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result(s) for
"Parraga, Katheryn"
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Insects as Valuable Sources of Protein and Peptides: Production, Functional Properties, and Challenges
by
Hasnan, Fatin Fayuni Binti
,
Feng, Yiming
,
Parraga, Katheryn
in
absorption
,
Agribusiness
,
Agricultural practices
2023
As the global population approaches 10 billion by 2050, the critical need to ensure food security becomes increasingly pronounced. In response to the urgent problems posed by global population growth, our study adds to the growing body of knowledge in the field of alternative proteins, entomophagy, insect-based bioactive proteolysates, and peptides. It also provides novel insights with essential outcomes for guaranteeing a safe and sustainable food supply in the face of rising global population demands. These results offer insightful information to researchers and policymakers tackling the intricate relationship between population expansion and food supplies. Unfortunately, conventional agricultural practices are proving insufficient in meeting these demands. Pursuing alternative proteins and eco-friendly food production methods has gained urgency, embracing plant-based proteins, cultivated meat, fermentation, and precision agriculture. In this context, insect farming emerges as a promising strategy to upcycle agri-food waste into nutritious protein and fat, meeting diverse nutritional needs sustainably. A thorough analysis was conducted to evaluate the viability of insect farming, investigate insect nutrition, and review the techniques and functional properties of protein isolation. A review of peptide generation from insects was conducted, covering issues related to hydrolysate production, protein extraction, and peptide identification. The study addresses the nutritional value and global entomophagy habits to elucidate the potential of insects as sources of peptides and protein. This inquiry covers protein and hydrolysate production, highlighting techniques and bioactive peptides. Functional properties of insect proteins’ solubility, emulsification, foaming, gelation, water-holding, and oil absorption are investigated. Furthermore, sensory aspects of insect-fortified foods as well as challenges, including Halal and Kosher considerations, are explored across applications. Our review underscores insects’ promise as sustainable protein and peptide contributors, offering recommendations for further research to unlock their full potential.
Journal Article
Effect of Enhanced Antimicrobial Fish Gelatin and Lactic Acid Coating on the Shelf Life of Fresh and Previously Frozen Shrimp
2025
Gelatin coatings have been shown to successfully slow the oxidation of fresh foods. Furthermore, organic acids have proven effective in the inhibition of bacteria and the extension of product shelf life. The purpose of this study was to observe the effects of gelatin and lactic acid treatment combinations on fresh and previously frozen (thawed) wild-caught shrimp. The samples were separated into four treatment groups: control (C), gelatin coating (G), lactic acid followed by gelatin coating (L), and gelatin infused with lactic acid coating (LG). Half of each group was stored at refrigeration temperature (≤4 °C), while the other half was frozen (≤−18 °C) and thawed for the thawed study. Physical, chemical, and microbiological changes were observed in refrigeration storage for 8 days. Results showed that fresh and thawed shrimp exhibited darkening in color over time. Treatments with lactic acid were more blue than yellow. Treated samples developed less oxidation than the control. Psychrophilic counts for samples L and LG remained below the 7 log CFU/g threshold throughout the study. In fresh shrimp samples, the addition of just a gelatin coating was able to increase shelf life by three days, while the addition of lactic acid successfully lowered microbial counts and extended the shelf life by six days. The freeze–thaw cycle negatively affected the extension of shelf life in the G group.
Journal Article
Evaluation of ultraviolet (UV‐C) light treatment for microbial inactivation in agricultural waters with different levels of turbidity
by
Parraga Estrada, Katheryn J.
,
Adhikari, Achyut
,
Chhetri, Vijay S.
in
Deactivation
,
Disinfection
,
Drip irrigation
2020
Produce growers using surface or well water to irrigate their crops may require an appropriate water treatment system in place to meet the water quality standard imposed by FSMA Produce Safety Rule. This study evaluated the potential of using ultraviolet (UV‐C) treatment in reducing the microbial population in agricultural water. Waters with turbidity levels ranging from 10.93 to 23.32 Nephelometric Turbidity Units (NTU) were prepared by mixing pond water and well water. The waters were inoculated with a cocktail of generic Escherichia coli (ATCC 23716, 25922, and 11775) and then treated with UV‐C light (20–60 mJ/cm2). All tested doses of the UV‐C treatment reduced the E. coli levels significantly (p < .05) in the water samples with the turbidity levels up to 23.32 NTU. The decrease in the turbidity from 23.32 to 10.93 NTU increased the level of reduction by more than 2.15 log most probable number (MPN)/100 ml). UV‐C treatment effectively reduces microbial load in agriculture water; however, turbidity of water may significantly affect the disinfection efficacy. The study also demonstrated that sprinkler system resulted in a higher level of contamination of cantaloupes compared with drip irrigation. The results indicated that UV‐C treatment could be a promising strategy in reducing the produce safety risks associated with irrigation water. This study evaluated the potential of using ultraviolet (UV‐C) treatment in reducing the microbial population in agricultural water. All tested doses of the UV‐C treatment reduced the E. coli levels significantly (p < .05) in the water samples with the turbidity levels up to 23.32 NTU. The decrease in the turbidity from 23.32 to 10.93 NTU increased the level of reduction by more than 2.15 log most probable number (MPN)/100 ml). UV‐C treatment effectively reduces microbial load in agriculture water; however, turbidity of water may significantly affect the disinfection efficacy.
