Explore the vast range of titles available.

The glycosaminoglycan chondroitin sulfate is a critical component of proteoglycans on the cell surface and in the extracellular matrix. As such, chondroitin sulfate side chains and the sulfation balance of chondroitin play important roles in the control of signaling pathways, and have a functional importance in human disease. In contrast, very little is known about the roles of chondroitin sulfate molecules and sulfation patterns during mammalian development and cell lineage specification. Here, we report a novel biphasic role of chondroitin sulfate in the specification of the cardiac cell lineage during embryonic stem cell differentiation through modulation of Wnt/beta-catenin signaling. Lineage marker analysis demonstrates that enzymatic elimination of endogenous chondroitin sulfates leads to defects specifically in cardiac differentiation. This is accompanied by a reduction in the number of beating cardiac foci. Mechanistically, we show that endogenous chondroitin sulfate controls cardiac differentiation in a temporal biphasic manner through inhibition of the Wnt/beta-catenin pathway, a known regulatory pathway for the cardiac lineage. Treatment with a specific exogenous chondroitin sulfate, CS-E, could mimic these biphasic effects on cardiac differentiation and Wnt/beta-catenin signaling. These results establish chondroitin sulfate and its sulfation balance as important regulators of cardiac cell lineage decisions through control of the Wnt/beta-catenin pathway. Our work suggests that targeting the chondroitin biosynthesis and sulfation machinery is a novel promising avenue in regenerative strategies after heart injury.

The article reconciles contentious issues between code biology and biosemiotics. The framework of semantic biology and molecular meaning woven around organic codes by Marcello Barbieri defied classical informational conceptualizations of meaning in the biological realm while the discourse on meaning and agency and their respective (in)dependency on interpretation continues. Whereas the role of codes in agency is often secondary to other aspects, I present here a more consistent picture– integrating codes and artifacts to smoothly transcend from the world of mechanistic molecular meaning to organismic agency. Interpretation is presented as a call-to-action based on intraindividual states, and sense-making as call-to-change based on interindividual communication.

The use of sustainably sourced biomass is an important tool for mitigating the effects of climate change; but biomass is far from being a homogeneous resource. The aim of this study was to examine the decision-making process of industrial end-users considering biomass procurement. An online, two-part survey generated responses from 27 experienced professionals, representing a portfolio of facilities varying in size, technology, and biomass types, across Australia, Canada, Finland, and Sweden. A PAPRIKA conjoint analysis approach was used to analyze the data so that the attributes that influenced procurement decisions could be weighted and ranked. The results provided an insight into end-users’ views on factors including facility location, size, and biomass storage, handling, and procurement for different wood-based industrial services. The most important decision-making attribute appeared to be the type of biomass assortment, at individual, national, and aggregated levels. Of seven sub-categories of biomass assortments, sawdust (35%) was the most preferred type followed by stem wood chips (20%) and energy wood (15%). We concluded that, from the end-user’s perspective, a pre-defined biomass assortment is the most important factor when deciding on feedstock procurement at a bioenergy facility. These results help us better understand end-users’ perceptions of biomass properties in relation to their conversion processes and supply preferences and can inform product development and the securement of new niches in alternative business environments by existing and future biohubs.

There is currently great general interest in reducing the use of fossil-based materials. Fossil-based tarps are still widely used as cover for wood chip storage piles, causing additional waste or requiring further waste treatment in the supply chain. This study aimed to investigate the performance of an innovative bio-based wood chip pile cover compared to conventional treatments (plastic-covered and uncovered) in eastern Finnish conditions. The experiment evaluated the drying process during the storage of stemwood chips during 5.9 months of storage. It included the developments of temperature, moisture content, heating value, energy content, basic density, particle size distribution, and the dry matter losses of a total of six piles. As a result, the forest stemwood chips dried by 11%, with dry-matter losses of 4.3%, when covered with the bio-pile cover. Using the plastic covering, the forest stemwood chips dried by 22%, with dry matter losses of 2.9%. At the end of the experiment, the energy content in plastic-covered piles was 6.1% higher than uncovered piles and 3.1% higher than bio-pile-covered piles. While differences in the key drying performance parameters can be observed, the differences between uncovered piles and those covered with plastic tarps, as well as between the bio-based and the uncovered piles, were not statistically significant. We conclude that the bio-based cover, under the studied conditions, do not render better storage conditions than in current practices. However, our study indicates possible fossil-substitutional benefits by using a bio-based cover, which calls for further R&D work in this matter.

