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Perinatal depression (PND) is a significant contributor to maternal morbidity globally. Recognized as a major cause of poor infant development, epidemiological and interventional research on it has increased over the last decade. Recently, studies have pointed out that PND is a heterogeneous condition, with variability in its phenotypes, rather than a homogenous latent entity and a concrete diagnosis, as previously conceptualized in psychometric literature and diagnostic systems. Therefore, it is pertinent that researchers recognize this to progress in elucidating its aetiology and developing efficacious interventions. This systematic review is conducted in accordance with the Meta-analysis of observational studies in epidemiology (MOOSE). It aims to provide an updated and comprehensive account of research on heterogeneity in phenotypes of PND and its implications in research, public health, and clinical practice. It provides a synthesis and quality assessment of studies reporting heterogeneity in PND using cutting-edge statistical techniques and machine learning algorithms. After reporting the phenotypes of PND, based on heterogeneous trajectories and symptom profiles, it also elucidates the risk factors associated with severe forms of PND, followed by robust evidence for adverse child outcomes. Furthermore, recommendations are made to improve public health and clinical practice in screening, diagnosis, and treatment of PND.

This study investigated the adsorption of Oxytetracycline (OTC) from pharmaceutical wastewater using a kappa carrageenan based hydrogel (KPB). The aim of the present study was to explore the potential of KPB for long-term pharmaceutical wastewater treatment. A sustainable adsorbent was developed to address oxytetracycline (OTC) contamination. The hydrogel’s structural and adsorption characteristics were examined using various techniques like Scanning Electron Microscope (SEM), Fourier Transform Infrared (FTIR), X-ray powder diffraction (XRD), and kinetic models. The results revealed considerable changes in the vibrational modes and adsorption bands of the hydrogel, suggesting the effective functionalization of Bentonite nano-clay. Kappa carrageenan based hydrogel achieved the maximum removal (98.5%) of OTC at concerntration of 40 mg/L, pH 8, cotact time of 140 min and adsorbent dose of 0.1 g (KPB-3). Adsorption of OTC increased up to 99% with increasing initial concentrations. The study achieved 95% adsorption capacity for OTC using a KPB film at a concentration of 20 mg/L and a 0.1 g adsorbent dose within 60 min. It also revealed that chemisorptions processes outperform physical adsorption. The Pseudo-Second-Order model, which emphasized the importance of chemical adsorption in the removal process, is better suited to represent the adsorption behavior. Excellent matches were found that R 2  = 0.99 for KPB-3, R 2  = 0.984 for KPB-2 and R 2  = 0.989 for KPB-1 indicated strong chemical bonding interactions. Statisctical analysis (ANOVA) was performed using SPSS (version 25) and it was found that pH and concentration had significant influence on OTC adsorption by the hydrogel, with p-values less than 0.05. The study identified that a Kappa carrageenan-based hydrogel with bentonite nano-clay and polyvinyl alcohol (PVA) can efficiently remove OTC from pharmaceutical effluent, with a p-value of 0.054, but weak positive linear associations with pH, temperature, and contact time. This research contributed to sustainable wastewater treatment and environmental engineering.

A radio-resistant bacterium labeled as strain TMC-6 was isolated from Thal desert, Pakistan and identified through 16S rRNA gene sequencing as Bacillus indicus strain TMC-6 (MN721293). The isolate was found to be resistant to UV radiation dose of 6.780 × 103 J/m2 and showed 50% survivability to mitomycin C (6 μg/ml) and H2O2 (30 mM). The bacterium showed yellowish orange coloration when grown on tryptone yeast glucose (TGY) medium. The cellular metabolite was extracted in methanol and purified through solid phase extraction with C18 column cartridge. The compound was characterized through UV/Visible spectrophotometry, Fourier Transform Infra-Red (FT-IR) spectroscopy and Liquid Chromatography Mass Spectrometry (LC–MS). The LC–MS analysis of the compound revealed a molar mass of 769 [m/z]− that matched the chemical formula C34H42O20 and identified as a glycosylated flavonoid xanthorhamnin. The compound showed significant antioxidant (77.05%) and metal chelation (79.80%) activities. Xanthorhamnin showed promising oxidative damage inhibitory actions in bovine serum albumin (65.32%) and mice liver lipids (71.61%) and prevented DNA strand breaks from oxidative stress. Cytotoxicity in brine shrimp larvae was observed when compared with mitomycin C indicating its effect toward cancerous cells. These findings concluded that xanthorhamnin from radio-resistant Bacillus indicus strain TMC-6 has high antioxidant, radioprotective, and antitumor properties against UV-mediated oxidative damages.

