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Molecular tools have shown an added value in the diagnosis of infectious diseases, in particular for those caused by fastidious intracellular microorganisms, or in patients receiving antibiotics before sampling. If 16S rDNA amplification had been gradually implemented in microbiology laboratories, specific real-time polymerase chain reaction (PCR) would have permitted an increase in the sensitivity of molecular methods and a reduction of contamination. Herein, we report our experience in the diagnosis of infectious diseases over two years, during which 32,948 clinical samples from 18,056 patients were received from France and abroad. Among these samples, 81,476 PCRs were performed, of which 1,192 were positive. Molecular techniques detected intracellular microorganisms in 31.3 % of respiratory samples, 27.8 % of endocarditis samples and 51.9 % of adenitis samples. Excluding intracellular bacteria, 25 % of the positive samples in this series were sterile in culture. Conventional broad-range PCR permitted the identification of fastidious and anaerobic microorganisms, but specific real-time PCR showed a significant superiority in the diagnosis of osteoarticular infections, in particular for those caused by Kingella kingae and Staphylococcus aureus , and for endocarditis diagnosis, specifically when Streptococcus gallolyticus and Staphylococcus aureus were involved. The sensitivity of conventional broad-range PCR was 62.9 % concerning overall diagnoses for which both techniques had been performed. These findings should lead microbiologists to focus on targeted specific real-time PCR regarding the clinical syndrome. Finally, syndrome-driven diagnosis, which consists of testing a panel of microorganisms commonly involved for each syndrome, permitted the establishment of 31 incidental diagnoses.

Système d’analyse fournissant des renseignements atmosphériques à la neige (SAFRAN) is a mesoscale atmospheric analysis system for surface variables. It produces an analysis at the hourly time step using ground data observations. One of SAFRAN’s main features is that it is based on climatically homogeneous zones and is able to take vertical variations into account. Originally intended for mountainous areas, it was later extended to cover France. This paper focuses on the validation of the extended version. The principle of the analysis is described and its quality was tested for five parameters (air temperature, humidity, wind speed, rainfall, and incoming radiation), using Météo-France’s observation network and data of some well-instrumented stations. Moreover, SAFRAN’s rainfall was compared with another analysis, known as analyse utilisant le relief pour l’hydrométéorologie (Aurelhy). Last, two different versions of SAFRAN were compared for mountain conditions. Temperature and relative humidity were well reproduced, presenting no bias. Wind speed was also well reproduced; however, its bias was −0.3 m s−1. The interpolation from the 6-h time step of the analysis to the 1-h time step was one of the sources of error. The precipitation analysis was robust and not biased; its root-mean-square error was 2.4 mm day−1. This error was mainly due to the spatial heterogeneity of the precipitation within the geographical zones of analysis (1000 km²). The analysis of incoming solar radiation presented some biases, especially in coastal areas. The results of the comparison with some well-instrumented sites were encouraging. SAFRAN is being run operationally at Météo-France on a real-time basis for various applications.

Our Solar System was formed from a cloud of gas and dust. Most of the dust mass is contained in amorphous silicates 1 , yet crystalline silicates are abundant throughout the Solar System, reflecting the thermal and chemical alteration of solids during planet formation. (Even primitive bodies such as comets contain crystalline silicates 2 .) Little is known about the evolution of the dust that forms Earth-like planets. Here we report spatially resolved detections and compositional analyses of these building blocks in the innermost two astronomical units of three proto-planetary disks. We find the dust in these regions to be highly crystallized, more so than any other dust observed in young stars until now. In addition, the outer region of one star has equal amounts of pyroxene and olivine, whereas the inner regions are dominated by olivine. The spectral shape of the inner-disk spectra shows surprising similarity with Solar System comets. Radial-mixing models naturally explain this resemblance as well as the gradient in chemical composition. Our observations imply that silicates crystallize before any terrestrial planets are formed, consistent with the composition of meteorites in the Solar System.

