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This study aimed to explore the RNA viruses affecting Passiflora species in Brazil. Our results enhance the understanding of the viruses that infect Passiflora plants by identifying and characterizing three previously unrecognized viruses: Passiflora cytorhabdovirus (PFCV), Passiflora nucleorhabdovirus 1 (PaNV1), and Passiflora nucleorhabdovirus 2 (PaNV2). These rhabdoviruses were identified through high-throughput sequencing and validated by reverse transcription-polymerase chain reaction (RT-PCR) in various Passiflora species. PFCV has a genome organization 3′-N-P-P3-P4-M-G-P7-L-5′ and was classified as a novel member of the Gammacytorhabdovirus genus. A particularly noteworthy feature of PFCV is its glycoprotein, as the genomes of other gammarhabdoviruses do not contain this gene. PFCV has a high incidence across multiple locations and was identified in plants from Northeastern, Central, and Southeastern Brazil. PaNV1 with genome structure 3′-N-P-P3-M-G-L-5′ and PaNV2 with genome organization 3′-N-X-P-Y-M-G-L-5′ are new members of the Alphanucleorhabdovirus genus and have a more restricted occurrence. Importantly, all three viruses were found in mixed infections alongside at least one other virus. In situ observations confirmed mixed infections, with PaNV2 particles co-located in tissues with a potyvirus and a carlavirus. Phylogenetic and glycoprotein sequence similarity network analysis provided insights into their evolutionary placement and potential vector associations. These findings expand the known diversity of rhabdoviruses in Passiflora and contribute to the understanding of their evolution and epidemiology.

ABSTRACT Objectives: to analyze undergraduate nursing course coordinators’ perception about nursing training in palliative care. Methods: a descriptive study, with a qualitative approach and thematic content analysis, carried out with coordinators of nursing courses in Higher Education Institutions in Rio Grande do Norte. Results: three thematic categories emerged: Nursing training in palliative care; Potentialities for teaching palliative care; and Challenges of teaching in palliative care. The coordinators described as potentialities: transversality, theoretical and practical approach, optional subject, university extensions, interdisciplinarity and transdisciplinary approach, and as challenges: biomedical model in health education and insufficient professor training. Final Considerations: palliative care teaching in the researched institutions in the nursing education process is approached in an incipient and fragmented way, and almost always without having a specific curricular component on the subject, being present as one of its contents. RESUMEN Objetivos: analizar la percepción de los coordinadores de cursos de graduación en enfermería sobre la formación de enfermería en cuidados paliativos. Métodos: estudio descriptivo, con abordaje cualitativo y análisis de contenido temático, realizado con coordinadores de cursos de enfermería en Instituciones de Educación Superior de Rio Grande do Norte. Resultados: surgieron tres categorías temáticas: Formación de enfermeros en cuidados paliativos; Potencialidades para la enseñanza de cuidados paliativos; y Desafíos de la enseñanza en cuidados paliativos. Los coordinadores describieron como potencialidades: transversalidad, enfoque teórico y práctico, materia optativa, extensiones universitarias, interdisciplinariedad y abordaje transdisciplinar, y como desafíos: modelo biomédico en educación para la salud e insuficiente formación docente. Consideraciones Finales: la enseñanza de los cuidados paliativos en las instituciones investigadas en el proceso de formación de enfermería es abordada de forma incipiente y fragmentada, y casi siempre sin tener un componente curricular específico sobre el tema, estando presente como uno de sus contenidos. RESUMO Objetivos: analisar a percepção dos coordenadores de curso de graduação em enfermagem sobre a formação do enfermeiro em cuidados paliativos. Métodos: estudo descritivo, de abordagem qualitativa e análise de conteúdo temática, realizado com coordenadores dos cursos de enfermagem em Instituições de Ensino Superior do Rio Grande do Norte. Resultados: emergiram três categorias temáticas: Formação do enfermeiro em cuidados paliativos; Potencialidades para o ensino dos cuidados paliativos; e Desafios do ensino em cuidados paliativos. Os coordenadores descreveram como potencialidades: transversalidade, abordagem teórica e prática, disciplina optativa, extensões universitárias, interdisciplinaridade e abordagem transdisciplinar, e como desafios: modelo biomédico na formação em saúde e formação docente insuficiente. Considerações Finais: o ensino dos cuidados paliativos nas instituições pesquisadas no processo de formação do enfermeiro é abordado de forma incipiente e fragmentado, e quase sempre sem ter um componente curricular específico sobre o tema, estando presente como um de seus conteúdos.

