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Perchlorate is a toxic, explosive, and water-soluble pollutant but while efficient and low-cost removal of ClO 4 − from water is an important challenge, suitable methods for precipitation of ClO 4 − from water are underdeveloped. Here, we demonstrate a hydrogen-bonded supramolecular polymer network crystal ( HBPC ) for efficient complexation of ClO 4 − . The HBPC network is constructed by an A,A’-bis-pyridyl-hydrazone-phenyl conjugate-functionalized pillar[5]arene ( PYP5 ), which self-assembles via the formation of clustered hydrogen-bonds. Two-pyridyl-hydrazone moieties provide coordination sites while the eight ethoxyl moieties on the pillar[5]arene enable multiple hydrogen bonding motifs to tightly bind to the ClO 4 − . The HBPC network therefore adsorbs ClO 4 − ions and facilitates the separation of these ions from water. Perchlorate is a toxic, explosive pollutant, and is difficult to precipitate due to its water solubility. Here, the authors report a pillar[5]arene-based supramolecular polymer network that can bind with perchlorate by clustered hydrogen-bonds for removal from water.

Background Mitochondrial fission regulator 2 (MTFR2) was involved in the progression and development of various cancers. However, the relationship between MTFR2 with lung adenocarcinoma (LUAD) had not been reported. Herein, this study analyzed the clinical significance and potential mechanisms of MTFR2 in LUAD via bioinformatics tools. Results We found that the level of MTFR2 was increased, and correlated with sex, age, smoking history, neoplasm staging, histological subtype and TP53 mutation status in LUAD patients. Kaplan-Meier survival analysis showed LUAD patients with increased MTFR2 had a poor prognosis. In addition, univariate COX regression analysis showed neoplasm staging, T stage, distant metastasis and MTFR2 level were risk factors for the prognosis of LUAD. A total of 1127 genes were coexpressed with MTFR2, including 840 positive and 208 negative related genes. KEGG and GSEA found that MTFR2 participated in the progression of LUAD by affecting cell cycle, DNA replication, homologous recombination, p53 signaling pathway and other mechanisms. The top 10 coexpressed genes, namely CDK1, CDC20, CCNB1, PLK1, CCNA2, AURKB, CCNB2, BUB1B, MAD2L1 and BUB1 were highly expressed, and were associated with poor prognosis in LUAD. Conclusions Consequently, we elucidated MTFR2 was a biomarker for diagnosis and poor prognosis in LUAD, and might participate in the progression of LUAD via affecting cell cycle, DNA replication, homologous recombination and p53 signaling pathway.

Organoids hold inestimable therapeutic potential in regenerative medicine and are increasingly serving as an in vitro research platform. Still, their expanding applications are critically restricted by the canonical culture matrix and system. Synthesis of a suitable bioink of bioactivity, biosecurity, tunable stiffness, and printability to replace conventional matrices and fabricate customized culture systems remains challenging. Here, we envisaged a novel bioink formulation based on decellularized extracellular matrix (dECM) from porcine small intestinal submucosa for organoids bioprinting, which provides intestinal stem cells (ISCs) with niche‐specific ECM content and biomimetic microstructure. Intestinal organoids cultured in the fabricated bioink exhibited robust generation as well as a distinct differentiation pattern and transcriptomic signature. This bioink established a new co‐culture system able to study interaction between epithelial homeostasis and submucosal cells and promote organoids maturation after transplantation into the mesentery of immune‐deficient NODSCID‐gamma (NSG) mice. In summary, the development of such photo‐responsive bioink has the potential to replace tumor‐derived Matrigel and facilitate the application of organoids in translational medicine and disease modeling.

Amendment significantly improves soil structure and promotes crop growth. To combat soil degradation and low crop yields in facility agriculture, it is crucial to study the optimal application rate of amendments. This study analyzed the effects of biochar, vermicompost, and mineral-source potassium fulvic acid on the stability of aggregate structure, soil nutrient content, and tomato yield in cambisols, providing a theoretical basis for improving the soil quality of plastic greenhouses in Southern China. A pot experiment on tomato cultivation was carried out in yellow-brown soil in plastic greenhouses. The experiment included eight treatments: 1% biochar (B1); 3% biochar (B3); 5% biochar (B5); 3% vermicompost (V3); 5% vermicompost (V5); 0.1% mineral-source potassium fulvic acid (F1); 0.2% mineral-source potassium fulvic acid (F2); and the control condition without adding soil amendments (CK). The results showed that the biochar and vermicompost treatments effectively reduced soil bulk density and increased total soil porosity. Compared to the control, treatments with soil amendments significantly increased soil pH and had different effects on soil nutrients: F2 showed the most significant improvement in the content of available nitrogen, available phosphorus, and available potassium, with an increase of 133.33%, 834.59%, and 74.34%, respectively; B3 treatment had the highest increase in dissolved organic carbon (DOC), while B5 treatment had the highest organic matter content. Compared to the CK, the particle size of the biochar treatment was mainly 0.053~0.25 mm, while the V3, F1, and F2 mainly occurred with a particle size > 0.25 mm; and V3 has the best aggregate stability. Biochar, vermicompost, and mineral potassium fulvic acid can all promote tomato yield, with the F2 and V3 treatments having a yield increase effect of over 30%. Furthermore, Pearson’s correlation analysis showed a highly significant positive correlation between geometric mean diameter (GMD) and mean weight diameter (MWD), water-stable macroaggregate content (R0.25), and a positive correlation between alkaline-dissolved nitrogen, available phosphorus, dissolved organic carbon content, and aggregate stability indicators. Adding 0.2% mineral-source potassium fulvic acid optimizes cambisols’ properties, enhances aggregate formation and stability, boosts tomato yield, and shows great application potential.

