Explore the vast range of titles available.

• Microclimatic effects (light, temperature) are often neglected in phenological studies and little information is known about the impact of resource availability (nutrient and water) on tree’s phenological cycles. • Here we experimentally studied spring and autumn phenology in four temperate trees in response to changes in bud albedo (white-painted vs black-painted buds), light conditions (nonshaded vs c. 70% shaded), water availability (irrigated, control and reduced precipitation) and nutrients (low vs high availability). • We found that higher bud albedo or shade delayed budburst (up to +12 d), indicating that temperature is sensed locally within each bud. Leaf senescence was delayed by high nutrient availability (up to +7 d) and shade conditions (up to +39 d) in all species, except oak. Autumn phenological responses to summer droughts depended on species, with a delay for cherry (+7 d) and an advance for beech (−7 d). • The strong phenological effects of bud albedo and light exposure reveal an important role of microclimatic variation on phenology. In addition to the temperature and photoperiod effects, our results suggest a tight interplay between source and sink processes in regulating the end of the seasonal vegetation cycle, which can be largely influenced by resource availability (light, water and nutrients).

Increased summer drought will exacerbate the regeneration of many tree species at their lower latitudinal and altitudinal distribution limits. In vulnerable habitats, introduction of more drought-tolerant provenances or species is currently considered to accelerate tree species migration and facilitate forest persistence. Trade-offs between drought adaptation and growth plasticity might, however, limit the effectiveness of assisted migration, especially if introductions focus on provenances or species from different climatic regions. We tested in a common garden experiment the performance of Pinus sylvestris seedlings from the continental Central Alps under increased temperatures and extended spring and/or summer drought, and compared seedling emergence, survival and biomass allocation to that of P. sylvestris and closely related Pinus nigra from a Mediterranean seed source. Soil heating had only minor effects on seedling performance but high spring precipitation doubled the number of continental P. sylvestris seedlings present after the summer drought. At the same time, twice as many seedlings of the Mediterranean than the continental P. sylvestris provenance were present, which was due to both higher emergence and lower mortality under dry conditions. Both P. sylvestris provenances allocated similar amounts of biomass to roots when grown under low summer precipitation. Mediterranean seedlings, however, revealed lower phenotypic plasticity than continental seedlings under high precipitation, which might limit their competitive ability in continental Alpine forests in non-drought years. By contrast, high variability in the response of individual seedlings to summer drought indicates the potential of continental P. sylvestris provenances to adapt to changing environmental conditions.

Climate change is expected to significantly alter forest ecosystems, reducing the suitability of the key economic tree species Norway spruce ( Picea abies ) and European beech ( Fagus sylvatica ) in low- and mid-elevation forests of Central Europe. As these species face increasing pressures from drought, storms, and pests, it is crucial to identify alternative tree species that are economically viable and capable of maintaining primary ecosystem services. This study investigated the potential of Douglas fir ( Pseudotsuga menziesii ), a non-native conifer, to establish from seed and compete with native broadleaf and conifer species during the early regeneration stage under differing resource availabilities. We assessed the growth performance and phenotypic plasticity of Douglas fir seedlings over three years in a controlled common-garden experiment. Seedlings of Douglas fir, along with seven native species — Norway spruce, silver fir ( Abies alba ), Scots pine ( Pinus sylvestris ), European beech, pedunculate oak ( Quercus robur ), sessile oak ( Q. petraea ), and sycamore ( Acer pseudoplatanus ) — were grown for three years under factorial combinations of high and low availabilities of light, nutrients, and water. Seedling height, biomass allocation to shoots and roots and phenotypic plasticity of these traits were measured to evaluate the competitive ability of individual species and their potential to adapt to changing environmental conditions. While Douglas fir seedlings exhibited strong growth performance compared to the conifers Norway spruce and silver fir, their biomass production and height growth was considerably lower than that of the broadleaved sycamore and beech. However, Douglas fir’s height growth rate in the third year exceeded all species except sycamore. This was particularly pronounced under dry and/or nutrient-poor conditions, indicating a potential competitive advantage under expected future climatic conditions. In agreement with field studies, our results indicate that non-native Douglas fir may sustainably establish in dry, nutrient poor European lowland forests due to its superior early growth performance under these conditions and the high phenotypic plasticity, of its root system. This holds especially in situations where the species competes with other conifers, while its ability to successfully compete with broadleaves appears to be largely restricted to nutrient-poor sites.

