IC50 of 174.3 μg/mL and 83.3 μg/mL, respectively. Furthermore, ZnO-NPs demonstrated a range of anti-biofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, ZnO-NPs showed encouraging antiviral effect versus COX B4 as well as HSV1 with antiviral activities of 54.8% and 61.1%, respectively.

Natural constituents have been utilized to avoid humanity from various diseases, such as microbial infection and cancer, over several decades due to bioactive compounds. Myoporum serratum seeds extract (MSSE) was formulated via HPLC for flavonoid and phenolic analysis. Moreover, antimicrobial via well diffusion method, antioxidant via 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, anticancer activities against HepG-2 cells (human hepatocellular cancer cell line), and MCF-7 cells (human breast cancer cell line), and molecular docking of the main detected flavonoid and phenolic compounds with the cancer cells were performed. The phenolic acids, including cinnamic acid (12.75 µg/mL), salicylic acid (7.14 µg/mL), and ferulic (0.97 µg/mL), while luteolin represents the main detected flavonoid with a concentration of 10.74 µg/mL, followed by apegenin 8.87 µg/mL were identified in MSSE. Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Candida albicans were inhibited by MSSE with 24.33, 26.33, 20.67, and 18.33 mm of inhibition zone, respectively. MSSE exhibited a low inhibition zone of 12.67 mm against Escherichia coli while showing no inhibitory activity against Aspergillus fumigatus. The values of MIC ranged from 26.58 to 136.33 µg/mL for all tested microorganisms. MBC/MIC index and cidal properties were attributed to MSSE for all tested microorganisms except E. coli. MSSE demonstrated anti-biofilm 81.25 and 50.45% of S. aureus and E. coli, respectively. IC50 of the antioxidant activity of MSSE was 120.11 µg/mL. HepG-2 and MCF-7 cell proliferation were inhibited with IC50 140.77 ± 3.86 µg/mL and 184.04 µg/mL, respectively. Via Molecular docking study, luteolin and cinnamic acid have inhibitory action against HepG-2 and MCF-7 cells, supporting the tremendous anticancer of MSSE.

To prevent the rapidly increasing prevalence of bacterial resistance, it is crucial to discover new antibacterial agents. The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae has been associated with a higher mortality rate in gulf union countries and worldwide. Compared to physical and chemical approaches, green zinc oxide nanoparticle (ZnO-NP) synthesis is thought to be significantly safer and more ecofriendly. The present study used molecular dynamics (MD) to examine how ZnO-NPs interact with porin protein (GLO21), a target of β-lactam antibiotics, and then tested this interaction in vitro by determining the zone of inhibition (IZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC), as well as the alteration of KPC’s cell surface. The nanoparticles produced were characterized by UV-Vis spectroscopy, zetasizer, Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). In silico investigation was conducted using a variety of computational techniques, including Autodock Vina for protein and ligand docking and Desmond for MD simulation. The candidate ligands that interact with the GLO21 protein were biosynthesized ZnO-NPs, meropenem, imipenem, and cefepime. Analysis of MD revealed that the ZnO-NPs had the highest log P value (−9.1 kcal/mol), which indicates higher permeability through the bacterial surface, followed by cefepime (−7.9 kcal/mol), meropenem (−7.5 kcal/mol), and imipenem (−6.4 kcal/mol). All tested compounds and ZnO-NPs possess similar binding sites of porin proteins. An MD simulation study showed a stable system for ZnO-NPs and cefepime, as confirmed by RMSD and RMSF values during 100 ns trajectories. The test compounds were further inspected for their intersection with porin in terms of hydrophobic, hydrogen, and ionic levels. In addition, the stability of these bonds were measured by observing the protein–ligand contact within 100 ns trajectories. ZnO-NPs showed promising results for fighting KPC, represented in MIC (0.2 mg/mL), MBC (0.5 mg/mL), and ZI (24 mm diameter). To draw the conclusion that ZnO-NP is a potent antibacterial agent and in order to identify potent antibacterial drugs that do not harm human cells, further in vivo studies are required.

