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Circular RNAs (circRNAs) produced from back-spliced exons are widely expressed, but individual circRNA functions remain poorly understood owing to the lack of adequate methods for distinguishing circRNAs from cognate messenger RNAs with overlapping exons. Here, we report that CRISPR–RfxCas13d can effectively discriminate circRNAs from mRNAs by using guide RNAs targeting sequences spanning back-splicing junction (BSJ) sites featured in RNA circles. Using a lentiviral library that targets sequences across BSJ sites of highly expressed human circRNAs, we show that a group of circRNAs are important for cell growth mostly in a cell-type-specific manner and that a common oncogenic circRNA, circFAM120A, promotes cell proliferation by preventing the mRNA for family with sequence similarity 120A (FAM120A) from binding the translation inhibitor IGF2BP2. Further application of RfxCas13d–BSJ-gRNA screening has uncovered circMan1a2, which has regulatory potential in mouse embryo preimplantation development. Together, these results establish CRISPR–RfxCas13d as a useful tool for the discovery and functional study of circRNAs at both individual and large-scale levels.This paper describes a CRISPR–Cas13 system to effectively target circRNAs and screen their functions in vitro and in vivo, which enables the study of relevant circRNA phenotypes in human cell proliferation and in mouse embryogenesis.

Due to the long-term service, Chinese ancient timber buildings show varying degrees of wear. Thus, structural health monitoring (SHM) for these cultural and historical treasures is desperately needed to evaluate the service status. Although there are some FBG sensing-based SHM systems, they are not suitable for Chinese ancient timber buildings due to the differences in architectural types, structural loads, materials, and environment. Besides, a technical gap in Fiber Bragg grating (FBG) sensing-based column inclination monitoring exists. To overcome these weaknesses, this paper develops an FBG sensing-based structural health monitoring system for Chinese ancient Chuan-dou-type timber buildings that aims at monitoring structural deformation, i.e., beam deflection and column inclination, temperature, humidity, and fire around the building. An in-situ test and simulation analyses were conducted to verify the effectiveness of the developed SHM system. To validate the long-term-operation of the developed SHM system, monitoring data within 15 months were analyzed. The results show good agreement between the developed SHM system in this paper and other methods. In addition, the SHM system operated well in the first year after its deployment. This implies that the developed SHM system is applicable and effective in the health state monitoring of Chinese ancient Chuan-dou-type timber buildings, laying a foundation for damage prognosis of such types of timber buildings.

Objective To compare arthroscopy-assisted closed reduction with percutaneous K-wire fixation (AS) versus open reduction with percutaneous K-wire fixation (OR) for juvenile Tillaux fractures Methods We retrospectively reviewed 22 adolescents with displaced Tillaux fractures treated at our institution between 2017 and 2021 (AS, n =8; OR, n =14). Operative time, time to radiographic union, time to full weight-bearing, and American Orthopaedic Foot & Ankle Society (AOFAS) ankle–hindfoot scores at 3 and 6 months were compared. Results Baseline demographics and preoperative displacement were comparable between groups. Operative time was longer in the AS group. The AS group had shorter time to radiographic union and earlier progression to full weight-bearing, and higher AOFAS scores at 3 months; by 6 months, AOFAS scores were similarly excellent in both groups. No major complications were observed in either group. Conclusion In this small retrospective cohort, arthroscopy-assisted reduction was associated with earlier radiographic union and earlier weight-bearing compared with open reduction using the same fixation method, with similarly excellent mid-term functional outcomes. Larger prospective studies with pediatric-validated outcome measures and longer follow-up are warranted.

