Explore the vast range of titles available.

Background Soybean is an extensively utilized oilseed crop, and improved cultivars and cultivation efficiency of soybean have contributed to the increased use of soybean in edible oil applications. The food industry necessitates the development of soybean oil with an optimized balance of polyunsaturated and saturated fatty acids to meet both nutritional requirements and industrial applications. Results This study aimed to elucidate the protein structure and functional characterization of a novel allele of KASII-A derived from an EMS-induced mutant line and assess its potential as a genetic resource for developing soybean cultivars with elevated saturated fatty acid composition. Sequence variation in the KASII-A gene was evaluated for PE1544 (~ 16.1% palmitic acid composition), an EMS-induced mutant with high-palmitic acid. A single-nucleotide polymorphism was identified in the KASII-A gene of PE1544, resulting in an amino acid substitution from Gly309 to Asp309. Comparative analysis of three-dimensional protein structures revealed that Gly309 plays a critical role in stabilizing the catalytic residue in the KASII-A active site. Co-segregation analysis revealed that the novel allele was recessive to KASII-A and was associated with high-palmitic acid composition. Furthermore, we analyzed the F 2 population derived from the cross between the high-stearic acid line with homozygous recessive sacpd-c allele and PE1544. The F 2 progeny with both mutations exhibited a lower stearic acid composition compared to the single sacpd-c mutant. Notably, the F 2 progeny with both mutations exhibited a similar ratio of polyunsaturated to saturated fatty acids (P/S index) compared to the single sacpd-c mutant. These findings suggest that KASII-A regulates the palmitic acid and stearic acid composition regardless of the total composition of saturated fatty acids in the single sacpd-c mutant. Comprehensively, the regulation of KASII-A in the single sacpd-c mutant is effective for the development of soybean oil with an ideal P/S index by regulating the content of palmitic and stearic acid while maintaining high-saturated fatty acids. Conclusion These results suggest that the conversion of palmitic acid to stearic acid is impaired due to the loss-of-function of KASII-A, indicating that the novel allele of KASII-A plays a crucial role in this biochemical conversion in soybean.

Soybean seed oils contain approximately 23% oleic acid, and elevated amounts of oleic acid help prevent cardiovascular diseases and improve the quality of the oil. Chemically, it helps maintain the oxidative stability of oil; hence, soybean breeders primarily seek to increase its concentration for improved oil quality. As soybean seeds with mutant alleles of FAD2-1A and FAD2-1B genes have been reported to produce approximately 80% of oleic acid, a mutant population was developed from an ethyl methanesulfonate (EMS)-induced soybean cultivar (Pungsannamul). From this, a new mutant allele of FAD2-1A was identified using mutant lines with elevated oleic acid levels and the pooled-DNA sequencing method. This study identified PE529 as the allele with >40% oleic acid carrying the novel allele of the FAD2-1A gene. The single nucleotide polymorphism (SNP) in PE529 also induced the conversion from tryptophan to a premature stop codon at position 293 in the amino acid sequence (W293STOP). The inheritance analysis showed that the elevated oleic acids in PE529 were attributed to the fad2-1a W293STOP allele. In this study, seeds capable of producing approximately 80.0% oleic acid were identified from F2 populations where fad2-1a W293STOP and fad2-1b alleles were segregated. Hence, soybeans with novel alleles are useful genetic resources to improve soybean oil quality in breeding programs.

Soybean seed oils contain approximately 23% oleic acid, and elevated amounts of oleic acid help prevent cardiovascular diseases and improve the quality of the oil. Chemically, it helps maintain the oxidative stability of oil; hence, soybean breeders primarily seek to increase its concentration for improved oil quality. As soybean seeds with mutant alleles of FAD2-1A and FAD2-1B genes have been reported to produce approximately 80% of oleic acid, a mutant population was developed from an ethyl methanesulfonate (EMS)-induced soybean cultivar (Pungsannamul). From this, a new mutant allele of FAD2-1A was identified using mutant lines with elevated oleic acid levels and the pooled-DNA sequencing method. This study identified PE529 as the allele with >40% oleic acid carrying the novel allele of the FAD2-1A gene. The single nucleotide polymorphism (SNP) in PE529 also induced the conversion from tryptophan to a premature stop codon at position 293 in the amino acid sequence (W293STOP). The inheritance analysis showed that the elevated oleic acids in PE529 were attributed to the fad2-1a W293STOP allele. In this study, seeds capable of producing approximately 80.0% oleic acid were identified from F[sub.2] populations where fad2-1a W293STOP and fad2-1b alleles were segregated. Hence, soybeans with novel alleles are useful genetic resources to improve soybean oil quality in breeding programs.