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Since children with chronic diseases represent a primary target for immunization strategies, it is important that their immunization coverage and timeliness of vaccines is optimal. We performed a study to measure immunization coverage and timeliness of vaccines in children with type 1 diabetes, HIV infection, Down syndrome, cystic fibrosis, and neurological diseases. A total of 275 children aged 6 months–18 years were included in the study. Coverage for diphtheria–tetanus–pertussis (DTP), polio (Pol), and hepatitis B (HBV) vaccines approximated 85% at 24 months, while measles–mumps–rubella (MMR) coverage was 62%. Immunization coverage for seasonal influenza was 59%. The analysis of timeliness revealed that there was heterogeneity among children with different chronic diseases. A proportional hazard model showed that children with HIV infection had the longest time to complete three doses of DTP, Pol, and HBV, and those with neurological diseases received the first dose of MMR later than the other categories. Causes of missing or delayed vaccination mostly included a concurrent acute disease. Children with chronic diseases should be strictly monitored for routine and recommended vaccinations, and health care providers and families should be properly informed to avoid false contraindications.

Although freshwater molluscs in the order Unionoida are considered one of the most endangered groups of animals in the world, sufficient data on their status are lacking for most species. As a result, a species may become rare, endangered, and even extinct before the first population assessment is conducted. This is especially true for endemic species, particularly those limited to remote regions with difficult access. We studied the current distribution and population densities of Popenaias popeii endemic to the Rio Grande drainage in Texas, and developed a method to evaluate changes in the population’s size and distributional range over the last 100 years. Sampling over 250 sites in four rivers that constitute the entire historical range of P. popeii in Texas, we found that this species has likely been extirpated from two rivers. The total length of the rivers populated by this mussel has declined by 75%, and the total P. popeii population size has declined by 72%. The remaining population of this species in the Rio Grande is fragmented, with only one 190-km stretch still supporting high densities. The developed approach could be used for other rare freshwater molluscs to reconstruct their historical range and population size.

Issue/Problem The risk of SARS-Cov-2 infection and its adverse health effects proved to be higher among socially disadvantaged groups, including migants and ethnic minorities. Hard-to-reach (HTR) migrants, such as undocumented people, those living in informal settelments (e.g squats) or roma people have experienced severe barries to access COVID-19 information, testing and vaccination services. Description of the problem During 2020 and 2021, the Migrants'Health Unit of Roma 2 Local Health Unit (ASL) developed different strategies to control the COVID-19 epidemics in HTR communities, addressing both the containment of clusters in informal settelments and access to COVID-19 vaccination for these population. Methods A multicomponent and multidisciplinary strategy was implemented, based on a strong collaboration of different services across the ASL and with Non Governmental Organizations (NGOs). Starting from a mapping of the settlements and the identification of the main critical issues for the control of the epidemic in the target populations, interventions were carried out that included the involvement of NGOS in active surveillance, reporting of suspected cases of COVID-19 to the ASL and information to the communities, and the reorganization of health interventions (eg, swabs, epidemiological investigations, COVID-19 vaccinations) directly in HTR communities’ life places. Results In the period from April 2020 to February 2021, 15 outbreaks were controlled, for a total of over 4500 persons reached, and 265 COVID-19 cases identified. From July to November 2021, vaccinations were offered in outreach or with dedicated vaccination sessions, which reached 1664 people. The intervention model, based on a deep context analysis, strong multisectoral collaboration, community involvement, lays the foundations for the design of public health strategies, not only aimed at HTR populations. Key messages • Controlling COVID-19 in Hard- to- reach migrant populations was possible thanks to a strong collaboration between public health services and NGOs. • Public health interventions addressed complex groups should envisage intersectoral collaborations, reorientation of services in order to meet target groups’ need and community involvement.

