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Well-being is a continuous process requiring decisions and actions to maintain or build health. This study examines the indirect effect of health locus of control on life satisfaction via healthy behaviors based on living systems theory. Participants were 730 students of various majors related to medicine and health, aged between 19 and 30 ( M  = 22.29, SD  = 1.64), including 79.04% women. Self-report measures were used to assess life satisfaction, multidimensional health locus of control, and health behavior. Women scored higher than men on the total health behavior scale, especially in a healthy diet and preventive behavior. Men scored higher than women on internal health locus of control (HLC), while women scored higher than men on the powerful others HLC in making decisions about their health. The study confirmed the mediating effect of healthy behavior on the relationship between the internal HLC and life satisfaction. The present mechanism highlights the crucial role of internal motivation in increasing subjective well-being by maintaining health behaviors among young adults. The role of intervention programs focused on improving internal HLC and health behaviors is discussed.

The aim of our study was to explore the relation between physical activity, sedentary behavior, and the subjective and objective indicators of quality of life as well as life satisfaction among university students, whose education is related to different dimensions on health. Participants (N = 595) were invited to fill in a set of suitable questionnaires. The path analysis and linear regression were used to establish a relationship between the examined constructs. Only some types of physical activity have shown a positive relation with the quality of life; the study also revealed some age and gender regularities. Physical activity in the household was most positively correlated to the quality of life. The amount of leisure and transport physical activity decreased with age, and there were also gender differences regarding the intensity and type of physical activity. Sedentary behavior during the week related positively with the subjective quality of life and its intimacy dimension, but sedentary behavior at the weekends was negatively related to objective and subjective quality of life as well as dimensions including intimacy, safety, and communicative aspect of the quality of life. Neither physical activity nor sedentary behavior demonstrated a significant relation with the level of life satisfaction. The type of physical activity undertaken and its matching to the needs of the young person affected their objective and subjective quality of life. Those findings may have important implications for institutions responsible for promoting active lifestyle.

In 1972, J. Woodland Hastings and colleagues predicted the existence of a proton selective channel (HV1) that opens in response to depolarizing voltage across the vacuole membrane of bioluminescent dinoflagellates and conducts protons into specialized luminescence compartments (scintillons), thereby causing a pH drop that triggers light emission. HV1 channels were subsequently identified and demonstrated to have important functions in a multitude of eukaryotic cells. Here we report a predicted protein from Lingulodinium polyedrum that displays hallmark properties of bona fide HV1, including time-dependent opening with depolarization, perfect proton selectivity, and characteristic ΔpH dependent gating. Western blotting and fluorescence confocal microscopy of isolated L. polyedrum scintillons immunostained with antibody to LpHV1 confirm LpHV1's predicted organellar location. Proteomics analysis demonstrates that isolated scintillon preparations contain peptides that map to LpHV1. Finally, Zn2+ inhibits both LpHV1 proton current and the acid-induced flash in isolated scintillons. These results implicate LpHV1 as the voltage gated proton channel that triggers bioluminescence in L. polyedrum, confirming Hastings' hypothesis. The same channel likely mediates the action potential that communicates the signal along the tonoplast to the scintillon.

Background The level of health behavior of physical education teachers and people preparing to perform this profession is important from the point of view of the need to play the role of a health promoter – an authority in the promotion of health culture. The aim of the study was to assess the level of teachers’ health behaviors of physical education teachers and students, as well as to search for their determinants. Material and Methods The research covered 127 teachers and 173 physical education students. Standardized questionnaire tools were used to assess the level of health behaviors ( Juczyński Health Behavior Inventory ) and physical activity ( International Physical Activity Questionnaire ). Results Teachers demonstrate a higher level of health behaviors than students. Most respondents achieved an average level of the general health behavior index (HBI) (53.1% of teachers, 52.3% of students), while a high level – 26.8% of teachers, 19.8% of students and a low level – 19.9% of teachers and 27.7% of students. Female gender and teaching profession are significant predictors of HBI. Physical activity, both its current level and the level undertaken in the past, as well as the body mass index (BMI) do not determine HBI. In the regression model, physical activity is significantly associated with male gender, past sports activity and a lower BMI level. Conclusions Research indicates neglect in the general area of health behaviors among men, while among women, physical activity needs to be more widely popularized. The teacher education system should be more focused on the development of self-development competencies in the context of health-promoting behaviors. Med Pr Work Health Saf. 2024;75(4):355–365