In diabetes mellitus, hyperglycemia promotes the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs) to enhance medial vascular calcification, a common complication strongly associated with cardiovascular disease and mortality. The mechanisms involved are, however, still poorly understood. Therefore, the present study explored the potential role of serum- and glucocorticoid-inducible kinase 1 (SGK1) during vascular calcification promoted by hyperglycemic conditions. Exposure to high-glucose conditions up-regulated the SGK1 expression in primary human aortic VSMCs. High glucose increased osteogenic marker expression and activity and, thus, promoted the osteogenic transdifferentiation of VSMCs, effects significantly suppressed by additional treatment with the SGK1 inhibitor EMD638683. Moreover, high glucose augmented the mineralization of VSMCs in the presence of calcification medium, effects again significantly reduced by SGK1 inhibition. Similarly, SGK1 knockdown blunted the high glucose-induced osteogenic transdifferentiation of VSMCs. The osteoinductive signaling promoted by high glucose required SGK1-dependent NF-κB activation. In addition, advanced glycation end products (AGEs) increased the SGK1 expression in VSMCs, and SGK1 inhibition was able to interfere with AGEs-induced osteogenic signaling. In conclusion, SGK1 is up-regulated and mediates, at least partly, the osteogenic transdifferentiation and calcification of VSMCs during hyperglycemic conditions. Thus, SGK1 inhibition may reduce the development of vascular calcification promoted by hyperglycemia in diabetes.

Fires shape fundamental properties of many forest ecosystems and climate change will increase their relevance in regions where fires occur infrequently today. In ecosystems that are not adapted to fire, post-fire tree recruitment is often sparse, a fact that might be attributed to a transient lack of mycorrhizae. Ectomycorrhizal (EcM) fungi play an important role for recruitment by enhancing nutrient and water uptake of their hosts. The questions arise whether and for how long the EcM community is transformed by fire. We investigated the resistance and resilience of EcM fungal communities on a chronosequence of 12 Pinus sylvestris stands in Valais (Switzerland) and Val d'Aosta (Italy) affected by fire between 1990 and 2006. Soil samples from burnt and non-burnt forests were analyzed with respect to EcM fungi by means of a bioassay. The number of EcM species was significantly lower in samples from recently (2-5 years) burnt sites than nonburnt forest, and increased with time since fire reaching levels of adjacent forests after 15-18 years. Community composition changed after fire but did not converge to that of non-burnt sites over the 18 year period. Only Rhizopogon roseolus and Cenococcum geophilum were abundant in both burnt sites and adjacent forest. Our data indicate fire resistance of some EcM fungal species as well as rapid resilience in terms of species number, but not in species composition. As long as the function of different EcM species for seedling establishment is unknown, the consequences of long-term shifts in EcM community composition for tree recruitment remain unclear.

Temperature manipulation experiments are an effective way for testing plant responses to future climate conditions, especially for predicting shifts in plant phenological events. While passive warming techniques are widely used to elevate temperature in low stature plant communities, active warming has been applied less frequently due to the associated resource requirements. In forest ecosystems, however, active warming is crucial to simulate projected air temperature rises of 3–5 K, especially at the warm (i.e., southern and low elevation) range edges of tree species. Moreover, the warming treatment should be applied to the complete height of the experimental plants, e.g., regenerating trees in the understory. Here, we combined open top chambers (OTCs) with active heat sources, an electric heater (OTC-EH) and warming cables (OTC-WC), and tested the effectiveness of these set-ups to maintain constant temperature differences compared to ambient temperature across 18 m 2 plots. This chamber size is needed to grow tree saplings in mixture in forest gaps for 3 to 10 years. With passive warming only, an average temperature increase of approx. 0.4 K as compared to ambient conditions was achieved depending on time of the day and weather conditions. In the actively warmed chambers, average warming exceeded ambient temperatures by 2.5 to 2.8 K and was less variable over time. However, active warming also reduced air humidity by about 15%. These results underline the need to complement passive warming with active warming in order to achieve constant temperature differences appropriate for climate change simulations under all weather conditions in large OTCs. Since we observed considerable horizontal and vertical temperature variation within OTCs with temperature differences of up to 16.9 K, it is essential to measure and report within-plot temperature distribution as well as temporal temperature variation. If temperature distributions within large OTCs are well characterized, they may be incorporated in the experimental design helping to identify non-linear or threshold responses to warming.

(1) Understanding tree seedling responses to water, nutrient, and light availability is crucial to precisely predict potential shifts in composition and structure of forest communities under future climatic conditions. (2) We exposed seedlings of widespread Central European tree species with contrasting leaf habit, deciduous broadleaves (DB) and evergreen conifers (EC), to factorial combinations of manipulated precipitation (100% and 50% of ambient), shade (40% and 60% of full sunlight), and nutrient availability (low and high NPK), and measured specific leaf area, C/N ratio, soluble sugars, starch and non-structural carbohydrate concentration, and δ13C of the leaves. (3) We found contrasting effects of water and nutrient availability on foliar traits of the two species groups: EC exhibited higher tolerance to low resource availability but also less plasticity in foliar traits, which is congruent with a “slow” resource strategy. In contrast, foliage of DB reacted particularly to altered nutrient availability, corresponding to a “fast” resource strategy with high foliar plasticity and rapid adjustments to resource fluctuations, commonly adopted by species with high growth rates. (4) We conclude that DB will respond to environmental change with foliar acclimation, while EC will either tolerate, to some extent, or shift their distribution range in response to environmental change.

