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This study deals with the anchorage of polyelectrolyte films onto titanium surfaces via a cathecol-based linker for biomedical applications. The following study uses a molecule functionalized with a catechol and a carboxylic acid: 3-(3,4-dihydroxyphenyl)propanoic acid. This molecule is anchored to the TiO(2) substrate via the catechol while the carboxylic acid reacts with polymers bearing amine groups. By providing a film anchorage of chemisorption type, it makes possible to deposit polyelectrolytes on the surface of titanium. Infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), contact angle and atomic force microscopy (AFM) measurements show that the different steps of grafting have been successfully performed. This method based on catechol anchorage of polyelectrolytes open a window towards large possibilities of clinical applications.

(2012) PEM Anchorage on Titanium Using Catechol Grafting. PLoS ONE 7(11): e50326. doi:10.1371/journal.pone.0050326 Citation: Marie H, Barrere A, Schoenstein F, Chavanne M-H, Grosgogeat B, Mora L (2013) Correction: PEM Anchorage on Titanium Using Catechol Grafting.

Understanding the mechanism of the bacterial cell adhesion to solid surfaces is of great medical and industrial importance. Bacterial adhesion to inert surfaces, such as a catheter, and other indwelling devices can form biofilm, consequently cause severe morbidity and often fatal infections. Initial bacterial adhesion to the material surfaces is a complicated process that is affected by various physicochemical properties of both bacterial cells and substratum surfaces. The surface properties of the cells were characterized by the sessile drop technique. Moreover, the interfacial free energy of Staphylococcus aureus adhesion to the supporting materials was determined. The results showed that S. aureus examined at different pH levels could be considered hydrophilic. We noted hat the electron-donor character of S. aureus was important at intermediate pH (pH 5, pH 7, and pH 9) and it decreased at both limits acidic and basic conditions. In addition, the adhesion of Staphylococcus aureus ATCC 25923 to the hydrophilic glass and hydrophobic indium tin oxide (ITO)-coated glass surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using atomic force microscopy (AFM) and image analysis was assessed with the Mathlab® program. The data analysis showed that cells (number of adhering cells to glass and ITO-coated glass surface) adhered strongly at acidic pH and weakly at alkaline pH. Also, S. aureus has the ability to attach to both hydrophobic and hydrophilic surfaces, but the adhesion was higher on hydrophobic surface.

It is important to analyze cell monolayer adherence for the development of biomedical devices of anti-thrombogenic vascular grafts. Endothelial cells must be firmly attached to the biomaterials when cells are seeded in order to create a natural lining. Polystyrene (PS) is presented as a reproducible implant model substrate for studying cell – material interactions. Polystyrene was deposited as a thin layer on a thiol functionalized gold electrode. Fibronectin (Fn), a protein promoting the cell monolayer adhesion was adsorbed on PS surface. The different steps of this multilayer assembly were characterized by Surface Plasmon Resonance (SPR) technique. A right shift of the SPR resonance angle θSPR was observed leading an increase from 65.5 deg in the case of gold electrode to 66.8 deg in the case where cell monolayer was cultured onto functionalized gold substrate. A shift in the SPR peak minimum intensity was detected in the SPR response of Au/Thiol/PS/Fn and Au/Thiol/PS/Fn/Cell multilayer assembly structures. This result is explained using Atomic Force Microscopy (AFM) images and according transverse profiles which indicate surface morphological modifications in term of thickness.

Atherosclerosis is a major cardiovascular disease. One of the side effects is restenosis. The aim of this work was to study the coating of stents by dextran derivates based polyelectrolyte’s multilayer (PEM) films in order to increase endothelialization of injured arterial wall after stent implantation. Films were composed with diethylaminoethyl dextran (DEAE) as polycation and dextran sulphate (DS) as polyanion. One film was composed with 4 bilayers of (DEAE-DS)4 and was labeled D−. The other film was the same as D− but with an added terminal layer of DEAE polycation: (DEAE-DS)4-DEAE (labeled D+). The dynamic adsorption/desorption of proteins on the films were characterized by dynamic contact angle (DCA) and atomic force microscopy (AFM). Human endothelial cell (HUVEC) adhesion and proliferation were quantified and correlated to protein adsorption analyzed by DCA for fibronectin, vitronectin, and bovine serum albumin (BSA). Our results showed that the endothelial cell response was optimal for films composed of DS as external layer. Fibronectin was found to be the only protein to exhibit a reversible change in conformation after desorption test. This behavior was only observed for (DEAE-DS)4 films. (DEAE-DS)4 films could enhance HUVEC proliferation in agreement with fibronectin ability to easily change from conformation.