The visible human body is composed of flesh and bones for the most part, yet an invisible orchestra of sensations and perceptions creates a virtual or phantom body that behaves like a shadow following every movement and gesture of its anatomical complement. This shadow becomes only “visible” to the individual when bodily integrity is affected, anatomically or cognitively. Phantom limbs have been known for a long time. They refer to the felt presence of a missing hand, leg, or other body part as if it was still in place. Reciprocally and of a supposedly cognitive origin, phantom extremities are reported by some patients that feel the virtual presence of a supernumerary limb – signifying anatomical “overcompleteness.” However, other patients feel as one of their limbs does not belong to their body – signifying “foreignness”. Various shades of the so-called body integrity identity disorder exemplify the assumed complex signification processes within the human body. The Peircean theory of signs and the Uexküllian concept of endosemiosis are combined to approach the still poorly understood phantom phenomena in light of representation and embodiment.

The comminution of fuelwood for efficient transportation and handling exposes the material to various biological and chemical decomposition processes. The stockpiling of fuel chips can result in significant dry matter losses (DML) and consequent release of CO2 into the atmosphere. The decomposition processes could be controlled by managing the chip moisture content (MC). MC control by utilizing the self-heating of stockpiled stemwood chips together with wind-driven ventilation was tested in a practical storage experiment, using uncovered and plastic-covered piles as references. The data were analyzed with linear mixed models. The predicted DML was 2.4–3.8% during the monitoring period of 5.9 months, but no significant differences appeared between the storage treatments. The increase in the basic density of the chips decreased DML. On average 1.7–3.5% of the recoverable energy content of the chips was lost during the experiment. The predicted average decline in the MC was ca. 4–8 percentage points (p.p.). The MC of the chip samples stored under plastic tarp was 4–5 p.p. lower than those stored in the uncovered piles. Heat generation within the piles was modest due to the high quality of the chips, and the ventilation solution tested only marginally affected the drying process and the mitigation of DML.

The objective of this study was to determine the economic performance of alternative chipper choices for small-scale chipping based on unit cost (€ per chip-m3) and net present value (NPV) calculations. For the chipping cost and investment profitability analyses four tractor-powered professional or semi-professional disc chippers and two professional drum chippers mounted on a truck or powered by tractor were selected. Initial investment, operating costs, and the cost of outsourced chipping were the key elements for comparing the profitability of investment alternatives. The average purchase prices, cost factors, and technical details of the chipper units were acquired from machine dealers, specification sheets, a literature review, and interviews with chipping entrepreneurs. The results of the three tractor-powered professional chippers involved in the comparison were very close to each other. The profitable running of a truck-mounted drum chipper calls for high annual chipping volumes: the chipper type is therefore a feasible choice for an entrepreneur in large-scale chipping. Semi-professional disc chippers offer lower investment costs, but their economic feasibility is relatively poor.

The glycosaminoglycan chondroitin sulfate is a critical component of proteoglycans on the cell surface and in the extracellular matrix. As such, chondroitin sulfate side chains and the sulfation balance of chondroitin play important roles in the control of signaling pathways, and have a functional importance in human disease. In contrast, very little is known about the roles of chondroitin sulfate molecules and sulfation patterns during mammalian development and cell lineage specification. Here, we report a novel biphasic role of chondroitin sulfate in the specification of the cardiac cell lineage during embryonic stem cell differentiation through modulation of Wnt/beta-catenin signaling. Lineage marker analysis demonstrates that enzymatic elimination of endogenous chondroitin sulfates leads to defects specifically in cardiac differentiation. This is accompanied by a reduction in the number of beating cardiac foci. Mechanistically, we show that endogenous chondroitin sulfate controls cardiac differentiation in a temporal biphasic manner through inhibition of the Wnt/beta-catenin pathway, a known regulatory pathway for the cardiac lineage. Treatment with a specific exogenous chondroitin sulfate, CS-E, could mimic these biphasic effects on cardiac differentiation and Wnt/beta-catenin signaling. These results establish chondroitin sulfate and its sulfation balance as important regulators of cardiac cell lineage decisions through control of the Wnt/beta-catenin pathway. Our work suggests that targeting the chondroitin biosynthesis and sulfation machinery is a novel promising avenue in regenerative strategies after heart injury.

