Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
1,625
result(s) for
"Product "
Sort by:
Candida albicans-Epithelial Interactions: Dissecting the Roles of Active Penetration, Induced Endocytosis and Host Factors on the Infection Process
Candida albicans frequently causes superficial infections by invading and damaging epithelial cells, but may also cause systemic infections by penetrating through epithelial barriers. C. albicans is a remarkable pathogen because it can invade epithelial cells via two distinct mechanisms: induced endocytosis, analogous to facultative intracellular enteropathogenic bacteria, and active penetration, similar to plant pathogenic fungi. Here we investigated the contributions of the two invasion routes of C. albicans to epithelial invasion. Using selective cellular inhibition approaches and differential fluorescence microscopy, we demonstrate that induced endocytosis contributes considerably to the early time points of invasion, while active penetration represents the dominant epithelial invasion route. Although induced endocytosis depends mainly on Als3-E-cadherin interactions, we observed E-cadherin independent induced endocytosis. Finally, we provide evidence of a protective role for serum factors in oral infection: human serum strongly inhibited C. albicans adhesion to, invasion and damage of oral epithelial cells.
Journal Article
Successful cryopreservation of matured testis and ovary for the short barbeled velvetchin (Hapalogenys nitens)
As an important germplasm resource of fish, the cryopreservation of testis and ovary is of great significance to protect endangered species and increase genetic diversity. However, current methods of slow cooling and vitrification in gonad preservation require a specialized cooling equipment or a higher concentration of cryoprotectants to maintain cell viability. The short barbeled velvetchin ( Hapalogenys nitens ) is an important marine economic fish, and the germplasm resources have been degraded during long-term artificial breeding. Therefore, this study isolated the gonads of mature Hapalogenys nitens and investigated the cryopreservation effect of testis and ovary with three cryoprotectant combinations under four freezing procedures. The results showed that the gonad tissues were cut to blocks of 0.5 cm 3 , which could effectively cryopreserve the testes or ovaries with the cryoprotectant combinations of 15% ethylene glycol, 10% dimethyl sulfoxide, 0.2 M trehalose or 15% propylene glycol, 0.2 M trehalose, and 15% fetal bovine serum, respectively. The testes with cryoprotectants were only kept 5 cm above liquid nitrogen for 10 min and then immersed in liquid nitrogen, while the ovaries soaked in cryoprotectants were directly stored in the refrigerator at -80°C. After 7 days, the gonads were thawed in a water bath at 10°C for 8 min and analyzed by morphology, and the cell viability was measured by trypan blue or cell viability assay kits, resulting in a high survival rate (>90%). The present study successfully established cryopreservation protocols of gonad tissues in Hapalogenys niten . This was a convenient, rapid, and efficient method for the gonad cryopreservation of Sparidae fishes and provided reference for the preservation of other fish germplasm resources.
Journal Article
Click : how to make what people want : a proven system from the world's most successful startups
\"From the New York Times bestselling authors of Sprint, a guide for starting big projects the smart way-based on firsthand experience with more than three hundred new products and businesses\"-- Provided by publisher.
BOOK
An evolutionary game analysis of the promotion of shipborne low-temperature freezing technology for sea-caught shrimps among the government, fishermen, and consumers
In the era of heightened focus on sustainable marine fisheries development, the Shipborne Low-Temperature Freezing Technology is crucial for enhancing the preservation effect of seafood, improving transportation efficiency, and reducing energy consumption. It plays a key role in the sustainable development of the fishery industry. This paper constructs a tripartite evolutionary game model involving fishermen, consumers, and the government by using evolutionary game theory, and analyzes the interaction mechanism of the behaviors of the three parties. Based on field survey data, through model derivation and simulation, with results as follows: (1) Government subsidies positively influence choices at both the production and consumption ends. They incentivize fishermen to adopt Shipborne Low-Temperature Freezing Technology for storing wild-caught shrimp products, while also encouraging consumers to purchase shrimp products preserved through this technology. (2) Government subsidy structures have complex effects on the strategic choices of both fishermen and consumers. Expanding subsidies to fishermen positively impacts the adoption of Shipborne Low-Temperature Freezing Technology. (3) An appropriate online sales ratio can generate positive cross-channel effects, enabling the game system to reach an optimal equilibrium. In this state: Fishermen spontaneously adopt Shipborne Low-Temperature Freezing Technology; Consumers voluntarily purchase wild-caught shrimp preserved with Shipborne Low-Temperature Freezing Technology; The government withdraws subsidies for both fishermen and consumers.