Even if the approach accommodates variability in the localization of individual participants' activations, the analysis remains an assessment of group-level consistency, and is therefore necessarily subject to the usual concerns regarding statistical power (the problems caused by small sample sizes, including how they have a deleterious impact on the literature by inflating apparent effect-sizes, are discussed in Button et al., 2013). The claim is made that this compensates for the small sample size by estimating a putative impact of expertise, however no analysis of this is presented. [...]participants' language combinations are not as well-matched as claimed. The paper contains some genuine issues beyond those acknowledged that we worry fundamentally undermine the conclusions: real effects are likely to have been missed due to lack of power, the participant selection introduced unnecessary sources of variability (age and expertise), the selection of materials means that the reported effects may not relate to SI but to consecutive interpretation and the constrained analysis space rules out conclusions about the broader language control network.

Although the Burgundy truffle (Tuber aestivum) is an ectomycorrhizal fungus of important economic value, its subterranean life cycle and population biology are still poorly understood.Here, we determine mating type and simple sequence repeat (SSR) maternal genotypes of mapped fruiting bodies to assess their genetic structure within two naturally colonized forest sites in southern Germany. Forty-one genotypes were identified from 112 fruiting bodies. According to their mating types, the maternal genotypes were aggregated only in one population. Genotypic diversity of individuals that mostly were small and occurred in 1 out of 2 years of sampling was high. Although these results suggested a ruderal colonization strategy, some genets spread several hundred meters.This result indicates that, besides sexual spore dispersal, vegetative growth or spreading by mycelial propagules contributes to dissemination. In one site, fewer individuals with a tendency to expand genets belonging to only one genetic group were observed. In the second site, numerous small individuals were found and were grouped into two clearly differentiated genetic groups that were spatially intermingled. Forest characteristics and disturbances are possible reasons for the observed genetic patterns.Our findings contribute to a better understanding of the biology of one of the most widespread and commercially important truffle species. This knowledge is critical for establishing and maintaining sustainable long-term truffle cultivations.

Climate change alters both water and CO 2 availability for plants, but it is largely unknown how they interact with light to affect tree seedling establishment and early growth. Light availability is often regulated by forest management, thus understanding how these resources co‐limit the regeneration success of tree species and populations with contrasting drought tolerances is essential for adaptive forest management and particularly for assisted migration. We studied biomass partitioning of 3‐year‐old Scots pine ( Pinus sylvestris ) and European black pine ( Pinus nigra ) seedlings in response to combined effects of light (22% and 40% shade), soil water availability (moist and dry conditions) and CO 2 (ambient and elevated), and examined the responses of seedlings from Central Alpine and Mediterranean origin. Seedlings of nine populations with varying drought tolerances were grown in a common garden in the European Central Alps. Shoot height, vertical root length, shoot and root biomass of the plants were assessed at the end of the third growing season. Under 40% shade and dry conditions, P. sylvestris seedlings severely reduced shoot biomass, resulting in an increased specific shoot height ( SSH ) compared to seedlings under 22% shade and moist conditions. In contrast, P. nigra seedlings retained a constant shoot biomass under all treatment combinations. Seedlings from drier origin were generally larger, heavier and had longer vertical roots than those from wetter locations. In order to keep up shoot height, seedlings from wetter origins disproportionately increased SSH under shaded conditions compared to populations from drier origin. Synthesis and applications . Under high light availability, Scots pine ( Pinus sylvestris ) and European black pine ( Pinus nigra ) seedlings were well adapted to dry conditions. Moderate shading, however, substantially reduced Scots pine but not black pine growth, and potentially amplified the vulnerability of Scots pine seedlings to drought. Optimising light conditions in forests, for example by thinning, may thus enhance early Scots pine regeneration in a drier future climate. Under high light availability, Scots pine ( Pinus sylvestris ) and European black pine ( Pinus nigra ) seedlings were well adapted to dry conditions. Moderate shading, however, substantially reduced Scots pine but not black pine growth, and potentially amplified the vulnerability of Scots pine seedlings to drought. Optimising light conditions in forests, for example by thinning, may thus enhance early Scots pine regeneration in a drier future climate.