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Understanding what aspects of evolution are predictable, and repeatable, is a central goal of biology. Studying phenotypic convergence (the independent evolution of similar traits in different organisms) provides an opportunity to address evolutionary predictability at different hierarchical levels. Here we focus on recent advances in understanding the molecular basis of convergence. Understanding when, and why, similar molecular solutions are used repeatedly provides insight into the constraints that shape biological diversity. We first distinguish between convergence as a phenotypic pattern and parallelism as a shared molecular basis for convergence. We then address the overarching question: What factors influence when parallel molecular mechanisms will underlie phenotypic convergence? We present four core determinants of convergence (natural selection, phylogenetic history, population demography, and genetic constraints) and explore specific factors that influence the probability of molecular parallelism. Finally, we address frontiers for future study, including integration across different systems, subfields, and hierarchical levels.

The discovery of a radioactively powered kilonova associated with the binary neutron-star merger GW170817 remains the only confirmed electromagnetic counterpart to a gravitational-wave event 1 , 2 . Observations of the late-time electromagnetic emission, however, do not agree with the expectations from standard neutron-star merger models. Although the large measured ejecta mass 3 , 4 could be explained by a progenitor system that is asymmetric in terms of the stellar component masses (that is, with a mass ratio q of 0.7 to 0.8) 5 , the known Galactic population of merging double neutron-star systems (that is, those that will coalesce within billions of years or less) has until now consisted only of nearly equal-mass ( q  > 0.9) binaries 6 . The pulsar PSR J1913+1102 is a double system in a five-hour, low-eccentricity (0.09) orbit, with an orbital separation of 1.8 solar radii 7 , and the two neutron stars are predicted to coalesce in 470 − 11 + 12 million years owing to gravitational-wave emission. Here we report that the masses of the pulsar and the companion neutron star, as measured by a dedicated pulsar timing campaign, are 1.62 ± 0.03 and 1.27 ± 0.03 solar masses, respectively. With a measured mass ratio of q  = 0.78 ± 0.03, this is the most asymmetric merging system reported so far. On the basis of this detection, our population synthesis analysis implies that such asymmetric binaries represent between 2 and 30 per cent (90 per cent confidence) of the total population of merging binaries. The coalescence of a member of this population offers a possible explanation for the anomalous properties of GW170817, including the observed kilonova emission from that event. Pulsar timing measurements show a mass ratio of about 0.8 for the double neutron-star system PSR J1913+1102, and population synthesis models indicate that such asymmetric systems represent 2–30% of merging binaries.

Regional nutrient ranges are commonly used to diagnose plant nutrient status. In contrast, local diagnosis confronts unhealthy to healthy compositional entities in comparable surroundings. Robust local diagnosis requires well-documented data sets processed by machine learning and compositional methods. Our objective was to customize nutrient diagnosis of peach (Prunus persica) trees at local scale. We collected 472 observations from commercial orchards and fertilizer trials across eleven cultivars of Prunus persica and six rootstocks in the state of Rio Grande do Sul (RS), Brazil. The random forest classification model returned an area under curve exceeding 0.80 and classification accuracy of 80% about yield cutoff of 16 Mg ha-1 Centered log ratios (clr) of foliar defective compositions have appropriate geometry to compute Euclidean distances from closest successful compositions in 'enchanting islands'. Successful specimens closest to defective specimens as shown by Euclidean distance allow reaching trustful fruit yields using site-specific corrective measures. Comparing tissue composition of low-yielding orchards to that of the closest successful neighbors in two major Brazilian peach-producing regions, regional diagnosis diered from local diagnosis, indicating that regional standards may fail to fit local conditions. Local diagnosis requires well-documented Humboldtian data sets that can be acquired through ethical collaboration between researchers and stakeholders.

