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11 result(s) for "Pandey, Satendra Kumar"
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Primary disseminated extrahepatic abdominal hydatid cyst: a rare disease
Hydatid disease (HD) is a parasitic disease, most frequently caused by Echinococcus granulosus or Echinococcus multilocularis and rarely by Echinococcus vogeli or Echinococcus oligarthus. Mostly, hydatid cyst is primarily in liver (75%) and lung (15%). Peritoneal HD (13%) is usually secondary. Dissemination of HD may occur following rupture of the hydatid cyst into the peritoneal cavity. Primary peritoneal hydatid cyst disease is a rare phenomenon. We present a case of primary disseminated abdominal HD presenting with the complaint of a pain in the epigastric region along with intermittent fever. Radiological evaluation and serological examination confirmed it as primary disseminated HD involving pancreas, a cyst anterior to the left lobe of the liver just below the peritoneum and the left kidney.
Primary disseminated extrahepatic abdominal hydatid cyst: a rare disease
Hydatid disease (HD) is a parasitic disease, most frequently caused by Echinococcus granulosus or Echinococcus multilocularis and rarely by Echinococcus vogeli or Echinococcus oligarthus. Mostly, hydatid cyst is primarily in liver (75%) and lung (15%). Peritoneal HD (13%) is usually secondary. Dissemination of HD may occur following rupture of the hydatid cyst into the peritoneal cavity. Primary peritoneal hydatid cyst disease is a rare phenomenon. We present a case of primary disseminated abdominal HD presenting with the complaint of a pain in the epigastric region along with intermittent fever. Radiological evaluation and serological examination confirmed it as primary disseminated HD involving pancreas, a cyst anterior to the left lobe of the liver just below the peritoneum and the left kidney.
Regulatory non-coding RNAs: a new frontier in regulation of plant biology
Beyond the most crucial roles of RNA molecules as a messenger, ribosomal, and transfer RNAs, the regulatory role of many non-coding RNAs (ncRNAs) in plant biology has been recognized. ncRNAs act as riboregulators by recognizing specific nucleic acid targets through homologous sequence interactions to regulate plant growth, development, and stress responses. Regulatory ncRNAs, ranging from small to long ncRNAs (lncRNAs), exert their control over a vast array of biological processes. Based on the mode of biogenesis and their function, ncRNAs evolved into different forms that include microRNAs (miRNAs), small interfering RNAs (siRNAs), miRNA variants (isomiRs), lncRNAs, circular RNAs (circRNAs), and derived ncRNAs. This article explains the different classes of ncRNAs and their role in plant development and stress responses. Furthermore, the applications of regulatory ncRNAs in crop improvement, targeting agriculturally important traits, have been discussed.
Systematic Investigation of Structural, Morphological and Optical Properties of Fe Doped ZnO Nanorods and Its Nanocomposite with Graphite Oxide
This study reports the synthesis, structural, morphological and optical properties of the semiconducting materials of pristine ZnO nanorods (NRs), Fe doped ZnO (Zn 1− x Fe x O) NRs, graphite oxide (GO) nanoparticles (NPs) and Zn 1− x Fe x O/GO nanocomposite (NCs) that may be promising candidate for semi-transparent solar cells and metamaterials based applications. The sol–gel method has been employed because of its ease and efficiency to synthesize NRs with nearly uniform sizes at room temperature. The hexagonal wurtzite structure and polycrystalline nature of ZnO based samples and hexagonal structure and crystalline nature of GO sample have been confirmed by x-ray diffraction (XRD). The occurrence of characteristic XRD peak at 23.85° and the absorption peak at 274 nm confirms the presence of GO in the nanocomposite material. In the absorbance spectra the maxima shifts from 377 to 366 nm as one goes from pristine ZnO NRs to Zn 1− x Fe x O/GO NCs material, which indicates a blue shift in the absorption edge. The optical band gap values using Tauc’s extrapolation method are 2.91, 2.97, 2.76 and 2.52 eV for pristine ZnO NRs, Fe doped ZnO NRs, GO NPs and Zn 1− x Fe x O/GO nanocomposite respectively. All the optical parameters, extinction coefficient ( k ) and refractive index ( n ) values have been calculated for the wavelength range of 200-800 nm using absorption and transmission spectra. Wemple–Di domenico single oscillator model is used in the calculation of static refractive index and optical bandgap of synthesized materials. The dielectric constant values show the epsilon negative material behaviour of synthesized samples, which can be employed in various metamaterials’ based applications.