With the advancement of digital streaming technology, multi-modal recommendation systems have gained significant attention. Current graph-based multi-modal recommendation approaches typically model user interests using either user interaction signals or multi-modal item information derived from heterogeneous graphs. Although methods based on graph convolutional networks (GCNs) have achieved notable success, they still face two key limitations: (1) the narrow interpretation of interaction information, leading to incomplete modeling of user behavior, and (2) a lack of fine-grained collaboration between user behavior and multi-modal information. To address these issues, we propose a novel method by decomposing interaction information into two distinct signal pathways, referred to as a dual-path selection architecture, named Dual-path Selective Graph Recommender (DSGRec). DSGRec is designed to deliver more accurate and personalized recommendations by facilitating the positive collaboration of interactive data and multi-modal information. To further enhance the represetation of these signals, we introduce two key components: (1) behavior-aware multimodal signal augmentation, which extract rich multimodal semantic information; and (b) hypergraph-guided cooperative signal enhancement, which captures hybrid global information. Our model learns dual-path selection signals via a primary module and introduces two auxiliary modules to adjust these signals. We introduce independent contrastive learning tasks for the auxiliary signals, enabling DSGRec to explore the mechanisms behind feature embeddings from different perspectives. This approach ensures that each auxiliary module aligns with the user-item interaction view independently, calibrating its contribution based on historical interactions. Extensive experiments conducted on three benchmark datasets demonstrate the superiority of DSGRec over several state-of-the-art recommendation baselines, highlighting the effectiveness of our method.

Background Emerging evidence indicates that reactive microglia-initiated inflammatory responses are responsible for secondary damage after primary traumatic spinal cord injury (SCI); epidermal growth factor receptor (EGFR) signaling may be involved in cell activation. In this report, we investigate the influence of EGFR signaling inhibition on microglia activation, proinflammatory cytokine production, and the neuronal microenvironment after SCI. Methods Lipopolysaccharide-treated primary microglia/BV2 line cells and SCI rats were used as model systems. Both C225 and AG1478 were used to inhibit EGFR signaling activation. Cell activation and EGFR phosphorylation were observed after fluorescent staining and western blot. Production of interleukin-1beta (IL-1β) and tumor necrosis factor alpha (TNFα) was tested by reverse transcription PCR and ELISA. Western blot was performed to semi-quantify the expression of EGFR/phospho-EGFR, and phosphorylation of Erk, JNK and p38 mitogen-activated protein kinases (MAPK). Wet-dry weight was compared to show tissue edema. Finally, axonal tracing and functional scoring were performed to show recovery of rats. Results EGFR phosphorylation was found to parallel microglia activation, while EGFR blockade inhibited activation-associated cell morphological changes and production of IL-1β and TNFα. EGFR blockade significantly downregulated the elevated MAPK activation after cell activation; selective MAPK inhibitors depressed production of cytokines to a certain degree, suggesting that MAPK mediates the depression of microglia activation brought about by EGFR inhibitors. Subsequently, seven-day continual infusion of C225 or AG1478 in rats: reduced the expression of phospho-EGFR, phosphorylation of Erk and p38 MAPK, and production of IL-1β and TNFα; lessened neuroinflammation-associated secondary damage, like microglia/astrocyte activation, tissue edema and glial scar/cavity formation; and enhanced axonal outgrowth and functional recovery. Conclusions These findings indicate that inhibition of EGFR/MAPK suppresses microglia activation and associated cytokine production; reduces neuroinflammation-associated secondary damage, thus provides neuroprotection to SCI rats, suggesting that EGFR may be a therapeutic target, and C225 and AG1478 have potential for use in SCI treatment.

