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This study aims at conducting the first science mapping analysis of the Mediterranean forest research in order to elucidate its research structure and evolution. We applied a science mapping approach based on co-term and citation analyses to a set of scientific publications retrieved from the Elsevier's Scopus database over the period 1980-2014. The Scopus search retrieved 2,698 research papers and reviews published by 159 peer-reviewed journals. The total number of publications was around 1% (N = 17) during the period 1980-1989 and they reached 3% (N = 69) in the time slice 1990-1994. Since 1995, the number of publications increased exponentially, thus reaching 55% (N = 1,476) during the period 2010-2014. Within the thirty-four years considered, the retrieved publications were published by 88 countries. Among them, Spain was the most productive country, publishing 44% (N = 1,178) of total publications followed by Italy (18%, N = 482) and France (12%, N = 336). These countries also host the ten most productive scientific institutions in terms of number of publications in Mediterranean forest subjects. Forest Ecology and Management and Annals of Forest Science were the most active journals in publishing research in Mediterranean forest. During the period 1980-1994, the research topics were poorly characterized, but they become better defined during the time slice 1995-1999. Since 2000s, the clusters become well defined by research topics. Current status of Mediterranean forest research (20092014) was represented by four clusters, in which different research topics such as biodiversity and conservation, land-use and degradation, climate change effects on ecophysiological responses and soil were identified. Basic research in Mediterranean forest ecosystems is mainly conducted by ecophysiological research. Applied research was mainly represented by land-use and degradation, biodiversity and conservation and fire research topics. The citation analyses revealed highly cited terms in the Mediterranean forest research as they were represented by fire, biodiversity, carbon sequestration, climate change and global warming. Finally, our analysis also revealed the multidisciplinary role of climate change research. This study provides a first holistic view of the Mediterranean forest research that could be useful for researchers and policy makers as they may evaluate and analyze its historical evolution, as well as its structure and scientific production. We concluded that Mediterranean forest research represents an active scientific field.

Rates of nitrogen transformations support quantitative descriptions and predictive understanding of the complex nitrogen cycle, but measuring these rates is expensive and not readily available to researchers. Here, we compiled a dataset of gross nitrogen transformation rates (GNTR) of mineralization, nitrification, ammonium immobilization, nitrate immobilization, and dissimilatory nitrate reduction to ammonium in terrestrial ecosystems. Data were extracted from 331 studies published from 1984–2022, covering 581 sites. Globally, 1552 observations were appended with standardized soil, vegetation, and climate data (49 variables in total) potentially contributing to the observed variations of GNTR. We used machine learning-based data imputation to fill in partially missing GNTR, which improved statistical relationships between theoretically correlated processes. The dataset is currently the most comprehensive overview of terrestrial ecosystem GNTR and serves as a global synthesis of the extent and variability of GNTR across a wide range of environmental conditions. Future research can utilize the dataset to identify measurement gaps with respect to climate, soil, and ecosystem types, delineate GNTR for certain ecoregions, and help validate process-based models.

The first step in planning the actions for climate change adaptation involves performing Climate Change Vulnerability Assessment (CCVA) to vulnerable populations and ecological systems. The study aims to perform a bibliometric analysis on the CCVA research field to identify how it was structured in terms of the mostly occurring research terms and their citation impact. A set of scientific papers related to CCVA (n = 8941) were retrieved via Elsevier’s Scopus database over the period 1996–2016 using specific keywords. The search was carried out in February 2017. The number of yearly papers published in CCVA research field increased after 2006, increasing more than six times in 2016. Our co-occurrences term analysis grouped four clusters related to (i) exposure, sensitivity, and quantitative methods for assessing CCVA, (ii) adaptive capacity and qualitative approaches, (iii) CCVA integrated approaches by combining participatory methods to quantitative ones, and (iv) collaboration and participation issues related to global change. The co-citation analysis showed that highly cited research terms were related to adaptation, exposure factors, and related changes and global change. This bibliometric analysis has provided an overall picture on the CCVA research field, particularly highlighting its utility for climate change adaptation.

