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Today’s cities are facing the challenges of increasing traffic congestion, emissions, and the need to improve road safety. The solution to these problems is the use of artificial intelligence (AI) and the Internet of Things (IoT) in intelligent traffic management. The purpose of the article is to analyze and evaluate AI- and IoT-based solutions implemented in Polish cities and to identify innovative proposals that can improve traffic management. The study uses a mixed-method approach, including the analysis of crowdsourced mobility data (from GPS, smartphones, and municipal reports), GIS tools for mapping congestion, big data analytics, and machine learning algorithms, to evaluate trends and predict traffic scenarios. The evaluation focused on seven major Polish cities—Warsaw, Krakow, Wroclaw, Gdansk, Poznan, Katowice, and Lodz—where intelligent transportation systems such as dynamic traffic lights, intelligent pedestrian crossings, accident prediction systems, and parking space management have been implemented. The effectiveness of these solutions was assessed using the following six key indicators: waiting time at intersections, travel time, congestion level, CO2 emissions, energy consumption, and number of traffic incidents. The article provides a comprehensive analysis of these solutions’ impacts on traffic flow, emissions, energy efficiency, and road safety. A key contribution of the paper is the presentation of new proposals for improvements, such as the inclusion of behavioral data in traffic modeling, integration with GPS navigation, and dynamic emergency and public transport priority management. The article also discusses further digitization and interoperability needs. The findings show that the implementation of intelligent transportation systems not only improves urban mobility and safety but also enhances environmental sustainability and residents’ quality of life.

The aim of the article is to present and analyze the implementation of intelligent lighting within the concept of smart energies and smart cities. Motivation and research hypothesis: Electricity consumption in the world is based largely on non-renewable energy. Until these full changes, it is necessary to look for opportunities to save and use it efficiently. Today’s cities are increasingly implementing the smart concept, of which smart energy is one area. One of the smart city elements implemented by cities is smart energy. Within this framework, a supported concept is the replacement of traditional lighting with LEDs (Light-Emitting Diodes), which contributes to energy and cost savings and reduces the pollution of the sky with artificial light, while increasing the efficiency of urban lighting. Positive effects of modern solutions include reduced lighting expenses and increased safety of residents. Methods and results of the research: The authors chose the case study method for their research. The authors present forecasts for the development, not only from the point of view of the number of lamps but also the cost efficiency pointing out the importance of this element in the context of building smart cities. These are specific benchmarks for cities that have not yet implemented this concept. Conclusions and interdisciplinary implications: solutions are desirable directions for the development of the smart city concept, bringing benefits and reducing external costs. Considerations show a quantitative development forecast and an indication of the possibility of achieving trade-offs and cost reductions. It translates into meeting the requirements of sustainable development providing tangible benefits. The analysis of the case studies is intended to show the effects that can be achieved and the wide range of applications (indicating that modern lamps are not just lighting, but a platform for urban services). The analyses presented are intended to serve as benchmarks showing the possibilities of reducing costs and increasing the quality of life of residents in modern cities. The indicated examples and analyses are of economic importance, they show managers, but also inhabitants, in which direction they should lead the smart city concept, which will allow saving costs, but also to increase the quality of life of inhabitants.

