Explore the vast range of titles available.

Group A rotaviruses (RVAs) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. In rabbits, RVAs are associated with enteric disease, likely in combination with other pathogens. We report the identification and characterization of a lapine RVA strain in an Italian rabbit breeding farm. Increased mortality rates associated with enteric symptoms were reported in the facility in post-weaning rabbits around 40 days of age. By quantitative RT-PCR, an RVA strain was identified in the intestinal contents of deceased rabbits. A PCR-based enrichment protocol coupled with Nanopore sequencing allowed the reconstruction of the nearly complete genome of a rabbit RVA strain, Rabbit-wt/ITA/36-9/2022/G3P[14], with a genotype constellation (G3-P[14]-I2-R2-C2-M3-A9-N2-T6-E5-H3) conserved among lapine RVAs. Each of the 11 gene segments displayed high nucleotide identity and phylogenetic clustering with lapine rotavirus strains, as well as two Belgian human G3P[14] strains, which had been shown to have a zoonotic (lapine) origin. However, the NSP2 gene of strain 36-9 clustered closer with a group of rare human G3P[9] strains, suggesting a common path during their evolution. Gathering sequence data on animal RVAs is pivotal to reconstructing the history of homologous and heterologous RVAs in various mammals, including humans.

Background Implant placement in atrophic ridges presents a clinical challenge for surgeons and the results are often unpredictable. Static guided surgery is well-documented in cases where bone is adequate in height and width. However, its application with simultaneous augmentation procedures - such as ridge splitting or osteoperiosteal flap - remains underexplored. This study evaluated three-dimensional accuracy of static guided implant placement after guided ridge splitting or osteoperiosteal flap elevation, using a fully digital, dual-guide surgical protocol. Methods A total of 18 patients requiring horizontal ridge augmentation received 40 dental implants using a dual-guide static protocol. The first surgical guide was used for piezoelectric ridge splitting or osteoperiosteal flap elevation; the second allowed fully guided implant placement. Surgical guides were designed via cone-beam computed tomography (CBCT) and intraoral scan superimposition using BlueSkyPlan ® and in-office 3 Dimensional printing. Accuracy was assessed by comparing planned and actual implant positions on postoperative cone-beam computed tomography scans, measuring linear and vertical deviations at the entry point and apex, and angular deviation. Descriptive and inferential statistics were used to evaluate accuracy and subgroup differences. Results All 40 implants were successfully placed without complications. All implants achieved primary stability and had uneventful healing. Both approaches provided high placement accuracy comparable with values reported in the literature. Comparison between ridge splitting and osteoperiosteal flap techniques revealed no statistically significant differences across coronal, apical, and angular deviation parameters. The osteoperiosteal flap group showed numerically smaller deviations, but the difference was not statistically significant ( p  > 0.05). Conclusions A fully digital dual-guide static protocol enables accurate implant placement in augmented ridges using guided ridge splitting or osteoperiosteal flap techniques. This digital workflow may serve as a reliable alternative to conventional grafting methods in anatomically compromised sites. Trial registration This Randomized Clinical Trial has been retrospectively registered at Clinical Trials.gov with identification number: NCT07103577 on 2025-07-29.

Background Transient osteoporosis of the hip is a rare, self-limiting condition that typically affects middle-aged men and pregnant women in the third trimester. It is rarely observed in adolescents. Case report We present a unique case of transient osteoporosis of the hip in a 17-year-old Somali nonpregnant female who reported severe left hip pain and restricted mobility. Initial investigations revealed bone marrow edema on magnetic resonance imaging and deficiencies in phosphorus and magnesium. The patient was treated conservatively with bed rest, nutritional supplementation, bisphosphonates, and corticosteroids. This comprehensive approach led to significant improvement. At follow-up, the patient demonstrated complete resolution of symptoms and normalization of imaging findings. Conclusion This case underscores the importance of early diagnosis and targeted therapy in achieving full recovery from transient osteoporosis of the hip. Furthermore, it expands the demographic spectrum of transient osteoporosis of the hip, emphasizing its occurrence in nonpregnant adolescents.

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), emerged in Wuhan city (Hubei province, China) in December 2019, and the World Health Organization (WHO) declared an international public health emergency on 11 March 2020 [...]