Background, aim and scope Street sediment samples were collected at 50 locations in a mixed land use area of Hamilton, Ontario, Canada, and metal levels were analyzed using a sequential extraction procedure for different particle size classes to provide an estimate of potential toxicity as well as the potential for treatment through best management practices (BMPs). Methodology The street sediment samples were dry sieved into four different particle size categories and a sequential extraction procedure was done on each size category following the methodology proposed by Tessier et al. 1979 using a Hitachi 180-80 Polarized Zeeman Atomic Absorption Spectrophotometer. Results and discussion Analysis of variance, post hoc least-significant difference tests, and kriging analysis showed that spatially Mn and Fe levels were associated with a well-defined heavy industrial area that includes large iron- and steel-making operations; Cu and Pb were associated with both the industrial and high-volume traffic areas, while Zn tended to be more associated with high-volume traffic areas. The potential bioavailability of the metals, based on the sum of chemical fractions 1 (exchangeable) and 2 (carbonate-bound), decreased in order: Zn > Cd > Mn > Pb > Cu > Fe. Based on aquatic sediment quality guidelines, there is some concern regarding the potential impact of the street sediment when runoff reaches receiving waters. Conclusions It is possible that a combination of BMPs, including street sweeping and constructed wetlands, could help to reduce street sediment impact on environmental quality in the Hamilton region. The data presented here would be important in developing and optimizing the design of these BMPs.

This communication describes the ongoing efforts towards the assessment of microwave imaging as a tool for real-time monitoring of thermal ablation treatments. In particular, the ex-vivo experimental set-up adopted for the validation is described, and the results of a preliminary experiment are shown. Notably, by analyzing pre- and post-ablation treatment data it is possible to recognize the footprint of the interface between the ablated and not-ablated tissue, making it possible to estimate the boundary of the treated area.

Postconditioning (PostC) can be obtained either with brief cycles of ischemia/reperfusion (I-PostC) or with a direct targeting of mitochondria with Diazoxide (pharmacological PostC, P-PostC). I-PostC may induce the activation of RISK and SAFE pathways and may favor nitric oxide production with S-Nitrosylation of proteins and redox signaling. It is not clear whether Diazoxide can lead to similar effects. We compared the effects of I-PostC and P-PostC on (a) kinases of RISK- and SAFE pathway, (b) S-Nitrosylation of mitochondrial proteins and (c) reduction of death signals (PKCδ, cleaved caspase-3 and Beclin-1) in cytosolic and mitochondrial fractions. Isolated rat hearts underwent (1) perfusion without ischemia (Sham), (2) ischemia/reperfusion (30-min ischemia plus 2-h reperfusion), (3) I-PostC (5 intermittent cycles of 10-s reperfusion and 10-s ischemia immediately after the 30-min ischemia), (4) P-PostC (Diazoxide 30 μM in the first of 3-min of reperfusion) or (5) I-PostC + MPG or P-PostC + MPG (MPG, 2-mercaptopropionylglycine 300 μM). Using Western blot and biotin switch assay, we found that P-PostC induced a redox sensible phosphorylation/translocation of Akt, ERK1/2 and GSK3β into the mitochondria, but not of phospho-STAT3, which was translocated into the mitochondria by I-PostC only. Either I-PostC or P-PostC increased mitochondrial S-Nitrosylated proteins (e.g., VDAC) and reduced the levels of phospho-PKCδ, cleaved caspase-3 and Beclin-1. Therefore, direct targeting of mitochondria with Diazoxide (a) activates the RISK pathway via a redox signaling, (b) favors discrete mitochondrial protein S-Nitrosylation, including VDAC and (c) decreases signals of death. Intriguingly, phospho-STAT3 translocation is induced by I-PostC, but not by P-PostC, thus suggesting a redox-independent mechanism in the SAFE pathway.

Wind energy is rapidly expanding its capacity as part of the global energy transition. To ensure economic viability, wind energy projects increasingly rely on risk mitigation strategies. While Power Purchase Agreements (PPAs) manage spot market price variability, wind farms still face energy production uncertainty, directly impacting the quantity of energy generated. This study presents a comprehensive analysis of the price markup for onshore and offshore wind farms, considering energy production uncertainty under PPA and non-PPA scenarios. The study examines key metrics such as equilibrium prices, price markups, Internal Rate of Return (IRR), Net Present Value (NPV), and their correlation to energy production risk. Results demonstrate that PPAs significantly alter the relationship between price markup and variability. Offshore wind farms can potentially benefit more from PPAs compared to onshore, especially at lower levels of wind energy variability. However, as variability increases, the risk mitigation provided by PPAs diminishes, and both onshore and offshore wind farms may require higher price markups for financial viability. These findings highlight the necessity of carefully designed PPA structures and pricing strategies to ensure long-term competitiveness and sustainability of wind energy projects.