The aim of this study was to investigate the acute effects of whole-body vibration exercises (WBVE) in different positions on muscular activity of flexor digitorum superficialis (FD), wrist extensor (ED), and handgrip strength (HG) of healthy men. Fifteen participants have performed 5 test sets each one consisting of HG strength measurement and 1-minute WBVE intervention (frequency: 50 Hz, amplitude: 1.53 mm, synchronous tri-planar oscillating/vibratory platform), that could be control (no exposition to vibration), squat (30 seconds of rest and 30 seconds of WBVE in squat position), or push-up (30 seconds of rest, and 30 seconds of WBVE in push-up position). After testing, participants had 2 minutes of rest and then were encouraged to keep themselves on a pull-up bar for 30 seconds. During all procedures, muscular activity of FD and ED was measured by surface electromyography (EMG). Statistical analysis has revealed that the EMG measured in the FD during the static pull-up bar exercise after SQUAT condition was significantly higher (P = .004) than the CONTROL and PUSH-UP conditions. Whole-body vibration exercises in squat position increase acutely muscle activation of the FD during isometric exercises of longer duration, while muscle activation of ED and HG strength are not affected by WBVE.

A growing body of evidence attributes properties of chemo- and/or radiation-resistance to cancer stem cells (CSCs). Moreover, non-targeted delayed effects such as genomic instability, transmitted through many generations, can be observed in the progeny of surviving irradiated cells. As a consequence, we propose that radiation-resistance properties associated to CSCs could confer a key role to this subpopulation in the transmission of genomic instability. To test this hypothesis, we searched the CSC markers associated to radiation-resistance in breast cancer cell lines and studied the role of the resistant cells in the transmission of genomic instability. First, we show that irradiation induces a 2-4 weeks period of intense cell death leading to the emergence of chromosomal unstable cells during more than 35 population doublings. Then, among seven breast CSC markers, we identify CD24(-/low) labelling as a marker of radiation-resistance. We demonstrate that CD24(+) progeny of irradiated cells exclusively descends from CD24(-/low) cells. Finally, we show that delayed chromosomal instability is only expressed by CD24(+) cells, but is transmitted by stable surviving CD24(-/low) cells. So, for the first time a CSC marker, CD24, is associated with the transmission of genomic instability. This work may assign a new deleterious role to breast CSCs in aggressive recurrence after radiotherapy, as the transmitted genomic instability potentially leads tumour cells to acquire more aggressive characteristics.

Mode I fracture characterization was performed in wood using the single-edge-notched beam loaded in three-point-bending (SEN-TPB). A data reduction scheme based on equivalent linear elastic fracture mechanics concept was used to evaluate the Resistance -curve instead of classical methods. The method is founded on assessment of an equivalent crack from specimen compliance using beam theory and the existence of a stress relief region in the crack vicinity. Crack length monitoring is unnecessary during the loading process, providing a complete Resistance -curve which is essential for a clear identification of the fracture energy. Experimental results were obtained from fracture tests involving geometrically similar SEN-TPB specimens of different sizes. It was observed that the smallest tested specimen is inadequate to estimate the fracture energy due to fracture process zone confinement. Contrarily, the other two are suitable for such purpose. An inverse method was used to determine a bilinear cohesive law representative of wood material fracture. It was concluded that a unique cohesive law is able to mimic the fracture behaviour of considered specimen sizes.

Superoxide (O 2 ·− ) overproduction, by decreasing the nitric oxide ( · NO) bioavailability, contributes to vascular complications in type 1 diabetes. In this disease, the vascular O 2 ·− can be produced by the NADPH oxidase (NOX), nitric oxide synthase (NOS), and xanthine oxidase (XO). This study aimed to determine the contribution of each enzymatic pathway in hyperglycemia-induced O 2 ·− overproduction, and the effects of an endurance training program and insulin therapy, associated or not, on the O 2 ·− production (amount and related enzymes) in diabetic rats. Forty male Wistar rats were divided into diabetic (D), diabetic treated with insulin (D-Ins), diabetic trained (D-Tr), or diabetic insulin-treated and trained (D-Ins + Tr) groups. An additional healthy group was used as control. Insulin therapy (Glargine Lantus, Sanofi) and endurance training (treadmill run: 60 min/day, 25 m/min, 5 days/week) started 1 week after diabetes induction by streptozotocin (45 mg/kg), and lasted for 8 weeks. At the end of the protocol, the O 2 ·− production in aorta rings was evaluated by histochemical analyses (DHE staining). Each production pathway was studied by inhibiting NOX (apocynin), NOS (L-Name), or XO (allopurinol) before DHE staining. Diabetic rats exhibited hyperglycemia-induced O 2 ·− overproduction, resulting from NOX, NOS, and XO activation. Insulin therapy and endurance training, associated or not, decreased efficiently and similarly the O 2 ·− overproduction. Insulin therapy reduced the hyperglycemia and decreased the three enzymatic pathways implicated in the O 2 ·− production. Endurance training decreased directly the NOS and XO activity. While both therapeutic strategies activated different pathways, their association did not reduce the O 2 ·− overproduction more significantly.