Mitoviruses are cryptic capsidless viruses belonging to the family Mitoviridae that replicate and are maintained in the mitochondria of fungi. Complete mitovirus-like sequences were recently assembled from plant transcriptome data and plant leaf tissue samples. Passion fruit (Passiflora spp.) is an economically important crop for numerous tropical and subtropical countries worldwide, and many virus-induced diseases impact its production. From a large-scale genomic study targeting viruses infecting Passiflora spp. in Brazil, we detected a de novo-assembled contig with similarity to other plant-associated mitoviruses. The contig is ∼2.6 kb long, with a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRP). This contig has been named \"passion fruit mitovirus-like 1\" (PfMv1). An alignment of the predicted amino acid sequence of the RdRP of PfMv1 and those of other plant-associated mitoviruses revealed the presence of the six conserved motifs of mitovirus RdRPs. PfMv1 has 79% coverage and 50.14% identity to Humulus lupulus mitovirus 1. Phylogenetic analysis showed that PfMV1 clustered with other plant-associated mitoviruses in the genus Duamitovirus. Using RT-PCR, we detected a PfMv1-derived fragment, but no corresponding DNA was identified, thus excluding the possibility that this is an endogenized viral-like sequence. This is the first evidence of a replicating mitovirus associated with Passiflora edulis, and it should be classified as a member of a new species, for which we propose the name \"Duamitovirus passiflorae\".

PurposeTo evaluate the accuracy of a positive self-reported glaucoma family history.Material and methodsCross-sectional study. Each subject was asked if they had a first-degree relative diagnosed with glaucoma. If their answer was affirmative, the relative was invited to attend on ophthalmic evaluation and underwent complementary exams to confirm or exclude the glaucoma diagnosis. Only one relative was included per subject.ResultsWe included 204 subjects in the study (102 subjects and their respective relatives). The accuracy of family history of glaucoma was 76.96% of the cases. In the univariable analysis, subjects with college degree had 2.34 [(P = 0.010; 95% confidence interval (CI) 1.18–4.63)], with higher family income 3.72 (P = 0.003; 95% CI 1.57–8.85) and those with health insurance 3.42 (P = 0.001; 95% CI 1.67–6.98) more chances to have a true positive family history for glaucoma. In the multivariable logistic regression analysis, none of the variables presented significant association.ConclusionAround 24% of patients may not provide reliable information about family history for glaucoma. When asking about a glaucoma family history, clinicians should consider the real accuracy of this self-reported data.