Geospatial analysis of elephant habitat suitability and movement for mapping the elephant corridor in Dalma Wildlife Sanctuary (India)
Wildlife and natural resources constitute an integral part of the ecosystem, whereas human interventions dismantled the living conditions of the wildlife. This is testified in the Dalma Wildlife Sanctuary (DWS) where the habitats of Asian elephants have changed due to human intervention and deforestation over the decades. The present study aimed to assess the elephant habitat suitability in the DWS of Jharkhand state (India) using the geospatial parameters such as forest density, degree of slope, proximity to water bodies, land use land cover, proximity to agricultural land, built-up density, and road density. The analytical hierarchical process technique was utilized to determine habitat preference and selection of relevant factors to categorize criteria. The study revealed that about 6.7% (26.74 km 2 ) of the area is very highly suitable for elephant habitat, while 52.26% (208.49 km 2 ) of the forest area was found highly suitable. The most suitable habitat was identified in the core parts of the forest, while the least suitable areas were found in the southern part, where the presence of roads, built-up, and agricultural land was prominent. It was also observed that most human–elephant conflicts were exhibited in the low and very low suitable areas, while 90% of the elephant movement was witnessed in the high and very high suitable areas. Among the four identified corridors, three are inactive, and their location corresponds with low to very low suitable habitats. The study identified the migratory corridor routes inside the sanctuary where effective management is required for the conservation of elephant habitats and minimizing conflicts.
Editing cis‐elements of OsPHO1;2 improved phosphate transport and yield in rice
Summary Increasing grain yield is the primary goal of crop improvement, which is globally affected by the low availability of soil phosphate (Pi). Overexpressing Pi transporters to enhance Pi uptake often results in Pi toxicity and growth retardation. Despite advances in genetic engineering, targeting the cis‐regulatory motifs of Pi transporters remains underexplored for understanding plant mechanisms and improving Pi status. Here, we demonstrate that the excision of the transcription inhibitor motif from the promoter of the Pi transporter OsPHO1;2 enhances its expression and increases root‐to‐shoot Pi transport, leading to improved grain yield. Through in silico and DNA‐protein interaction studies, we show the role of the OsWRKY6 transcription factor in negatively regulating OsPHO1;2 expression by binding to the cis‐regulatory element (W‐box) present in its promoter. The oswrky6 knockout lines exhibit higher OsPHO1;2 expression and improved shoot Pi levels. Furthermore, we engineered the OsPHO1;2 promoter to precisely remove the W‐box and enhance OsPHO1;2 expression. Phenotypic and physiological evaluations at the vegetative stage indicate that OsPHO1;2 promoter‐edited (OsPHO1;2:PE) lines have increased shoot length, plant biomass and greater root‐to‐shoot Pi export under both low and normal P conditions. Notably, the 33P uptake assay reveals that OsPHO1;2:PE lines display enhanced root Pi uptake, supported by higher expression of root‐associated Pi transporters (OsPHTs). An extensive agronomic assessment shows that OsPHO1;2:PE lines achieve increased seed and panicle numbers, thereby raising yield without affecting seed quality. Our findings provide valuable insights into the potential of promoter editing to improve Pi use and enhance crop yield.