Journal Article
Compost microbiomes in the plastisphere: microplastic fate and trade-offs in organic waste valorisation
Composting is widely promoted for organic waste valorisation, but a growing body of evidence shows that compost products often contain microplastics (MPs), raising concerns over secondary pollution. Within composting systems, plastisphere microbiomes colonize plastic particles, on which biofilms mediate the formation of distinct microenvironments and can modulate surface oxidation, partial depolymerisation, and fragmentation. Although biodegradable polymers may mineralise under thermophilic conditions, conventional plastics persist and may shift toward smaller fragments, potentially including nanoplastics, highlighting a sustainability paradox: composting mitigates waste and supplies nutrients, but simultaneously can contribute to MP dissemination. Once applied to soils, compost-derived MPs can disrupt soil aggregation, microbial balance, and plant–microbe interactions. Plants exposed to MPs exhibit oxidative stress responses—including stress-related responses and impaired plant performance under certain exposure scenarios—along with reduced photosynthetic efficiency and biomass. Soil fauna ingest MPs, leading to intestinal damage, oxidative imbalance, and trophic transfer to higher organisms. Human exposure via compost-amended agroecosystems is plausible, but exposure attribution and toxicological thresholds remain uncertain. Despite advances in microbial and enzymatic degradation, their efficiency under realistic composting conditions remains poorly constrained because plastisphere activity is highly context-dependent and difficult to capture in simplified laboratory settings. This mini-review synthesises current evidence on compost plastisphere ecology and polymer-dependent transformation constraints, and discusses methodological and field-scale limitations that shape interpretation, with emphasis on size-resolved risk framing, mixture contexts, and cross-kingdom interactions.
Journal Article
Utility of constraints reflecting system stability on analyses for biological models
Simulating complex biological models consisting of multiple ordinary differential equations can aid in the prediction of the pharmacological/biological responses; however, they are often hampered by the availability of reliable kinetic parameters. In the present study, we aimed to discover the properties of behaviors without determining an optimal combination of kinetic parameter values (parameter set). The key idea was to collect as many parameter sets as possible. Given that many systems are biologically stable and resilient (BSR), we focused on the dynamics around the steady state and formulated objective functions for BSR by partial linear approximation of the focused region. Using the objective functions and modified global cluster Newton method, we developed an algorithm for a thorough exploration of the allowable parameter space for biological systems (TEAPS). We first applied TEAPS to the NF-κB signaling model. This system shows a damped oscillation after stimulation and seems to fit the BSR constraint. By applying TEAPS, we found several directions in parameter space which stringently determines the BSR property. In such directions, the experimentally fitted parameter values were included in the range of the obtained parameter sets. The arachidonic acid metabolic pathway model was used as a model related to pharmacological responses. The pharmacological effects of nonsteroidal anti-inflammatory drugs were simulated using the parameter sets obtained by TEAPS. The structural properties of the system were partly extracted by analyzing the distribution of the obtained parameter sets. In addition, the simulations showed inter-drug differences in prostacyclin to thromboxane A2 ratio such that aspirin treatment tends to increase the ratio, while rofecoxib treatment tends to decrease it. These trends are comparable to the clinical observations. These results on real biological models suggest that the parameter sets satisfying the BSR condition can help in finding biologically plausible parameter sets and understanding the properties of biological systems.
Journal Article