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Heart failure (HF) is a progressive, multifaceted condition characterized by complex pathophysiological mechanisms, including chronic inflammation, which contributes significantly to disease onset and progression. Despite advancements in pharmacological therapies, such as angiotensin-converting enzyme inhibitors and β-blockers, HF remains associated with high morbidity and mortality. Inflammatory cytokines have emerged as potential biomarkers for HF, offering insights into disease mechanisms, risk stratification, and therapeutic responses. However, their clinical utility is hindered by analytical variability, pre-analytical challenges, and limited reproducibility across studies.This review explores the role of inflammatory cytokines in HF, evaluating their pathogenetic significance, analytical performance, and prognostic value. Biomarkers such as TNFα, IL-6, sTNFR1, and gp130 are assessed for their ability to enhance diagnostic precision and predict adverse outcomes. Furthermore, we examine the limitations of current cytokine assays, including sample stability, diurnal variation, and lack of standardization, which affect their transition into clinical practice. While natriuretic peptides and cardiac troponins remain the gold standard for HF biomarkers, multimarker approaches incorporating inflammatory cytokines may provide a more comprehensive understanding of HF pathophysiology.The findings underscore the need for robust, standardized methodologies and novel biomarkers beyond established pathways. These efforts will enhance HF management by improving risk stratification, guiding personalized treatment strategies, and advancing our understanding of inflammatory processes in HF progression.

Biochar, prepared from organic waste materials, can improve the quality of contaminated soil areas. Biochar can be used as an economic centerpiece over other available resources and can properly utilize large amounts of waste. Soil contaminated with cadmium (Cd) is a worldwide problem that poses potential agricultural and human health hazards. Moreover, Cd toxicity causes serious problems for sustainable food production, especially in food crops like barley. High cadmium concentration in soil is phytotoxic and decreases plant growth and ultimately yields. Biochar and ascorbic acid in ameliorating Cd stress are economically compatible and consistent approaches in agriculture. The present study aimed to evaluate biochar’s and foliar-applied ascorbic acid’s influence on some growth and biochemical characteristics of barley (Hordeum vulgare L.) to Cd stress. The soil was supplemented with biochar 2% w/w and 20 mg Cd kg−1. The foliar application of 30 mM ascorbic acid was done on plants. The results revealed that Cd stress decreased chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids. It also increased oxidative stress indicators, i.e., APX, COD, POD, flavonoids, anthocyanin, phenolics, and electrolyte leakage, in barley with Cd-contamination. A significant enhancement in root and shoot length, gas exchange attributes, and chlorophyll contents validated the effectiveness of Bio + Asa treatments over all other treatments under Cd contamination. In conclusion, the sole applications of biochar and Asa in Cd contamination are also effective, but Bio + Asa is a better amendment for Cd stress alleviation in barley plants.

L-glutaminase is an important anticancer agent that is used extensively worldwide by depriving cancer cells of L-glutamine. The marine bacterium, Halomonas meridian was isolated from the Red Sea and selected as the more active L-glutaminase-producing bacteria. L-glutaminase fermentation was optimized at 36 h, pH 8.0, 37 °C, and 3.0% NaCl, using glucose at 1.5% and soybean meal at 2%. The purified enzyme showed a specific activity of 36.08 U/mg, and the molecular weight was found to be 57 kDa by the SDS-PAGE analysis. The enzyme was highly active at pH 8.0 and 37 °C. The kinetics’ parameters of Km and Vmax were 12.2 × 10−6 M and 121.95 μmol/mL/min, respectively, which reflects a higher affinity for its substrate. The anticancer efficiency of the enzyme showed significant toxic activity toward colorectal adenocarcinoma cells; LS 174 T (IC50 7.0 μg/mL) and HCT 116 (IC50 13.2 μg/mL). A higher incidence of cell death was observed with early apoptosis in HCT 116 than in LS 174 T, whereas late apoptosis was observed in LS 174 T more than in HCT 116. Also, the L-glutaminase induction nuclear fragmentation in HCT 116 was more than that in the LS 174T cells. This is the first report on Halomonas meridiana as an L-glutaminase producer that is used as an anti-colorectal cancer agent.