Background: Glucocorticoid induced osteoporosis (GIOP) is one of the most important causes of morbidity in lupus individuals. Whole body vibration exercises (WBVE) may be a safe alternative to prevent and amend muscular and bone damage, and decrease muscle related risk factors for falls. It is possible to evaluate neuromuscular responses to the WBVE through surface electromyography (sEMG). Objective: To analyze and compare the acute responses of the WBVE on sEMG of lower limbs of female systemic lupus erythematosus (SLE) individuals with chronic glucocorticoid use with and without bone impairments and non-lupus controls. Methods: All patients (non-lupus and with SLE) had a dual-energy X-ray absorptiometry (DXA) scan (body composition, bone composition right hip, lumbar segment and whole body). After DXA, they were divided into three groups: SLE with osteopenia (OPIA) (SLE OPIA), SLE without OP or OPIA (SLE) and non-lupus individuals as control (CG). Twenty-seven women were submitted to WBVE, on different frequencies with the same amplitude. The experiment was performed over two days, 48 h apart. The individuals stood at a half squat position on a vertical vibrating platform at different frequencies with the same amplitude on both days. Vastus lateralis (VL), gastrocnemius medialis (GM) and tibialis anterioris (TA) sEMG analyses were undertaken simultaneously while performing the exercises, in a randomized manner. Results: There were no differences between sarcopenia index among groups, despite the bone impairment of the SLE OPIA group. The greatest muscle activation occurred in the lower frequency applied for VL. A group x frequency difference was found only for GM (p = 0.034; η2 = 0.272). Conclusion: The results indicate that lupus individuals have similar neuromuscular activity to the WBVE as non-lupus controls. Moreover, this suggests that WBVE is a safe and viable physical exercise for lupus individuals with chronic glucocorticoid induced osteoporosis.

Magnetic resonance contrast agents have demonstrated their clinical usefulness in a variety of organs for improved detection of various neoplastic, inflammatory and functional abnormalities. Gadolinium chelates are the most widely used. They are extracellular, non-specific contrast agents. Their use in many clinical indications is justified because, in conjunction with improved imaging techniques, these safe and image-enhancing contrast agents add morphologic and functional information compared with unenhanced MR images. This article describes the commercially available compounds, and summarizes their approval status on the international market regarding indications and doses. Their mechanisms of action, biodistributions, toxicities and tolerance profiles in normal and high-risk patient populations are described. Additionally, this article reviews the specific recommendations by the manufacturers for patients at risk. Finally, their main clinical applications are reviewed.

Background. A deficiency of D-chiro-inositol-inositolphosphoglycan mediator (DCI-IPG) may contribute to insulin resistance in polycystic ovary syndrome (PCOS). Whether the relationship between impaired DCI-IPG release and insulin resistance is specific to PCOS rather than obesity is unknown. We assessed insulin-released DCI-IPG and its relationship to insulin sensitivity at baseline and after weight loss in obese women with and without PCOS. Methods. Obese PCOS ( n = 16 ) and normal ( n = 15 ) women underwent 8 weeks of a hypocaloric diet. The Matsuda index, area under the curve DCI-IPG ( AUC DCI-IPG ), AUC insulin , and AUC DCI-IPG / AUC insulin were measured during a 2 hr OGTT at baseline and 8 weeks. Results. PCOS women had lower AUC DCI-IPG / AUC insulin at baseline and a significant relationship between AUC DCI-IPG / AUC insulin and Matsuda index ( p = 0.0003 ), which was not present in controls. Weight loss was similar between PCOS (−4.08 kg) and normal women (−4.29 kg, p = 0.6281 ). Weight loss in PCOS women did not change the relationship between AUC DCI-IPG / AUC insulin and Matsuda index ( p = 0.0100 ), and this relationship remained absent in control women. Conclusion. The association between AUC DCI-IPG / AUC insulin and insulin sensitivity was only found in PCOS but not in normal women, and this relationship was unaffected by weight loss. DCI and its messenger may contribute to insulin resistance in PCOS independent of obesity.