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"Owings, Lisa"
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Learn about authors and illustrators
Just what does an author do? How about an illustrator? And who can do these interesting jobs? Find the answers to these questions and more in this book all about the people who create the books you read!
Book
Regulation of Paneth cell-specific genes in COVID-19 patients and SARS-CoV-2-infected mice by quantification of mRNA from exfoliated cells in stool samples
The Paneth cell, a secretory cell of the small intestine, expresses numerous host defense proteins, and is hypothesized to play an important role in host defense against infection. However, studying gene expression in this cell requires invasive procedures. To test the hypothesis that we could observe Paneth cell-specific gene regulation from exfoliated cells in infectious conditions, we obtained stool samples from patients with COVID-19 and uninfected controls. Total mRNA was isolated, and Paneth cell-specific and non-specific gene expression was quantified by RT-PCR. Results revealed a significant decrease in mRNA levels from Paneth cell-specific genes, including
DEFA5
,
DEFA6
,
PLA2G2A
,
PRSS2
and
ITLN2
in SARS-CoV-2 positive patients compared with controls. Other gut markers, not specific to Paneth cells were unchanged. To validate this experimentally, we infected mice with SARS-CoV-2 and collected fecal pellets over the course of 7 days. We observed a similar time-dependent reduction in Paneth cell-specific transcripts, which correlates with histological changes in the gut. This is the first demonstration of quantification of Paneth cell-specific transcripts without invasive sampling. It also shows the coordinate regulation of these genes as a response to infection with SARS-CoV-2, possibly through viral pathogenesis, to increase infectivity in the gut.
Journal Article
Bolivia
\"Developed by literacy experts for students in grades three through seven, this book introduces young readers to the geography and culture of Bolivia\"-- Provided by publisher.
Book
Geographic population structure analysis of worldwide human populations infers their biogeographical origins
The search for a method that utilizes biological information to predict humans’ place of origin has occupied scientists for millennia. Over the past four decades, scientists have employed genetic data in an effort to achieve this goal but with limited success. While biogeographical algorithms using next-generation sequencing data have achieved an accuracy of 700 km in Europe, they were inaccurate elsewhere. Here we describe the Geographic Population Structure (GPS) algorithm and demonstrate its accuracy with three data sets using 40,000–130,000 SNPs. GPS placed 83% of worldwide individuals in their country of origin. Applied to over 200 Sardinians villagers, GPS placed a quarter of them in their villages and most of the rest within 50 km of their villages. GPS’s accuracy and power to infer the biogeography of worldwide individuals down to their country or, in some cases, village, of origin, underscores the promise of admixture-based methods for biogeography and has ramifications for genetic ancestry testing.
Current methods to identify the geographical origin of humans based on DNA data present limited accuracy. Here, the authors develop a new algorithm, the Genographic Population Structure (GPS), and demonstrate its ability to place worldwide individuals within their country or, in some cases, village of origin.
Journal Article
The black mamba
\"Fascinating images accompany information about the black mamba. The combination of high-interest subject matter and narrative text is intended for students in grades 3 through 7\"--Provided by publisher.
Book
Correction: Corrigendum: Geographic population structure analysis of worldwide human populations infers their biogeographical origins
Nature Communications 5: Article number: 3513 (2014); Published: 29 April 2016; Updated: 31 October 2016 This article was published without any competing financial interests statement. A revised declaration that lists potentially competing financial interests is provided below: A.D.M., M.M. and M.A.are affiliated with Bcs Biotech S.
Journal Article
The great white shark
\"Fascinating images accompany information about the great white shark. The combination of high-interest subject matter and narrative text is intended for students in grades 3 through 7\"--Provided by publisher.
Book
Elephant seal
\"Engaging images accompany information about elephant seals. The combination of high-interest subject matter and narrative text is intended for students in grades 3 through 7\"-- Provided by publisher.
Book