Microplastic pollution has garnered global attention in recent decades due to its recognized ecological concerns through previous studies. However, in Pakistan, scarce information has been reported on MP pollution concerning the freshwater ecosystem. The current study was conducted on Kallar Kahar Lake, Punjab, Pakistan for (1) quantification, characterization, and distribution of MPs in surface water, sediments, and fish samples and (2) two treatment processes (magnetization and coagulation + flocculation) for the removal of MPs from the water. Samples were collected from each point by grab sampling method to investigate the MPs according to their type, shape, and color. The MP quantification and analysis were accomplished via the counting method by a stereomicroscope and Fourier transform infrared spectroscopy for their polymer type and composition. Results indicated the average MP abundance as 49.6 ± 11.14 MP/500 mL, 143 ± 48.18 MP/100 g, and 79 ± 12.2 items for water, sediments, and fish correspondingly. The dominant MP colors were blue, transparent, and green in all three environmental compartments. The ATR-FTIR identified the polymer types in lake water, sediment, and fish were PPS, PIB, and PLF; PET, PE, PP, and Natural Latex Rubber; and PET, respectively. The MP removal rate was observed high in both treatments. The average % removal rate of iron ore magnetization treatment was observed to be 80% at 1300 mg/L dosage of Fe 2 O 3 . Similarly in chemical coagulation processes, the highest MP removal efficiency was 85% (PET), 83% (PPS) and 80% (PIB) at the different concentration dosages of 150 + 15 mg/L, 111 + 15 mg/L, and 150 + 111 + 15 mg/L for Combination 1, Combination 2, and Combination 3, respectively. Overall, this study provided an integrative and novel approach for the removal of MP from surface water, which also holds an explicit commercial utilization prospect to overpower the MP pollution in water bodies. Also, the current findings serve as baseline data for the study of local freshwater systems.

People high in depressive symptoms show probability bias: they think negative events are relatively likely, especially compared with positive events. However, their past- and future-related thinking also has other distinctive characteristics, so we wondered how independent probability bias is from these other phenomena. In two samples of Turkish students (Ns = 163 and 179), we found that depressive symptoms were the strongest predictor of probability bias even when rumination, intrusive future imagery, overgeneral memory and anxiety were controlled in our models; furthermore, probability bias remained a significant predictor of depression when these other variables were controlled. These results suggest that a relatively negative probability bias is an independent correlate of depressive symptoms, and is not simply confounded with other past- or future thinking-related phenomena that have been observed in more depressed individuals. We hope that future individual differences and clinical research will focus more on depression-related probability bias.

The present study observed the bearing of the third-person effect on individuals and perceptions of the media's influence on people's opinions regarding fake news. The third-person effect theory says that people would perceive media messages to have more effects on other people than on themselves; predicting self-other discrepancy in perceived influence of media messages. This study focuses on investigating perceived prevalence of fake news, and fake news checking habits of news consumers. Furthermore, with reference to third person effect the intensity of self-other discrepancy in the perceived effects of fake news at different levels was examined; also identifying variables predicting those discrepancies. An online survey of the university students was conducted using convenient sampling confirmed a strong TPE. Results showed the presence of self-other discrepancy demonstrating the tendency to consider others as more influenced by fake news and this perception intensifies with social distance.

This study aims to determine UV-B resistance and to investigate computational analysis and antioxidant potential of methoxy-flavones of Micromonospora aurantiaca TMC-15 isolated from Thal Desert, Pakistan. The cellular extract was purified through solid-phase extraction and UV–Vis spectrum analysis indicated absorption peaks at λmax 250 nm, 343 nm, and 380 nm that revealed the presence of methoxy-flavones named eupatilin and 5-hydroxyauranetin. The flavones were evaluated for their antioxidant as well as protein and lipid peroxidation inhibition potential using di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH), 2,4-dinitrophenyl hydrazine (DNPH), and thiobarbituric acid reactive substances (TBARS) assays, respectively. The methoxy-flavones were further studied for their docking affinity and interaction dynamics to determine their structural and energetic properties at the atomic level. The antioxidant potential, protein, and lipid oxidation inhibition and DNA damage preventive abilities were correlated as predicted by computational analysis. The eupatilin and 5-hydroxyauranetin binding potential to their targeted proteins 1N8Q and 1OG5 is − 4.1 and − 7.5 kcal/mol, respectively. Moreover, the eupatiline and 5-hydroxyauranetin complexes illustrate van der Waals contacts and strong hydrogen bonds to their respective enzymes target. Both in vitro studies and computational analysis results revealed that methoxy-flavones of Micromonospora aurantiaca TMC-15 can be used against radiation-mediated oxidative damages due to its kosmotrophic nature. The demonstration of good antioxidant activities not only protect DNA but also protein and lipid oxidation and therefore could be a good candidate in radioprotective drugs and as sunscreen due to its kosmotropic nature.