Journal Article
Controlling foodborne pathogens in irrigation water: the effectiveness of zeolite modified with cetrimonium bromide
by
Janes, Marlene E.
,
Adhikari, Achyut
,
Fontenot, Kathryn
in
Cetyltrimethylammonium bromide
,
Disease control
,
E coli
2024
Recurring foodborne outbreaks, attributed to Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes, have identified irrigation water as a potential source of contamination, and creating the necessity for safe irrigation water in produce cultivation, as emphasized by the Food Safety Modernization Act (FSMA). In response to this imperative, this study explored the efficacy of surfactant-modified zeolite (SMZ) enhanced with Cetrimonium bromide (CTAB) as a sustainable water purification solution for surface water. The SMZ was assessed to have the capacity to filter contaminated water with high loads of foodborne pathogens. A laboratory study was conducted using a 100 g SMZ column. A liter of phosphate-buffered saline (PBS) was inoculated for each pathogen at 6 log CFU mL-1 concentrations. The study found that SMZ modified with CTAB at a concentration exceeding 20% by weight, indicating the ratio of CTAB to the total mass of the modifying solution, could eliminate >6 log CFU mL-1 of Escherichia coli O157:H7 and Listeria monocytogenes and >2 log of Salmonella sp. Subsequent field testing in strawberry farms demonstrated the system’s effectiveness, displaying significant bacterial reduction when contrasted with unfiltered pond water and sand filtration. The SMZ was able to filter more than 4 log CFU mL-1, from surface irrigation water spiked with a nonpathogenic Escherichia coli strain. The results indicate that the SMZ filtration approach holds promise as a remediation tool to control the risks of foodborne disease outbreaks associated with agricultural water.
Journal Article
Effect of Federal Inspection on Louisiana Wild-Caught Catfish Industry, Prevalence of Salmonella, and Microbial Characteristics of Raw Wild-Caught Catfish Fillets
2021
The United States is one of the largest catfish producers in the world. Louisiana is the leading producer of wild-caught catfish. Historically, the U.S. Food and Drug Administration inspected all seafood products; however, in 2008, Congress moved the inspection of fish in the order Siluriformes to the U.S. Department of Agriculture (USDA), Food Safety and Inspection Service. Full enforcement of the rule began on 1 September 2017. The present study was conducted to assess the impact of USDA Siluriformes fish regulation on small Louisiana wild-caught catfish processors and to determine the microbiological quality of and Salmonella prevalence in raw fillets. Nine facilities participated in the assessment study. Surveys were conducted before and after full enforcement to identify whether facilities had established prerequisite programs and record keeping associated with sanitation, hazard analysis and critical control point (HACCP) plans, food defense, and product recall. The processors' attitude about the change in regulations also was analyzed. For analysis of the microbiological quality and Salmonella prevalence, catfish samples were collected once per month for 2 years. Samples were evaluated for aerobic bacteria counts (APC), coliforms, Escherichia coli, Staphylococcus aureus, and Salmonella. The preenforcement survey revealed that only one facility had developed a HACCP plan, but it was not implemented. After 1 year of full enforcement, all the facilities developed and implemented a HACCP plan to process fresh catfish, and 78% of the processors reported a reduction in the amount of catfish processed due to limits in hours of operation and loss of fishermen. For microbiological quality, the mean (±SD) APC and counts of E. coli, coliforms, and S. aureus were 5.01 ± 0.70, 0.58 ± 0.89, 2.16 ± 0.77, and 0.73 ± 1.02 log CFU/g, respectively; 5.3% of the samples was confirmed positive for Salmonella. These findings indicate that after USDA enforcement, facilities improved food safety program documentation; however, the processing practices did not change. The microbial quality of the catfish fillets was within the acceptable levels in accordance with the International Commission on Microbiological Specifications for Foods.
Journal Article