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Controlling the motional degrees of isolated, single nanoparticles trapped within optical fields in a high vacuum are seen as ideal candidates for exploring the limits of quantum mechanics in a new mass regime. These systems are also massive enough to be considered for future laboratory tests of the quantum nature of gravity. Recently, the translational motion of trapped particles has been cooled to microkelvin temperatures, but controlling all the observable degrees of freedom, including their orientational motion, remains an important goal. Here we report the control and cooling of all the translational and rotational degrees of freedom of a nanoparticle trapped in an optical tweezer, accomplished by cavity cooling via coherent elliptic scattering. We reached temperatures in the range of hundreds of microkelvins for the translational modes and temperatures as low as 5 mK for the librational degrees of freedom. This work brings within reach applications in quantum science and the study of single isolated nanoparticles via imaging and diffractive methods, free of interference from a substrate.Optically trapped and levitated nanoparticles can be used to study macroscopic quantum effects, but fully controlling their motion is difficult. Now, all six roto-translational degrees of freedom have been cooled, although not to the quantum ground state.

In recent years, levitated optomechanics has delivered on the promise of reaching the motional quantum ground state. An important next milestone of the field would be the generation of mechanical entanglement. An ideal candidate is the two-dimensional motion in the polarization plane of an optical tweezer inside an optical cavity, where optical and mechanical modes are coupled via coherent scattering. Achieving this goal requires two key conditions: two-dimensional ground state cooling along with substantial spectral overlap between the two modes. The latter is essential to generate the necessary correlations, but unfortunately, it hinders efficient cooling thus narrowing the useful parameter space. In this work, we report the achievement of a high purity two-dimensional state in a regime where the strong optomechanical coupling induces the desired spectral overlap between oscillations in different directions, as reflected in the non-trivial spectral shape of the detected cavity field. As a result, significant correlations consistently arise between any pair of orthogonal directions, preventing the motion from being reduced to two independent one-dimensional oscillators and leading to higher purity compared to that scenario. Our system serves as an excellent platform for realizing continuous variable entanglement in two-dimensional motion. By optically cooling the motion of a levitated nanosphere, a high purity two-dimensional state is achieved in a regime of strong optomechanical coupling. A significant quantum mutual information arises between pairs of orthogonal directions.

We study the role of stimulated Brillouin scattering in a fiber cavity by numerical simulations and a simple theoretical model and find good agreement between experiment, simulation and theory. We also investigate an optomechanical system based on a fiber cavity in the presence of the nonlinear Brillouin scattering. Using simulation and theory, we show that this hybrid optomechanical system increases optomechanical damping for low mechanical resonance frequencies in the unresolved sideband regime. Furthermore, optimal damping occurs for blue detuning in stark contrast to standard optomechanics. We investigate whether this hybrid optomechanical system is capable of cooling a mechanical oscillator to the quantum ground state.

In recent years, quantum phenomena have been experimentally demonstrated on variety of optomechanical systems ranging from micro-oscillators to photonic crystals. Since single photon couplings are quite small, most experimental approaches rely on the realization of high finesse Fabry-Perot cavities in order to enhance the effective coupling. Here we show that by exploiting a, long path, low finesse fiber Fabry-Perot interferometer ground state cooling can be achieved. We model a 100 m long cavity with a finesse of 10 and analyze the impact of additional noise sources arising from the fiber. As a mechanical oscillator we consider a levitated microdisk but the same approach could be applied to other optomechanical systems.

Background Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology. However, there is no information on the transcriptome response triggered by UV-B in grapevine. In this paper we investigate the gene expression responses of leaves from in vitro cultured Vitis vinifera cv. Malbec plants subjected to the same dose of biologically effective UV-B radiation (4.75 kJ m -2 d -1 ) administered at two different fluence rates (16 h at ≅ 8.25 μW cm -2 , 4 h at ≅ 33 μW cm -2 ) using a new custom made GrapeGen Affymetrix GeneChip ® . Results The number of genes modulated by high fluence rate UV-B doubled the number of genes modulated by low fluence UV-B. Their functional analyses revealed several functional categories commonly regulated by both UV-B treatments as well as categories more specifically modulated depending on UV-B fluence rate. General protective responses, namely the induction of pathways regulating synthesis of UV-B absorbing compounds such as the Phenylpropanoid pathway, the induction of different antioxidant defense systems and the activation of pathways commonly associated with pathogen defense and abiotic stress responses seem to play critical roles in grapevine responses against UV-B radiation. Furthermore, high fluence rate UV-B seemed to specifically modulate additional pathways and processes in order to protect grapevine plantlets against UV-B-induced oxidative stress, stop the cell cycle progression, and control protein degradation. On the other hand, low fluence rate UV-B regulated the expression of specific responses in the metabolism of auxin and abscisic acid as well as in the modification of cell walls that could be involved in UV-B acclimation-like processes. Conclusion Our results show the UV-B radiation effects on the leaf transcriptome of grapevine ( Vitis vinifera cv. Malbec) plantlets. Functional categories commonly modulated under both UV-B treatments as well as transcripts specifically regulated in an UV-B-intensity dependent way were identified. While high fluence rate UV-B had regulatory effects mainly on defense or general multiple-stress responses pathways, low fluence rate UV-B promoted the expression of genes that could be involved in UV-B protection or the amelioration of the UV-B-induced damage. This study also provides an extensive list of genes regulating multiple metabolic pathways involved in the response of grapevine to UV-B that can be used for future researches.

