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To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed. Calcium chloride (CaCl 2 ) concentration (0.3–3.5%), alginate to gelling solution ratio (1:2–1:6); acetic acid (CH 3 COOH) concentration (0.2–5.0%) and alginate to gelling solution ratio (1:2–1:6) were taken as independent variables for EG and IG respectively. Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions. The optimized EG conditions were 0.3% CaCl 2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH 3 COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG. When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer. The Peppas–Sahlin model presented the best fit to experimental data. Polyphenol release was driven by diffusion, whereas bixin showed anomalous release. These results are promising for application as modulated release agents in food matrices.

Sonicated protein isolates were recovered from Chenopodium quinua, Phaseoulus vulgaris and Lens culinaris to develop a functional matrix by assessing the physicochemical and functional properties. The plant protein isolates were prepared from powdered materials followed by sonication in alkaline medium using a Box-Behnken design. pH (6–10), a buffer-to-material ratio (5:1 to 15:1) and sonication time (0–20 min) were taken as independent variables, whereas protein yield was taken as the dependent variable. A pH of 9, 20 min treatment, and a buffer-to-material ratio of 5:1 were the optimal extraction conditions for quinoa and black beans, whereas a 1:10 ratio was suitable for lentils. Sonication in alkaline medium caused partial protein unfolding and these isolates; in turn, the molecular weight affected the emulsifying activity and stability. Moreover, sonication had a strong effect on the gelation temperature, emulsifying activity, the water, and oil sorption. Sonication improved protein yield and exposed amino acids such as glutamic acid, aspartic acid, leucine and glycine. In turn, thiol groups were responsible for the increased in gelation temperature. The better gelling property coupled with high emulsifying property of these proteins show potential application as protein emulsifiers in the production of gels, sausages, and pet foods.

This study aimed to assess the thermal stability of the bioactive compounds from annatto seed extract, encapsulated by ionic gelation using quinoa proteins, lentil proteins, soy proteins, and sodium caseinate as carrying materials. The 10.0% aqueous dispersions of the different proteins (carriers) were prepared and mixed with the annatto seed extract. The dispersions were then extruded into a calcium chloride solution to induce the extract encapsulation. The capsules were characterized by encapsulation efficiency, particle size, infrared transmission spectroscopy, confocal microscopy, and scanning electron microscopy (SEM). The antioxidant and antimicrobial activities, the polyphenol compounds, and bixin content from the free and encapsulated extract were assessed once stored for 12 d at different temperatures (4 °C, 25 °C, and 65 °C). The results demonstrated the ability of the proteins to encapsulate the annatto extract with encapsulation efficiencies ranging from 58% to 80%, where the protein structure and amino acid content were the relevant factors to obtain high encapsulation efficiencies. The free extracts stored at 65 °C for 12 d experienced a degradation of bixin and polyphenol compounds, respectively. Conversely, the encapsulated extract had degradations from ~34.00% to ~4.05% for polyphenol compounds and ~20.0% for bixin, respectively. These proteins have a potential encapsulation capacity of annatto extract by ionic gelation.

This project studied the use of lentil protein (LP) and quinoa protein (QP) in their native and modified states as carrier material in the encapsulation process by the ionic gelation technique of annatto seed extract. Soy protein (SP) was used as a model of carrier material and encapsulated bioactive compounds, respectively. The plant proteins were modified by enzymatic hydrolysis, N acylation, and N-cationization to improve their encapsulating properties. Additionally, the secondary structure, differential scanning calorimetry (DSC), solubility as a function of pH, isoelectric point (pI), molecular weight (MW), the content of free thiol groups (SH), the absorption capacity of water (WHC) and fat (FAC), emulsifier activity (EAI), emulsifier stability (ESI), and gelation temperature (Tg) were assessed on proteins in native and modified states. The results obtained demonstrated that in a native state, LP (80.52% and 63.82%) showed higher encapsulation efficiency than QP (73.63% and 45.77%), both for the hydrophilic dye and for the annatto extract. Structural modifications on proteins improve some functional properties, such as solubility, WHC, FAC, EAI, and ESI. However, enzymatic hydrolysis on the proteins decreased the gels’ formation, the annatto extract’s encapsulated efficiency, and the hydrophilic dye by the ionic gelation method. On the other hand, the modifications of N-acylation and N-cationization increased but did not generate statistically significant differences (p-value > 0.05) in the encapsulation efficiency of both the annatto extract and the hydrophilic dye compared to those obtained with native proteins. This research contributes to understanding how plant proteins (LP and QP) can be modified to enhance their encapsulating and solubility properties. The better encapsulation of bioactive compounds (like annatto extract) can improve product self-life, potentially benefiting the development of functional ingredients for the food industry.

