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result(s) for
"Negri, Barbara"
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Genome-Wide Association Study for Root Morphology and Phosphorus Acquisition Efficiency in Diverse Maize Panels
by
Negri, Barbara França
,
Magalhaes, Jurandir Vieira
,
Buckler, Edward S.
in
Agricultural production
,
Biomass
,
Corn
2023
Maximizing soil exploration through modifications of the root system is a strategy for plants to overcome phosphorus (P) deficiency. Genome-wide association with 561 tropical maize inbred lines from Embrapa and DTMA panels was undertaken for root morphology and P acquisition traits under low- and high-P concentrations, with 353,540 SNPs. P supply modified root morphology traits, biomass and P content in the global maize panel, but root length and root surface area changed differentially in Embrapa and DTMA panels. This suggests that different root plasticity mechanisms exist for maize adaptation to low-P conditions. A total of 87 SNPs were associated to phenotypic traits in both P conditions at −log10(p-value) ≥ 5, whereas only seven SNPs reached the Bonferroni significance. Among these SNPs, S9_137746077, which is located upstream of the gene GRMZM2G378852 that encodes a MAPKKK protein kinase, was significantly associated with total seedling dry weight, with the same allele increasing root length and root surface area under P deficiency. The C allele of S8_88600375, mapped within GRMZM2G044531 that encodes an AGC kinase, significantly enhanced root length under low P, positively affecting root surface area and seedling weight. The broad genetic diversity evaluated in this panel suggests that candidate genes and favorable alleles could be exploited to improve P efficiency in maize breeding programs of Africa and Latin America.
Journal Article
Gravity evidence for a heterogeneous crust of Mercury
2023
We modeled gravity data to explore Mercury’s internal structure and show the presence of crustal heterogeneities in density. We first evaluated the lithospheric flexure occurring in the spherical harmonic degree range 5–80, according to the flexural isostatic response curve. We thus estimated a mean elastic lithosphere thickness of about 30
±
10 km and modeled the crust-mantle interface, which varies from 19 to 42 km depth, according to a flexural compensation model. The isostatic gravity anomalies were then obtained as the residual field with respect to the contributions from topography and lithospheric flexure. Isostatic anomalies are mainly related to density variations in the crust: gravity highs mostly correspond to large-impact basins suggesting intra-crustal magmatic intrusions as the main origin of these anomalies. Isostatic gravity lows prevail, instead, above intercrater plains and may represent the signature of a heavily fractured crust.
Journal Article
Mpp23Aa/Xpp37Aa Insecticidal Proteins from Bacillus thuringiensis (Bacillales: Bacillaceae) Are Highly Toxic to Anthonomus grandis (Coleoptera: Curculionidae) Larvae
by
Escriche, Baltasar
,
de Oliveira, Jéssica A.
,
Hernández-Martínez, Patricia
in
Animals
,
Anthonomus grandis
,
Artificial diets
2023
The beetle Anthonomus grandis Boheman, 1843, is the main cotton pest, causing enormous losses in cotton. The breeding of genetically modified plants with A. grandis resistance is seen as an important control strategy. However, the identification of molecules with high toxicity to this insect remains a challenge. The susceptibility of A. grandis larvae to proteins (Cry1Ba, Cry7Ab, and Mpp23Aa/Xpp37Aa) from Bacillus thuringiensis Berliner, 1915, with toxicity reported against Coleopteran, has been evaluated. The ingestion of different protein concentrations (which were incorporated into an artificial diet) by the larvae was tested in the laboratory, and mortality was evaluated after one week. All Cry proteins tested exhibited higher toxicity than that the untreated artificial diet. These Cry proteins showed similar results to the control Cry1Ac, with low toxicity to A. grandis, since it killed less than 50% of larvae, even at the highest concentration applied (100 μg·g−1). Mpp/Xpp proteins provided the highest toxicity with a 0.18 μg·g−1 value for the 50% lethal concentration. Importantly, this parameter is the lowest ever reported for this insect species tested with B. thuringiensis proteins. This result highlights the potential of Mpp23Aa/Xpp37Aa for the development of a biotechnological tool aiming at the field control of A. grandis.