Introduction: Chronic non-communicable diseases are considered the main causes of death and disability, both nationally and worldwide. They have multifactorial causes, and the main ones are cardiovascular diseases, cerebrovascular accidents, chronic respiratory diseases, diabetes mellitus and neoplasms.Objective: To describe the self-care of users with non-communicable chronic diseases in Primary Health Care, in the light of Orem's Self-Care Theory.Method: Cross-sectional, descriptive and exploratory study carried out in an area covered by a Family Health Unit in the city of Natal, Rio Grande do Norte, Brazil. The non-probabilistic and intentional sample had 80 users with chronic health conditions. Data were collected between January 2018 and December 2019, through a structured interview and through the use of a validated form for the analysis of self-care in adults. The results, presented in absolute and relative frequencies, have a confidence interval of 95%.Results: Among the sociodemographic variables, it was identified that 70% of users had elementary education and that 85% had cardiovascular diseases. As for self-care, all users were independent to perform body hygiene and to control bladder and bowel eliminations. 98.8% were independent to dress themselves; 96.3%, for walking; 87.5%, to chew and swallow; and 83.8%, to prepare food.Conclusion: Independence for activities of daily living and self-care were characteristics present among the participants. Such independence is considered important in the Self-Care Theory. Introducción: Las enfermedades crónicas no transmisibles son consideradas las principales causas de muerte y discapacidad, tanto a nivel nacional como mundial. Tienen causas multifactoriales y las principales son las enfermedades cardiovasculares, los accidentes cerebrovasculares, las enfermedades respiratorias crónicas, la diabetes mellitus y las neoplasias.Objetivo: Describir el autocuidado de usuarios con enfermedades crónicas no transmisibles en la Atención Primaria de Salud a la luz de la Teoría del Autocuidado de Orem.Método: Estudio transversal, descriptivo y exploratorio, realizado en un área anexa de una Unidad de Salud de la Familia, en la ciudad de Natal, Rio Grande do Norte, Brasil. La muestra no probabilística e intencional estuvo conformada por 80 usuarios con condiciones crónicas de salud. Los datos fueron recolectados entre enero de 2018 y diciembre de 2019, a través de una entrevista estructurada y el uso de un formulario validado para el análisis del autocuidado en adultos. Los resultados, presentados en frecuencias absolutas y relativas, e intervalo de confianza del 95%.Resultados: Entre las variables sociodemográficas, se identificó que 70% de los usuarios tenían instrucción básica y padecían enfermedades cardiovasculares 85%; en cuanto al autocuidado, todos los usuarios eran independientes para realizar la higiene corporal y controlar las deposiciones vesicales e intestinales; El 98,8% eran independientes para vestirse, el 96,3% para caminar, el 87,5% para masticar y tragar y el 83,8% para preparar la comida.Conclusión: La independencia para las actividades de la vida diaria y el autocuidado fueron características presentes entre los participantes, consideradas importantes en la Teoría del Autocuidado. Introdução: As doenças crônicas não transmissíveis são consideradas as principais causas de morte e de incapacidades, tanto a nível nacional quanto mundial. Elas possuem causas multifatoriais, e as principais delas são as doenças cardiovasculares, os acidentes cerebrovasculares, as doenças respiratórias crônicas, diabetes mellitus e neoplasias.Objetivo: Descrever o autocuidado de usuários com doenças crônicas não transmissíveis na Atenção Primária à Saúde, à luz da Teoria do Autocuidado de Orem. Método: Estudo transversal, descritivo e exploratório, alizado em área adscrita de uma Unidade de Saúde da Família, no município de Natal, Rio Grande do Norte, Brasil. A amostra não probabilística e intencional contou com 80 usuários em condições crônicas de saúde. Os dados foram coletados entre janeiro de 2018 e dezembro de 2019, por meio de entrevista estruturada e por meio de uso de formulário validado para a análise do autocuidado em adultos. Os resultados, apresentados em frequências absolutas e relativas, possuem intervalo de confiança de 95%. Resultados: Dentre as variáveis sociodemográficas, identificou-se que 70% dos usuários têm ensino fundamental e que 85% apresentam doenças cardiovasculares. Quanto ao autocuidado, todos os usuários eram independentes para realizar higiene corporal e para controlar as eliminações vesicais e intestinais. 98,8% eram independentes para vestir-se; 96,3%, para deambular; 87,5%, para mastigar e deglutir; e 83,8%, para preparar os alimentos. Conclusão: A independência para as atividades de vida diária e de autocuidado foram características presentes entre os participantes. Tal independência é considerada importante na Teoria do Autocuidado.