We hypothesized that nandrolone (ND)-abuse induces cardiac hypertrophy, increases myocardial susceptibility to ischemia/reperfusion (I/R) injury, and reduces responsiveness to postconditioning (PostC) cardioprotection. Wistar-rats were ND treated for 2 weeks (short_ND) or 10 weeks (long_ND). Vehicle-treated rats served as controls. Hearts were retrogradely perfused and left ventricular pressure (LVP) was measured before and after 30-min global ischemia. In subgroups of hearts, to induce cardioprotection a PostC protocol (five cycles of 10-s reperfusion and 10-s ischemia) was performed. β-adrenoreceptors, kinases (Akt and GSK-3β) and phosphatases (PP2A sub A and PP2A sub B) were examined by Western blot before and after ischemia. After 120-min reperfusion, infarct size was measured. Short_ND slightly increased cardiac/body weight ratio, but did not affect cardiac baseline nor post-ischemic contractile function or infarct size when compared to vehicle hearts. However, PostC limited cardiac dysfunction much more in short_ND hearts than the other groups. Although cardiac/body weight ratio markedly increased after long_ND, baseline LVP was not affected. Yet, post-ischemic contracture and infarct size were exacerbated and PostC was unable to reduce infarct size and ventricular dysfunction. While short_ND increased phosphatases, non-phosphorylated and phosphorylated Akt, long_ND reduced phosphatase-expression and Akt phosphorylation. Both short_ND and long_ND had no effect on the GSK-3β-phosphorylation but increased the expression of β 2 -adrenoreceptors. In reperfusion, PostC increased Akt phosphorylation regardless of protective effects, but reduced phosphatase-expression in protected hearts only. In conclusion, short_ND improves post-ischemic myocardial performance in postconditioned hearts. However, long_ND increases myocardial susceptibility to I/R injury and abolishes cardioprotection by PostC. This increased susceptibility might be related to steroid-induced hypertrophy and/or to altered enzyme expression/phosphorylation.

Background/aim:Endotoxaemia can complicate hepatic ischaemia–reperfusion (IR) injury. Endocannabinoids appear to modulate the haemodynamic alterations and cytokine response induced by lipopolysaccharide (LPS). Thus, we aimed to determine the effect of the endocannabinoid CB1-receptor antagonist Rimonabant in a model of hepatic IR injury complicated by endotoxaemia.Methods:Sprague–Dawley rats pre-treated with Rimonabant 3 or 10 mg/kg or vehicle underwent partial hepatic IR and lipopolysaccharide (LPS) injection at reperfusion. Liver injury was evaluated by serum alanine aminotransferase (ALT) and necrotic-cell count. The inflammatory response was investigated by assessing hepatic neutrophil infiltration, tumour necrosis factor α (TNFα), interferon γ (IFNγ), interleukin 6 (IL6), and suppressor of cytokine signalling (SOCS) 1 and SOCS3 gene expression by real-time polymerase chain reaction (RT-PCR). Systolic blood pressure and hepatic blood flow were measured as haemodynamic parameters. Finally, lipid peroxidation, glutathione status, and immunoreactive CB1 receptor expression in the liver were also determined.Results:Liver injury and neutrophil infiltration occurring in the late-phase of LPS-enhanced IR were significantly reduced by CB1-receptor antagonism. Rimonabant-treated rats showed significantly higher gene expression of IFNγ, IL6, SOCS1 and SOCS3 in “early” reperfusion, while that of TNFα was reduced. These findings were associated with increased STAT3 phosphorylation. Furthermore, CB1-receptor antagonism significantly improved the oxidative injury and haemodynamic alterations occurring during reperfusion in untreated rats. Finally, CB1-receptor immunoreactivity was upregulated early after reperfusion.Conclusions:This study demonstrates that CB1-receptor antagonism protects the liver against LPS-enhanced IR injury by interfering with the inflammatory response that causes the late, neutrophil-dependent phase of reperfusion injury, although the prevention of the transient endotoxin-related hypotension occurring early during reperfusion may be also involved.