Journal Article
Current state and perspectives of Space Weather science in Italy
by
Bemporad, Alessandro
,
Messerotti, Mauro
,
Polenta, Gianluca
in
Atmosphere
,
Charged particles
,
Climate models
2020
Italian teams have been involved many times in Space Weather observational campaigns from space and from the ground, contributing in the advancing of our knowledge on the properties and evolution of the related phenomena. Numerous Space Weather forecasting and now-casting modeling efforts have resulted in a remarkable add-on to the overall progress in the field, at both national and international level. The Italian Space Agency has participated several times in space missions with science objectives related to Space Weather; indeed, an important field for the Italian scientific and industrial communities interested in Heliophysics and Space Weather, is the development of new instrumentation for future space missions. In this paper, we present a brief state-of-the-art in Space Weather science in Italy and we discuss some ideas on a long-term plan for the support of future scientific research in the related disciplines. In the context of the current roadmap, the Italian Space Agency aims to assess the possibility to develop a national scientific Space Weather data centre to encourage synergies between different science teams with interest in the field and to motivate innovation and new mission concept development. Alongside with the proposed recommendations, we also discuss how the Italian expertise could complement international efforts in a wider international Space Weather context.
Journal Article
Multiple interval QTL mapping and searching for PSTOL1 homologs associated with root morphology, biomass accumulation and phosphorus content in maize seedlings under low-P
by
Azevedo, Gabriel C
,
Hufnagel, Bárbara
,
Guimaraes, Claudia T
in
Accumulation
,
Agriculture
,
alleles
2015
Background
Modifications in root morphology are important strategies to maximize soil exploitation under phosphorus starvation in plants. Here, we used two multiple interval models to map QTLs related to root traits, biomass accumulation and P content in a maize RIL population cultivated in nutrient solution. In addition, we searched for putative maize homologs to
PSTOL1
, a gene responsible to enhance early root growth, P uptake and grain yield in rice and sorghum.
Results
Based on path analysis, root surface area was the root morphology component that most strongly contributed to total dry weight and to P content in maize seedling under low-P availability. Multiple interval mapping models for single (MIM) and multiple traits (MT-MIM) were combined and revealed 13 genomic regions significantly associated with the target traits in a complementary way. The phenotypic variances explained by all QTLs and their epistatic interactions using MT-MIM (23.4 to 35.5 %) were higher than in previous studies, and presented superior statistical power. Some of these QTLs were coincident with QTLs for root morphology traits and grain yield previously mapped, whereas others harbored
ZmPSTOL
candidate genes, which shared more than 55 % of amino acid sequence identity and a conserved serine/threonine kinase domain with
OsPSTOL1
. Additionally, four
ZmPSTOL
candidate genes co-localized with QTLs for root morphology, biomass accumulation and/or P content were preferentially expressed in roots of the parental lines that contributed the alleles enhancing the respective phenotypes.
Conclusions
QTL mapping strategies adopted in this study revealed complementary results for single and multiple traits with high accuracy. Some QTLs, mainly the ones that were also associated with yield performance in other studies, can be good targets for marker-assisted selection to improve P-use efficiency in maize. Based on the co-localization with QTLs, the protein domain conservation and the coincidence of gene expression, we selected novel maize genes as putative homologs to
PSTOL1
that will require further validation studies.
Journal Article
Duplicate and Conquer: Multiple Homologs of PHOSPHORUS-STARVATION TOLERANCE1 Enhance Phosphorus Acquisition and Sorghum Performance on Low-Phosphorus Soils
2014
Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) protein kinase, PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates that multiple SbPSTOL1 genes have a more general role in the root system, not only enhancing root morphology traits but also changing root system architecture, which leads to grain yield gain under low-P availability in the soil.
