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Apicomplexan parasites are auxotrophic for a range of amino acids which must be salvaged from their host cells, either through direct uptake or degradation of host proteins. Here, we describe a family of plasma membrane-localized amino acid transporters, termed the Apicomplexan Amino acid Transporters (ApiATs), that are ubiquitous in apicomplexan parasites. Functional characterization of the ApiATs of Toxoplasma gondii indicate that several of these transporters are important for intracellular growth of the tachyzoite stage of the parasite, which is responsible for acute infections. We demonstrate that the ApiAT protein TgApiAT5-3 is an exchanger for aromatic and large neutral amino acids, with particular importance for L-tyrosine scavenging and amino acid homeostasis, and that TgApiAT5-3 is critical for parasite virulence. Our data indicate that T. gondii expresses additional proteins involved in the uptake of aromatic amino acids, and we present a model for the uptake and homeostasis of these amino acids. Our findings identify a family of amino acid transporters in apicomplexans, and highlight the importance of amino acid scavenging for the biology of this important phylum of intracellular parasites.

Background Incorporating cycling into daily life is one way to increase physical activity. Purpose This study examined the impact of building new bike lanes in New Orleans to determine whether more people were cycling on the street and with the flow of traffic after bike lanes were built. Methods Through direct observation of one intervention and two adjacent streets, observers counted cyclists riding on the street and sidewalk, with and against traffic, before and after installation of the lanes. Data were tallied separately for adults, children, males, females, and by race for each location. Results There was an increase in cyclists on all three streets after the installation of the bike lanes, with the largest increase on the street with the new lane. Additionally, the proportion of riders cycling with traffic increased after the lanes were striped. Conclusions Bike lanes can have a positive impact in creating a healthy neighborhood.

ObjectivesThe interim Foundation Year 1 (FiY1) post was created in response to the COVID-19 pandemic to help bolster the workforce and manage increased clinical pressures. This study aimed to assess the impact of the FiY1 post on medical graduates’ self-reported confidence in common tasks, core skills, competencies and procedures prior to starting FY1, as a measure of increasing preparedness for practice.SettingA longitudinal survey was performed at a tertiary teaching hospital in the South East of England. FiY1 posts ran from June to July 2020.ParticipantsQuestionnaires were sent to 122 medical graduates from a single medical school (recipients included FiY1s and non-FiY1s) and to 69 FiY1s at a single Teaching Hospital NHS Trust, irrespective of medical school attended. Initial and follow-up questionnaires had 86 and 62 respondents, respectively. Of these, 39 graduates were matched; 26 were FiY1s and 13 non-FiY1s. The 39 matched results were analysed.Primary outcome measuresConfidence levels in common FY1 tasks, core procedures and competencies were gathered before and after the FiY1 post through online questionnaires. Change in confidence comparing FiY1s and non-FiY1s was measured and analysed using linear regression.ResultsOn a 5-point scale, the FiY1 post increased overall confidence in starting FY1 by 0.62 (95% CI 0.072 to 1.167, p=0.028). The FiY1 post increased confidence in performing venepuncture by 0.32 (95% CI 0.011 to 0.920, p=0.045), performing intravenous cannulation by 0.48 (95% CI 0.030 to 1.294, p=0.041) and recognising, assessing and initiating the management of the acutely ill patient by 0.32 (95% CI 0.030 to 1.301, p=0.041).ConclusionsThe COVID-19 pandemic FiY1 post improved confidence in core skills and competencies. These findings may help guide future educational interventions in conjunction with further larger scale studies, ultimately aiding to bridge the transition gap between being a medical student and a doctor.

Shakespeare's 'Twelfth Night' contains in its title an affinity with the festive season of Christmas, which in early modern England spanned for twelve days from 25 December through to 'Twelfth Night' on 6 January. The early performance history of 'Twelfth Night' includes accounts of private performances at Whitehall, aristocratic households and the Blackfriars playhouse, where the King's Men performed during the winter months from 1609 following. These performances did not necessarily occur during the Christmas festivities, with some examples of the play being performed on Candlemas and Easter Monday. This could explain the ambiguous subtitle of the play, 'Or What You Will', which seems to imply that if not on Twelfth Night, the play could be performed whenever it suited Shakespeare's playing company to do so. The play's plot, characters and setting also appear to have little or no connection to traditional festivities of Twelfth Night. The relevance of this occasion must therefore lie in other elements of the play. Tiffany Stern has deemed the most plausible connection to be in the play's characteristically 'musical' quality, which reflects the prevalence of masques and musical festivities in private households on Twelfth Night. In this article, I expand this view to illustrate how the communal, participatory style of the music for Wassailing, a Saturnalian festival celebrated on Twelfth Night, is reflected in one lengthy scene in the middle of the play.

