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"Moorman, Gary"
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Wham! : teaching with graphic novels across the curriculum
\"This practical guide shows secondary school teachers how to incorporate graphic novels into content area instruction as a tool for meeting the needs of diverse learners and achieving the goals of the Common Core State Standards.\"-- Back cover.
Book
Digital Citizenship During a Global Pandemic: Moving Beyond Digital Literacy
In this commentary, the authors move beyond digital literacy and take up the question of what digital citizenship means and looks like in the context of the COVID‐19 pandemic. To engage with questions of ethical practice, the authors begin with the International Society for Technology in Education framework for digital citizenship. They expand on these standards to argue for an awareness of the ethical questions facing citizens online that are difficult to encompass as a set of skills or competencies. The authors then take these considerations into a set of practical steps for teachers to nurture participatory and social justice–oriented digital citizenship as part of the curriculum. The authors conclude by noting the digital divide and social inequities that have been highlighted by the current crisis.
Journal Article
Survival of Phytophthora alni, Phytophthora kernoviae, and Phytophthora ramorum in a simulated aquatic environment at different levels of pH
Abstract
Phytophthora ramorum, Phytophthora alni, and Phytophthora kernoviae present significant threats to biosecurity. As zoosporic oomycetes, these plant pathogens may spread through natural waterways and irrigation systems. However, survival of these pathogens in aquatic systems in response to water quality is not well understood. In this study, we investigated their zoospore survival at pH 3–11 in a 10% Hoagland's solution over a 14-day period. The results showed that all three pathogens were most stable at pH 7, although the populations declined overnight irrespective of pH. Extended survival of these species depended on the tolerance of pH of their germinants. Germinants of P. alni ssp. alni and P. ramorum were more basic tolerant (pH 5–11), while those of P. kernoviae were more acidic tolerant (pH 3–9). These tolerant germinants formed compact hyphae or secondary sporangia to allow longer survival of these pathogens. Long-term survival at a broad pH range suggests that these pathogens, especially P. ramorum, are adapted to an aquatic environment and pose a threat to new production areas through water dispersal.
Journal Article
Phytophthora irrigata, a new species isolated from irrigation reservoirs and rivers in Eastern United States of America
A new species of Phytophthora, previously referred to as the 'Dre I' taxon, is named Phytophthora irrigata. Isolates of P. irrigata morphologically and physiologically resemble Phytophthora drechsleri. They are heterothallic, produce nonpapillate sporangia, and grow well at 35 °C. The above two species differ significantly in uniformity of mycelium, presence of chlamydospores, DNA fingerprint, and sequences of multiple nuclear and mtDNA regions. Phytophthora irrigata produces smaller sporangia and a distinct DNA fingerprint. Sequence alignments in the ribosomal DNA internal transcribed spacer regions place Phytophthora fallax and Phytophthora captisoa as its closest relatives. The optimal temperature for culture growth is above 30 °C and the maximum temperature is 40 °C. This new species is abundant in irrigation reservoirs and natural waterways in Virginia and was also isolated in Pennsylvania.
Journal Article
Phytophthora aquimorbida sp. nov. and Phytophthora taxon 'aquatilis' recovered from irrigation reservoirs and a stream in Virginia, USA
Two distinct subgroups (L2 and A
−2
) were recovered from irrigation reservoirs and a stream in Virginia, USA. After molecular, morphological and physiological examinations, the L2 subgroup was named Phytophthora aquimorbida and the A
−2
designated as Phytophthora taxon 'aquatilis'. Both taxa are homothallic. P. aquimorbida is characterized by its noncaducous and nonpapillate sporangia, catenulate and radiating hyphal swellings and thick-walled plerotic oospores formed in globose oogonia mostly in the absence of an antheridium. P. taxon 'aquatilis' produces plerotic oospores in globose oogonia mostly with a paragynous antheridium. It has semi-papillate, caducous sporangia with variable pedicels, but it does not have hyphal swelling. Analyses of ITS, CO1, β-tubulin and NADH1 sequences revealed that P. aquimorbida is closely related to P. hydropathica, P. irrigata and P. parsiana, and P. taxon 'aquatilis' is related to P. multivesiculata. The optimum temperature for culture growth is 30 and 20 C for P. aquimorbida and P. taxon 'aquatilis' respectively. Both taxa were pathogenic to rhododendron plants and caused root discoloration, pale leaves, wilting, tip necrosis and dieback. Their plant biosecurity risk also is discussed.
Journal Article
Comparative Analysis of Water Quality between the Runoff Entrance and Middle of Recycling Irrigation Reservoirs
Recycling irrigation reservoirs (RIRs) are an emerging aquatic ecosystem of critical importance, for conserving and protecting increasingly scarce water resources. Here, we compare water quality between runoff entrance and middle of four RIRs in nurseries in Virginia (VA) and Maryland (MD). Surface water temperature (T) and oxidation-reduction potential (ORP) were lower in the middle than at the entrance, while the trend was opposite for dissolved oxygen (DO), pH and chlorophyll a (Chla). The magnitude of these differences between the entrance and middle decreased with increasing depth. These differences were magnified by water stratification from April to October. Minimum differences were observed for electrical conductivity (EC), total dissolved solids (TDS) and turbidity (TUR). Cluster analyses were performed on water quality difference data to evaluate whether the differences vary with respect to reservoirs. Two clusters were formed with one consisting primarily of VA reservoirs, and the other consisting mostly of MD reservoirs in both years. Water quality in the middle and at the entrance of RIRs was expected to vary greatly because of runoff inflow. The two-point water quality differences observed here, although statistically significant, are not large enough to cause significant impact on crop health and productivity for most water quality parameters except pH. Additional analysis of outlet data shows that the range and magnitude of water quality difference between the middle and the outlet are comparable to those between the middle and entrance of RIRs. These results indicate that monitoring at a single point is sufficient to obtain reliable water quality estimates for most water quality parameters in RIRs except pH. This is important when considering the cost of labor and equipment necessary for documenting water quality in agricultural production systems. However, additional pH measurements are still necessary to make practical water quality management decisions.