The blood fluke Cardicola forsteri (Trematoda: Aporocotylidae) is a pathogen of ranched bluefin tuna in Japan and Australia. Genomics of Cardicola spp. have thus far been limited to molecular phylogenetics of select gene sequences. In this study, sequencing of the C . forsteri genome was performed using Illumina short-read and Oxford Nanopore long-read technologies. The sequences were assembled de novo using a hybrid of short and long reads, which produced a high-quality contig-level assembly (N50 > 430 kb and L50 = 138). The assembly was also relatively complete and unfragmented, comprising 66% and 7.2% complete and fragmented metazoan Benchmarking Universal Single-Copy Orthologs (BUSCOs), respectively. A large portion (> 55%) of the genome was made up of intergenic repetitive elements, primarily long interspersed nuclear elements (LINEs), while protein-coding regions cover > 6%. Gene prediction identified 8,564 hypothetical polypeptides, > 77% of which are homologous to published sequences of other species. The identification of select putative proteins, including cathepsins, calpains, tetraspanins, and glycosyltransferases is discussed. This is the first genome assembly of any aporocotylid, a major step toward understanding of the biology of this family of fish blood flukes and their interactions within hosts.

The study attempted to answer the question of whether the type of practiced sport (individual, team) is a determinant of quality of life in athletes. The study was also intended to identify the best quality of life determinants in professional karate practitioners and team sports players from among such socio-cultural factors as age, gender, marital status, material situation, level of education, and types of health behaviors. The study involved 110 Polish players of team sports and 90 martial arts practitioners. All competitors had high sporting achievements. A Paper-and-Pen Interview questionnaire was used as a standardized survey method. The Inventory of Health Behaviors was used to evaluate respondents' health behaviors. The Comprehensive Quality of Life Scale was used to measure the quality of life in its objective and subjective components. Among the predictors of quality of life, only three proved to be statistically significant: proper dietary habits (β = 0.204), positive mental attitude (β = 0.283) and athletes' gender (β = 0.191). It turns out that men are statistically more likely to achieve a higher quality of life than women. The type of practiced sport did not turn out to be a quality of life determinant. The type of practiced sport is not a significant quality of life predictor, perhaps because high-level sports activities are professional in nature, which makes the professional and sports activities uniform. The results of the study may suggest that the determinants of the quality of life of professional athletes are found in their personality rather than solely in their socio-cultural environment. Finally, studies of determinants of athletes' quality of life can help develop optimal strategies for improving the quality of life in society in general; however, in-depth qualitative research also seems to be necessary to do it.