Nanotechnology is a rapidly developing field of research that studies materials having dimensions of less than 100 nanometers. It is applicable in many areas of life sciences and medicine including skin care and personal hygiene, as these materials are the essential components of various cosmetics and sunscreens. The aim of the present study was to synthesize Zinc oxide (ZnO) and Titanium dioxide (TiO2) nanoparticles (NPs) by using Calotropis procera (C. procera) leaf extract. Green synthesized NPs were characterized by UV spectroscopy, Fourier transform infrared (FTIR), X-ray diffraction (XRD), and Scanning Electron Microscopy (SEM) to investigate their structure, size, and physical properties. The antibacterial and synergistic effects of ZnO and TiO2 NPs along with antibiotics were also observed against bacterial isolates. The antioxidant activity of synthesized NPs was analyzed by their α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity. In vivo toxic effects of the synthesized NPs were evaluated in albino mice at different doses (100, 200, and 300 mg/kg body weight) of ZnO and TiO2 NPs administered orally for 7, 14, and 21 days. The antibacterial results showed that the zone of inhibition (ZOI) was increased in a concentration-dependent manner. Among the bacterial strains, Staphylococcus aureus showed the highest ZOI, i.e., 17 and 14 mm against ZnO and TiO2 NPs, respectively, while Escherichia coli showed the lowest ZOI, i.e., 12 and 10 mm, respectively. Therefore, ZnO NPs are potent antibacterial agents compared to TiO2 NPs. Both NPs showed synergistic effects with antibiotics (ciprofloxacin and imipenem). Moreover, the DPPH activity showed that ZnO and TiO2 NPs have significantly (p > 0.05) higher antioxidant activity, i.e., 53% and 58.7%, respectively, which indicated that TiO2 has good antioxidant potential compared to ZnO NPs. However, the histological changes after exposure to different doses of ZnO and TiO2 NPs showed toxicity-related changes in the structure of the kidney compared to the control group. The current study provided valuable information about the antibacterial, antioxidant, and toxicity impacts of green synthesized ZnO and TiO2 NPs, which can be influential in the further study of their eco-toxicological effects.

Silver nanoparticles (AgNPs) are valued for their antimicrobial properties, but conventional synthesis often involves toxic chemicals. Eco-friendly biosynthesis using silver-tolerant microbes from contaminated sites offers a sustainable alternative. This study biosynthesized and characterized AgNPs using a native Bacillus sp. from contaminated soil in the Jazan region, Saudi Arabia, and developed predictive models for optimizing synthesis and antimicrobial activity. AgNPs were synthesized under optimized conditions (1.0 mM AgNO3, 4.0 mL supernatant, pH 8, 85 °C). Characterization using UV–Vis, SEM, TEM, XRD, and FTIR assessed size, shape, structure, and chemistry. Gaussian and second models evaluated yield and inhibition zones based on AgNP concentration, microorganism type, and MIC. The AgNPs were spherical with diameters of 5–10 nm. The optimal nanoparticle yield occurs when the parameters are at their optimal values; C0 = 1.0 mM, V0 = 4.0 mL, pH0 = 8, T0 = 85 °C. XRD confirmed their crystalline nature, and FTIR showed biomolecular capping agents for stabilization. The Gaussian model accurately predicted synthesis efficiency, validated by 3D plots matching experimental data. The AgNPs showed strong antimicrobial activity against Gram-positive (Bacillus subtilis) (ATCC6051), Staphylococcus aureus (ATCC12600), Gram-negative bacteria Escherichia coli (ATCC11775) and fungi Candida albicans (ATCC10231); with E. coli having the lowest MIC (1.87 μg/mL). The inhibition zone model closely matched observed data. Biosynthesized AgNPs using silver-tolerant Bacillus sp. demonstrated potent antimicrobial effects and provide a green alternative to chemical synthesis. Integrating modeling optimizes biosynthesis and predicts biological performance, supporting future nanobiotechnology and antimicrobial applications.

Renewable energy can be harnessed from wastewater, whether from municipalities or industries, but this potential is often ignored. The world generates over 900 km3 of wastewater annually, which is typically treated through energy-consuming processes, despite its potential for energy production. Environmental pollution is a most important and serious issue for all and their adulterations to the aquatic system are very toxic in very low concentrations. Photocatalysis is a prominent approach to eliminating risky elements from the environment. The present study developed Zinc oxide (ZnO), Copper-doped Zinc oxide (CuZnO), and Cobalt-doped Zinc oxide (CoZnO) nanostructures (NSs) by facile hydrothermal route. The crystalline and structural stability of the synthesized nanostructures were evident from XRD and FESEM analysis. Metal, and oxygen bond and their interaction on the surfaces and their valency were explored from XPS spectra. Optical orientations and electron movements were revealed from UV-Visible analysis. After 100 min exposure time with 1 g of catalyst concentration 60%, 70%, and 89% of dye degraded, for dye concentration (5 mg/L to 50 mg/L), the huge variation observed (70% to 22%), (80% to 16%), (94% to 10%). The highest photodegradation rate (55%, 75%, 90%) was observed on pH~12 using ZnO, CoZnO, and CuZnO respectively. Photodegradation of methylene blue confirmed the largest surface area, rate of recombination, photo-excited charge carriers, photo-sensitivity range, and radical generations of ZnO, CuZnO, and CoZnO. The present study, therefore, suggested that CuZnO would be preferred to produce nanomaterials for industrial wastewater treatment like methylene.

