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Objectives This study aimed to compare the cleansing efficacy of the Oral-B ® iO™ electric toothbrush incorporating oscillating-rotating technology with microvibrations - with a traditional oscillating-rotating toothbrush. Materials and methods Thirty adult participants were randomly assigned to use the iO™ electric toothbrush with the brush head iO™ Ultimate Clean or the traditional oscillating-rotating toothbrush Oral-B ® Genius ® with the Cross-Action brush head. Oral hygiene indices (Rustogi Modified Navy Plaque Index and Gingival Bleeding Index) were assessed before and after 28 days of home use of the assigned product. Participants were instructed to refrain from interdental hygiene during the study period. After a 2-week washout period, the clinical investigation was repeated in a crossover design. Results All 30 participants completed the study with no dropouts. After 28 days of use, the iO™ showed statistically significantly lower plaque levels than the conventional oscillating-rotating toothbrush (25.09% vs. 30.60%, p  = 0.029). This difference was particularly noticeable in marginal and approximal areas. There were no significant distinctions in gingival bleeding indices. Conclusions The Oral-B ® iO™ electric toothbrush displayed enhanced plaque removal efficiency compared to a conventional oscillating-rotating technology. Clinical relevance This study highlights the potential benefits of advanced toothbrush technologies for plaque reduction and encourages further research.

Transmission spectroscopy probes exoplanet atmospheres via the wavelength dependence of transit depths, but stellar contamination from magnetic activity can significantly bias these measurements. Activity-induced changes in the chromatic apparent stellar radius represent a major challenge for atmospheric characterisation. As surface distributions of magnetic features are generally unknown for stars other than the Sun, we adopt the Sun as a benchmark to study how the chromatic effect depends on the distribution of spots and faculae. Using spot and facular masks derived from SDO/HMI magnetograms and intensitygrams, combined with the SATIRE model, we compute the chromatic dependence of the Sun's apparent radius. We test different methods of convolving surface coverage with spectra the identify physical drivers of the effect. We find that simplified approaches, which neglect the CLV, underestimate the apparent radius, particularly for faculae, whose surface coverage dominates at near-solar activity levels. Proper treatment of facular CLV is therefore essential. The activity-induced variation between solar minimum and maximum reaches around 40 ppm for a Jupiter-like transit, exceeding JWST's expected 10 ppm noise floor, while remaining at around 0.4 ppm for an Earth-like transit.

The plethora of photometric data collected by the Kepler space telescope has promoted the detection of tens of thousands of stellar rotation periods. However, these periods are not found to an equal extent among different spectral types. Interestingly, early G-type stars with near-solar rotation periods are strongly underrepresented among those stars with known rotation periods. In this study we investigate whether the small number of such stars can be explained by difficulties in the period determination from photometric time series. For that purpose, we generate model light curves of early G-type stars with solar rotation periods for different inclination angles, metallicities and (magnitude-dependent) noise levels. We find that the detectability is determined by the predominant type of activity (i.e. spot or faculae domination) on the surface, which defines the degree of irregularity of the light curve, and further depends on the level of photometric noise. These two effects significantly complicate the period detection and explain the lack of solar-like stars with known near-solar rotation periods. We conclude that the rotation periods of the majority of solar-like stars with near-solar rotation periods remain undetected to date. Finally, we promote the use of new techniques to recover more periods of near-solar rotators.

The unprecedented precision of broadband stellar photometry achieved with the planet-hunting missions CoRoT and \\textit{Kepler} initiated a new era in examining the magnetically-driven brightness variations of hundreds of thousands of stars. Such brightness variations are well studied and understood for the Sun. The plethora of data allows to accurately compare solar and stellar brightness variations. An intriguing question is whether the observed trends in the stellar photometric variability (e.g. the dependence of the variability on the stellar rotation period) can be explained by utilising the solar paradigm, in particular the physical concepts of brightness variations learnt from the Sun. The goal of this work is to find out, through comparison of observational and simulated data, if any physical concepts of solar brightness variability have to be altered to reproduce the distribution of Sun-like stars variabilities.