Journal Article
Bacillus subtilis as growth-promoting rhizobacteria co-inoculated on Bradyrhizobium-treated soybean seeds in the planting furrow
by
Oliveira, Jéssica Alves de
,
Galbieri, Rafael
,
Negri, Bárbara França
in
Acetic acid
,
Agricultural production
,
AGRICULTURE, MULTIDISCIPLINARY
2023
According to the Brazilian Soybean Strategic Committee (CESB), the yield potential could exceed 5,000 kg ha-1 in certain regions of the country (Battisti et al., 2018). Auxins, of which the most important is indole-3-acetic acid (IAA), are key phytohonnones in the regulation of root system architecture, for controlling primary root elongation and lateral root fonnation (Poveda & González-Andrés. 2021). The objectives of this study were to characterize the B. subtilis isolate - IMA Bs/170005 and to evaluate how co-inoculating it in the soybean planting furrow with Bradyrhizobium-treated seed affects plant growth and yield under tropical climate conditions. The cell culture was centrifuged at 10000 rpm for 15 min, and 1 mL of the supernatant was mixed with 1 mL of Salkowski solution, followed by incubation at room temperature protected from light for 30 min.
Journal Article
High-Density Pixel Imaging Sensor Readout Electronics for Space Applications: A Design Overview
by
Orsini, Leonardo
,
Bachetti, Matteo
,
Pieraccini, Stefano
in
Application-specific integrated circuits
,
Communication
,
Custom integrated circuits
2023
With the specialization of VLSI ASICs for front-end signal processing electronics, the customization of the control back-end electronics (BEE) has become critical to fully deploy the ASIC performance. In the context of space operations, with typical constraints on power and reliability, the design and qualification of such integrated systems present significant challenges. In this paper, we review the design and performance of the BEE systems after two years of operations in low Earth orbit (LEO); these systems read out the custom ASICs inside the gas pixel detectors, which are located at the heart of the imaging X-ray polarimetry explorer (IXPE), a NASA-ASI small explorer mission designed to measure X-ray polarization in the 2–8 keV energy range.
Journal Article
Calibration of the IXPE focal plane X-ray polarimeters to polarized radiation
by
Orsini, Leonardo
,
Bachetti, Matteo
,
Pieraccini, Stefano
in
Calibration
,
Energy bands
,
Focal plane
2022
IXPE (Imaging X-ray Polarimetry Explorer) is a NASA Small Explorer mission -- in partnership with the Italian Space Agency (ASI) -- dedicated to X-ray polarimetry in the 2--8 keV energy band. The IXPE telescope comprises three grazing incidence mirror modules coupled to three detector units hosting each one a Gas Pixel Detector (GPD), a gas detector that allows measuring the polarization degree by using the photoelectric effect. A wide and accurate ground calibration was carried out on the IXPE Detector Units (DUs) at INAF-IAPS, in Italy, where a dedicated facility was set-up at this aim. In this paper, we present the results obtained from this calibration campaign to study the IXPE focal plane detector response to polarized radiation. In particular, we report on the modulation factor, which is the main parameter to estimate the sensitivity of a polarimeter.
The Imaging X-Ray Polarimetry Explorer (IXPE): Technical Overview IV
by
Orsini, Leonardo
,
DAmico, Fabio
,
Deininger, William D
in
Polarimetry
,
Space missions
,
Spacecraft attitude control
2021
Scheduled to launch in late 2021,the Imaging X-ray Polarimetry Explorer (IXPE) is a Small Explorer Mission designed to open up a new window of investigation --X-ray polarimetry. The IXPE observatory features 3 identical telescopes each consisting of a mirror module assembly with a polarization-sensitive imaging x-ray detector at its focus. An extending boom, deployed on orbit, provides the necessary 4 m focal length. The payload sits atop a 3-axis stabilized spacecraft which, among other things, provides power, attitude determination and control, commanding, and telemetry to the ground. During its 2-year baseline mission, IXPE will conduct precise polarimetry for samples of multiple categories of x-ray sources, with follow-on observations of selected targets. IXPE is a partnership between NASA and the Italian Space Agency (ASI).
Conference Proceeding