Posterior tibial tendon (PTT) dysfunction is three times more common in females, and some patients may have a predisposition without a clinically evident cause, suggesting that individual characteristics play an important role in tendinopathy. The present study investigated the association of rs4986938 (+ 1730G > A; AluI RFLP) and rs1256049 (− 1082G > A; RsaI RFLP) single nucleotide polymorphisms (SNPs) of estrogen receptor-beta (ER-β) gene with PTT dysfunction. A total of 400 participants were recruited. The PTT dysfunction group: these patients underwent surgery, with PTT tendinopathy confirmed by histopathology and magnetic resonance image (MRI). The control group was composed of participants with no clinical or MRI evidence of PTT dysfunction. Each group was composed of 100 postmenopausal women, 50 premenopausal women, and 50 men. Genomic DNA was extracted from saliva samples, and genotypes were obtained by polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP). Concerning the ER-β SNP rs4986938, there were significant differences in the frequencies of alleles between test and control groups of all the cases, only postmenopausal women and only men (p < 0.0001, p = 0.0016 and p = 0.0001). Considering the PTT dysfunction group and comparing postmenopausal women versus premenopausal women adding men, the analysis showed significant differences in the allelic distribution (p = 0.0450): the allele A in postmenopausal women is a risk factor. The ER-β SNP rs1256049 did not show differences in the frequencies of alleles and genotypes between groups. The ER-β SNP rs4986938, but not ER -β SNPs rs1256049, may contribute to PTT insufficiency in the Brazilian population, with additional risk in postmenopausal women. Addition, in men the genetic factor could be more determinant.

Category: Midfoot/Forefoot Introduction/Purpose: The Lapidus procedure (LP) is a powerful technique that often provides significant corrections of the hallux valgus (HV) deformities. In addition, it is described that forefoot width alters considerably with this technique, impacting in shoe wear. However, it has not yet been reported whether foot narrowing reflects on clinical and functional outcomes. This study aimed to evaluate the effect of variations in bony and soft tissue foot widths (BSFW) on clinical and functional outcomes after HV correction with the LP. Secondarily, we also tried to assess the relationship of standard radiographic measurements of HV with changes in BSFW. Methods: Forty-three feet in 35 patients with a mean follow-up of 18.5-months who underwent the LP were retrospectively reviewed. Bony width was measured from the most medial extent of the first metatarsal head to the most lateral extent of the fifth metatarsal head. Soft tissue width was measured considering the most medial and lateral shadow of the foot (Figure 1). Clinical and functional data were assessed with the VAS for pain, AOFAS, LEFS and SF-12. SF-12 comprises physical and mental health scales (PCS-12 and MCS-12). HV radiographic parameters as intermetatarsal-angle (IMA), HV-angle (HVA) and sesamoid- subluxation (SS) were assessed. Intraclass Correlation Coefficients (ICC) were calculated for BSFW. Clinical, functional and radiographic measurements were compared. Linear regression was used to assess the correlation of Δ-BSFW with clinical, functional and radiographic results. We also evaluated our sample at the cut-off point of quartile-50% to assess whether a given measure of Δ-BSFW was related to clinical and functional outcomes. Results: Pre- and postoperative ICC was 0.95 for bony and soft tissue width measurements. Bony width changed significantly from 95.5mm to 84.2mm (11.8%) and soft tissue width from 107.12mm to 100.84mm (5.86%) (p<.001 and p<.001). IMA, HVA and SS improved significantly. Significant clinical and functional improvements were observed, except in MCS-12. In simple linear regression, a correlation was found between variations of bony width with Δ-AOFAS and Δ-PCS-12, meaning that as the forefoot narrows, their values increase (p=.02 and p=.005). It was also related to Δ-IMA and Δ-SS, meaning that the forefoot narrows as these parameters improve (p<001 and p<0.001). Soft tissue width was related only to Δ-PCS-12 and Δ-IMA. In multiple linear regression, the strongest correlation observed was between bony width variation and Δ-IMA (P=.029,r2=0.22). At the quartile- 50%, in which our sample was divided equally, we have not found a given measure of Δ-BSFW that was significantly related to the clinical and functional questionnaires (Figure 2). Conclusion: The present study showed that narrowing of the forefoot after the LP improves clinical and functional outcomes, as measured by AOFAS and PCS-12. Besides, it also presented that correction of the radiographic parameters of the HV, mainly IMA, reflects on a significant decrease of the forefoot width. Further prospective and comparative studies with larger populations are required to evaluate the effects of forefoot width changes on clinical and functional outcomes.