Crystallographic phases, phase transitions, and barrier layer formation in (1 − x) Pb(Fe1/2Nb1/2)O3−xPbTiO3
Powders of (1 − x) Pb(Fe1/2Nb1/2)O3-xPbTiO3 (PFN-PT) with x = 0.00, 0.10, 0.13, 0.15, 0.20, and 0.25 were prepared by the conventional solid-state route. Structure of PFN-PT was tetragonal for x [H33356] 0.10, which indicates that the morphotropic phase boundary (MPB) may be between 0 < x < 0.10. The nature of phase transition in PFN-PT changed from diffuse ferroelectric to relaxor ferroelectric to normal ferroelectric on increasing the PT content. The effect of the PT content and sintering temperature on barrier layer formation in the PFN-PT system was studied using complex impedance spectroscopy. With increasing PT content, the possibility of the barrier layer formation decreased while with increasing sintering temperature, the barrier layer formation was promoted.
Crystallographic phases, phase transitions, and barrier layer formation in (1 − x ) Pb(Fe 1/2 Nb 1/2 )O 3 − x PbTiO 3
Powders of (1 − x ) Pb(Fe 1/2 Nb 1/2 )O 3 - x PbTiO 3 (PFN-PT) with x = 0.00, 0.10, 0.13, 0.15, 0.20, and 0.25 were prepared by the conventional solid-state route. Structure of PFN-PT was tetragonal for x [H33356] 0.10, which indicates that the morphotropic phase boundary (MPB) may be between 0 < x < 0.10. The nature of phase transition in PFN-PT changed from diffuse ferroelectric to relaxor ferroelectric to normal ferroelectric on increasing the PT content. The effect of the PT content and sintering temperature on barrier layer formation in the PFN-PT system was studied using complex impedance spectroscopy. With increasing PT content, the possibility of the barrier layer formation decreased while with increasing sintering temperature, the barrier layer formation was promoted.
Editing cis-elements of OsPHO1;2 improved phosphate transport and yield in rice
Increasing grain yield is the main aim of crop improvement, which is globally impacted by the low availability of soil phosphate (Pi). Overexpressing Pi transporters for increasing Pi uptake often result in Pi toxicity and growth retardation. Despite advancements in genetic engineering, targeting cis-regulatory motifs of Pi transporters remains underexplored for understanding plant mechanisms and improving Pi status. Here, we demonstrated that the excision of the transcription inhibitor motif from the promoter of Pi transporter, OsPHO1;2, enhanced its expression and increased root-to-shoot Pi transport, leading to improved grain yield. Using, in-silico and DNA-protein interaction studies, we show the role of OsWRKY6 transcription factor in the negative regulation of OsPHO1;2 expression by binding to the cis-regulatory element (W-box) present in its promoter. oswrky6 knockout lines displayed a higher OsPHO1;2 expressions, and improved shoot Pi levels. Further, we engineered the OsPHO1;2 promoter for precisely removing the W-box and enhancing OsPHO1;2 expression. The phenotypic and physiological evaluation at the vegetative stage shows that OsPHO1;2 promoter edited (OsPHO1;2:PE) lines have increased shoot length, plant biomass and more root-shoot Pi export under low and normal P conditions. Notably, the 33Pi uptake assay reveals that OsPHO1;2:PE lines exhibited enhanced root Pi uptake, supported by higher root-associated Pi transporters (OsPHTs) expression. An extensive agronomical assessment revealed that OsPHO1;2:PE lines acquire increased seed and panicle numbers, thereby increasing yield without affecting seed quality. Our findings offer valuable insight into the potential of promoter editing to raise plants with better Pi use and improved crop yield.Competing Interest StatementThe authors have declared no competing interest.
Barrier layer formation and PTCR effect in (1-x) Pb(Fe1/2Nb1/2)O3-xPbTiO3 (x = 0.13) ceramics
(1-x)Pb(Fe1/2Nb1/2)O3-PbTiO3(PFN-xPT)Ceramics with x = 0.13 sintered at 1473K show diffuse phase transition and very high dielectric constant at lower frequencies.The high value of dielectric constant at lower frequencies is shown to be due to the barrier layer formation.The resistivity of the PFN-xPT ceramics, obtained by complex impedance analysis, initially decreases with temperature and then shows an upward trend beyond the ferroelectric Curie point reminiscent of BaTiO3 based thermistors with PTCR effect.