This study examines the indirect relationship between optimism and life satisfaction via healthy behavior among health sciences students. The cross-sectional study involved 349 health sciences students, including 58% of women, ranging in age between 19 and 30 years ( = 22.15, = 1.83). Self-report questionnaires were used to measure dispositional optimism (the Life Orientation Test-Revised, LOT-R), life satisfaction (Satisfaction with Life Scale, SWLS), and health behaviors (Health Behavior Inventory, HBI), with four subscales: healthy diet (HD), preventive behavior (PB), positive mental attitudes (PMA), and healthy practices (HP). In addition, a single mediation model (with the total HBI as mediator) was compared with a parallel mediation model (with four subscales of the HBI). Gender was controlled as a confounding variable. Women scored higher in the total HBI ( < 0.01), HD ( < 0.001) and PB ( < 0.01) than men, while men scored slightly higher in dispositional optimism ( < 0.01). General health behavior (total HBI) completely mediates the relationship between dispositional optimism and life satisfaction, = 0.32. In the parallel model, only PMA was determined as a mediator of the association between dispositional optimism and satisfaction with life, = 0.36. The single mediation model showed perfect fit (X / = 0.00, RMSEA = 0.00, SRMR = 0.00, CFI = 1.00, IFI = 1.00, NFI = 1.00), better than the parallel mediation model (X / = 2.353, = 0.095, RMSEA = 0.062, SRMR = 0.020, CFI = 0.995, IFI = 995, NFI = 991). The interplay mechanism between personal resources and behavioral health-related habits may explain 32% of life satisfaction variance. The intervention focused on increasing optimistic expectancies and health behaviors should be implemented in universities to increase students' well-being and prevent depression.

Farmed fish are regularly subjected to various stressors due to farming practices, and their effect in the context of a disease outbreak is uncertain. This research evaluated the effects of unpredictable repeated stress in rainbow trout challenged with the ciliate Ichthyophthirius multifiliis, known to cause white spot disease in freshwater fish. Before and after the pathogen exposure, fish were handled with a random rotation of three procedures. At 7 days post-infection (dpi), the parasite burden was evaluated in fish and in the tank’s water, and the local and systemic immune responses were investigated in the gill and spleen, respectively. The fish mortality was recorded until 12 dpi, when all the fish from the infected groups died. There was no statistical difference in parasite burden (fish and tank’s water) and infection severity between the two infected fish groups. The immune gene expression analysis suggested a differential immune response between the gill and the spleen. In gills, a T helper cell type 2 immune response was initiated, whereas in spleen, a T helper cell type 1 immune response was observed. The stress has induced mainly upregulations of immune genes in the gill (cat-1, hep, il-10) and downregulations in the spleen (il-2, il-4/13a, il-8). Our results suggested that the unpredictable repeated stress protocol employed did not impair the fish immune system.

Neoparamoeba perurans , the aetiological agent of amoebic gill disease, remains a persistent threat to Atlantic salmon mariculture operations worldwide. Innovation in methods of AGD control is required yet constrained by a limited understanding of the mechanisms of amoebic gill disease pathogenesis. In the current study, a comparative transcriptome analysis of two N. perurans isolates of contrasting virulence phenotypes is presented using gill-associated, virulent (wild type) isolates, and in vitro cultured, avirulent (clonal) isolates. Differential gene expression analysis identified a total of 21,198 differentially expressed genes between the wild type and clonal isolates, with 5674 of these genes upregulated in wild type N. perurans . Gene set enrichment analysis predicted gene sets enriched in the wild type isolates including, although not limited to, cortical actin cytoskeleton, pseudopodia, phagocytosis, macropinocytic cup, and fatty acid beta-oxidation. Combined, the results from these analyses suggest that upregulated gene expression associated with lipid metabolism, oxidative stress response, protease activity, and cytoskeleton reorganisation is linked to pathogenicity in wild type N. perurans . These findings provide a foundation for future AGD research and the development of novel therapeutic and prophylactic AGD control measures for commercial aquaculture.

Amoebic Gill Disease (AGD) severely affects salmonid mariculture due to fish losses and costs associated with management of the disease. Continued research into management solutions, including new treatments and vaccine development, is highly important for the future of salmonid production worldwide. This requires both in vitro (both pathogen only and host-pathogen models) and in vivo (disease challenge) testing. Challenge models are still widely varied, in particular with regard to: infection methods (cohabitation or immersion), source of the pathogen (isolated from infected fish or cultured), infectious dose, environmental conditions (in particular temperature) and the endpoints across experimental treatment and vaccine studies which makes comparisons between studies difficult. This review summarises in vitro assays, the challenge methods and endpoints used in studies of experimental treatments and vaccines for AGD.