Background Posterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic single-nucleotide polymorphism (SNP) and tendinopathy. In the present study, we investigated the association of PvuII c454-397T>C (NCBI ID: rs2234693) and XbaI c454-351A>G (NCBI ID: rs9340799) SNPs in estrogen receptor alfa (ER-α) gene with PPT dysfunction. Methods A total of 92 female subjects with PTT dysfunction, with histopathological examination of the tendon and magnetic resonance image (MRI) evidence of tendinopathy, were compared to 92 asymptomatic females who presented an intact PPT at MRI for PvuII and XbaI SNPs in the ER-α gene. Genomic DNA was extracted from saliva and genotypes were obtained by polymerase chain reaction restriction fragment length polymorphism. Results The analysis of PvuII SNPs showed no significant differences in the frequency of alleles and genotypes between control and PTT dysfunction groups. The XbaI SNPs in the ER-α gene showed significant differences in the frequency of genotypes between control and test groups ( p  = 0.01; OR 95% 1.14 (0.55–2.33). Conclusions The XbaI SNP in the ERα gene may contribute to tendinopathy, and the A/A genotype could be a risk factor for PTT tendinopathy in this population. The PvuII SNP studied was not associated with PTT tendinopathy.

Posterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic single-nucleotide polymorphism (SNP) and tendinopathy. In the present study, we investigated the association of PvuII c454-397T>C (NCBI ID: rs2234693) and XbaI c454-351A>G (NCBI ID: rs9340799) SNPs in estrogen receptor alfa (ER-[alpha]) gene with PPT dysfunction. A total of 92 female subjects with PTT dysfunction, with histopathological examination of the tendon and magnetic resonance image (MRI) evidence of tendinopathy, were compared to 92 asymptomatic females who presented an intact PPT at MRI for PvuII and XbaI SNPs in the ER-[alpha] gene. Genomic DNA was extracted from saliva and genotypes were obtained by polymerase chain reaction restriction fragment length polymorphism. The analysis of PvuII SNPs showed no significant differences in the frequency of alleles and genotypes between control and PTT dysfunction groups. The XbaI SNPs in the ER-[alpha] gene showed significant differences in the frequency of genotypes between control and test groups (p = 0.01; OR 95% 1.14 (0.55-2.33). The XbaI SNP in the ER[alpha] gene may contribute to tendinopathy, and the A/A genotype could be a risk factor for PTT tendinopathy in this population. The PvuII SNP studied was not associated with PTT tendinopathy.

Category: Basic Sciences/Biologics Introduction/Purpose: Numerous studies, as evident in literature, have attempted to develop an induced Achilles tendinopathy animal model. The most common models include collagenase injections and mechanical overload of the tendon, but both are controversial. The mechanical overload model creates a chronically induced tendinopathy and is time intensive. In addition, once the stimulus is removed the tissue changes often heal. The collagenase injection model is quicker, rarely evolve to healing but frequently results in an early acute tendon reaction. The primary objective of this study is to compare biomechanical and histological findings between two collagenase induced Achilles tendinopathy protocols. The hypothesis is that consecutive low-dose collagenase injections will result in progressive and long-lasting tendinopathy findings as compared to the traditional single high-dose injection. Methods: In this IRB approved study, the population was composed of forty-eight (n=48) New Zealand breed rabbits. Forty-two (n=42) rabbits were randomly divided into two groups (n=21). The first group, which served as the control, had both Achilles tendons injected by a single dose (0.3 mg) of A1 collagenase (Sigma-Aldrich©), as described in literature. The second experimental group had three low-dose injections (0.10 mg) with 2 weeks between each injection. Another six animals (n=6) were also randomized into two groups (n=3) receiving one-dose versus 3 injections of saline solution with 2 weeks between each injection. The animals were euthanized after 10, 12 and 16 weeks. Histological and biomechanic analysis of the Achilles tendons were carried out using a dynamic mechanical testing machine (Electropuls®, model E10000, Instron®). Mechanical strength and histological scores of tendinopathy (Bonar scoring system) were compared among the groups at each time-point. Results: After 16 weeks, all biomechanical and histological parameters analysed showed consistent differences between the groups (p < 0.05), with more pronounced and long-lasting tendinopathy findings in the Achilles tendon of the experimental group (serial collagenase injections), when compared to the control groups (single collagenase dose and single/multiple saline injections). The mean Bonar sum-score of tendons after 16 weeks in the experimental group was greater than the mean histologic score of control tendons (8.53 ± 1.52 versus 1.1 ± 0.83 in saline group and 5.2 ± 1.12 in single dose group). No statistically significant differences were found between the different collagenase injection groups at weeks 10 and 12. Conclusion: The protocol of three consecutive low-dose collagenase injections has shown biomechanical and histological findings compatible with progressive and long-lasting tendinopathy as compared to single high-dose injection. This protocol represents a feasible and effective animal model of induced Achilles tendinopathy.