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CaWRKY40 was previously found to be transcriptionally up‐regulated by Ralstonia solanacearum inoculation (RSI) or heat stress (HS), but the underlying mechanism remains unknown. Herein, we report that a double‐W box‐element (DWE) in the promoter of CaWRKY40 is critical for these responses. The upstream W box unit WI of this composite element is crucial for preferential binding by CaWRKY40 and responsiveness to RSI or HS. DWE‐driven CaWRKY40 can be transcriptionally and nonspecifically regulated by itself and by CaWRKY58 and CaWRKY27. The DWE was also found in the promoters of CaWRKY40 orthologs, including AtWRKY40, VvWRKY40, GmWRKY40, CplWRKY40, SaWRKY40, SpWRKY40, NtWRKY40, and NaWRKY40. DWEAtWRKY40 was analogous to DWECaWRKY40 by responding to RSI or HS and AtWRKY40 expression. These data suggest that a conserved response of plants to pathogen infection or HS is probably mediated by binding of the DWE by WRKY40. Our study reports that a conserved response of plants to pathogen infection or heat shock is probably mediated by the binding of the double‐W box to WRKY40.

Hypertrophic scars are one of the most important complications in surgery due to their cosmetic and functional impairments. Previous studies in tissue fibrotic disorders have shown promising results by inhibiting the biological activity effect of Transforming Growth Factor-beta 1 (TGF-β1). The aim of the current study was to determine the clinical effect of the inhibition of TGF-β1 signaling in human hypertrophic scars implanted in nude mice by topical application of an inhibitor of TGF-β1 (P144®). A total of 30 human hypertrophic scars were implanted in 60 nude mice. The animals were divided in two groups, group A (placebo) and group B (treatment). Group C (basal) was considered as the preimplanted scar samples and they were not implanted in the nude mice. After the shedding period, topical application of a lipogel containing placebo (group A) or P144 (group B) was daily administered during two weeks. The animals were sacrificed upon completion of the study. Total area, thickness and collagen fibers area were measure and compared across all groups. Immunohistochemistry was also performed in order to quantify collagen type I and type III and elastic fiber expressions present in the dermis. Successful shedding was achieved in 83,3% of the xenografts. The mean time for shedding was 35±5.4 days. Statistically significant differences were found in the total area, collagen fibers area and thickness between the groups. Increased elastic fibers and decreased collagen I were found in the P144-treated group compared to the basal group. Topical application of an inhibitor of TGF-β1 may promote scar maturation and clinical improvement of hypertrophic scar morphology features in an \"in vivo\" model in nude mice after two weeks of treatment.

pH plays a pivotal role in influencing various aspects of proton-coupled electron transfer (PCET) reactions in electrochemical systems. These reactions are affected by pH in terms of mass transport, electrochemical double layer (EDL) structure, and surface adsorption energy, all of which impact the overall electrochemical processes. This review article aims to provide a comprehensive understanding of the research progress made in elucidating the effects of pH on different electrochemical reactions, the hydrogen evolution reaction/hydrogen oxidation reaction (HER/HOR), oxygen reduction reaction/oxygen evolution reaction (ORR/OER), and carbon dioxide reduction reaction (CO 2 RR). To embark on this endeavor, we have conducted a bibliometric analysis to clearly outline of the research trends and advancements in the field concerning the pH effects. Subsequently, we present a systematic overview of the mechanisms governing these reactions, with a special focus on pH’s influence on both the proton and electron aspects. We conclude by discussing the current challenges in this area and suggesting future research avenues that could further our understanding of pH's role in electrochemical reactions. Graphical abstract

This study aimed to translate and validate The Resilience Scale for Kidney Transplantation (RS-KTPL) into Chinese and assess its reliability and validity among kidney transplant patients in China. With authorization from the original authors, the RS-KTPL was translated following Brislin's translation model, including forward translation, back translation, author review, cross-cultural adaptation, and a pilot study, resulting in a Chinese version of the RS-KTPL. A total of 358 kidney transplant recipients were recruited through convenience sampling and completed the questionnaire. Statistical analyses included item analysis, content validity, structural validity, convergent validity, discriminant validity, and reliability. Item analysis led to the removal of certain items that did not meet the criteria, resulting in a final version of the scale with 22 items across four dimensions. For content validity, the item-level content validity index (I-CVI) ranged from 0.83 to 1.000, and the scale-level content validity index (S-CVI/Ave) was 0.91, indicating good content fit. Structural validity was confirmed through exploratory and confirmatory factor analyses, supporting a four-factor structure with a cumulative variance contribution rate of 64.913% and all factor loadings exceeding 0.5. Convergent and discriminant validity analyses showed that the composite reliability (CR) values ranged from 0.741 to 0.938, and the average variance extracted (AVE) values ranged from 0.5 to 0.704, with the square root of AVE being higher than the inter-factor correlation coefficients, indicating good internal consistency and discriminating ability. Reliability testing showed a Cronbach's α coefficient of 0.944 for the overall scale, with subscale Cronbach's α coefficients all above 0.696, and a split-half reliability of 0.891, demonstrating high internal consistency and stability of the scale. The Chinese version of the RS-KTPL exhibits good reliability and validity among kidney transplant patients in China and can be effectively used to assess psychological resilience in this population.