This study systematically investigates the influence of capacitor energy storage parameters on the energy utilization efficiency of the underwater electrochemical explosion process. By integrating spherical and cylindrical shock wave propagation models, the pulse shock wave energy under different capacitor energy storage levels was theoretically calculated and experimentally validated. The results indicate that the applicability of the shock wave propagation model depends on the distance and aquatic environment: the spherical model is more suitable for short-distance, deep-water conditions, whereas the cylindrical model performs better for long-distance or shallow-water conditions. Within the energy storage range of up to 100 J, increasing the capacitance significantly enhances both the pulse energy output and energy utilization efficiency. Specifically, as the stored energy increased from 13 J to 100 J, the shock wave energy rose from 0.051 J to 2.45 J, and the energy utilization rate improved from 0.39% to 2.45%. Nevertheless, the overall energy utilization efficiency remains below 10%. This study confirms that rationally configuring capacitor parameters can effectively regulate the discharge process, providing important experimental and theoretical support for optimizing energy utilization efficiency.

Magnetic reconnection has been intensively studied in fully ionized plasmas. However, plasmas are often partially ionized in astrophysical environments. The interactions between the neutral particles and ionized plasmas might strongly affect the reconnection mechanisms. We review magnetic reconnection in partially ionized plasmas in different environments from theoretical, numerical, observational and experimental points of view. We focus on mechanisms which make magnetic reconnection fast enough to compare with observations, especially on the reconnection events in the low solar atmosphere. The heating mechanisms and the related observational evidence of the reconnection process in the partially ionized low solar atmosphere are also discussed. We describe magnetic reconnection in weakly ionized astrophysical environments, including the interstellar medium and protostellar discs. We present recent achievements about fast reconnection in laboratory experiments for partially ionized plasmas.

In response to the growing complexity of e-commerce warehouse management driven by the expansion of live-streaming and cross-border businesses, this study tackles the critical challenge of product demand forecasting. We propose an intelligent forecasting approach based on a multi-model fusion framework, constructing a Stacking ensemble that integrates XGBoost, LightGBM, and CatBoost as base learners. Hyperparameter optimization is systematically conducted using grid search combined with cross-validation. To account for periodic trends, seasonal fluctuations are modeled as explicit temporal features, and a Seasonal Autoregressive Integrated Moving Average (SARIMA) model is incorporated to perform joint forecasting by capturing residual time-dependent patterns. Experimental results show that the proposed fusion model consistently outperforms all individual base learners across multiple metrics, including R2, RMSE, MAE, MAPE, Precision, Recall, and Accuracy. Furthermore, the mean cosine similarity of the forecast sequences reaches 0.986, underscoring both the stability of seasonal representations and the model’s robustness in capturing demand variations. This method effectively improves the accuracy of e-commerce product demand forecasts, offering reliable data support for inventory management and allocation strategies.

Circular intronic RNAs (ciRNAs) escaping from DBR1 debranching of intron lariats are co-transcriptionally produced from pre-mRNA splicing, but their turnover and mechanism of action have remained elusive. We report that RNase H1 degrades a subgroup of ciRNAs in human cells. Many ciRNAs contain high GC% and tend to form DNA:RNA hybrids (R-loops) for RNase H1 cleavage, a process that appears to promote Pol II transcriptional elongation at ciRNA-producing loci. One ciRNA, ciankrd52 , shows a stronger ability of R-loop formation than that of its cognate pre-mRNA by maintaining a locally open RNA structure in vitro . This allows the release of pre-mRNA from R-loops by ci-ankrd52 replacement and subsequent ciRNA removal via RNase H1 for efficient transcriptional elongation. We propose that such an R-loop dependent ciRNA degradation likely represents a mechanism that on one hand limits ciRNA accumulation by recruiting RNase H1 and on the other hand resolves R-loops for transcriptional elongation at some GC-rich ciRNA-producing loci.