Effective communication between hospital pharmacists and patients with disability is essential to ensure proper usage of prescribed medication, medication safety, and therapeutic outcomes. However, patients with hearing impairments encounter unique challenges that are often overlooked in pharmacy practice. There is limited evidence regarding how hospital pharmacists in Saudi Arabia address communication barriers with this patient group. To explore the experiences, challenges, and suggestions of hospital pharmacists in Saudi Arabia regarding communication with hearing-impaired patients in pharmacy settings. A qualitative descriptive study was conducted using an open-ended online survey distributed to hospital pharmacists across Saudi Arabia between April and May 2025. Twenty-six pharmacists responded to eight open-ended questions covering their experiences, training, communication barriers, and institutional support. Responses were analyzed thematically to identify key themes and insights. Six major themes resulted from the analysis: (1) lack of formal training and preparedness, (2) limited direct experience with hearing-impaired patients, (3) communication barriers including time constraints and lack of tools, (4) suggestions for communication aids such as tablets and visual boards, (5) institutional responsibilities for training and infrastructure, and (6) pharmacists' recommendations for inclusive service improvement. The findings highlight a critical gap in pharmacist training and system-level policies to support disability-inclusive communication in hospital pharmacy practice. Hospital pharmacists in Saudi Arabia reported limited preparedness and suggested various practical solutions, including written communication tools and visual aids. Findings indicate opportunities for structured training and institutional support to enhance inclusive communication.

This investigation explains the green synthesis, characterization and biocontrol potential of copper oxide nanoparticles (CuONPs) against second-stage juveniles (J2s) of root-knot nematode, Meloidogyne incognita infesting chickpea. Mono-disperse, spherical, pure CuONPs were synthesized from Jatropha curcas leaf with particle sizes ranging from 5 to 15 nm in diameter. Antagonistic activities of synthesized CuONPs were studied against Meloidogyne incognita. The highest mortality of J2s was found in the 200 ppm concentration of CuONPs at 24 h of exposure. The exact concentration also showed maximum inhibition of J2s hatching from egg masses after six days of exposure. It was worth noting that 25 ppm concentration was the least effective. The pot experiment showed that CuONPs significantly reduced the root infection caused by M. incognita and enhanced chickpea plants' growth and physiological attributes (Chlorophyll and carotenoid content). The results depicted when the concentration of CuONPs was increased, J2s mortality rate was also increased. We highlighted the antinematode influence of green synthesized CuONPs. Thus, it will offer an excellent eco-friendly strategy to optimize yield under pathogens attack and provide prospects of green synthesized-based nanoparticles development for pests control. Plants mediated CuONPs will also help in resolving the current toxicity concerns and future challenges in the agriculture.

Genetic polymorphism in long noncoding RNA (lncRNA) HOTAIR is linked with the risk and susceptibility of various cancers in humans. The mechanism involved in the development of CRC is not fully understood but single nucleotide polymorphisms (SNPs) can be used to predict its risk and prognosis. In the present case-control study, we investigated the relationship between HOTAIR (rs12826786, rs920778, and rs1899663) polymorphisms and CRC risk in the Saudi population by genotyping using a TaqMan genotyping assay in 144 CRC cases and 144 age- and sex-matched controls. We found a significant (p < 0.05) association between SNP rs920778 G > A and CRC risk, and a protective role of SNPs rs12826786 (C > T) and rs1899663 (C > A) was noticed. The homozygous mutant “AA” genotype at rs920778 (G > A) showed a significant correlation with the female sex and colon tumor site. The homozygous TT in SNP rs12816786 (C > T) showed a significant protective association in the male and homozygous AA of SNP rs1899663 (C > A) with colon tumor site. These results indicate that HOTAIR can be a powerful biomarker for predicting the risk of colorectal cancer in the Saudi population. The association between HOTAIR gene polymorphisms and the risk of CRC in the Saudi population was reported for the first time here.

