Explore the vast range of titles available.

\"Battling both inner and external demons, Stephanie must learn to balance school and crime-fighting or face the wrath of Barbara Gordon! With guest appearences from Batman and Robin and villains like Man-Bat and Clayface, Batgirl must step up to the mantle! Batgirl must battle the Calculator and stop his plan to unleash a nanovirus upon the citizens of Gotham City that will turn them into mindless techno-zombies, enter the FLOOD!\"-- Provided by publisher.

By adopting a person-centered approach, this research explored emotional labor latent profiles based on employees’ levels of surface acting and deep acting. Further, this study examined the role of workplace mistreatment from different sources (customer incivility, coworker incivility, abusive supervision, and organizational dehumanization) in the prediction of profile membership and the associations between these profiles and several outcomes (job satisfaction, turnover intentions, emotional exhaustion, and affective commitment). Five profiles of emotional laborers were identified (surface actors, regulators, low actors, non-actors, and deep actors). Customer incivility, coworker incivility, and organizational dehumanization but not abusive supervision were found to be associated with profile membership. Particularly, employees who perceived high levels of organizational dehumanization had a higher likelihood to be identified as surface actors. Overall, positive outcomes were associated with deep actors, while surface actors were more likely to be related to negative outcomes. Our findings provide further support for the advantages of adopting a person-centered perspective to the study of emotional labor.

Fluorescent proteins are indispensable molecular tools for visualizing biological structures and processes, but their limited photostability restricts the duration of dynamic imaging experiments. Yellow fluorescent proteins (YFPs), in particular, photobleach rapidly. Here, we introduce mGold2s and mGold2t, YFPs with up to 25-fold greater photostability than mVenus and mCitrine, two commonly used YFPs, while maintaining comparable brightness. These variants were identified using a high-throughput pooled single-cell platform, simultaneously screening for high brightness and photostability. Compared with our previous benchmark, mGold, the mGold2 variants display a ~4-fold increase in photostability without sacrificing brightness. mGold2s and mGold2t extend imaging durations across diverse modalities, including widefield, total internal reflection fluorescence (TIRF), super-resolution, single-molecule, and laser-scanning confocal microscopy. When incorporated into fluorescence resonance energy transfer (FRET)-based biosensors, the proposed YFPs enable more reliable, prolonged imaging of dynamic cellular processes. Overall, the enhanced photostability of mGold2s and mGold2t enables high-sensitivity imaging of subcellular structures and cellular activity over extended periods, broadening the scope and precision of biological imaging. Yellow fluorescent proteins (YFPs) photobleach rapidly, restricting microscopy experiments. Here, the authors report mGold2s and mGold2t, YFPs that extend imaging durations up to 25 times longer than standard probes without sacrificing brightness.

Colorectal cancer is the third most commonly diagnosed cancer in the world that affects both men and women. Approximately 40% of colorectal cancer patients exhibit cognitive impairment in executive functions including verbal learning, verbal memory, and information processing that is independent of chemotherapy. However, little information is currently available regarding the neural mechanisms underlying colorectal cancer-related cognitive decline (CRCD). In this study, we utilized highly sensitive 7 Tesla magnetic resonance imaging methods combined with standardized cognitive assessments to investigate the changes in brain local functional connectivity in early-stage colorectal cancer survivors compared to healthy controls. We observed that early-stage colorectal cancer survivors exhibited increased regional homogeneity (ReHo) in the left hippocampus, parahippocampal gyrus, and inferior temporal gyrus, along with decreased ReHo in the left inferior frontal gyrus, which were associated with reduced verbal memory performance compared to healthy controls. Furthermore, survivors exhibited significantly weaker inter-regional functional connectivity, suggesting a potential disruption in coordination among regions critical for verbal memory. Collectively, these findings indicate a maladaptive mechanism in the medial temporal lobes that is associated with declines in verbal memory processes among colorectal cancer survivors. ReHo analysis was found to be a valuable tool for characterizing the neurophysiological basis of colorectal CRCD and presents the medial temporal lobe as a promising target for therapeutic interventions.

Background Adolescence is a critical period for developing behaviors that affect lifelong cardiovascular health. While physical activity improves cardiovascular outcomes, excessive recreational screen time is linked to negative cardiovascular indicators. This study examined associations between screen time and physical activity with cardiovascular risk factors in a large, diverse sample of early adolescents. Methods This study included 4,443 adolescents from the Adolescent Brain Cognitive Development (ABCD) Study collected at Year 2 (2018–2020) and Year 4 (2020–2022). Screen time was self-reported and categorized as low (0–4), medium (> 4–8), or high (> 8) hours/day. Physical activity was measured via Fitbit step count over 3 weeks and categorized as low (1,000–6,000), medium (> 6,000–12,000), and high (> 12,000) steps/day. Outcomes included blood pressure percentile and hypertensive-range blood pressure, which were available for the full sample, while total cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol, hemoglobin A1c, and testing consistent with diabetes were available in a subset (one-third) at Year 4. Adjusted linear and Poisson regression models examined joint associations of screen time and step count with cardiovascular health outcomes. Results Adolescents averaged 6.1 (± 5.1) hours/day of screen use and 9,280 (± 3,279) steps/day. Higher screen time and lower step count showed dose-response associations with higher diastolic blood pressure percentile (all p  < 0.01). Compared to reference categories, high screen time (> 8 h/day) was associated with a 4.25-point increase in diastolic blood pressure percentile (95% confidence interval [CI] 1.85 to 6.65) and low step count (1,000–6,000 steps/day) was associated with a 7.15-point increase (95% CI 4.82 to 9.48). Both high screen time (adjusted risk ratio [ARR] 1.88, 95% CI 1.42 to 2.56) and low step count (ARR 2.35, 95% CI 1.88 to 2.94) were associated with increased risk of hypertensive-range blood pressure. Low step count was associated with -3.72 mg/dL lower HDL cholesterol (95% CI -6.52 to -2.92) and increased risk of low HDL cholesterol (ARR 2.05, 95% CI 1.79 to 2.36). Conclusions Higher screen time and lower physical activity were associated with poorer cardiovascular health, supporting guidelines and early interventions promoting increased physical activity and reduced screen time.

