Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
4
result(s) for
"Offord, Alexander"
Sort by:
Communication inventions : the talk of the town
\"The ways in which people communicate has changed dramatically. The telephone to a cell phone, and mail to email are just some of the inventions examined in a progressive timeline of ways we communicate.\"--Amazon.com.
Book
First report of the invasive snail Pomacea canaliculata in Kenya
Following reports of an invasive snail causing crop damage in the expansive Mwea irrigation scheme in Kenya, samples of snails and associated egg masses were collected and sent to CABI laboratories in the UK for molecular identification. DNA barcoding analyses using the cytochrome oxidase subunit I gene gave preliminary identification of the snails as Pomacea canaliculata, widely considered to have the potential to be one of the most invasive invertebrates of waterways and irrigation systems worldwide and which is already causing issues throughout much of south-east Asia. To the best of our knowledge, this is the first documented record of P. canaliculata in Kenya, and the first confirmed record of an established population in continental Africa. This timely identification shows the benefit of molecular identification and the need for robust species identifications: even a curated sequence database such as that provided by the Barcoding of Life Data system may require additional checks on the veracity of the underlying identifications. We found that the egg mass tested gave an identical barcode sequence to the adult snails, allowing identifications to be made more rapidly. Part of the nuclear elongation factor 1 alpha gene was sequenced to confirm that the snail was P. canaliculata and not a P. canaliculata/P. maculata interspecies hybrid. Given the impact of this species in Asia, there is need for an assessment of the risk to Africa, and the implementation of an appropriate response in Kenya and elsewhere to manage this new threat to agriculture and the environment.
Journal Article
A compendium of synthetic lethal gene pairs defined by extensive combinatorial pan-cancer CRISPR screening
Background
Synthetic lethal interactions are attractive therapeutic candidates as they enable selective targeting of cancer cells in which somatic alterations have disrupted one member of a synthetic lethal gene pair while leaving normal tissues untouched, thus minimising off-target toxicity. Despite this potential, the number of well-established and validated synthetic lethal gene pairs is modest.
Results
We generate a dual-guide CRISPR/Cas9 Library and analyse 472 predicted synthetic lethal pairs in 27 cancer cell Lines from melanoma, pancreatic and lung cancer Lineages. We report a robust collection of 117 genetic interactions within and across cancer types and explore their candidacy as therapeutic targets. We show that SLC25A28 is an attractive target since its synthetic lethal paralog partner SLC25A37 is homozygously deleted pan-cancer. We generate knockout mice for
Slc25a28
revealing that, except for cataracts in some mice, these animals are normal; suggesting inhibition of SLC25A28 is unlikely to be associated with profound toxicity.
Conclusions
We provide and validate an extensive collection of synthetic lethal interactions across cancer types.
Journal Article