Aims It has been reported that root exudates of Sorghum bicolor can inhibit nitrification in a bioassay using Nitrosomonas, and methyl 3-(4-hydroxyphenyl) propionate (MHPP) was identified as one of the nitrification inhibiting compounds. Therefore, we have investigated the effects of this compound on nitrogen dynamic, potential nitrification activity and on soil microorganisms. Methods We conducted soil incubation experiments using synthetic MHPP to evaluate its effect on changes in inorganic soil nitrogen pools, on nitrification activity and on abundance of ammonia-oxidizing bacteria and archaea. Addition of MHPP at two concentrations equivalent to 70 and 350 µg C g⁻¹ soil was compared to glucose as a carbon source and to the commercially available nitrification inhibitor dicyandiamide (DCD). Results Soil amended with the high dose of MHPP and with DCD showed reduced nitrate content and low nitrification activity after 3 and 7 days of incubation. This was mirrored by a 70 % reduction in potential nitrification activity compared to a nitrogen-only control. None of the incubation treatments affected nontarget microbial counts as estimated by 16S rRNA gene copy numbers, however, the high dose of MHPP significantly reduced the abundance of ammonia-oxidizing bacteria and archaea. Conclusions These findings suggest that MHPP is capable of suppressing nitrification in soil, possibly by reducing the population size and activity of ammoniaoxidizing microorganisms.

The relevance of precision agriculture produced a growth of the related literature over the years. However, a structured analysis of the published material is still missing. Thus, this study attempts to analyze the global scientific output of precision agriculture researches published during the period 2000–2016. By using a science mapping approach, mainly based on the application of network analysis tools, it was possible to investigate pivotal aspects of this research field such as publication trends, research topics and their geographical distribution. Using the Scopus database 17,756 scientific publications were retrieved from the chosen period. The number of publications increased after 2006, highlighting the vibrancy of the field. By authoring 35% of the publications, U.S.A. and China were the most active knowledge producer countries. Moreover, the generation of time resolved maps allowed us to identify agriculture engineering, computer science and agriculture studies as three main research areas characterizing precision agriculture panorama. The paper discusses the distribution of these topics at global level, among European countries and in Italy. Overall, this analysis represents the first holistic view of precision agriculture research providing valuable information for farmers, policy makers and researchers.

Nitrification is the microbial conversion of reduced forms of nitrogen (N) to nitrate (NO3−), and in fertilized soils it can lead to substantial N losses via NO3− leaching or nitrous oxide (N2O) production. To limit such problems, synthetic nitrification inhibitors have been applied but their performance differs between soils. In recent years, there has been an increasing interest in the occurrence of biological nitrification inhibition (BNI), a natural phenomenon according to which certain plants can inhibit nitrification through the release of active compounds in root exudates. Here, we synthesize the current state of research but also unravel knowledge gaps in the field. The nitrification process is discussed considering recent discoveries in genomics, biochemistry and ecology of nitrifiers. Secondly, we focus on the ‘where’ and ‘how’ of BNI. The N transformations and their interconnections as they occur in, and are affected by, the rhizosphere, are also discussed. The NH4+ and NO3− retention pathways alternative to BNI are reviewed as well. We also provide hypotheses on how plant compounds with putative BNI ability can reach their targets inside the cell and inhibit ammonia oxidation. Finally, we discuss a set of techniques that can be successfully applied to solve unresearched questions in BNI studies.

Screening numerous genotypes for nitrification inhibition activity provides first evidence that root exudates of rice can slow the nitrification of ammonium to nitrate in soil. This offers promising possibilities for exploiting molecular and genetic tools to improve nitrogen use efficiency by inhibiting wasteful nitrification. Abstract Background and aims Nitrification is an important process in soil--plant systems for providing plant-available nitrate (NO3−). However, NO3− is less stable in soils compared with ammonium (NH4+) and is more easily lost through leaching, runoff or denitrification. This study tested whether biological nitrification inhibition (BNI) activity is present in the root exudates of rice (Oryza sativa) and also the extent of variation between different genotypes. Methodology The BNI activity of root exudates was estimated by a bioluminescence assay using a recombinant Nitrosomonas europaea strain. Afterwards, the effect of a single application of concentrated root exudates and that of exudates deposited in the rhizosphere soil was tested on BNI using soil incubation. Soil was added with (NH4)2SO4 and water to reach 60 % of the water-holding capacity and incubated at 30 °C for different periods. Amounts of NH4+ and NO3− were determined using a continuous-flow auto-analyser. Principal results In an initial screening experiment, BNI activity in the exudates of 36 different rice genotypes was evaluated using a bioassay based on a recombinant Nitrosomonas strain. Significant genotypic variation was detected with the upland cultivar IAC25 demonstrating consistently high BNI activity, while modern lowland varieties like Nipponbare or IR64 exhibited lower activity. Subsequent experiments ruled out the possibility that BNI activity is simply due to non-specific (solute) leakage from roots. Soil incubation studies with concentrated root exudates of IAC25 showed significant reductions in NO3− formation. This effect was confirmed by detecting lower NO3− levels in incubation experiments using rhizosphere soil obtained from IAC25. Conclusions Our results provide first evidence that root exudates of rice can reduce nitrification rates in soil. Having shown this for a model crop, rice, offers possibilities for further exploitation of this phenomenon through molecular and genetic tools.