The overuse and misuse of antibiotics can pose the risk of spreading mutant strains that show antimicrobial resistance (AMR), with negative impacts on the management of bacterial infections and economic implications for healthcare systems. The research and development of natural antibacterial agents could be a priority in the next years to improve a number of effective antibacterial molecules and to reduce the AMR phenomenon and its development. The present study identified the most effective concentration and contact time of Thymus vulgaris L. essential oil (TEO) to obtain bactericidal effects in vitro against different Gram-positive and Gram-negative bacterial strains. Six clinically isolated (wild types) bacterial strains, (Citrobacter freundii, Enterococcus feciorum, Proteus mirabilis, Acinetobacter cioffi, Pseudomonas putrefaciens and Klebsiella pneumoniae) and two ATCCs (Staphylococcus aureus and Streptococcus mutans) were tested after 1 min, 3 min and 5 min of contact with TEO. The preliminary results on S. aureus after 24 h of incubation revealed a TEO concentration of 9.28 mg/mL (w/v) that completely inhibited bacteria growth, keeping cell viability. The total suppression of bacterial growth at all tested contact times was observed for all tested bacterial strains, and the results were confirmed after 48 h of incubation. Bacterial growth suppression was confirmed even with the presence of organic components. These preliminary results showed the in vitro antimicrobial efficacy of TEO against different Gram-positive and Gram-negative bacterial strains. Future studies are necessary to confirm the reproducibility of these results even on other strains and to define the exact molecular mechanisms of EOs in order to consider TEO as a valid alternative to classic antibiotic therapies and subsequently to reduce the occurrence of AMR.

A 3-year-old female stray, shorthair cat, with clinical signs and serum chemistry markers indicative of hepatic disease, was diagnosed with domestic cat hepadnavirus (DCH) infection. Coupling molecular and serological data, the infection was seemingly contextualized into a chronic phase, since IgM anti-core antibodies, a marker of early-stage Hepatitis B Virus (HBV) infection, were not detected. However, the cat possessed IgG anti-core, a common indicator of chronic HBV infection in human patients and did not show seroconversion to the anti-DCH surface antigen, considered protective during HBV infection and associated with long-term protective immunity. On genome sequencing, the DCH strain showed 98.3% nucleotide identity to strains previously identified in Italy.

Pregnancy is a metabolically vulnerable state in which electrolyte disturbances, nutritional deficiencies, and hypoxic events can rapidly lead to severe neurological complications. Wernicke's encephalopathy and hypoxic-ischemic brain injury are uncommon but potentially devastating conditions that are frequently underdiagnosed, particularly in resource-limited intensive care settings. To our knowledge, the coexistence of profound hypokalemia, Wernicke's encephalopathy, and hypoxic-ischemic brain injury during pregnancy has been rarely reported, particularly from resource-limited intensive care settings. We report the case of a 22-year-old previously healthy pregnant woman at 18 weeks' gestation who presented with prolonged vomiting, progressive lower-limb weakness, and acute respiratory failure requiring mechanical ventilation. Laboratory evaluation revealed severe hypokalemia with associated acute kidney injury. Despite correction of electrolyte abnormalities, she developed persistent neuromuscular weakness, recurrent respiratory failure, and hemodynamic instability. Fluctuating mental status and ophthalmoplegia raised suspicion for Wernicke's encephalopathy, and high-dose parenteral thiamine was initiated. Brain magnetic resonance imaging later demonstrated hypoxic-ischemic changes. Her clinical course was further complicated by rhabdomyolysis, hepatic dysfunction, thrombocytopenia, and intrauterine fetal demise. Following prolonged ventilatory support and tracheostomy, she achieved partial neurological recovery and was discharged with ongoing rehabilitation needs. This case highlights the complex interplay between severe electrolyte disturbances, thiamine deficiency, and hypoxic brain injury during pregnancy. Early recognition of nutritional and metabolic causes of neurological deterioration, prompt empiric thiamine therapy, and aggressive prevention of hypoxic events are critical to improving maternal outcomes, particularly in resource-limited settings.