Goldenberry and purple passion fruit contain bioactive compounds (BCs) that can prevent gastrointestinal cancers; hydrogel beads can protect and control their release in the gastrointestinal tract. This study aimed to develop an encapsulating material for fruit hydrogel beads (FHBs) to increase their bioaccessibility and to assess antiproliferative effects. A blend of goldenberry–purple passion fruit was encapsulated using ionic gelation and electrospraying. Through a mixture experimental design with sodium alginate (SA), hydroxypropylmethylcellulose (HPMC) and arabic gum (AG) as components, the following response variables were optimized: polyphenol bioaccessibility and encapsulation efficiency. Polyphenols and antioxidant activity were quantified before and after digestion. Antiproliferative effect was evaluated on Caco-2 colon cancer cells. Variations in formulation proportions had a significant effect (p < 0.05) on most responses. An SA-AG mixture in a 0.75:0.25 ratio maximized polyphenol bioaccessibility to 213.17 ± 19.57% and encapsulation efficiency to 89.46 ± 6.64%. Polyphenols and antioxidant activity were lower in FHBs than in the fruit blend (F). Both F and FHBs inhibited tumor cell proliferation by 17% and 25%, respectively. In conclusion, encapsulating BCs in hydrogel beads with SA-AG can enhance the effectiveness of polyphenols in food applications by improving their bioaccessibility and showing a more pronounced effect in inhibiting tumor cell proliferation.

Proteins are macromolecules exhibiting amphiphilic properties, good biocompatibility, biodegradability, high nutritional value, and show strong interactions with several types of active compounds via hydrogen bonding and electrostatic interactions (1). These plant or animal-derived macromolecules differ in their molecular size depending on the number of amino acids present in their structure, which in turn, are linked by peptide bonds between the carbonyl (-CO-) and amino groups (-NH) (2).