Amongst all the working elements of single grip harvesters, the working elements of felling and processing play an important role within the cut-to-length (CTL) harvester’s working phases. This includes the felling cutting and cross-cutting of stems within the felling and processing operation. The detailed investigation of such individual machine activities may help to analyse and improve the performance of forest machines. The objective of this study was to investigate the cutting duration and other performance parameters, including the fuel consumption, of a CTL harvester’s sawing unit under real working conditions. Detailed information on the felling cutting and cross-cutting performance was collected at short intervals using CAN bus data of two single grip harvesters in final felling from two different sites in Eastern Finland. As a result, models for effective time consumption in the work phase of cutting as a function of stem size were developed, both for felling cutting and cross-cutting. Felling cutting and cross-cutting durations were somewhat identical until the cutting diameters of 400–450 mm, depending on the site. Thereafter, the cutting time difference increased and was higher in felling cutting. At the site with large diameters of 550–650 mm, the difference varied between 15% and 28%, between the comparisons of formulae. In addition, other performance parameters, including the respective fuel consumption of this working phase, were part of the study. The study revealed a higher hourly based fuel consumption for the entire guide bar movement time compared to the pure cutting time in cross-cutting with stem diameters below 400 mm. Detailed knowledge of the performance of the sawing unit’s activities might help the planning process of future studies, as well as support the future development of efficient and intelligent machinery.

The fuel supply of forest chips has to adapt to the annual fluctuations of power and heat generation. This creates inefficiency and unbalances the capacity utilization of the fuel supply fleet in the direct fuel supplies from roadside storages to power and heat generation. Terminals can offer an alternative approach for the fleet management of fuel supplies in terms of smoothing the unbalanced fleet use towards more even year‐round operations. The aim of the study was to compare the supply costs of a conventional direct forest chip supply to an alternative fuel supply with the use of a feed‐in terminal using the discrete‐event simulation method. The influences of the terminal location, terminal investment cost, outbound terminal transport method, terminal truck utilization and quality changes of terminal‐stored forest chips for the fuel supply cost were studied in the case environment. By introducing a feed‐in terminal and a shuttle truck for the transports of terminal‐stored forest chips, the total supply cost was 1.4% higher than the direct fuel supply scenario. In terminal scenarios, the supply costs increased 1–2% if the cost of the terminal investment increased 30%, the distance to the terminal increased from 5 to 30 km or the total annual use of a terminal truck decreased 1500 h. Moreover, a 1 per cent point per month increase in the dry matter loss of terminal‐stored chips increased the total supply cost 1%. The study revealed that with the relatively low additional cost, the feed‐in terminal can be introduced to the conventional forest chip supply. Cost compensation can be gained through the higher annual use of a fuel supply fleet and more secured fuel supply to power plants by decreasing the need for supplement fuel, which can be more expensive at a time of the highest fuel demand. Discrete event simulation‐based system analysis model for supply chain logistics of forest chips. Terminal‐based forest chip supply may effectively balance work opportunities and reform the fuel supply towards more stable year‐round activity with relatively low additional costs compared to conventional direct fuel supply. While using terminal supply as a part of forest fuel deliveries, the studied simulation scenarios revealed the impacts of alternative fuel supply setups and quality changes of the fuel on the operation performances and supply costs. It was noted, for example, that a 30% increase in the terminal investment costs or 25 km longer distance to the terminal resulted in 1–2% increase in the supply costs.