Introduction: End-of-life medical care (ELMC) plans and do-not-resuscitate (DNR) decision-making are usually affected by multiple factors compared to other medical care decisions. ELMC and DNR policy implementation are still diversified and heterogeneous, especially in Saudi Arabia, because policymakers have adopted no guidelines. Thus, this study investigated physicians’ knowledge, attitude, and practice regarding ELMC and DNR. Methods: A cross-sectional study design was adopted. Three hundred physicians working at King Fahad Hospital of the University, Khobar, Saudi Arabia, were randomly selected and administered an anonymous self-administered questionnaire using the Likert scale. Data analysis was carried out using SPSS 23.0. Results: Of 300 distributed questionnaires, 264 (88%) were completed and analysed. Knowledge gaps and negative attitudes were observed, a quarter of the participants were opposed to issuing a DNR order, and 29.0% considered DNR as equal to euthanasia as they practice. The participants’ patient age and religious factors were the most critical factors in the ELMC plan and DNR decision. The physician’s level of acceptance regarding a set of ELMC interventions and DNR decisions showed heterogenicity and uncertainty among participants. Conclusions: The ELMC plan and DNR decision-making should be appropriately addressed in the medical residents’ training programs to bridge the knowledge gap and the physicians’ negative attitudes during their practice. Additionally, there is a need to update and unify the DNR policies at the national level, considering the patient’s right to be informed and involved actively during the decision process making. Finally, more prospective research is needed for the global standardization of ELMC.

Ventilator-associated pneumonia (VAP) is a common and serious healthcare-associated infection that occurs in mechanically ventilated patients in intensive care units (ICUs). It is associated with increased morbidity, mortality, length of hospital stay, and healthcare costs. Effective prevention and management of VAP require a multidisciplinary approach, with nurses and respiratory therapists playing central roles. This review examines the collaborative efforts of nurses and respiratory therapists in managing VAP, emphasizing evidence-based practices, prevention strategies, and the impact of teamwork on patient outcomes. It also explores challenges, innovations, and future directions for enhancing collaborative care.

Objective This study aimed to explore genetic variations associated with DNA repair mechanisms to enhance the management of both oral cancer (OC) and oral precancer (OPC). Methods A cohort of 380 patients diagnosed with OC and OPC, comprising 220 males and 160 females, was analyzed. Participants were categorized based on their tobacco-chewing habits, with corresponding control groups established. Key genetic markers investigated for polymorphisms included OGG1, APE1, and XRCC1. Results The XRCC1 Arg280H variant demonstrated significant associations with the susceptibility to both OC and OPC across various models. Further analyses, incorporating factors such as tobacco and alcohol consumption, unveiled a correlation between the XRCC1 Arg194Trp variant and an elevated risk of developing head and neck cancer. Stratified analyses also revealed an increased risk of OC or OPC based on the specific site of the cancer. Conclusion The study underscores the importance of XRCC1 polymorphisms, particularly XRCC1 Arg280H and XRCC1 Arg194Trp, within the genetic framework of OC and OPC. Understanding these genetic associations provides valuable insights for the potential development of targeted interventions aimed at individuals predisposed to these conditions.

Bio-based silica, lignocellulose, and activated carbon were simply produced via the recycling of Hassawi rice biomass waste of Al-Ahsa governorate in the eastern Saudi Arabia region using a fast chemical treatment procedure. Rice husk and rice straw wastes were collected, ground, and chemically treated with sodium hydroxide to extract silica/silicate from the dried plant tissues. The liquid extract is then treated with acid solutions in order to precipitate silica/silicate at neutral medium. Lowering the pH of the supernatant to 2 resulted in the precipitation of lignocellulose. Thermal treatment of the biomass residue under N2 gas stream resulted in activated carbon production. Separated products were dried/treated and characterized using several physical examination techniques, such as FT-IR, SEM/EDX, XRD, and Raman spectroscopy in order to study their structure and morphology. Silica and lignocelluloses products were then preliminarily used in the treatment of wastewaters and water-desalination processes.