Background Inflammation and oxidative stress can contribute to the development and progression of heart failure. This study aimed to investigate the association between inflammatory and oxidative stress markers in dogs with congestive heart failure (CHF). Associations between the disease severity marker N-terminal pro-B-type natriuretic peptide (NT-proBNP) and markers of inflammation and oxidative stress were also determined. Results Thirty-seven dogs with cardiovascular diseases (dilated cardiomyopathy, DCM (16 dogs), myxomatous mitral valve disease, MMVD (21 dogs)) and ten healthy dogs were included in this prospective study. The patients were further divided into groups with (26) and without CHF (11). We found a significantly higher serum concentration of C-reactive protein ( P  = 0.012), white blood cell ( P  = 0.001), neutrophil ( P  = 0.001) and monocyte counts ( P  = 0.001) in patients with CHF compared to control dogs. The concentration of tumor necrosis factor-alpha (TNF-α) was significantly higher in patients with CHF compared to patients without CHF ( P  = 0.030). No significant difference was found in most of the measured parameters between MMVD and DCM patients, except for glutathione peroxidase (GPX) and NT-proBNP. In patients with CHF, TNF-α correlated positively with malondialdehyde ( P  = 0.014, r  = 0.474) and negatively with GPX ( P  = 0.026, r  = − 0.453), and interleukin-6 correlated negatively with GPX ( P  = 0.046, r  = − 0.412). NT-proBNP correlated positively with malondialdehyde ( P  = 0.011, r  = 0.493). In patients without CHF none of the inflammatory and oxidative stress markers correlated significantly. Furthermore, in the group of all cardiac patients, GPX activity significantly negatively correlated with NT-proBNP ( P  = 0.050, r  = − 0.339) and several markers of inflammation, including TNF-α ( P  = 0.010, r  = − 0.436), interleukin-6 ( P  = 0.026, r  = − 0.382), white blood cell ( P  = 0.032, r  = − 0.369), neutrophil ( P  = 0.027, r  = − 0.379) and monocyte counts ( P  = 0.024, r  = − 0.386). Conclusion Inflammatory and oxidative stress markers are linked in canine CHF patients, but not in patients without CHF. These results suggest complex cross communication between the two biological pathways in advanced stages of CHF.

The aim of this study was to evaluate the efficacy of metronomic chemotherapy in the palliative treatment of various malignant oral tumors in dogs. Our focus was to determine the effect of treatment on local disease control and to assess the tolerability and safety of the treatment in dogs with various oral malignancies. Metronomic chemotherapy with cyclophosphamide was used to treat 12 dogs and was combined with non-steroidal anti-inflammatory drugs in 6/12 (50%) of dogs. A clinical benefit was observed in 6/12 (50%) patients 1 month and in 4/12 (33%) 3 months after treatment initiation. The median survival time of the dogs was 155 days (range 21–529 days). At the end of the observation period, the disease had progressed in 10/12 (83.3%) of the patients. Sterile hemorrhagic cystitis was the most commonly reported side effect of treatment, occurring in 4/12 (33.3%) dogs. The results of our study suggest that metronomic chemotherapy with cyclophosphamide can be, in a subset of dogs, beneficial in the palliation of malignant oral tumors.

Electrochemotherapy (ECT) in combination with the gene electrotransfer of interleukin 12 (IL-12 GET) has been successfully used in veterinary medicine for the treatment of mast cell tumours (MCT), but the biomarkers that could predict response to this treatment have not yet been investigated. The aim of this study was to determine the plasma nucleosome and serum ferritin concentrations, as well as the lactate dehydrogenase (LDH) activity, in the serum of treated patients before and one and six months after treatment to evaluate their utility as potential biomarkers that could predict response to the combined treatment. The study was conducted in 48 patients with a total of 86 MCTs that we treated with the combined treatment. The blood samples used for analysing the potential predictive biomarkers were taken before treatment and one and six months after treatment, when the response to treatment was also assessed. The Nu. Q® Vet Cancer Test, the Canine Ferritin ELISA Kit, and the RX Daytona+ automated biochemical analyser were used to analyse the blood samples. The results showed that the plasma nucleosome concentration (before treatment (BT): 32.84 ng/mL (median); one month after treatment (1 M AT): 58.89 ng/mL (median); p = 0.010) and serum LDH activity (BT: 59.75 U/L (median); 1 M AT: 107.5 U/L (median); p = 0.012) increased significantly one month after treatment and that the increase correlated significantly with the presence of a more pronounced local reaction (necrosis, swelling, etc.) at that time point for both markers (nucleosome: BT (necrosis): 21.61 ng/mL (median); 1 M AT (necrosis): 69.92 ng/mL (median), p = 0.030; LDH: BT (necrosis): 54.75 U/L (median); 1 M AT (necrosis): 100.3 U/L (median), p = 0.048). Therefore, both the plasma nucleosome concentration and serum LDH activity could serve as early indicators of the effect of the treatment. In this context, the serum ferritin concentration showed no significant predictive potential for treatment response (p > 0.999 for all comparisons). In conclusion, this study provides some new and important observations on the use of predictive biomarkers in veterinary oncology. Furthermore, it emphasises the need for the continued identification and validation of potential predictive biomarkers in dogs with MCT and other malignancies undergoing ECT treatment in combination with IL-12 GET to ultimately improve treatment outcomes.