Background Spinal tuberculosis is a rare condition that can result in significant neurological impairments. It is typically classified into two main types based on the anatomical involvement: parenchymal and meningeal. However, existing studies have not provided systematic reports that clarify the differences in aetiology, pathophysiological mechanisms, and clinical presentations between these two types. Methods This retrospective study includes 107 patients diagnosed with intracranial tuberculosis accompanied by spinal tuberculosis via magnetic resonance imaging (MRI) between May 2019 and May 2024 at our hospital. Patients are categorised into parenchymal and meningeal types based on radiological manifestations. Clinical symptoms, comorbidities, peripheral blood and cerebrospinal fluid laboratory indices, and MRI data are collected for both groups. Statistical analyses are performed using Chi-square tests, independent sample t-tests and non-parametric tests. Results Parenchymal spinal tuberculosis is more frequently associated with haematogenous pulmonary dissemination, exhibits a stronger inflammatory response and is linked to poorer nutritional status. Meningeal spinal tuberculosis often co-occurs with tuberculous meningitis, with common symptoms including sensory and motor deficits and lumbar back pain. Further, MRI findings indicate that parenchymal tuberculosis predominantly features nodular lesions, whereas meningeal tuberculosis is characterised by dural linear or leptomeningeal enhancement. Conclusion Significant differences exist in the clinical and radiological manifestations of parenchymal and meningeal spinal tuberculosis, and MRI enhancement scans play a crucial role in diagnosis, aiding in the optimisation of diagnostic and treatment strategies.

By modulating the mass ratio of hydrothermal agents to cobalt/iron precursors, Co3O4 nanowires were successfully integrated into spinel-type Co/Fe@NF, forming a heterojunction anode for alkaline water electrolysis (AWE) hydrogen production. This Co3O4 nanowire-assembled CoFe2O4 nanosheet anode (Co/Fe(5:1)@NF) exhibits exceptional electrochemical oxygen evolution reaction (OER) performance, requiring only 221 mV overpotential to achieve 10 mA cm−2. Sulfamethoxazole (SMX) was employed as a model pollutant to investigate the anode sacrificial material; it achieved approximately 95% SMX degradation efficiency, reducing the OER potential of 50 mV/10 mA cm−2. SMX oxidation coupled with Co/Fe heterojunction structure partially substitutes the OER. Co/Fe heterojunction generates an internal magnetic field, which induces the formation of novel active species within the system. ·O2− is the newly formed active oxygen species, which enhanced the proportion of indirect SMX oxidation. Quantitative analysis reveals that superoxide radical-mediated indirect oxidation of SMX accounts for approximately 38.5%, Fe(VI) for 9.4%, other active species for 6.1%, and direct oxidation for 46.0%. The nanowire–nanosheet assembly stabilizes a high-spin configuration on the catalyst surface, redirecting oxygen intermediate pathways toward triplet oxygen (3O2) generation. Subsequent electron transfer from nanowire tips facilitates rapid 3O2 reduction, forming superoxide radicals (·O2−). This study effectively driven by indirect oxidation, with cathodic hydrogen production, providing a novel strategy for utilizing renewable electricity and reducing OER while offering insights into the design of Co/Fe-based catalyst.

Chirality is a prevalent characteristic of natural systems that plays a significant role in the biological activities of living organisms, and the enantiomers typically exhibit different pharmacological activities. Consequently, developing methods with high selectivity and sensitivity for chiral analysis is of great importance for pharmaceutical engineering, biomedicine, and food safety. Electrochemical chiral recognition has garnered significant attention owing to its unique advantages, including simplicity of operation, rapid response, and cost-effectiveness. The biomaterials, such as amino acids, proteins, nucleic acids, and polysaccharides, possess inherent chiral sites, excellent biocompatibility, and abundant modifiable groups, rendering them ideal candidates for constructing electrochemical chiral sensors. This review focuses on the research progress of electrochemical chiral recognition based on different biomaterials from 2019 to 2024. In addition, the distinct chiral recognition mechanisms and electrochemical analysis methods, as well as the research challenges and prospects of electrochemical chiral sensors based on biomaterials in enantiomer recognition are discussed. This review can provide a reference for further study in related fields.