The prevalence of childhood obesity has been increasing worldwide, garnering increasing public attention due to various complications and long-term effects. Many researchers have indicated that obese children experienced higher fracture risk compared with nonobese children. However, the findings from different researchers reported a controversial result and few of them paid attention to the differences in BMI Z-score and obesity between different fracture sites, which would be quite useful since these can guide obese children in protecting specific regions. This study comprised 17,942 hospitalized children diagnosed with fractures and 3219 healthy children who came for physical examination and had not been diagnosed with any illness. Data on age, gender, fracture seasons, fracture sites, height, weight, total cholesterol, triacylglycerol, and high-density lipoprotein were collected. One-way ANOVA, t-test, Chi-square test, propensity score matching, and logistic regression models were used in statistical analysis. Children with lower limb fractures exhibited the highest BMI Z-score (0.58 ± 1.74), followed by those with upper limb fractures (0.50 ± 1.52), axial fractures (0.31 ± 1.56), and head fractures (-0.02 ± 1.52). In terms of obesity, children with lower limb fractures exhibited the highest obesity rate (20.20%), whereas those with upper limb fractures (15.61%) and axial fractures (14.96%) displayed comparable obesity rates. Children with head fractures had the lowest obesity rate (8.42%). Moreover, BMI Z-score (2.43 ± 1.22vs0.15 ± 1.43, P < 0.001), obesity (62.97%vs8.61%, P < 0.001) and dyslipidemia (31.22%vs24.67%, P < 0.001) were statistically significant difference between the fracture and healthy groups. The logistic regression models showed that BMI Z-score was associated with an increased risk of fracture (P < 0.001, OR = 4.89, 95%CI: 4.53–5.27). This study suggests that children with lower limb fractures exhibited the highest BMI Z-score and obesity rate, while those with head fractures had the lowest BMI Z-score and obesity rate. When compared with the healthy group, fracture children had higher BMI Z-score, obesity, and dyslipidemia rates. In addition, BMI Z-score was associated with an increased risk of fractures.

The Fiber Bragg Grating (FBG) sensing technique is suitable for a wide variety of measurements, including temperature, pressure, acceleration, liquid level, etc., and has been applied to many bridges and buildings in the past two decades. The fact that the FBG technique can only monitor and measure strain data for most cases when it is used for deformation measurements impedes application of the FBG sensing technique in civil infrastructures. This paper proposes FBG sensing-based deformation monitoring methods that are applicable to monitoring beam deflection, column inclination angle and mortise-tenon joint dislocation for Chinese traditional timber structures. On the basis of improved conjugated beam theory and geometrical trigonometric function relationship, the relationships between the FBG sensing strain values and the deflection of beam, inclination angle of column, as well as the amount of dislocation of mortise-tenon joint are deducted for Chinese traditional buildings. A series of experiments were conducted to verify the applicability and effectiveness of the proposed deformation monitoring methods. The results show that a good agreement is obtained between the values given by the methods proposed in this paper and other methods. This implies that the proposed deformation monitoring methods are applicable and effective in the health monitoring of Chinese traditional timber structures.

Understanding the mechanisms that promote cooperative behaviors of bacteria in their hosts is of great significance to clinical therapies. Environmental stress is generally believed to increase competition and reduce cooperation in bacteria. Here, we show that bacterial cooperation can in fact be maintained because of environmental stress. We show that Pseudomonas aeruginosa regulates the secretion of iron-scavenging siderophores in the presence of different environmental stresses, reserving this public good for private use in protection against reactive oxygen species when under stress. We term this strategy “conditional privatization”. Using a combination of experimental evolution and theoretical modeling, we demonstrate that in the presence of environmental stress the conditional privatization strategy is resistant to invasion by non-producing cheaters. These findings show how the regulation of public goods secretion under stress affects the evolutionary stability of cooperation in a pathogenic population, which may assist in the rational development of novel therapies. Pyoverdine is secreted by the opportunistic pathogen Pseudomonas aeruginosa to scavenge iron from its hosts. Here, the authors show that under stress conditions P. aeruginosa uses a ‘conditional privatization’ strategy, reserving pyoverdine intracellularly to protect against oxidative damage.