Natural infections of LCV have been identified in lettuce, beets, papaya, and cilantro in the USA (Alabi et al. 2017, 2019; Duffus et al. 1996; Wisler et al. 1997), in papaya in Mexico (Alcalá-Briseño et al. 2020), beans in Spain (Ruiz et al. 2013), cannabis in Israel (Hadad et al. 2019), and tomato, tobacco, and periwinkle in China (Tian et al. 2017; Zhang et al. 2017; Zhao et al. 2018). The Passiflora germplasm bank “Flor da Paixão” (BAG-FP) is maintained at Embrapa Cerrados, in Brasília, DF, Brazil, and is intended for the conservation, characterization, and research on Passiflora genetic resources, including passionflower breeding programs. The micro-spin column was assembled by piercing a small hole in the bottom of a capless 0.6 ml microtube, adding 30 mg of glass wool (medium fiber, CAS 65997173-Dinâmica) and 120 mg of cellulose (cellulose fibers, medium, C6288- SIGMA) at the bottom of the tube. Analysis of genome-partial pairwise identities revealed that the LCV-RNA1 and LCV-RNA2 from P. auriculata (MN564796, MN564795) and P. alata (MN564797-MN564798) share > 96% nucleotide identity for both RNAs with LCV isolates of lettuce (FJ380118, FJ380119) and papaya (KY271955, KY271956) from the USA, and the isolate of periwinkle (MN216391, MN216392) from Brazil.

Introdução: As doenças crônicas não transmissíveis são consideradas as principais causas de morte e de incapacidades, tanto a nível nacional quanto mundial. Elas possuem causas multifatoriais, e as principais delas são as doenças cardiovasculares, os acidentes cerebrovasculares, as doenças respiratórias crônicas, diabetes mellitus e neoplasias. Objetivo: Descrever o autocuidado de usuários com doenças crônicas não transmissíveis na Atenção Primária à Saúde, à luz da Teoria do Autocuidado de Orem. Método: Estudo transversal, descritivo e exploratório, realizado em área adscrita de uma Unidade de Saúde da Família, no município de Natal, Rio Grande do Norte, Brasil. A amostra não probabilística e intencional contou com 80 usuários em condições crônicas de saúde. Os dados foram coletados entre janeiro de 2018 e dezembro de 2019, por meio de entrevista estruturada e por meio de uso de formulário validado para a análise do autocuidado em adultos. Os resultados, apresentados em frequências absolutas e relativas, possuem intervalo de confiança de 95%. Resultados: Dentre as variáveis sociodemográficas, identificou-se que 70% dos usuários têm ensino fundamental e que 85% apresentam doenças cardiovasculares. Quanto ao autocuidado, todos os usuários eram independentes para realizar higiene corporal e para controlar as eliminações vesicais e intestinais. 98,8% eram independentes para vestir-se; 96,3%, para deambular; 87,5%, para mastigar e deglutir; e 83,8%, para preparar os alimentos. Conclusão: A independência para as atividades de vida diária e de autocuidado foram características presentes entre os participantes. Tal independência é considerada importante na Teoria do Autocuidado.

