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The spontaneous activity of cortical networks is characterized by the emergence of different dynamic states. Although several attempts were accomplished to understand the origin of these dynamics, the underlying factors continue to be elusive. In this work, we specifically investigated the interplay between network topology and spontaneous dynamics within the framework of self-organized criticality (SOC). The obtained results support the hypothesis that the emergence of critical states occurs in specific complex network topologies. By combining multi-electrode recordings of spontaneous activity of in vitro cortical assemblies with theoretical models, we demonstrate that different ‘connectivity rules’ drive the network towards different dynamic states. In particular, scale-free architectures with different degree of small-worldness account better for the variability observed in experimental data, giving rise to different dynamic states. Moreover, in relationship with the balance between excitation and inhibition and percentage of inhibitory hubs, the simulated cortical networks fall in a critical regime.

Sustainable manufacturing is gaining attention in the scientific literature. However, it remains unclear how to effectively incorporate it within Production Planning and Control (PPC) tasks. All the choices taken in terms of PPC impact sustainability, and sustainability managers and planners or managers involved in tasks, such as scheduling or inventory management, are not conscious of what this means or implies, above all, in terms of the sustainable performance indicators on which their actions can act. While several studies have addressed both PPC and sustainability, there is still limited guidance or structured frameworks specifically aimed at systematically linking PPC tasks with sustainability indicators in a practical and operational industrial context, despite the development of numerous sustainability standards in recent years. For this reason, this research aimed to develop a first detailed framework, specifically based on the Global Reporting Initiative (GRI) standard, that associates the most relevant indicators with the PPC phases, highlighting the type of impact (direct or indirect) of each phase on them. This could help with strategic decisions and promote more informed choices. The overall framework revealed the prevalence of environmental aspects involved in PPC phases (as expected) and a challenge related to the measurability of indicators (above all, the social ones). Furthermore, the Material Requirements Planning (MRP), identified as the most significant phase in terms of its impact on sustainability, was deeply analyzed, providing details related to the decision-making processes of this phase that affect sustainable performance.

Uniform and modular primary hippocampal cultures from embryonic rats were grown on commercially available micro-electrode arrays to investigate network activity with respect to development and integration of different neuronal populations. Modular networks consisting of two confined active and inter-connected sub-populations of neurons were realized by means of bi-compartmental polydimethylsiloxane structures. Spontaneous activity in both uniform and modular cultures was periodically monitored, from three up to eight weeks after plating. Compared to uniform cultures and despite lower cellular density, modular networks interestingly showed higher firing rates at earlier developmental stages, and network-wide firing and bursting statistics were less variable over time. Although globally less correlated than uniform cultures, modular networks exhibited also higher intra-cluster than inter-cluster correlations, thus demonstrating that segregation and integration of activity coexisted in this simple yet powerful in vitro model. Finally, the peculiar synchronized bursting activity shown by confined modular networks preferentially propagated within one of the two compartments ('dominant'), even in cases of perfect balance of firing rate between the two sub-populations. This dominance was generally maintained during the entire monitored developmental frame, thus suggesting that the implementation of this hierarchy arose from early network development.

Cultures of dissociated cortical neurons represent a powerful trade-off between more realistic experimental models and abstract modeling approaches, allowing to investigate mechanisms of synchronized activity generation. These networks spontaneously alternate periods of high activity (i.e. network bursts) with periods of quiescence in a dynamic state which recalls the fluctuation of in vivo UP and DOWN states. Network bursts can also be elicited by external stimulation and their spatial propagation patterns tracked by means of multi-channel micro-electrode arrays. In this study, we used rat cortical cultures coupled to micro-electrode arrays to investigate the similarity between spontaneous and evoked activity patterns. We performed experiments by applying electrical stimulation to different network locations and demonstrated that the rank orders of electrodes during evoked and spontaneous events are remarkably similar independently from the stimulation source. We linked this result to the capability of stimulation to evoke firing in highly active and “leader” sites of the network, reliably and rapidly recruited within both spontaneous and evoked bursts. Our study provides the first evidence that spontaneous and evoked activity similarity is reliably observed also in dissociated cortical networks.

Large scale transitions between active (up) and silent (down) states during quiet wakefulness or NREM sleep regulate fundamental cortical functions and are known to involve both excitatory and inhibitory cells. However, if and how inhibition regulates these activity transitions is unclear. Using fluorescence-targeted electrophysiological recording and cell-specific optogenetic manipulation in both anesthetized and non-anesthetized mice, we found that two major classes of interneurons, the parvalbumin and the somatostatin positive cells, tightly control both up-to-down and down-to-up state transitions. Inhibitory regulation of state transition was observed under both natural and optogenetically-evoked conditions. Moreover, perturbative optogenetic experiments revealed that the inhibitory control of state transition was interneuron-type specific. Finally, local manipulation of small ensembles of interneurons affected cortical populations millimetres away from the modulated region. Together, these results demonstrate that inhibition potently gates transitions between cortical activity states, and reveal the cellular mechanisms by which local inhibitory microcircuits regulate state transitions at the mesoscale.