This study deals with the anchorage of polyelectrolyte films onto titanium surfaces via a cathecol-based linker for biomedical applications. The following study uses a molecule functionalized with a catechol and a carboxylic acid: 3-(3,4-dihydroxyphenyl)propanoic acid. This molecule is anchored to the TiO.sub.2 substrate via the catechol while the carboxylic acid reacts with polymers bearing amine groups. By providing a film anchorage of chemisorption type, it makes possible to deposit polyelectrolytes on the surface of titanium. This method based on catechol anchorage of polyelectrolytes open a window towards large possibilities of clinical applications.

This study deals with the anchorage of polyelectrolyte films onto titanium surfaces via a cathecol-based linker for biomedical applications. The following study uses a molecule functionalized with a catechol and a carboxylic acid: 3-(3,4-dihydroxyphenyl)propanoic acid. This molecule is anchored to the TiO.sub.2 substrate via the catechol while the carboxylic acid reacts with polymers bearing amine groups. By providing a film anchorage of chemisorption type, it makes possible to deposit polyelectrolytes on the surface of titanium. This method based on catechol anchorage of polyelectrolytes open a window towards large possibilities of clinical applications.

Understanding the mechanism of the bacterial cell adhesion to solid surfaces is of great medical and industrial importance. Bacterial adhesion to inert surfaces, such as a catheter, and other indwelling devices can form biofilm, consequently cause severe morbidity and often fatal infections. Initial bacterial adhesion to the material surfaces is a complicated process that is affected by various physicochemical properties of both bacterial cells and substratum surfaces. The surface properties of the cells were characterized by the sessile drop technique. Moreover, the interfacial free energy of Staphylococcus aureus adhesion to the supporting materials was determined. The results showed that S. aureus examined at different pH levels could be considered hydrophilic. We noted hat the electron-donor character of S. aureus was important at intermediate pH (pH 5, pH 7, and pH 9) and it decreased at both limits acidic and basic conditions. In addition, the adhesion of Staphylococcus aureus ATCC 25923 to the hydrophilic glass and hydrophobic indium tin oxide (ITO)-coated glass surfaces at different pH values (2, 3, 5, 7, 9 and 11) was investigated using atomic force microscopy (AFM) and image analysis was assessed with the Mathlab(A (R)) program. The data analysis showed that cells (number of adhering cells to glass and ITO-coated glass surface) adhered strongly at acidic pH and weakly at alkaline pH. Also, S. aureus has the ability to attach to both hydrophobic and hydrophilic surfaces, but the adhesion was higher on hydrophobic surface.

Maps developed using Akima’s interpolation method were used to compare patterns of within-tree variation for Pinus taeda L. (loblolly pine) wood properties in plantation-grown trees aged 13 and 22 years. Air-dry density, microfibril angle (MFA) and modulus of elasticity (MOE) maps represented the average of 18 sampled trees in each age class. Near infrared (NIR) spectroscopy models calibrated using SilviScan provided data for the analysis. Zones of high density, low MFA and high MOE wood increased markedly in size in maps of the older trees. The proportion of wood meeting the visually graded No. 1 (11 GPa) and No. 2 (9.7 GPa) MOE design values for southern pine lumber increased from 44 to 74% and from 58 to 83% respectively demonstrating the impact of age on end-product quality. Air-dry density increased from pith to bark at all heights but lacked a significant trend vertically, while radial and longitudinal trends were observed for MFA and MOE. Changes were consistent with the asymptotic progression of properties associated with full maturity in older trees.

Maps developed using Akima’s interpolation method, and representing average data for trees aged 13 and 22 years, were used to compare patterns of within-tree variation for Pinus taeda L. (loblolly pine) tracheid properties: coarseness ( C ), specific surface ( S ), radial ( R ) and tangential ( T ) diameter and wall thickness ( w ). SilviScan-calibrated near-infrared (NIR) spectroscopy provided data for the analysis with C ( R c 2  = 0.85, R p 2  = 0.85), S ( R c 2  = 0.83, R p 2  = 0.76), and w ( R c 2  = 0.89, R p 2  = 0.93) models having very good calibration / prediction statistics, while those for T and R diameter were moderate ( R c 2  = 0.79, R p 2  = 0.57) and poor ( R c 2  = 0.64, R p 2  = 0.19), respectively. C , S , and w maps were similar to the density maps for P. taeda and indicate the properties increase radially at all heights. The T diameter map was similar to maps reported for microfibril angle except that T diameter increased radially and with height whereas microfibril angle decreased radially and with height. The map for R diameter increased with height and was unlike the other properties examined; caution is recommended regarding any interpretations based on the R diameter map owing to the weak statistics observed for the NIR model. Changes observed between the two ages are consistent with the asymptotic progression of properties associated with maturation.