Radical-involved allylation reactions have emerged as a powerful platform for construction of carbon-carbon and carbonheteroatom bonds, facilitating the strategic incorporation of diverse allyl moieties. Nevertheless, this burgeoning field still faces ongoing challenges, including limitations of radical precursors and coupling partners, and difficulties in achieving enantiocontrol. Herein, we report for the first time a highly enantioselective radical allylation involving β -keto esters with vinyl cyclopropanes utilizing a synergistic dual photoredox/nickel catalysis under visible light irradiation. The mild and redox-neutral catalytic protocol demonstrates an extensive substrate compatibility and good functional tolerance, providing access to enantioenriched β -keto esters featuring quaternary α -stereocenter with good yields and high enantioselectivities. Preliminary mechanistic studies have uncovered that the success of the reaction hinges on the dual roles of nickel catalyst, including in situ formation of photoredox sensitive substrate/Ni complex and the ensuing asymmetric radical addition step.

This study aimed to identify N-acylhomoserine lactone (AHL) produced by Hafnia alvei H4, which was isolated from spoiled instant sea cucumber, and to investigate the effect of AHLs on biofilm formation. Two biosensor strains, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens KYC55, were used to detect the quorum sensing (QS) activity of H. alvei H4 and to confirm the existence of AHL-mediated QS system. Thin layer chromatography (TLC) and high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS) analysis of the AHLs extracted from the culture supernatant of H. alvei H4 revealed the existence of at least three AHLs: N-hexanoyl-l-homoserine lactone (C6-HSL), N-(3-oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and N-butyryl-l-homoserine lactone (C4-HSL). This is the first report of the production of C4-HSL by H. alvei. In order to determine the relationship between the production of AHL by H. alvei H4 and bacterial growth, the β-galactosidase assay was employed to monitor AHL activity during a 48-h growth phase. AHLs production reached a maximum level of 134.6 Miller unites at late log phase (after 18 h) and then decreased to a stable level of about 100 Miller unites. AHL production and bacterial growth displayed a similar trend, suggesting that growth of H. alvei H4 might be regulated by QS. The effect of AHLs on biofilm formation of H. alvei H4 was investigated by adding exogenous AHLs (C4-HSL, C6-HSL and 3-oxo-C8-HSL) to H. alvei H4 culture. Biofilm formation was significantly promoted (p < 0.05) by 5 and 10 µM C6-HSL, inhibited (p < 0.05) by C4-HSL (5 and 10 µM) and 5 µM 3-oxo-C8-HSL, suggesting that QS may have a regulatory role in the biofilm formation of H. alvei H4.

Perovskite oxide has good sensitivity to VOCs gases, but its gas-sensitive properties are negatively affected by high humidity, thereby limiting its potential application in respiratory gas detection. This study focuses on synthesizing porous LaFeO 3 of perovskite type used a dual-process method involved hydrothermal synthesis with thermal treatment. The morphology and evolution of porous LaFeO 3 microspheres was studied. The LaFeO 3 sensors exhibited p-type gas-sensitive behavior with notable selectivity for respiratory gases. The LaFeO 3 sensor represented a significant response value of 9.7 to a 100 ppm C 2 H 5 OH concentration, with a rapid response/recovery time of 8 s/9 s at 300 °C. The sensor demonstrated a high response ( S  = 4.2) and a low detection limit of 100 ppb at 90% relative humidity. The superior sensor performance was due to the porous surface structure of LaFeO 3 microspheres, which improved the gas diffusion channels and reaction sites with microspheres. The density of states map revealed a strong orbital hybridization between C 2 H 5 OH and LaFeO 3 , resulted in a calculated adsorption energy of − 1.18 eV, signified a strong adsorption affinity in line with the experimental results. Graphical abstract

Growing evidence indicates that handgrip strength (HGS) is a conspicuous marker for assessing some diseases affecting middle-aged and elderly individuals. However, research regarding HGS and heart failure (HF) is sparse and controversial. Hence, we aimed to investigate the association between HGS and HF among adults aged 45 years and older in the United States. In this cross-sectional study, we included 4524 adults older than 45 years who were part of the National Health and Nutrition Examination Survey. A generalized additive model was used to estimate the association between HGS and HF. Age, gender, race, income, education, body mass index, smoking status, drinking status, diabetes, hypertension, stroke, vigorous physical activity, total energy intake, total protein intake, total sugars intake, and total fat intake covariates were adjusted using multiple regression models. And further subgroup analysis was conducted. We documented 189 cases of HF, including 106 men and 83 women. HGS was negatively associated with HF after adjusting for all the covariates (odds ratio = 0.97, 95% confidence interval = 0.96–0.99; P  < 0.001). Compared with the lowest quintile, the highest quintile was associated with an 82% lower incidence of HF (odds ratio = 0.18, 95% confidence interval = 0.08–0.43; P  < 0.001). Subgroup analysis showed that the results remained stable. In US adults older than 45, HGS was negatively associated with HF after adjusting for covariates. This finding had the potential to draw attention to the physiological and pathological effects of decreased muscle function on HF and may influence further prospective studies with intervention trials.