Over the past 20 years, the use of center-pivot irrigation has increased tomato ( L.) yields in Brazil from 42 Mg ha to more than 80 Mg ha . In the absence of field trials to support fertilizer recommendations, substantial amounts of phosphorus (P) have been applied to crops. Additional P dosing has been based on an equilibrated nutrient P budget adjusted for low-P fertilizer-use efficiency in high-P fixing tropical soils. To document nutrient requirements and prevent over-fertilization, tissue samples and crop yield data can be acquired through crop surveys and fertilizer trials. Nevertheless, most tissue diagnostic methods pose numerical difficulties that can be avoided by using the nutrient balance concept. The objectives of this study were to model the response of irrigated tomato crops to P fertilization in low- and high-P soils and to provide tissue diagnostic models for high crop yield. Three P trials, arranged in a randomized block design with six P treatments (0-437 kg P ha ) and three or four replications, were established on a low-P soil in 2013 and high-P soils in 2013 and 2014, totaling 66 plots in all. Together with crop yield data, 65 tissue samples were collected from tomato farms. We found no significant yield response to P fertilization, despite large differences in soil-test P (coefficient of variation, 24%). High- and low-yield classes (cutoff: 91 Mg fruits ha ) were classified by balance models with 78-81% accuracy using logit and Cate-Nelson partitioning models. The critical Mahalanobis distance for the partition was 5.31. Tomato yields were apparently not limited by P but were limited by calcium. There was no evidence that P fertilization should differ between center-pivot-irrigated and rain-fed crops. Use of the P budget method to arrive at the P requirement for tomato crops proved to be fallacious, as several nutrients should be rebalanced in Brazilian tomato cropping systems.

Remote oceanic islands have long been recognized as natural models for the study of evolutionary processes involved in diversification. Their remoteness provides opportunities for isolation and divergence of populations, which make islands remarkable settings for the study of diversification. Groups of islands may share a relatively similar geological history and comparable climate, but their inhabitants experience subtly different environments and have distinct evolutionary histories, offering the potential for comparative studies. A range of organisms have colonized the Galápagos Islands, and various lineages have radiated throughout the archipelago to form unique assemblages. This review pays particular attention to molecular phylogenetic studies of Galápagos terrestrial fauna. We find that most of the Galápagos terrestrial fauna have diversified in parallel to the geological formation of the islands. Lineages have occasionally diversified within islands, and the clearest cases occur in taxa with very low vagility and on large islands with diverse habitats. Ecology and habitat specialization appear to be critical in speciation both within and between islands. Although the number of phylogenetic studies is continuously increasing, studies of natural history, ecology, evolution and behaviour are essential to completely reveal how diversification proceeded on these islands.

High berry yield and quality of conventionally and organically grown cranberry stands require proper nutrient sources and dosage. Our objective was to model the response of cultivar “Stevens” to N, P, K, Mg, Cu, and B fertilization under conventional and organic farming systems. A 3-year trial was conducted on permanent plots at four production sites in Quebec, Canada. We analyzed yield predictors, marketable yield, and fruit quality in response to fertilization and soil properties. Cranberry responded primarily to nitrogen fertilization and, to a lesser extent, to potassium. Berry yield was closely related to the number of fruiting uprights (r = 0.92), berry counts per fruiting upright (r = 0.91), number of reproductive uprights (r = 0.83), and fruit set (r = 0.77). Nitrogen increased berry yield nonlinearly but decreased berry firmness, total anthocyanin content (TAcy), and total soluble solids content (°Brix) linearly, indicating a trade-off between berry yield and quality. Fertilizer dosage at a high-yield level ranged between 30 and 45 kg N ha−1 in both conventional and organic farming systems. Slow-release fertilizers delayed crop maturity and should thus be managed differently than ammonium sulfate. Berry weight increased with added K. Redundancy analysis showed a close correlation between marketable yield, berry quality indices, and soil tests, especially K and Ca, indicating the need for soil test calibration.

The classic evolutionary hypothesis of ecological opportunity proposes that both heterogeneity of resources and freedom from enemies promote phenotypic divergence as a response to increased niche availability. Although phenotypic divergence and speciation have often been inferred to be the primary consequences of the release from competition or predation that accompanies a shift to a new adaptive zone, increased phenotypic variation within species is expected to represent the first stage resulting from such a shift. Using measures of intraspecific morphological variation of 30 species of Galápagos endemic land snails in a phylogenetically controlled framework, we show that the number of local congeners and the number of local plant species are associated with lower and higher intraspecific phenotypic variation, respectively. In this clade, ecological opportunity thus explicitly links the role of competition from congeners and the heterogeneity of resources to the extent of intraspecific phenotypic divergence as adaptive radiation proceeds.