Localized surface plasmon resonance (LSPR) is the cause of the photo-thermal effect observed in topological insulator (TI) bismuth selenide (Bi2Se3) nanoparticles. These plasmonic properties, which are thought to be caused by its particular topological surface state (TSS), make the material interesting for application in the field of medical diagnosis and therapy. However, to be applied, the nanoparticles have to be coated with a protective surface layer, which prevents agglomeration and dissolution in the physiological medium. In this work, we investigated the possibility of using silica as a biocompatible coating for Bi2Se3 nanoparticles, instead of the commonly used ethylene-glycol, which, as is presented in this work, is not biocompatible and alters/masks the optical properties of TI. We successfully prepared Bi2Se3 nanoparticles coated with different silica layer thicknesses. Such nanoparticles, except those with a thick, ≈200 nm silica layer, retained their optical properties. Compared to ethylene-glycol coated nanoparticles, these silica coated nanoparticles displayed an improved photo-thermal conversion, which increased with the increasing thickness of the silica layer. To reach the desired temperatures, a 10–100 times lower concentration of photo-thermal nanoparticles was needed. In vitro experiments on erythrocytes and HeLa cells showed that, unlike ethylene glycol coated nanoparticles, silica coated nanoparticles are biocompatible.

Electroporation is a method of inducing an increase in permeability of the cell membrane through the application of an electric field and can be used as a delivery method for introducing molecules of interest (e.g., chemotherapeutics or plasmid DNA) into cells. Electroporation-based treatments (i.e., electrochemotherapy, gene electrotransfer, and their combinations) have been shown to be safe and effective in veterinary oncology, but they are currently mostly recommended for the treatment of those solid tumors for which clients have declined surgery and/or radiotherapy. Published data show that electroporation-based treatments are also safe, simple, fast and cost-effective treatment alternatives for selected oral and maxillofacial tumors, especially small squamous cell carcinoma and malignant melanoma tumors not involving the bone in dogs. In these patients, a good local response to treatment is expected to result in increased survival time with good quality of life. Despite emerging evidence of the clinical efficacy of electroporation-based treatments for oral and maxillofacial tumors, further investigation is needed to optimize treatment protocols, improve clinical data reporting and better understand the mechanisms of patients' response to the treatment.

Objectives The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. Methods Fifteen patients in mixed dentition treated with a bonded palatal expander were enrolled in this longitudinal study. Saliva samples were taken before the therapy, as well as in 2 weeks and 3, 6, 9, and 12 months after the beginning of the therapy. In each sample, the levels of MRP-8/14 were determined by ELISA and the presence of 11 bacteria was detected by PCR followed by DNA-DNA hybridization. Results Salivary concentration of MRP-8/14 and the amount of Tannerella forsythia , Treponema denticola , and Eikenella corrodens were significantly increased during treatment with bonded maxillary expander. These changes were transient and the maximal levels of MRP-8/14 and periodontitis-associated pathogens were observed 6–9 months after the beginning of the therapy. Conclusion Therapy with bonded maxillary results in higher MRP-8/14 levels and increased prevalence of some periodontitis-associated bacteria, namely T. forsythia , T. denticola , and E. corrodens . The results suggest the detection of salivary MRP-8/14 levels may be a potential tool to reflect the oral health status in children with fixed orthodontic treatment. Clinical relevance Our data suggest that the treatment with bonded maxillary expander might influence the oral health status and should be accompanied by the careful control of the oral health during the therapy.