Background In 2019, our interdisciplinary team of researchers, family members, and youth co-designed four simulation training videos and accompanying facilitation resources to prepare youth, family members, trainees, and researchers to build the knowledge and skills to engage in patient-oriented research (POR) authentically and meaningfully. Videos covered challenges in aspects of the research process including (1) forming a project team; (2) identifying project objectives and priorities; (3) agreeing on results; and (4) carrying out knowledge translation. Methods The purpose of the study was to deliver four simulation training videos across 2 two-hour facilitated workshops with researchers, trainees, and family partners. We evaluated whether the training videos and facilitated discussion of the simulations helped to improve knowledge and attitudes about authentic and meaningful partnership in research and self-perceived ability to engage in POR. An explanatory sequential two-phase mixed methods design was used. Phase 1 (quantitative) included two training workshops and a pre/post-training survey. Phase 2 (qualitative) included two qualitative focus groups. Results of each phase were analyzed separately and then combined during interpretation. Results Sixteen individuals (including researchers/research staff, trainees, family members, clinicians) took part in this research study. Overall, participants were highly receptive to the training, providing high scores on measures of acceptability, appropriateness, and feasibility. While the training videos and facilitated discussion of the simulations were found to increase participants’ knowledge and ability to engage in authentic and meaningful POR, we found no significant change in attitude or intent. Recommendations about the simulation content and delivery were provided to inform for future use. Conclusions The simulations were found to be a positive and impactful way for collaborative research teams to build knowledge and ability to engage in authentic and meaningful POR. Recommendations for future work include covering different content areas with varying levels of nuance; and offering the training to stakeholders in a variety of roles, such as those higher-ranked academic positions. Plain English summary In 2019, our team of researchers, family members, and youth worked together to design and develop four digitally recorded simulation videos that can be used to train youth, caregivers/families, trainees, and researchers to engage with each other in research so that all parties feel supported and valued. This paper describes how the four simulation videos were packaged in the training and then delivered to 16 participants (researchers, trainees, and caregivers/families). We used multiple ways to evaluate the videos and training, including a survey before and after the training, focus groups with participants after the training, and written reflections shared by the training facilitators after the training was finished. We found that the simulation videos increased participants’ knowledge on engagement and their self-reported ability to engage in authentic and meaningful patient-oriented research. Participants rated their belief in engagement and their intent to engage in collaborative research highly at the pre-test and this remained consistent at the post-test. Participants liked that the simulations focused on challenges in research engagement and that the training was offered to researchers and family partners together. They provided valuable feedback on what we should change about the simulations, including the content, which should have less exaggerated lessons and to add more topics. They also suggested it would be helpful if stakeholders other than just the research team complete the training in the future, especially those who are in higher positions of academic power.

Public health obesity prevention experts have recently emphasized a policy systems and environmental change approach. Absent, however, are studies describing how practitioners transition from policy adoption to implementation. In the realm of food policy, financing programs to incentivize healthy food retail development in communities classified as “underserved” are underway at the local, state, and national levels. Implementing these policies requires a clear definition of eligibility for program applicants and policy administrators. This article outlines a methodology to establish eligibility for healthy food financing programs by describing the work of The Food Trust to coadminister programs in 3 distinct regions. To determine program eligibility, qualitative assessments of community fit are needed and national data sources must be locally verified. Our findings have broad implications for programs that assess need to allocate limited public/private financing resources.

Aims To characterise continuous glucose monitor and flash glucose monitor use and related issues (both positive and negative) in the population of children and young people living with type 1 diabetes in the Southern Health and Social Care Trust (SHSCT), Northern Ireland. Methods Four focus groups were conducted in the SHSCT with people with type 1 diabetes and their families, to assess opinions of diabetes technology. In addition, questionnaires were distributed to diabetes specialist nurses (DSNs), young people and their families about use and access to diabetes technology. Results Questionnaires were completed by 68 children, young people (0–18 years) and their parents/guardians. Nearly all (98.5%) had access to their own diabetes related data, and 70.6% used diabetes data systems, for example, Libreview. Most of those using these systems found them beneficial and easy for daily use and clinical review. Most DSNs (83.9%) agreed that the systems were easy to use, and 82.3% were confident in interpreting the data accurately. DSNs felt virtual review was beneficial in over half of young people with 62.9% advising changes to diabetes management based on the data. Focus groups participants deemed diabetes technology a ‘gamechanger’ and ‘lifechanging’. Some drawbacks included ‘disruptive’ alarms, self‐confidence issues, visible diabetes technology and problems in school. Conclusions Most young people, their families and healthcare professionals reported they were confident in interpreting diabetes data and technology was easy to use. Diabetes technology could improve access to care through virtual clinics, improve clinical outcomes and enhance quality of life.

In the 8th to 10th century c.e., six adult individuals, five males and one female, were murdered in the northern Badia of Jordan amongst the ruins of Qasr Hallabat. The four males and one female show a combination of blunt force trauma to the head and sharp force trauma to the arms and legs. Who are these individuals, and what are the circumstances surrounding their deaths? Forensic and bioarchaeological analyses of the skeletal remains uncover evidence surrounding this crime and how these individuals may have ended up meeting their deaths at Hallabat.

More than three decades of systematic archaeological research in the American Bottom region of southwestern Illinois have produced a continuous flow of new archaeobotanical information. Pioneering syntheses by Sissel Johannessen (1984 and 1988) offered the first insights into rich and complex relationships between prehistoric humans and plants in this region. Since that time, our appreciation for the depth and intricacy of prehistoric plant use strategies has grown along with the increasing specificity of new geographic and chronological botanical data. However, along with an enhanced appreciation, there is also the realization that some of the previous models of prehistoric cultural continuity were in error. Pre-Late Woodland Period occupations in the American Bottom area, especially, are best understood as a series of pulses rather than products of in situ cultural evolution. The non-continuous model has proven helpful in assessing apparent anomalies or inconsistencies in early prehistoric, Archaic through Middle Woodland, plant use patterns. Understanding of post-Late Woodland adaptive strategies has benefited generally from the sheer wealth of data, and also from greater attention given to context of that data. Although subsistence must continue to be a central focus of interpretive efforts, plant remains have also proven useful for examining broad issues of prehistoric economics, technology and ritual. In this paper, we present a revised synthesis of American Bottom archaeobotanical data, explore the revisions in terms of previous interpretations, and outline some ideas for new directions.