Background Calcareous outcrops, rocky areas composed of calcium carbonate (CaCO 3 ), often host a diverse, specialized, and threatened biomineralizing fauna. Despite the repeated evolution of physiological and morphological adaptations to colonize these mineral rich substrates, there is a lack of genomic resources for calcareous rock endemic species. This has hampered our ability to understand the genomic mechanisms underlying calcareous rock specialization and manage these threatened species. Results Here, we present a new draft genome assembly of the threatened limestone endemic land snail Oreohelix idahoensis and genome skim data for two other Oreohelix species. The O. idahoensis genome assembly (scaffold N50: 404.19 kb; 86.6% BUSCO genes) is the largest (~ 5.4 Gb) and most repetitive mollusc genome assembled to date (85.74% assembly size). The repetitive landscape was unusually dominated by an expansion of long terminal repeat (LTR) transposable elements (57.73% assembly size) which have shaped the evolution genome size, gene composition through retrotransposition of host genes, and ectopic recombination. Genome skims revealed repeat content is more than 2–3 fold higher in limestone endemic O. idahoensis compared to non-calcareous Oreohelix species. Gene family size analysis revealed stress and biomineralization genes have expanded significantly in the O. idahoensis genome . Conclusions Hundreds of threatened land snail species are endemic to calcareous rock regions but there are very few genomic resources available to guide their conservation or determine the genomic architecture underlying CaCO 3 resource specialization. Our study provides one of the first high quality draft genomes of a calcareous rock endemic land snail which will serve as a foundation for the conservation genomics of this threatened species and for other groups. The high proportion and activity of LTRs in the O. idahoensis genome is unprecedented in molluscan genomics and sheds new light how transposable element content can vary across molluscs. The genomic resources reported here will enable further studies of the genomic mechanisms underlying calcareous rock specialization and the evolution of transposable element content across molluscs.

Scar formation is a potentially detrimental process of tissue restoration in adults, affecting organ form and function. During fetal development, cutaneous wounds heal without inflammation or scarring at early stages of development; however, they begin to heal with significant inflammation and scarring as the skin becomes more mature. One possible cell type that could regulate the change from scarless to fibrotic healing is the mast cell. We show here that dermal mast cells in scarless wounds generated at embryonic day 15 (E15) are fewer in number, less mature, and do not degranulate in response to wounding as effectively as mast cells of fibrotic wounds made at embryonic day 18 (E18). Differences were also observed between cultured mast cells from E15 and E18 skin, with regard to degranulation and preformed cytokine levels. Injection of mast cell lysates into E15 wounds disrupted scarless healing, suggesting that mast cells interfere with scarless repair. Finally, wounds produced at E18, which normally heal with a scar, healed with significantly smaller scars in mast cell-deficient KitW/W-v mice compared with Kit+/+ littermates. Together, these data suggest that mast cells enhance scar formation, and that these cells may mediate the transition from scarless to fibrotic healing during fetal development.

Increasingly, multiple selective factors are recognized as jointly contributing to the evolution of morphology. What is not clear is how these forces vary across communities to promote morphological diversification among related species. In this study of Galápagos endemic snails (genus Naesiotus ), we test several hypotheses of colour evolution. We observe mockingbirds (genus Mimus ) predating live snails and find that avian predation selects against conspicuous shells. The evolutionary outcome of this selection is a diversity of shell colours across snails of the archipelago, each closely matching local backgrounds. We also find that snails more regularly exposed to the hot, equatorial sun reflect more light than shells of species from shadier habitats, suggesting a role for thermoregulatory constraints directing colour evolution. The signature of thermoregulatory selection is most clear in comparatively young communities (on the youngest islands), while the signature of selection from predators is most evident in older communities (on the older islands). Together, our findings point to a scenario of shifting selective forces along island ontogeny and community maturity that lead to the distribution of snail coloration we observe in Galápagos. Complex selective regimes such as these may have more responsibility for morphological diversity than is currently recognized.