Nadeem, M (2022). Experiences of Parental Conflict: A Qualitative Study of Female Adolescents in Pakistan. (Master’s thesis in Health Psychology). Scientific supervisor: Assoc. prof. Giedrė Širvinskienė. Lithuanian University of Health Sciences, Academy of Medicine, Faculty of Public Health, Department of Health Psychology: Kaunas.This study explores the narratives of young female adolescents’ experiences of witnessing parental conflict. The sample of this study consisted of 5 females, within the age range of 18 – 21 years, residing in Pakistan.The data was collected using an interview technique, where a self-developed structured interview questionnaire was utilised. The collected data was exhaustively analysed using the Interpretational Phenomenological Analysis (IPA) technique.The results generated a wide variety of themes indicating 4 main experiences the participants went through during their exposure to parental conflict, which were as follows: dependency of spouses on each other, lack of emotional control, mediation methods and outcomes of parental conflict.The analysis of themes concluded that the participants received distress as witnesses of parental conflict. They had psychologically secured a position as a mature, more responsible member of the family by being protective siblings and thoughtful daughters in aiding intervention methods for conflicting situations between parents.

Molecular studies have confirmed the silent circulation of enterovirus (EntV) and hepatitis A virus in the environment, even in the absence of clinical manifestation. Viral pathogens are among the major causes of disease outbreaks, particularly in the bigger cities and both in the developed and underdeveloped nations. Between June 2016 - June 2017, 97 samples of drinking water, river water polluted with sewage and blood were selected and obtained from high risk communities in Pakistan. Negatively charged membrane filters were used to concentrate the virus, followed by the use of specific PCR primers set for quick identification of the waterborne viruses. Enteroviruses were recovered from 40%, 28.57% and 33.33% of river water polluted with sewage samples in Lahore, Islamabad and Rawalpindi, respectively, while the presence of 13.13% and 11.76% of viral load was also confirmed in the drinking water of Lahore and Rawalpindi, respectively. A high prevalence of HAV (12.5% and 21.05%) was also verified in the clinical samples. Phylogenetic analysis indicated close resemblance of HAV isolates with the Indian strains. This study is the first ever comparative analysis of the EntV and HAV isolated from environmental samples and clinical specimen on a molecular level. The parallel surveillance of EntV and HAV in the river water polluted with sewage, and clinical samples is quite helpful for controlling and reducing the disease burden of the waterborne illnesses.

Genetic variation in cytochrome P450 ( CYP) 2C9 is known to cause significant inter-individual differences in drug response and adverse effects. The frequencies of CYP2C9*2 and CYP2C9*3 , both of which are responsible for the low activity of the enzyme, are not known in the Pakistani population. Therefore, we screened various ethnic groups residing in Pakistan for these polymorphisms. A total of 467 healthy human volunteers were recruited from six major ethnicities of Pakistan after written informed consent. Our results indicate that about 20% of the Pakistani population has a genotype containing at least one low activity allele. Ethnic Punjabi and Pathan populations had the highest frequencies of wild type genotypes while Urdu, Seraiki, and Sindhi populations showed higher rates of both low activity genotypes. The Baloch population showed the highest rates of low activity genotypes with less than 50% of the samples showing wild type genotypes, suggesting that more than half of the Baloch population possesses low activity genotypes. The frequencies found in various ethnic groups in Pakistan were comparable with ethnicities in the South Asian region except for the Baloch population. These results suggest that pharmacogenetics screening for low activity genotypes may be a helpful tool for clinicians while prescribing medications metabolized by CYP2C9.