Predicting workload for tasks in manufacturing is a complex challenge due to the numerous variables involved. In small- and medium-sized enterprises (SMEs), this process is often experience-based, leading to inaccurate predictions that significantly impact production planning, order management, and consequently the ability to meet customer deadlines. This paper presents an approach that leverages machine learning to enhance workload prediction with minimal data collection, making it particularly suitable for SMEs. A case study application using supervised machine learning models for regression, trained in an open-source data analytics, reporting, and integration platform (KNIME Analytics Platform), has been carried out. An Automated Machine Learning (AutoML) regression approach was employed to identify the most suitable model for task workload prediction based on minimising the Mean Absolute Error (MAE) scores. Specifically, the Regression Tree (RT) model demonstrated superior accuracy compared to more traditional simple averaging and manual predictions when modelling data for a single product type. When incorporating all available product data, despite a slight performance decrease, the XGBoost Tree Ensemble still outperformed the traditional approaches. These findings highlight the potential of machine learning to improve workload forecasting in manufacturing, offering a practical and easily implementable solution for SMEs.

The state of open science needs to be monitored to track changes over time and identify areas to create interventions to drive improvements. In order to monitor open science practices, they first need to be well defined and operationalized. To reach consensus on what open science practices to monitor at biomedical research institutions, we conducted a modified 3-round Delphi study. Participants were research administrators, researchers, specialists in dedicated open science roles, and librarians. In rounds 1 and 2, participants completed an online survey evaluating a set of potential open science practices, and for round 3, we hosted two half-day virtual meetings to discuss and vote on items that had not reached consensus. Ultimately, participants reached consensus on 19 open science practices. This core set of open science practices will form the foundation for institutional dashboards and may also be of value for the development of policy, education, and interventions.

PurposePoor maintenance management leads to non-negligible economic, environmental and social impacts and obstacles to the sustainable manufacturing paradigm. Studies evaluating maintenance impacts on sustainability underline growing interest in the topic, but reports on the industrial field are lacking. Therefore, this paper investigates the industrial environment and the indicators that manufacturing companies use for measuring their maintenance impacts.Design/methodology/approachIn this pilot survey study, several stakeholders of production enterprises in the south of Italy were interviewed to unveil the spread of the measurement of maintenance impacts on sustainability and the indicators used by those companies.FindingsThe interview results showed a low level of awareness among stakeholders about maintenance impacts on sustainability. Maintenance stakeholders are mainly focused on technical and economic factors, whereas environmental, quality and safety stakeholders are becoming more aware of maintenance impacts on environmental and social factors. However, both groups need guidelines to define sustainability indicators to assess such impacts.Originality/valueThis exploratory study allowed us to investigate the current situation in industrial organisations and achieve the first variegated and diversified vision of the awareness of company stakeholders on maintenance impacts on the sustainability of several business functions. This paper provides a valuable contribution to “maintenance and sustainability” research area in production contexts and sheds light on non-negligible maintenance impacts on sustainability, providing preliminary insights on the topic and an effective basis for defining future research opportunities. Moreover, this study enables increased awareness among internal and external manufacturing company stakeholders on the role of maintenance in sustainable production.

A Digital Twin is a virtual representation of a physical product, asset, process, system, or service that allows us to understand, predict, and optimise their performance for better business outcomes. Recently, the use of Digital Twin in industrial operations has attracted the attention of many scholars and industrial sectors. Despite this, there is still a need to identify its value in industrial operations mainly in production, predictive maintenance, and after‐sales services. Similarly, the implementation of a Digital Twin still faces many challenges. In response, a systematic literature review and analysis of 41 papers published between 2016 and 11 July 2020 have been carried out to examine recently published works in the field. Future research directions in the area are also highlighted. The result reveals that, regardless of the challenges, the role of Digital Twin in the advancement of industrial operations, especially production and predictive maintenance is highly significant. However, its role in after‐sales services remains limited. Insights are offered for research scholars, companies, and practitioners to understand the current state‐of‐the‐art and challenges, and to indicate future research possibilities in the field.

Background The brain is continuously targeted by a wealth of stimuli with complex spatio-temporal patterns and has presumably evolved in order to cope with those inputs in an optimal way. Previous studies investigating the response capabilities of either single neurons or intact sensory systems to external stimulation demonstrated that stimuli temporal distribution is an important, if often overlooked, parameter. Results In this study we investigated how cortical networks plated over micro-electrode arrays respond to different stimulation sequences in which inter-pulse intervals followed a 1/ f β distribution, for different values of β ranging from 0 to ∞. Cross-correlation analysis revealed that network activity preferentially synchronizes with external input sequences featuring β closer to 1 and, in any case, never for regular (i.e. fixed-frequency) stimulation sequences. We then tested the interplay between different average stimulation frequencies (based on the intrinsic firing/bursting frequency of the network) for two selected values of β , i.e. 1 (scale free) and ∞ (regular). In general, we observed no preference for stimulation frequencies matching the endogenous rhythms of the network. Moreover, we found that in case of regular stimulation the capability of the network to follow the stimulation sequence was negatively correlated to the absolute stimulation frequency, whereas using scale-free stimulation cross-correlation between input and output sequences was independent from average input frequency. Conclusions Our results point out that the preference for a scale-free distribution of the stimuli is observed also at network level and should be taken into account in designing more efficient protocols for neuromodulation purposes.