ABSTRACT Background Creatine, widely recognized for its benefits in sports nutrition, is being explored in food applications to diversify its use and develop functional products. Here, we present preliminary data as part of The KreaFood Project, a research and innovation project conceptualized by the DBSS Research Division and co-developed with the Center for Advanced Studies in Nutrition and Food – CESNUTRAL at Universidad CES. Methods We assessed the potential of KreaFood products using Creavitalis®, an ultra-micronized creatine monohydrate powder, for developing creatine-fortified beverages and solid foods. Its performance was compared to regular creatine monohydrate in two food matrices: a mocaccino premix and a pancake premix. To prepare the mocaccino, 30 g (10% creatine) of the KreaFood premix was dissolved in 300 mL of warm water and stirred until homogeneous. For the solid food, 35 g of the KreaFood pancake premix (containing 10% creatine) was dissolved in 60 mL of milk and then cooked over low heat for one minute on each side or until golden brown. Quantitative descriptive sensory testing was performed on the KreaFood products using a double-blind panel of 15 trained evaluators. Additionally, rheological analysis was conducted for the mocaccino, while texture profile analysis (TPA) was used to assess the pancakes. We assessed the potential of KreaFood products using Creavitalis®, an ultra-micronized creatine monohydrate powder, for developing creatine-fortified beverages and solid foods. Its performance was compared to regular creatine monohydrate in two food matrices: a mocaccino premix and a pancake premix. To prepare the mocaccino, 30 g (10% creatine) of the KreaFood premix was dissolved in 300 mL of warm water and stirred until homogeneous. For the solid food, 35 g of the KreaFood pancake premix (containing 10% creatine) was dissolved in 60 mL of milk and then cooked over low heat for one minute on each side or until golden brown. Quantitative descriptive sensory testing was performed on the KreaFood products using a double-blind panel of 15 trained evaluators. Additionally, rheological analysis was conducted for the mocaccino, while texture profile analysis (TPA) was used to assess the pancakes. Results Creavitalis® was characterized by its smaller particle size and thermal stability, which facilitates more homogeneous dispersion and enhanced sensory texture in fortified food applications. In the mocaccino, sensory analysis revealed significant differences in granularity (P = 0.049), with lower granularity observed in the KreaFood product. In fact, preference for the KreaFood mocaccino was 46.7% (7/15), making it the option when a sweeter and creamier profile with a good chocolate aroma is desired. For pancakes, TPA tests showed that the KreaFood pancake exhibited higher cohesiveness (0.8255), resulting in a more uniform and smoother texture. Conversely, other commercial creatines displayed greater hardness (187.739 N) and gumminess (177.44), which translated into a less favorable sensory perception due to increased granularity. The superior cohesiveness observed in the KreaFood pancake product was attributed to its ability to integrate efficiently into the solid matrix during cooking. Our pancake premix was preferred by 53.3% (8/15) and demonstrated a more favorable sensory profile in terms of foaminess, odor intensity, and sweet taste, which are attributes generally desirable in solid foods. Conclusion The KreaFood creatine-fortified foods using ultra-micronized creatine monohydrate (Creavitalis®) demonstrated significant advantages by improving cohesiveness and reducing perceived granularity, thereby optimizing the sensory quality of the final product. The KreaFood project continues with validation in larger samples and testing of new applications in cappuccino, kumis, cereal bars, and ice cream. Disclosures DAB serves as the Scientific and Managing Director of KreaFood, an R&D&I project, and is a member of the “Creatine for Health” scientific advisory board for Alzchem Group AG. He leads the CREAS project by DBSS (available at: http://CREAS.pro/).

This study evaluated the antimicrobial activity (i.e., against Bacillus cereus and Staphylococcus aureus) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2–1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 μM trolox/L), and a minimal inhibitory concentration against Bacillus cereus and Staphylococcus aureus of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.

This study evaluated the impact of hydrodynamic cavitation (HC) versus conventional thermal processing (TT) for the valorization of fruit and vegetable surpluses, using optimized purees of carrot, banana, yacón, beetroot, and gulupa. HC-treated purees consistently preserved bioactive compounds, with vitamin C retention in purple carrot puree reaching 6.8 ± 0.6 mg/100 g, compared to only 0.6 ± 0.0 mg/100 g after thermal treatment. Total polyphenol content and antioxidant capacity (FRAP up to 2580 ± 126 μmol Eq-Trolox/100 g, DPPH inhibition up to 88.72% ± 0.80) were similarly superior with HC. While HC resulted in noticeably higher grumosity and fibrosity, limiting acceptance, TT improved sensory sweetness but degraded nutritional quality, causing up to 80% losses of vitamin C and bioactives. The findings confirm that HC is an effective non-thermal strategy for converting agri-food surpluses into functional ingredient bases, maximizing nutritional retention and energetic efficiency and supporting sustainable circular food systems.

This study deals with the extraction, optimization, and evaluation of the antioxidant and antimicrobial activities of bioactive compounds obtained from the seeds of annatto using microwave-assisted extraction as compared to leaching. Annatto seeds were subjected to a microwave treatment of 2450 MHz and power of 700 watts using a response surface design involving four factors: pH (4–11), solvent concentration (ethanol) (50–96%), solvent-to-seed ratio (2–10), and microwave exposure time (0–5 min). The contents of polyphenol compounds and bixin were taken as response variables. Subsequently, the antioxidant and antimicrobial activities were assessed at the optimal processing conditions predicted by the experimental design. Microwaves, solvent concentration, and the solvent-to-seed ratio showed a statistically significant effect for the extraction of polyphenol compounds and bixin. Thus, microwaves accelerated the extraction of those compounds and the slight increase in temperature caused some degradation of the polyphenol compounds. The microwave-assisted extraction increased the contents of polyphenols and bixin along with their antioxidant activity as compared to leaching extraction. However, this technique does not significantly improve the antimicrobial activity against Bacillus cereus and Staphylococcus aureus.