The aim of the given paper is to present the analysis of tourism services by means of time series models and forecasting of evaluation of tourism services. Tourism services are analysed according to various parameters: hotel stay price, hotel services quality (such as cleanness of rooms, check-in, information provision etc.), catering quality and medical service quality. The research has been undertaken on the basis of responses of foreign guests of 13 hotels in Kołobrzeg region taken from 1400 questionnaires divided according to age and sex of respondents. Various econometric models were used for the analysis of statistic regularities. First, customers evaluated quality of their stay in hotels. These data were examined during the 2006-2009 time period as a stochastic process. It was found that the processes are nonstationary, that is why the ARIMA (1,1,1) model was used in the study. On the basis of the analyses and prognoses one can deduce that models of time series make it possible to estimate a tendency that occurs for an analysed parameter, however misprediction is quite possible to appear (up to 30 %). Similar results were achieved during the analysis of evaluation of hotel stay quality on the basis of sex of respondents. One should point out that an average value of male evaluation rate is higher than an average value of female evaluation rate. Time series for analysed variables were integrated into level I(1). A certain co-integrational connection was found between the evaluation of hotel stay and number of stays in a given hotel, where tourists that have already visited a given hotel for several times presumably give a high evaluation rate of hotel stay quality. The result of the analysis of long-term relation between hotel stay and duration of check-in is presented in high mutual dependence of hotel evaluation rate on evaluation of check-in duration. A hotel stay price influences hotel stay evaluation in a negative way, but catering and medical services are considered as standard and do not have any particular influence on hotel stay evaluation. The undertaken study shows that methods that take into account time series can be successfully used in analysis of parameters of tourist comfort and in evaluation of hotel services. Celem niniejszej pracy jest analiza jakości świadczonych usług w zależności od szeregu czynników: ceny pobytu w hotelu, jakości usług hotelarskich (czystość w pokoju, rejestracja pobytu, informowanie klientów itp.), jakości usług gastronomicznych (oferty gastronomiczne), ilości i jakości usług sanatoryjno-rehabilitacyjnych z wykorzystaniem metod szeregów czasowych oraz prognozowania oceny jakości świadczonych usług. Badania były przeprowadzone na podstawie ankietowania gości zagranicznych z 13 hoteli regionu kołobrzeskiego, razem powyżej 1400 ankiet w zależności od wieku i płci ankietowanych. Dla ustalenia prawidłowości statystycznych zastosowano różne modele ekonometryczne. Po pierwsze, ocenianie jakości pobytu w hotelach przez klientów było rozpatrywane w przestrzeni czasowej 2006-2009 jako proces stochastyczny. Ustalono, że procesy są niestacjonarne, dlatego badanie bylo przeprowadzone na podstawie modelu ARIMA(1,1,1). Badania i prognozy pozwalają zrobić wniosek, że modele szeregów czasowych mogą oszacować trend, występujący dla badanego wskaźnika, natomiast błąd prognozowania jest dość wysoki (do 30%). Podobne wyniki były otrzymane rownież podczas badania ocen jakości pobytu w hotelach w zależności od płci badanych. Zaznaczymy, że średnia wartość ocen mężczyzn jest wyższa niż średnia ocen kobiet. Szeregi czasowe dla badanych zmiennych miały stopien zintegrowania I(1). Została ustalona znaczna więź integracyjna między oceną pobytu w hotelu oraz ilością odwiedzin wybranego hotelu, kiedy turyści nie po raz pierwszy odwiedzający hotel przeważnie wystawiają wysokie oceny jakości pobytu w hotelu. Wynikiem badania zależności długookresowej pobytu w hotelu od czasu trwania procedury zameldowania jest bardza wysoka współzależność oceny pobytu od oceny czasu trwania procedury zameldowania. Cena pobytu w hotelu negatywnie wpływa na ocenę pobytu w hotelu, natomiast usługi sanatoryjne i gastronomiczne odbierane są jako standard i nieznacznie wpływają na ocenę pobytu w hotelu. Przeprowadzone badania wskazują, że metody szeregów czasowych mogą być skutecznie zastosowane w badaniu wskaźników turystycznych i ocenie jakości usług hotelarskich.