Introduction: Solid variant of aneurysmal bone cyst (SVABC) is a rare subtype of aneurysmal bone cyst (ABC) that presents as a solid, densely sclerotic lesion that can be more difficult to distinguish from other bone tumors and can lead to a wrong diagnosis. The SVABC rarely occurs in the long bones of the lower extremities. Case Presentation: In this report, we present a rare case of SVABC in a 25-year-old male patient, which was seen in the left fibula bone. The patient had a history of trauma for 7 years. A physical examination showed a non-tender swelling in his left fibula bone. A preoperative frontal radiograph showed a huge expansile lytic lesion with trabeculations in the proximal 2/3 of the left fibula. Magnetic Resonance Imaging (MRI) showed fibula with multiple cystic areas in the lesion, some containing fluid-fluid levels. Excision of the mass was performed. Histopathological examination of the surgical specimen of the left fibula mass confirmed that the lesion was SVABC and showed largely solid proliferation of mildly pleomorphic oval to spindle cells with giant cells. A postoperative frontal radiograph of the leg demonstrated proximal 2/3 of the left fibulectomy with no lesion recurrence. Conclusion: SVABC of the left fibula bone is a rare condition often misdiagnosed due to overlapping features with other aggressive bone lesions. Accurate diagnosis necessitates a multidisciplinary approach integrating clinical, imaging, and histopathology, with early surgical intervention being the gold standard for favorable outcomes. Surgeons must be cautious of postoperative complications like bleeding and neurological deficits, emphasizing the role of histopathology in preventing unnecessary surgeries. Future studies should focus on long-term follow-up and comparative treatment efficacy studies to enhance understanding and management of SVABC. Keywords: SVABC, bone, osteoclastic giant cells, radiology, excision

Introduction: Solid variant of aneurysmal bone cyst (SVABC) is a rare subtype of aneurysmal bone cyst (ABC) that presents as a solid, densely sclerotic lesion that can be more difficult to distinguish from other bone tumors and can lead to a wrong diagnosis. The SVABC rarely occurs in the long bones of the lower extremities. Case Presentation: In this report, we present a rare case of SVABC in a 25-year-old male patient, which was seen in the left fibula bone. The patient had a history of trauma for 7 years. A physical examination showed a non-tender swelling in his left fibula bone. A preoperative frontal radiograph showed a huge expansile lytic lesion with trabeculations in the proximal 2/3 of the left fibula. Magnetic Resonance Imaging (MRI) showed fibula with multiple cystic areas in the lesion, some containing fluid-fluid levels. Excision of the mass was performed. Histopathological examination of the surgical specimen of the left fibula mass confirmed that the lesion was SVABC and showed largely solid proliferation of mildly pleomorphic oval to spindle cells with giant cells. A postoperative frontal radiograph of the leg demonstrated proximal 2/3 of the left fibulectomy with no lesion recurrence. Conclusion: SVABC of the left fibula bone is a rare condition often misdiagnosed due to overlapping features with other aggressive bone lesions. Accurate diagnosis necessitates a multidisciplinary approach integrating clinical, imaging, and histopathology, with early surgical intervention being the gold standard for favorable outcomes. Surgeons must be cautious of postoperative complications like bleeding and neurological deficits, emphasizing the role of histopathology in preventing unnecessary surgeries. Future studies should focus on long-term follow-up and comparative treatment efficacy studies to enhance understanding and management of SVABC. Keywords: SVABC, bone, osteoclastic giant cells, radiology, excision

This study addresses the urgent need for culturally sensitive cognitive assessments in Egypt by validating an adapted version of Harmonized Cognitive Assessment Protocol (HCAP) to the Egyptian context. This version is to be used as a part of the Egyptian Aging Survey (AL-SEHA). We enrolled 300 participants aged 55 + from diverse backgrounds and meticulously adapted the HCAP for Egypt's linguistic and cultural context. Demonstrating strong reliability and validity (sensitivity 87.6%, specificity 89.2%, accuracy 89.7%), the Egyptian HCAP effectively identified cognitive impairment. Integrated into the AL-SEHA, this validated HCAP offers valuable insights on cognitive function decline in Egypt's aging population. Our findings not only contribute to global understanding of cognitive health but also set a precedent for future cross-cultural HCAP validations, informing policies and early diagnosis for dementia care.