Olive oil contains different biologically active polyphenols, among which oleacein, the most abundant secoiridoid, has recently emerged for its beneficial properties in various disease contexts. By using in vitro models of human multiple myeloma (MM), we here investigated the anti-tumor potential of oleacein and the underlying bio-molecular sequelae. Within a low micromolar range, oleacein reduced the viability of MM primary samples and cell lines even in the presence of bone marrow stromal cells (BMSCs), while sparing healthy peripheral blood mononuclear cells. We also demonstrated that oleacein inhibited MM cell clonogenicity, prompted cell cycle blockade and triggered apoptosis. We evaluated the epigenetic impact of oleacein on MM cells, and observed dose-dependent accumulation of both acetylated histones and α-tubulin, along with down-regulation of several class I/II histone deacetylases (HDACs) both at the mRNA and protein level, providing evidence of the HDAC inhibitory activity of this compound; conversely, no effect on global DNA methylation was found. Mechanistically, HDACs inhibition by oleacein was associated with down-regulation of Sp1, the major transactivator of HDACs promoter, via Caspase 8 activation. Of potential translational significance, oleacein synergistically enhanced the in vitro anti-MM activity of the proteasome inhibitor carfilzomib. Altogether, these results indicate that oleacein is endowed with HDAC inhibitory properties, which associate with significant anti-MM activity both as single agent or in combination with carfilzomib. These findings may pave the way to novel potential anti-MM epi-therapeutic approaches based on natural agents.

Introduction: Coronavirus disease 2019 (COVID-19) is a significant and novel cause of acute respiratory distress syndrome (ARDS). During the COVID-19 pandemic, there has been an increase in the incidence of cases involving pneumothorax and pneumomediastinum. However, the risk factors associated with poor outcomes in these patients remain unclear. Methods: This observational study collected clinical and imaging data from COVID-19 patients with PTX and/or PNM across five tertiary hospitals in central Italy between 1 March 2020 and 1 March 2022. This study also calculated the incidence of PTX and PNM and utilized multivariable regression analysis and Kaplan–Meier curve analysis to identify predictor factors for 28-day mortality and 3-day orotracheal intubation after PTX/PNM. This study also considered the impact of the three main variants of concern (VoCs) (alfa, delta, and omicron) circulating during the study period. Results: During the study period, a total of 11,938 patients with COVID-19 were admitted. This study found several factors independently associated with a higher risk of death in COVID-19 patients within 28 days of pulmonary barotrauma. These factors included a SOFA score ≥ 4 (OR 3.22, p = 0.013), vasopressor/inotropic therapy (OR 11.8, p < 0.001), hypercapnia (OR 2.72, p = 0.021), PaO2/FiO2 ratio < 150 mmHg (OR 10.9, p < 0.001), and cardiovascular diseases (OR 7.9, p < 0.001). This study also found that a SOFA score ≥ 4 (OR 3.10, p = 0.015), PCO2 > 45 mmHg (OR 6.0, p = 0.003), and P/F ratio < 150 mmHg (OR 2.9, p < 0.042) were factors independently associated with a higher risk of orotracheal intubation (OTI) within 3 days from PTX/PNM in patients with non-invasive mechanical ventilation. SARS-CoV-2 VoCs were not associated with 28-day mortality or the risk of OTI. The estimated cumulative probability of OTI in patients after pneumothorax was 44.0% on the first day, 67.8% on the second day, and 68.9% on the third day, according to univariable survival analysis. In patients who had pneumomediastinum only, the estimated cumulative probability of OTI was 37.5%, 46.7%, and 57.7% on the first, second, and third days, respectively. The overall incidence of PTX/PNM among hospitalized COVID-19 patients was 1.42%, which increased up to 4.1% in patients receiving invasive mechanical ventilation. Conclusions: This study suggests that a high SOFA score (≥4), the need for vasopressor/inotropic therapy, hypercapnia, and PaO2/FiO2 ratio < 150 mmHg in COVID-19 patients with pulmonary barotrauma are associated with higher rates of intubation, ICU admission, and mortality. Identifying these risk factors early on can help healthcare providers anticipate and manage these patients more effectively and provide timely interventions with appropriate intensive care, ultimately improving their outcomes.