Acrylamide (ACR) is an amide formed as a byproduct in many heat-processed starchy-rich foods. In utero ACR exposure has been associated with restricted fetal growth, but its effects of postnatal functional development of small intestine is completely unknown. The current study investigated the time- and segment-dependent effects of prenatal ACR exposure on morphological and functional development of small intestine in weaned rat offspring. Four groups of pregnant female Wistar rats were exposed to ACR (3 mg/kg b.w./day) for 0, 5, 10 and 15 days during pregnancy. Basal intestinal morphology, immunolocalization of gut hormones responsible for food intake and proteins of intestinal barrier, activity of the intestinal brush border disaccharidases, apoptosis and proliferation in intestinal mucosa were analyzed in offspring at weaning (postnatal day 21). The results showed that in utero ACR exposure disturbs offspring gut structural and functional postnatal development in a time- and segment-depended manner and even a short prenatal exposure to ACR resulted in changes in intestinal morphology, immunolocalization of leptin and ghrelin and their receptors, barrier function, activity of gut enzymes and upregulation of apoptosis and proliferation. In conclusion, prenatal ACR exposure disturbed the proper postnatal development of small intestine.

Osteosarcopenia, characterized by concurrent bone loss and muscle wasting, significantly impacts mobility and quality of life. While age-related primary osteosarcopenia is well-studied, secondary osteosarcopenia (SOS) caused by chronic diseases remains poorly understood, particularly in young individuals. The present study aimed to comprehensively characterize musculoskeletal alterations associated with SOS using a juvenile porcine model of cerulein-induced chronic pancreatitis. Femoral bone analysis included densitometry, mechanical testing, histomorphometry, and serum bone turnover markers. The quadriceps femoris muscle was evaluated through histological analysis and gene expression profiling of antioxidant enzymes and apoptotic regulators. Animals with SOS showed significantly reduced femoral BMD compared to controls, with altered cortical geometry and compromised mechanical properties. Trabecular bone analysis revealed classic osteoporotic changes with decreased bone volume fraction. Negative changes were also observed in the growth plate morphology, indicating impaired endochondral ossification. Bone turnover markers indicated elevated bone resorption and altered formation. Muscle analysis demonstrated sarcopenic changes with selective atrophy of fast-twitch type II fibers and increased fiber density. At the molecular level, SOS muscles exhibited downregulated expression of CAT and CASP3, suggesting muscle atrophy predominantly mediated by oxidative stress and caspase-independent proteolysis rather than classical apoptosis. In conclusion, chronic pancreatitis in young pigs induces coupled bone and muscle degeneration consistent with secondary osteosarcopenia, demonstrating that muscle–bone crosstalk dysfunction occurs early in chronic inflammatory disease.

Chronic pancreatitis (CP) in young individuals may lead to disease-related secondary sarcopenia (SSARC), characterized by muscle loss and systemic inflammation. In this study, CP was induced in young pigs, and serum levels of key hormones, muscle fiber diameters in various muscles, and the mRNA expression of genes related to oxidative stress and programmed cell death were assessed. A decrease in muscle fiber diameters was observed in SSARC pigs, particularly in the longissimus and diaphragm muscles. Hormonal analysis revealed alterations in dehydroepiandrosterone, testosterone, oxytocin, myostatin, and cortisol levels, indicating a distinct hormonal response in SSARC pigs compared to controls. Oxytocin levels in SSARC pigs were significantly lower and myostatin levels higher. Additionally, changes in the expression of catalase (CAT), caspase 8 (CASP8), B-cell lymphoma 2 (BCL2), and BCL2-associated X protein (BAX) mRNA suggested a downregulation of oxidative stress response and apoptosis regulation. A reduced BAX/BCL2 ratio in SSARC pigs implied potential caspase-independent cell death pathways. The findings highlight the complex interplay between hormonal changes and muscle degradation in SSARC, underscoring the need for further research into the apoptotic and inflammatory pathways involved in muscle changes due to chronic organ inflammation in young individuals.