The nanostructures synthesized using the green chemistry method have recently attracted the attention of scientists due to their significance in many scientific domains. This work provides an overview of the biosynthesis of zinc oxide (ZnO) nanosheets (NSs) using Phyllanthus emblica plant (PEP) extract. X-ray diffraction analysis (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) were used to analyze the synthesized ZnO-NSs. Evaluation of the antibacterial activity of biosynthesized ZnO-NSs was performed. ZnO-NSs exhibit effective antibacterial activity against Gram-positive (S. pyogenes and S. aureus) and Gram-negative (S. typhi and E. coli) bacterial strains. S. typhi is the most sensitive microbe towards ZnO-NSs and formed a 21 mm zone of inhibition (ZOI). ZnO-NSs are also tested as a photocatalyst in the degradation of methyl orange (MO) and rhodamine B (RB). The degradation rate of MO was 90%, and RB was 96% after being exposed to UV light for 120 min. The as-synthesized ZnO-NSs exhibited selective dye degradation and showed relatively better photocatalytic activity for positively charged (cationic) dyes. This work could lead to the fabrication of high-yield photocatalysts, which have the potential to degrade textile dyes from aqueous solution.

This study aimed to identify if Fusarium and Colletotrichum species are linked to coffee leaf wilt symptoms (Coffea arabica L.) gardens in Jazan region, Kingdom Saudi Arabia. The symptomatic wilted leaves and shoots were collected from Jazan Mountain Region Development Authority (JMRDA) farm in jabal Fifa. Samples of roots and leaves tissues were plated on Dox’ Agar medium and incubated for one week at 24oC. Two morphologically different fungus colonies grew on the medium. A PCR-based method was used for the molecular amplification and characterization of the fungi using a 18SrRNA specific primer. 1323 and 1501 bp PCR products were obtained by using the 1% agarose gel electrophoresis. The sequence analysis and genbank homology revealed that the present fungi were Fusarium oxysporum and Colletotrichum musae with 99 and 98% similarity, respectively. Both fungi sequences were submitted to the genebank under accession numbers OP010081 and OP010082, respectively. This is the first report of these two genera of fungi infecting the roots and leaves of coffee trees in Jazan Region of Saudi Arabia and suggests that other fungus species may play a significant role as diseases in other coffee-producing areas.

The eco‐friendly and cost‐effective biological synthesis of nanomaterials is rapidly gaining attention. This study synthesized silver nanoparticles (AgNPs) using an aqueous extract of Senna italica leaves and silver nitrate (AgNO 3 ). The synthesized AgNPs were characterized using UV‐Vis spectroscopy, Fourier‐transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X‐ray diffraction (XRD). UV‐Vis spectroscopy confirmed the formation of AgNPs, displaying a characteristic surface plasmon resonance peak at 445 nm. TEM and SEM analyses revealed spherical nanoparticles with sizes ranging from 12.7 to 24 nm. FTIR spectra identified bands at 1636 and 3496 cm −1 , corresponding to C=O and O‐H groups, indicating their role in stabilizing the nanoparticles. XRD analysis revealed diffraction planes at 111, 200, 220, and 311, consistent with the face‐centered cubic structure of silver. The AgNPs demonstrated significant antimicrobial activity against fungi and Gram‐negative and Gram‐positive bacteria, with Escherichia coli showing the highest sensitivity (MIC = 0.014 μ g/mL). SEM analysis of E. coli showed that untreated cells retained their normal morphology, whereas AgNP‐treated cells appeared shriveled and deformed. These results underscore the potential of Senna italica –derived AgNPs as effective antimicrobial agents. Future studies will be aimed at investigating the detailed mechanisms underlying the effects of AgNPs on bacterial cell structure and growth.

In this article, Persea americana aqueous peels extract was used to synthesize silver nanoparticles in a simple and environmentally friendly manner. The properties of synthesized silver nanoparticles (AV-AgNPs) were studied via several techniques, including UV–vis spectroscopy, DLS, TEM, EDX, and FTIR. The UV–Vis spectra analysis revealed that the highest absorption peak occurred at 428nm, providing strong evidence for forming AV-AgNPs. The TEM results indicate that the synthesized AV-AgNPs were uniformly dispersed, exhibiting a spherical shape with an average size of 24nm. EDX imaging also confirmed the presence of AgNPs. FTIR analysis shows P. americana's phenolic compounds and proteins significantly contribute to AV-AgNP synthesis and stabilization. In addition, the antimicrobial activity of AV-AgNPs was assessed against pathogenic bacteria commonly found in humans, exhibiting a moderate zone of inhibition against the selected pathogens. Additionally, AV-AgNPs were used in antioxidant studies with the robust antioxidant properties 2, 2 diphenyl-1-picrylhydrazyl (DPPH). In vitro studies have shown that AV-AgNPs can remarkably inhibit α-amylase in a dose-dependent manner, indicating their potential as antidiabetic agents. In silico research, it was revealed that naringenin derived from P. americana exhibited a strong binding to S. typhi and B. cereus (−8.7 and −7.5kcal/mol, respectively). At the same time, quercetin demonstrated a high binding affinity and energy to α-Amylase (−8.9kcal/mol). This study presents the first investigation into the biosynthesis of AgNPs utilizing the aqueous peel extract derived from P. americana with multiple uses, including antioxidant, antimicrobial, and antidiabetic activity.