This study aimed to examine the mechanisms underlying the negative relationship between the feeling of being dehumanized by the organization and employees’ job satisfaction. More precisely, we argue that emotional labor (i.e., surface acting) and core self-evaluations act as mediators in this relationship. A total of 326 employees participated in our study. Firstly, the results showed that, independently of one another, both surface acting and core self-evaluations partially mediated the relationship between organizational dehumanization and job satisfaction. Secondly, surface acting and core self-evaluations were found to have serial mediation effects in this relationship. Accordingly, experiencing dehumanization from the organization leads employees to perform more surface acting with deleterious consequences for their core self-evaluations and finally their job satisfaction.

The Tn3 family is a widespread group of replicative transposons that are notorious for their contribution to the dissemination of antibiotic resistance and the emergence of multiresistant pathogens worldwide. The TnpA transposase of these elements catalyzes DNA breakage and rejoining reactions required for transposition. It also is responsible for target immunity, a phenomenon that prevents multiple insertions of the transposon into the same genomic region. However, the molecular mechanisms whereby TnpA acts in both processes remain unknown. Here, we have developed sensitive biochemical assays for the TnpA transposase of the Tn3-family transposon Tn4430 and used these assays to characterize previously isolated TnpA mutants that are selectively affected in immunity. Compared with wild-type TnpA, these mutants exhibit deregulated activities. They spontaneously assemble a unique asymmetric synaptic complex in which one TnpA molecule simultaneously binds two transposon ends. In this complex, TnpA is in an activated state competent for DNA cleavage and strand transfer. Wild-type TnpA can form this complex only on precleaved ends mimicking the initial step of transposition. The data suggest that transposition is controlled at an early stage of transpososome assembly, before DNA cleavage, and that mutations affecting immunity have unlocked TnpA by stabilizing the protein in a monomeric activated synaptic configuration. We propose an asymmetric pathway for coupling active transpososome assembly with proper target recruitment and discuss this model with respect to possible immunity mechanisms.

Hox proteins are conserved homeodomain transcription factors known to be crucial regulators of animal development. As transcription factors, the functions and modes of action (co-factors, target genes) of Hox proteins have been very well studied in a multitude of animal models. However, a handful of reports established that Hox proteins may display molecular activities distinct from gene transcription regulation. Here, we reveal that Hoxa2 interacts with 20S proteasome subunits and RCHY1 (also known as PIRH2), an E3 ubiquitin ligase that targets p53 for degradation. We further show that Hoxa2 promotes proteasome-dependent degradation of RCHY1 in an ubiquitin-independent manner. Correlatively, Hoxa2 alters the RCHY1-mediated ubiquitination of p53 and promotes p53 stabilization. Together, our data establish that Hoxa2 can regulate the proteasomal degradation of RCHY1 and stabilization of p53.

Across three studies, we examined whether and to what extent experiencing abusive supervision leads employees to feel dehumanized by their organization and explored the consequences of this relationship. First, an experimental study manipulating abusive supervision shows that abusive supervision leads to organizational dehumanization perceptions, which in turn have negative consequences (i.e., decreased employees’ job satisfaction, affective commitment, and increased turnover intentions). Based on a cross-lagged panel design, Study 2 confirmed the directionality of the relationship between abusive supervision and organizational dehumanization, by showing the antecedence of abusive supervision on organizational dehumanization. Finally, the results of Study 3 indicated that the indirect effects of organizational dehumanization in the relationships between abusive supervision on the one hand and job satisfaction, affective commitment, and turnover intentions on the other hand are moderated by perceived coworker support.

Post-traumatic epilepsy (PTE) is a major long-term complication of traumatic brain injury (TBI), but early risk prediction remains imprecise. Radiomics enables quantitative analysis of subtle abnormalities on non-contrast head CT (NCCT) that are not readily visible on routine imaging and may improve early risk stratification. This pilot study assessed the performance of radiomic features from acute NCCT, alone or combined with clinical variables, to predict late post-traumatic seizures (PTS) within six months of injury, an early marker of PTE. Eighty-two patients with TBI were included, and two machine-learning approaches were employed: a radiomics-only model and a clinically augmented model incorporating demographics, admission Glasgow Coma Scale (GCS), and prophylactic antiseizure medication use. Radiomics-only models showed moderate discrimination in nested cross-validation (logistic regression AUC = 0.719). Frequently selected features reflected frontal and temporal lobe asymmetry and regional heterogeneity. Adding clinical variables significantly improved performance across all models. The best model, a clinically augmented logistic regression, achieved an AUC of 0.842 with improved accuracy, precision, recall, and F1 score. Admission GCS and antiseizure prophylaxis were the most influential clinical predictors. The findings of this pilot study support NCCT-based radiomics combined with clinical data as a promising framework to be further validated for early PTE risk stratification.