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Hungary faces increasing water challenges, including frequent droughts and a growing dependence on irrigation, which necessitate alternative water sources for agriculture. This study evaluated the use of saline aquaculture effluent—characterized by elevated sodium (Na+) and chloride (Cl−) concentrations—as an irrigation resource for forage sorghum (Sorghum bicolor L.) over four consecutive growing seasons. Three hybrids (‘GK Áron’, ‘GK Balázs’, and ‘GK Erik’) were tested under five irrigation regimes, including freshwater and aquaculture effluent applied via drip irrigation at weekly doses of 30 mm and 45 mm, alongside a non-irrigated control. Effluent irrigation at 30 mm weekly increased biomass yield by up to 61% and enhanced nitrogen uptake by 22% compared to the control. Soil electrical conductivity (EC) values remained below 475 µS/cm, with effluent treatments showing lower EC than non-irrigated plots. The effluent water also supported the recycling of nutrients, especially nitrogen and phosphorus. Unlike conventional saline water, aquaculture effluent contains organic compounds and microbial activity that may improve nutrient mobilization and uptake. Our results highlight how we can reuse aquaculture wastewater in irrigated crop production. The results demonstrate that moderate effluent irrigation (30 mm/week) can optimize crop water use while maintaining soil health, offering a viable strategy for forage sorghum production in water-limited environments.

The growing global water shortage is an increasing challenge for the agricultural sector, which aims to produce sufficient quantity and quality of food and animal feed. In our study, effluent water from an intensive African catfish farm was irrigated on grain sorghum plants in four consecutive years. In our study the effects of the effluent on the N, P, K, Na content of the seeds, on the phenological parameters (plant height, relative chlorophyll content), the green mass, and on the grain yield of three varieties (‘Alföldi 1’, ‘Farmsugro 180’ and ‘GK Emese’) were investigated. Five treatments (Körös River (K) water and effluent (E) water: 30 and 45 mm weekly irrigation water dose; non-irrigated control) were applied with micro-spray irrigation. Compared to non-irrigated plants, effluent water did not negatively affect the N, P, K and Na contents of the grain crop. In terms of phenological parameters, the quality of the irrigation water (150–230 cm) had no negative effect on any of the measured parameters compared to the control (133–187 cm) values. In terms of biomass in 2020, grain yields were 89–109 g/plant with variety Alföldi 1, 64–91 g/plant with variety Farmsugro 180, and 86–110 g/plant with GK Emese.

At the core of the circadian network in Arabidopsis (Arabidopsis thaliana), clock genes/proteins form multiple transcriptional/translational negative feedback loops and generate a basic approximately 24-h oscillation, which provides daily regulation for a wide range of processes. This temporal organization enhances the fitness of plants only if it corresponds to the natural day/night cycles. Light, absorbed by photoreceptors, is the most effective signal in synchronizing the oscillator to environmental cycles. Phytochrome B (PHYB) is the major red/far-red light-absorbing phytochrome receptor in light-grown plants. Besides modulating the pace and phase of the circadian clock, PHYB controls photomorphogenesis and delays flowering. It has been demonstrated that the nuclear-localized amino-terminal domain of PHYB is capable of controlling photomorphogenesis and, partly, flowering. Here, we show (1) that PHYB derivatives containing 651 or 450 amino acid residues of the amino-terminal domains are functional in mediating red light signaling to the clock, (2) that circadian entrainment is a nuclear function of PHYB, and (3) that a 410-amino acid amino-terminal fragment does not possess any functions of PHYB due to impaired chromophore binding. However, we provide evidence that the carboxyl-terminal domain is required to mediate entrainment in white light, suggesting a role for this domain in integrating red and blue light signaling to the clock. Moreover, careful analysis of the circadian phenotype of phyB-9 indicates that PHYB provides light signaling for different regulatory loops of the circadian oscillator in a different manner, which results in an apparent decoupling of the loops in the absence of PHYB under specific light conditions.

In this study, more than 150 bacteria showing antagonistic properties against bacterial and fungal pathogens of the tomato plant were isolated and characterized. The most efficient agents against these phytopathogenic microorganisms belong to the genus Bacillus: the best biocontrol isolates were representatives of Bacillus subtilis, B. mojavensis and B. amyloliquefaciens species. They intensively produced fengycin or/and surfactin depsipeptide antibiotics and also proved to be excellent protease secretors. It was proved, that the selected strains were able to use ethylenethiourea (ETU) as sole nitrogen source. These antagonistic and ETU-degrading Bacillus strains can be applied as biocontrol and also as bioremediation agents.

Sorghum [Sorghum bicolor (L.) Moench] can benefit from accelerated breeding and release of improved varieties through doubled haploid technology. The technology has been used in speeding up the breeding of other major cereals such as wheat, maize and rice, for which generally widely applied optimised protocols exist. A reproducible protocol for the crop, that can overcome genotype dependency and other species-specific challenges such as phenolic exudation is however lacking. This study aimed at sorghum doubled haploids production thereby contributing to the development of an improved protocol. From the 28 hybrid genotypes, both F1 registered- and experimental hybrids involved, this study successfully produced haploids from five genotypes and subsequently, four confirmed doubled-haploid lines on W14mf medium or its modification with 1.0 gl−1 L-proline, 1.0 gl−1 L-asparagine and 1.0 gl−1 KH2PO4. Medium 190-2Cu was used for regeneration and rooting, which occurred successfully, if the calli were transferred on to it less than 7 days after induction, and temperature was maintained at 25˚C under light condition. Genotype dependency was not wholly overcome; however, sorghum’s high tillering ability and abiotic stress tolerance were observed to contribute to attainment of haploid plantlets. Spontaneous diploids producing seeds at rates of upto 80.5% were obtained, therefore eliminating the need for colchicine duplication.

Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although there was a genotype influence on the attainment of a yellow callus, friability of the callus was determined to be dependent on the culture medium and not the genotype. Half strength MS medium with 0.2 mg/l 2,4-D with 2.8 g/l Gelrite® as the gelling agent modified with 1.0 g/l KH2PO4, 1.0 g/l L-proline, 1.0 g/l L-asparagine and 0.16 mg/l CuSO4·5H2O (type E) was found to be the most effective resulting in about 60% yellow coloured callus induction with 25% friability. Addition of CuSO4·5H2O, KH2PO4, L-proline and L-asparagine significantly reduced the phenolic production. Half strength MS medium was observed to contribute to quality callus production when compared to full strength MS media modified with the compounds. The half strength MS medium was also observed to suppress phenolic production. Medium 190-2 produced the highest regeneration frequency (40%) among the 3-regeneration media tested. The results provide information on a suitable sorghum callus induction medium necessary for embryogenesis.

Commercial maize hybrids are exposed to different degrees of ear infection by toxigenic fungal species and toxin contamination. Their resistance to different fungi and toxin relationships are largely unknown. Without this knowledge, screening and breeding are not possible for these pathogens. Seven- to tenfold differences were found in resistance to Fusarium spp., and there was a five-fold difference in ear coverage (%) in response to A. flavus. Three hybrids of the twenty entries had lower infection severity compared with the general means for toxigenic species. Three were highly susceptible to each, and 14 hybrids reacted differently to the different fungi. Differences were also observed in the toxin content. Again, three hybrids had lower toxin content in response to all toxigenic species, one had higher values for all, and 16 had variable resistance levels. Correlations between infection severity and deoxynivalenol (DON) content were 0.95 and 0.82 (p = 0.001) for F. graminearum and F. culmorum, respectively. For fumonisin and F. verticillioides ear rot, the Pearson correlation coefficient (r) was 0.45 (p = 0.05). Two independent isolates with different aggressiveness were used, and their mean X values better described the resistance levels. This increased the reliability of the data. With the introduction of this methodological concept (testing the resistance levels separately for different fungi and with two isolates independently), highly significant resistance differences were found. The resistance to different fungal species correlated only in certain cases; thus, each should be tested separately. This is very useful in registration tests and post-registration screening and breeding. This would allow a rapid increase in food and feed safety.

Phenotypic and genotypic features of three teleomorphic species, Petromyces alliaceus, P. albertensis and P. muricatus and some related anamorphic Aspergillus species were compared. The dendrogram based on carbon source utilisation data revealed a close relationship between P. muricatus and the A. ochraceus strains examined. P. alliaceus and P. albertensis strains were very closely related to each other. A dendrogram with similar topology was obtained by analysing sequences of the intergenic transcribed spacer regions of representatives of these species. P. alliaceus and P. albertensis strains could only be distinguished by the random amplified polymorphic DNA technique. These strains possibly represent a single species closely related to Aspergillus section Flavi, while the anamorph of P. muricatus is a member of Aspergillus section Circumdati. Our results indicate that Aspergillus section Circumdati is in need of taxonomic revision.

Green fodder mixture trials were carried out with GK Impala, a winter hardy, fall sown oat variety registered in 2005 intercropped either with winter vetch or winter pea in a crop year when the spring was dry (2003) and in another one (2004) when the precipitation was optimal in spring. The two components of mixtures were sown 50% each. A four-replicate randomised complete block design was used with 50 m2 plots. The results were compared to the mixtures of spring oats and vetch; and spring oats and pea, respectively. The green matter of fall sown crops was cut by scythe late May, whereas that of spring crops early June.Data demonstrate that the green forage yield and protein production of fall sown oats as a monocrop and intercropped with vetch was higher than those of spring types. Green matter and dry matter yield varied with season, and were more advantageous in the year 2004, when there was more precipitation. The crude protein content of winter vetch and the crude protein production of the mixture fall sown oats + winter vetch were the highest. The mixtures with winter or spring pea yielded less green matter and, as a matter of fact, less crude protein. The fodder mixtures cereals — legumes are conventionally and widely used as feed for livestock in North-America, and the results of our two-year experiment suggest that their use should be intensified in Hungary as well, mainly in the provisional feeding of ruminants. To date, oat varieties with reliable winter hardiness are offered for fall sowing.

One of the most important effects of climate change is the occurrence of thermotolerant mycotoxin producing fungi in countries with temperate climate, causing mycotoxin contamination of agricultural products. Indeed, a shift has recently been observed in the occurrence of aflatoxin producing fungi in Europe, with consequent aflatoxin contamination in agricultural commodities including maize and milk in several European countries including Serbia, Croatia, Slovenia, Romania and Ukraine. These observations led us to examine the occurrence of mycotoxin producing Aspergilli in agricultural products in Hungary and Serbia. The samples were collected from cereal fields in Hungary and North-Serbia (Vojvodina) after harvest in 2012. Surface-sterilized cereal seeds were placed on selective media and the isolated fungal strains were identified using morphological methods. The species identification of selected isolates was carried out using sequence-based methods. Several potentially aflatoxigenic A. flavus isolates were identified on maize. Further examinations of mycotoxin producing abilities of the isolates, and their occurrence in milk and milk-derived products are in progress.