Advancements in reconstructive microsurgery have evolved into supermicrosurgery; connecting vessels with diameter between 0.3 and 0.8 mm for reconstruction of lymphatic flow and vascularized tissue transplantation. Supermicrosurgery is limited by the precision and dexterity of the surgeon’s hands. Robot assistance can help overcome these human limitations, thereby enabling a breakthrough in supermicrosurgery. We report the first-in-human study of robot-assisted supermicrosurgery using a dedicated microsurgical robotic platform. A prospective randomized pilot study is conducted comparing robot-assisted and manual supermicrosurgical lymphatico-venous anastomosis (LVA) in treating breast cancer-related lymphedema. We evaluate patient outcome at 1 and 3 months post surgery, duration of the surgery, and quality of the anastomosis. At 3 months, patient outcome improves. Furthermore, a steep decline in duration of time required to complete the anastomosis is observed in the robot-assisted group (33–16 min). Here, we report the feasibility of robot-assisted supermicrosurgical anastomosis in LVA, indicating promising results for the future of reconstructive supermicrosurgery. Reconstructive microsurgery is limited by the precision that human hands can achieve. Here, the authors demonstrate in a randomized clinical pilot trial the feasibility of robot-assisted supermicrosurgery using a dedicated microsurgical robot for the completion of lymphatico-venous anastomosis in the treatment of breast cancer-related lymphedema

As the largest transporter family impacting on tumor genesis and development, the prognostic value of solute carrier (SLC) members has not been elucidated in colorectal cancer (CRC). We aimed to identify a prognostic signature from the SLC members and comprehensively analyze their roles in CRC. Firstly, we downloaded transcriptome data and clinical information of CRC samples from GEO (GSE39582) and TCGA as training and testing dataset, respectively. We extracted the expression matrix of SLC genes and established a prognostic model by univariate and multivariate Cox regression. Afterwards, the low-risk and high-risk group were identified. Then, the differences of prognosis traits, transcriptome features, clinical characteristics, immune infiltration and drug sensitivity between the two groups were explored. Furthermore, molecular subtyping was also implemented by non-negative matrix factorization (NMF). Finally, we studied the expression of the screened SLC genes in CRC tumor tissues and normal tissues as well as investigated the role of SLC12A2 by loss of function and gain of function. As a result, we developed a prognostic risk model based on the screened 6-SLC genes (SLC39A8, SLC2A3, SLC39A13, SLC35B1, SLC4A3, SLC12A2). Both in the training and testing sets, CRC patients in the high-risk group had the poorer prognosis and were in the more advanced pathological stage. What’s more, the high-risk group were enriched with CRC progression signatures and immune infiltration. Two groups showed different drug sensitivity. On the other hand, two distinct subclasses (C1 and C2) were identified based on the 6 SLC genes. CRC patients in the high-risk group and C1 subtype had a worse prognosis. Furthermore, we found and validated that SLC12A2 was steadily upregulated in CRC. A loss-of-function study showed that knockdown of SLC12A2 expression restrained proliferation and stemness of CRC cells while a gain-of-function study showed the contrary results. Hence, we provided a 6-SLC gene signature for prognosis prediction of CRC patients. At the same time, we identified that SLC12A2 could promote tumor progression in CRC, which may serve as a potential therapeutic target.

Purpose Breast cancer-related lymphedema (BCRL) is caused by an interruption of the lymphatic system after breast cancer treatment. Lymphaticovenous anastomosis (LVA), by which one or more patent lymphatic collecting vessels are connected to subcutaneous veins, shows promising results. Postoperatively, the patency of these anastomosis can be evaluated; however, little is known concerning the long-term patency after LVA in patients with BCRL. The aim of this study was to analyse the long-term patency, quality of life (QoL) and arm circumference after LVA, and to explore differences between patent and non-patent anastomosis and its correlation with clinical improvement. Methods Twenty-five patients underwent indocyanine green (ICG) lymphography, lymph ICF-questionnaire, and arm circumference measurement preoperatively and 12 months after the LVA procedure. Results Seventy-six percent of the patients showed at least one patent anastomosis after 12 months. Quality of life according to the Lymph-ICF increased significantly ( p  < 0.000); however, arm circumference showed no significant decrease. Sixty-five percent discontinued wearing compression stockings. The patent anastomosis group, compared with the non-patent anastomosis group showed, without significance, more improvement in QoL, arm circumference, and discontinuation of compression stockings, as well as a lower rate of infections both pre- and postoperatively, a shorter duration of lymphedema preoperatively, and a higher rate of early lymphedema and ICG stage. Conclusions LVA showed an acceptable patency and positive correlation between a patent anastomosis and clinical improvement after 12 months. Further research with a larger study population is required to determine whether outcomes or patient characteristics significantly correlate with a patent anastomosis after LVA operation.