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Nile tilapia is recognized as a suitable candidate for intensive farming and sustainability of the aquaculture industry. However, one issue limiting Nile tilapia expansion in arid and semi-arid areas is the scarcity of freshwater resources. In this study, the supplementation of synbiotics was investigated to enhance the growth performance, growth-related genes, intestinal health, and immunity of Nile tilapia reared in inland brackish groundwater. Four diets were prepared where the basal diets were mixed with the dietary mixture of probiotics and prebiotics (Synbiotic Lactic Dry , a blend of , , , and , mannan oligosaccharides and β-1.3/1.6-D-glucan) at 0, 0.5, 1, and 2 g/kg. After eight weeks, the final weight and weight gain are linearly increasing with increasing the supplementation level of synbiotic. Markedly fish fed 0.5, 1, and 2 g/kg of synbiotic had higher final weight, weight gain, and feed intake and lower feed conversion ratio (FCR) than fish fed synbiotic free diet. The specific growth rate (SGR) was significantly higher in fish fed 1 and 2 g/kg than in fish fed 0 and 0.5 g/kg. The intestine of fish fed on synbiotic shows an increase in intestinal villi density. Further, the intestine of fish fed on synbiotic showed an increase in the length and branching intestinal villi (anterior, middle, and posterior) in a dose-dependent manner. The lysozyme and phagocytic activities were significantly different from the control, while synbiotic supplementation did not affect the phagocytic index. Interestingly, the results showed marked upregulation of ghrelin, IGF-1, and GH genes in fish fed synbiotics at 0.5, 1, and 2 g/kg. In addition, fish fed 2 g/kg had the highest expression of ghrelin, IGF-1, and GH genes. In conclusion, growing Nile tilapia in inland brackish groundwater can be achieved without negative impacts on the growth performance and health status. Supplementing synbiotics (1–2 g/kg) in Nile tilapia feeds enhanced the growth and feed performances, intestinal histomorphological features, growth-related genes, and immune response.

This study aimed to detect the virulent Salmonella serovars (including ESBLs producing) isolated from broiler chickens and humans. Three hundred broilers and sixty human fecal samples were bacteriologically examined. Thirty (10%) and fourteen (23.4%) Salmonella isolates were recovered from broiler and human samples, respectively. The most predominant serovar was S. enteritidis and S. typhimurium. All Salmonella isolates were confirmed by conventional PCR-based invA and ompA genes. Multidrug resistant (MDR) isolates were screened for the detection of adrA and csgD biofilm-associated genes, which were found in all isolated serovars except one S. typhimurium and 2 S. infantis of chicken isolates that were devoid of the adrA gene. Moreover, MDR isolates were screened for detection of seven resistance genes including ESBLs and other classes of resistance genes. Chicken isolates harbored blaTEM, int1, blaCTX and qnrS genes as 100, 27.8, 11.1 and 11.1%, respectively, while all human isolates harbored blaTEM, int1 and int3 genes. The genetic correlations between virulent Salmonella serovars (including antimicrobial resistance) avian and human origins were compared. In conclusion, the high prevalence of virulent ESBL producing Salmonella serovars in broilers and humans with genetic correlations between them might be zoonotic and public health hazards.

It is crucial to ensure the suitable stocking density for Nile tilapia fry since Nile tilapia is among the most consumed fish species globally. In this study, fry were distributed at three stocking densities, 1000 fry/m (low density, LD), 2000 fry/m3 (middle density, MD), and 4000 fry/m (high density, HD). Then each stocking density was subdivided into two groups where three aquaria were supplied with fresh dechlorinated water (FW, 0.35 g saline/L), and the other three aquaria were fortified with underground brackish water (BW, ≈ 8 g saline/L). Subsequently, fry were kept under these experimental conditions and offered 40% crude protein thrice daily at 4–5% of the body mass for 15 days, then switched to 30% thrice daily at 3–4% of the body mass until the end of the trial (60 days). Except for the dissolved oxygen, salinity, and total ammonia nitrogen, the measured water characteristics (temperature and pH) were not markedly (P˃0.05) affected by the stocking density or water salinity. Stocking density was a significant factor in the case of final body weight, weight gain, specific growth rate, and survival rate (P<0.05). The water salinity and stocking density markedly affected the digestive enzyme activity (protease, lipase, and amylase) (P<0.05). The stocking density, water salinity, and their interaction were significant factors in the lysozyme activity. The stocking density significantly affected the superoxide dismutase (SOD), catalase activity (CAT), and glutathione peroxidase (GPx), and fish fry in LD showed higher SOD, CAT, and GPx than fish in MD or HD in FW or BW (P<0.05). Malondialde-hyde (MDA) was affected by the stocking density, and fish fry in LD showed lower MDA than in MD or HD under FW or BW (P<0.05). Increased density to HD in the FW or BW induced slight degeneration of the intestinal mucosal lining. In conclusion, Nile tilapia fry can grow in brackish water (≈ 8 g saline/L) with 1000 to 2000 fry/m without affecting growth performance, feed utilization, digestive enzymes, intestinal histological features, and immune and antioxidative responses.

A comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (n = 25). Groups 1–4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota, respectively, as two doses. All Gs, except for 4 and 8, were intramuscularly challenged with vNDV-VII.1.1 at 28 days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided higher protection against vNDV-VII.1.1 challenge than LaSota. PAMP-H9N2 with live NDV vaccines induced more protection than the live vaccine alone.

The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of recovered from bovine endometritis samples. A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. A total of 58(48.3%) isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole- trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored , 1, A, S, bla , and H with a percentage of 100%, C (40%), and A (10%). H was the most prevalent biofilm-associated gene. The present study highlights the high prevalence of multi-drug- resistant associated with bovine endometritis. The detection of the H gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic .

Nigeria's Vision 2020 has expressed a bold desire for the country to be among the world's top 20 economies by the year 2020. The economy has posted impressive growth figures since 2003, driven by higher oil revenues and a series of home-grown economic reforms. The country is now firmly on the road to middle-income status. But what else do government and the private sector need to do to create the jobs and growth that will underpin the national development strategy? What are the challenges that Nigeria's businesses face today? 'An Assessment of the Investment Climate in Nigeria' provides answers to these questions. Based on a survey of 2,300 companies, it provides evidence-based recommendations designed to support Vision 2020 and the president's seven-point agenda. The authors find that government must move quickly to create jobs and reduce poverty. Key challenges include a desperate shortage of energy and a poor transportation network, as well as low levels of education and continuing unrest in the Niger delta. In addition, Nigeria's workers need to become more productive in order to compete in a globalized economy. As a matter of fact, they are less productive than workers in more dynamic countries, such as Brazil, China, and Kenya. Improving productivity will require simultaneous efforts to foster competition, improve specific aspects of the business environment, and facilitate better management and training within individual firms. In addition to the issues of productivity, Nigeria's best firms have not been able to expand their market share. Consequently, policy makers need to address and elimate obstacles to competition, including barriers to entry, convoluted taxation, property registration, and licensing.

A comparison of the efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was performed following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (n = 25). Groups 1–4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota, respectively, as two doses. All Gs, except for 4 and 8, were intramuscularly challenged with vNDV-VII.1.1 at 28 days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided higher protection against vNDV-VII.1.1 challenge than LaSota. PAMP-H9N2 with live NDV vaccines induced more protection than the live vaccine alone.

This study aimed to detect the virulent Salmonella serovars (including ESBLs producing) isolated from broiler chickens and humans. Three hundred broilers and sixty human fecal samples were bacteriologically examined. Thirty (10%) and fourteen (23.4%) Salmonella isolates were recovered from broiler and human samples, respectively. The most predominant serovar was S. enteritidis and S. typhimurium. All Salmonella isolates were confirmed by conventional PCR-based invA and ompA genes. Multidrug resistant (MDR) isolates were screened for the detection of adrA and csgD biofilm-associated genes, which were found in all isolated serovars except one S. typhimurium and 2 S. infantis of chicken isolates that were devoid of the adrA gene. Moreover, MDR isolates were screened for detection of seven resistance genes including ESBLs and other classes of resistance genes. Chicken isolates harbored bla[sub.TEM] , int1, bla[sub.CTX] and qnrS genes as 100, 27.8, 11.1 and 11.1%, respectively, while all human isolates harbored bla[sub.TEM] , int1 and int3 genes. The genetic correlations between virulent Salmonella serovars (including antimicrobial resistance) avian and human origins were compared. In conclusion, the high prevalence of virulent ESBL producing Salmonella serovars in broilers and humans with genetic correlations between them might be zoonotic and public health hazards.

Nigeria's vision of 2020 is a bold desire to be among the top twenty economies by the year 2020. The economy has posted impressive growth figures since 2003 driven by higher oil prices and a series of home-grown, economic reforms. The country is now firmly on the road to middle-income status. This Investment Climate Analysis is built on a 2,300 firm survey and provides evidence-based recommendations designed to support the vision 2020. Survey results represent investment climate status in Nigeria and are grouped by the following topics: firm productivity and business environment, comparison of state level investment climates, access to finance, entrepreneurship and managerial capacity in firms, and investment climate aspects. The authors conclude that stakeholder consultations on the diagnostic work, policy assessment, and design would improve Nigeria's investment climate.