ABSTRACT Objectives: to understand the structure of the social representations of health professionals from HIV/AIDS Specialized Care Services about HIV-positive partners. Methods: this is a qualitative study, based on the structural aspect of Social Representations, developed in specialized services of the metropolitan area of a state in the Northeast Region. Fifty-one professionals were interviewed using the technique of free association of words, processed by the software IRaMuTeQ, by means of prototypical and similarity analysis. Results: the central nucleus was constituted by the terms “partnership”, “love” and “fear”, showing appreciation of meanings inherent to their beliefs, values and experiences that bring possibilities of reflections for health practices. Final Considerations: the findings reinforce the impacts generated in the different segments of the lives of people living with HIV and in their emotional bonds. This highlights the need for the implementation of care strategies contemplating the biopsychosocial care model rather than the biological model. RESUMEN Objetivos: aprender estructura de representaciones sociales de personal de salud de Servicios de Asistencia Especializada VIH/SIDA sobre parejas que viven en serodiferencia cuanto al VIH. Métodos: estudio cualitativo, fundamentado en la vertiente estructural de Representaciones Sociales, desarrollado en servicios especializados de la región metropolitana de un estado del Nordeste. Entrevistados 51 profesionales con aplicación de técnica de asociación libre de palabras, procesadas por software IRaMuTeQ, mediante análisis prototípico y de similitud. Resultados: el núcleo central constituido por los términos “compañerismo”, “amor” y “miedo”, evidenciando valorización de sentidos inherentes a sus creencias, valores y experiencias que traen posibilidades de reflexiones para las prácticas de salud. Consideraciones Finales: hallados refuerzan los impactos generados en los diferentes segmentos de la vida de las personas que viven con VIH y en sus vínculos afectivos. Eso señala la necesidad de implementación de estrategias asistenciales contemplando el modelo de cuidado biopsicosocial en detrimento del modelo biológico. RESUMO Objetivos: apreender a estrutura das representações sociais de profissionais de saúde dos Serviços de Assistência Especializada HIV/aids sobre os parceiros que vivem em sorodiferença quanto ao HIV. Métodos: estudo qualitativo, fundamentado na vertente estrutural das Representações Sociais, desenvolvido em serviços especializados da região metropolitana de um estado da Região Nordeste. Entrevistaram-se 51 profissionais com aplicação da técnica de associação livre de palavras, processadas pelo software IRaMuTeQ, mediante análise prototípica e de similitude. Resultados: o núcleo central foi constituído pelos termos “parceria”, “amor” e “medo”, evidenciando valorização de sentidos inerentes às suas crenças, valores e experiências que trazem possibilidades de reflexões para as práticas de saúde. Considerações Finais: os achados reforçam os impactos gerados nos diferentes segmentos da vida das pessoas que vivem com o HIV e em seus vínculos afetivos. Isso ressalta a necessidade da implementação de estratégias assistenciais contemplando o modelo de cuidado biopsicossocial em detrimento do modelo biológico.

Yellow passion fruit (Passiflora edulis Sims) yield and longevity have been drastically reduced by bacterial spot (Xanthomonas axonopodis pv. passiflorae- Xap) and passion fruit woodiness disease (PWD) (Cowpea aphid-borne mosaic virus – CABMV). This study was aimed at evaluating the reaction of 11 genotypes of yellow passion fruit, based on the reaction of their progenies, to both mechanically inoculated Xap and CABMV, under greenhouse conditions. There was a progressive increase in bacterial spot and PWD severity with time. BRS Gigante Amarelo, MAR20#12, and MAR20#34 were selected as the progenies with lowest bacterial spot severity and disease progress over time. MAR20#2005, EC-L-7, UnB2015-1, and EC-3-0 presented the lowest PWD severity and disease progress over time. These progenies, along with individual plants from other progenies in which disease severity was significantly low until the last evaluation, will be cloned and tested again for Xap and CABMV, including other isolates. Get the article here. (PDF)

Problem: The absence of facilitating stimuli in the environment in which the adolescent coexists is usually related to a delay in his development. Objective: To analyze the association of factors related to the \"Development Delay\" diagnostic proposal among adolescents in the school environment. Method: Transversal study performed with 385 adolescents in a city in Northeastern Brazil. The study was approved by the Ethics in Research Committee of the responsible institution, under opinion no 1.662.528 and the Certificate of Presentation for Ethical Consideration no 57945016.4.0000.5537. Outcomes: The prevalence of the diagnostic proposition for delay in development was of 18.26%. Related factors that showed significant statistical association were social marginalization, chronic pain, refusal to accept body changes taking place during puberty and psychological trauma. Discussion: One can perceive the relation between the studied variables and human development when considering the literature available. Conclusion: The knowledge of the associated factors most present in this population facilitates the understanding of the diagnostic proposal by the nurse. Besides, it contributes to the identification of the different aspects capable of causing harm to the adolescent and, consequently sequels in the adult life. Describers: Nursing; Nursing diagnostic; Adolescent.