Fumonisins (FB) are metabolites found in cereal grains (including maize), crop products, and pelleted feed. There is a dearth of information concerning the effects of FB intoxication on the intestinal histomorphometry, the expression of intestinal tight junction proteins, and the bone structure and liver in pre-laying hens. The current experiment was carried out on hens from the 11th to the 14th week of age. The hens were orally administered an extract containing fumonisin B1 (FB1) and fumonisin B2 (FB2) at doses of 0.0 mg/kg b.w. (body weight), 1.0 mg/kg b.w., 4.0 mg/kg b.w., and 10.9 mg/kg b.w. for 21 days. Following FB intoxication, the epithelial integrity of the duodenum and jejunum was disrupted, and dose-dependent degenerative changes were observed in liver. An increased content of immature collagen was observed in the bone tissue of FB-intoxicated birds, indicating intensified bone turnover. A similar effect was observed with regards to the articular cartilage, where enhanced fibrillogenesis was observed mainly in the group of birds that received the FB extract at a dose of 10.9 mg/kg b.w. In conclusion, FB intoxication resulted in negative structural changes in the bone tissue of the hens, which could result in worsened bone mechanics and an increase in the risk of bone fractures. Fumonisin administration, even at a dose of 1.0 mg/kg b.w., can lead to degradation of the intestinal barrier and predispose hens to intestinal disturbances later in life.

The potential effects of the prenatal administration of dexamethasone and the postnatal treatment with 2-oxoglutaric acid on postnatal development of the small intestine of farm animals have not been examined experimentally. The aim of this study was to establish the changes in morphologic parameters of the small intestine damaged by the prenatal action of dexamethasone in piglets supplemented with 2-oxoglutaric acid. Three milligrams dexamethasone was administered intramuscularly every second day from day 70 of pregnancy to parturition and then piglets were supplemented with 2-oxoglutaric acid for 35 d of postnatal life (0.4 g/kg of body weight). The histomorphometry of the pig duodenum and jejunum was determined. Immunohistochemical staining with anti–Ki-67, CD3, null T cells, cadherin, claudin, and neurofilament antibodies was performed. Maternal treatment with dexamethasone decreased and limited the expression of claudin and cadherin in the epithelium. Dexamethasone led to thinning of the myenteron of the duodenum and the middle part of the jejunum in weaned piglets and influenced duodenal glands that became more elongated compared with control glands. Moreover, 2-oxoglutaric acid increased cell proliferation and the amount and maturity of peripheral blood lymphocytes in the duodenum and jejunum. It supported epithelial integrity and changed the circularity of the nerve plexuses. The 2-oxoglutaric acid administered to piglets while suckling induced a complete recovery from intestinal damage caused by the prenatal action of dexamethasone.

In this study, we analyzed multibilayer lipid-protein membranes composed of the photosynthetic light-harvesting complex II (LHCII; isolated from spinach [Spinacia oleracea]) and the plant lipids monogalcatosyldiacylglycerol and digalactosyldiacylglycerol. Two types of pigment-protein complexes were analyzed: those isolated from dark-adapted leaves (LHCII) and those from leaves preilluminated with high-intensity light (LHCII-HL). The LHCII-HL complexes were found to be partially phosphorylated and contained zeaxanthin. The results of the x-ray diffraction, infrared imaging microscopy, confocal laser scanning microscopy, and transmission electron microscopy revealed that lipid-LHCII membranes assemble into planar multibilayers, in contrast with the lipid-LHCII-HL membranes, which form less ordered structures. In both systems, the protein formed supramolecular structures. In the case of LHCII-HL, these structures spanned the multibilayer membranes and were perpendicular to the membrane plane, whereas in LHCII, the structures were lamellar and within the plane of the membranes. Lamellar aggregates of LHCII-HL have been shown, by fluorescence lifetime imaging microscopy, to be particularly active in excitation energy quenching. Both types of structures were stabilized by intermolecular hydrogen bonds. We conclude that the formation of trans-layer, rivet-like structures of LHCII is an important determinant underlying the spontaneous formation and stabilization of the thylakoid grana structures, since the lamellar aggregates are well suited to dissipate excess energy upon overexcitation.