Journal Article
Identification and characterization of simple sequence repeat markers for Pythium aphanidermatum, P. cryptoirregulare, and P. irregulare and the potential use in Pythium population genetics
Six simple sequence repeat (SSR)-enriched genome libraries from Pythium aphanidermatum, P. irregulare, and P. cryptoirregulare were constructed to develop SSR markers. One hundred six SSR primer pairs for P. aphanidermatum, 73 for P. cryptoirregulare, and 82 for P. irregulare were initially identified. After examining primers, the most polymorphic and reproducible SSR markers were selected for each Pythium species; 14 in P. aphanidermatum, 21 in P. irregulare, and 22 in P. cryptoirregulare. Analysis of isolates from each Pythium species using SSR markers showed the high degree of gene diversity and polymorphic information content (PIC) value in the three species. The average number of alleles was 3.5-5.3 in the three Pythium species. Seven SSR loci from P. cryptoirregulare and P. irregualre showed the distinct genetic separations of P. irregualre complex isolates. SSR markers identified for the three Pythium target species were highly transferable to other closely related Pythium species. Cross-amplification was found in all SSR markers between P. cryptoirregulare and P. irregulare. SSR loci were successfully amplified by direct PCR from mycelia of P. aphanidermatum, P. cryptoirregulare, and P. irregulare. These newly developed SSR markers can be used for population genetic studies and monitoring the movement of isolates in crop production systems or in nature.
Journal Article
Genetic structure and distribution of Pythium aphanidermatum populations in Pennsylvania greenhouses based on analysis of AFLP and SSR markers
Pythium aphanidermatum is one of the most aggressive species in the genus and has a wide host range, but little is known about its population genetic structure. We tested 123 P. aphanidermatum isolates with six AFLP primer combinations and four SSR markers. The genetic diversity of P. aphanidermatum was 0.34 with AFLP and 0.55 with SSR markers. SSR genotypes totaled 3-8 for each locus, and a total of 14 SSR genotypes were found among all isolates. Three major genetic groups were identified with the combination of AFLP and SSR marker data. The genetic structure observed among P. aphanidermatum isolates was related to location and mefenoxam fungicide resistance instead of host. Four genotypes (PA1, PA2, PA5 and PA7) were found in the population from a commercial greenhouse, and the genetic diversity of a greenhouse population was similar to that found in the whole sample. The molecular tools for P. aphanidermatum isolates identified the possible gene flow within and among populations in Pennsylvania greenhouses.
Journal Article
Rethinking Plagiarism in the Digital Age
Plagiarism is a complex issue in need of reexamination. A common misconception is there is consensus on what constitute plagiarism, and general agreement that engaging in plagiarism and other forms of academic dishonesty is a major breech of ethics. There seems to be little concern for differentiating degrees of seriousness; the intentional copying of large amounts of text without acknowledgement is often viewed the same as failing to properly cite sources. Furthermore, instruction that addresses issues related to plagiarism is rare. In this article, the authors explore the issue in relation to the ever‐changing digital environment, and provide one specific example of explicit instruction that engages students in copying information from the Internet, rewriting or paraphrasing, then citing appropriately. Knowing students will utilize technology for writing and research, instruction should aim at demystifying the concept of plagiarism while at the same time improving students’ research and writing skills. FREE author podcast
Journal Article
Containment basin water quality fluctuation and implications for crop health management
Containment basins (CB) are an integral part of recycling irrigation systems that foster agricultural sustainability through water resource conservation. However, little is known regarding this aquatic ecosystem and the lack of water quality data has become an increasingly serious liability in crop health management. Here we report on four distinct seasonal and two diurnal patterns of change in water quality in the CBs. The four seasonal patterns are (a) periodic fluctuation in chlorophyll a, pH, and dissolved oxygen (DO), (b) oxidation-reduction potential (ORP) rises with decreasing DO, (c) tendency for increase in electrical conductivity, salinity, and total dissolved solids, and (d) weather-dependent changes in turbidity and temperature. The two diurnal patterns are (1) chlorophyll a, pH, DO, and temperature consistently peak between 16:00 and 17:00 hours and bottom out around 08:00 hours, and (2) ORP peaks in the morning and bottoms in the evening. Eight of the nine parameters excluding temperature were correlated; and algal blooms appear to be a major driving force for changes in the other seven parameters. These results underscore the importance of water quality monitoring in irrigation management and provide a framework for better understanding of pathogen aquatic ecology and how changes in water quality might be employed in a manner that suppresses plant pathogens and improves crop quality and productivity.
Journal Article