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The silk gland of the domesticated silkworm Bombyx mori , is a remarkable organ that produces vast amounts of silk with exceptional properties. Little is known about which silk gland cells execute silk protein synthesis and its precise spatiotemporal control. Here, we use single-cell RNA sequencing to build a comprehensive cell atlas of the silkworm silk gland, consisting of 14,972 high-quality cells representing 10 distinct cell types, in three early developmental stages. We annotate all 10 cell types and determine their distributions in each region of the silk gland. Additionally, we decode the developmental trajectory and gene expression status of silk gland cells. Finally, we discover marker genes involved in the regulation of silk gland development and silk protein synthesis. Altogether, this work reveals the heterogeneity of silkworm silk gland cells and their gene expression dynamics, affording a deeper understanding of silk-producing organs at the single-cell level. The molecular underpinning of silk-producing organs is not well characterized. Here the authors use single-cell RNA sequencing to build an atlas of the silkworm silk gland and reveal the heterogeneity of silk gland cells.

Aquaculture has witnessed excellent growth in recent decades, with significant potential for protein nutrition security and jobs. Genomics has significantly enhanced aquaculture technologies across various species and culture processes, improving production efficiency and profitability. With the continuous advances in next-generation sequencing technologies and bioinformatics, substantial genomic resources have been obtained for dozens of aquaculture species. These include the development of high-quality whole-genome sequences, transcriptome databases, and molecular markers, mainly single nucleotide polymorphisms (SNPs), followed by the discovery of high-density linkages and physical maps. This review presents an overview of recent advancements in aquaculture genomics, including genome sequencing and assembly, molecular breeding, genetic characterization of traits, health and biodiversity assessments, and conservation biology of fish and shellfish. The review further examines the challenges impeding the effective implementation of genomics in aquaculture research, as the full potential remains underexploited within this sector. Finally, we highlight the available opportunities, including discussing the future priorities for technologies, auspicious science, and governmental policies for realizing the fullest benefit of genomic advancement in improving the aquaculture industry. The suggestions proposed in this review will facilitate using genomic-informed data to manage valuable aquaculture resources.

Recent advances in large language models (LLMs) have enabled social simulation through multi-agent systems. Prior efforts focus on agent societies created from scratch, assigning agents with newly defined personas. However, simulating established fictional worlds and characters remain largely underexplored, despite its significant practical value. In this paper, we introduce BookWorld, a comprehensive system for constructing and simulating book-based multi-agent societies. BookWorld's design covers comprehensive real-world intricacies, including diverse and dynamic characters, fictional worldviews, geographical constraints and changes, e.t.c. BookWorld enables diverse applications including story generation, interactive games and social simulation, offering novel ways to extend and explore beloved fictional works. Through extensive experiments, we demonstrate that BookWorld generates creative, high-quality stories while maintaining fidelity to the source books, surpassing previous methods with a win rate of 75.36%. The code of this paper can be found at the project page: https://bookworld2025.github.io/.

Role-playing agents (RPA) have been a popular application area for large language models (LLMs), attracting significant interest from both industry and academia.While existing RPAs well portray the characters' knowledge and tones, they face challenges in capturing their minds, especially for small role-playing language models (RPLMs). In this paper, we propose to enhance RPLMs via personality-indicative data. Specifically, we leverage questions from psychological scales and distill advanced RPAs to generate dialogues that grasp the minds of characters. Experimental results validate that RPLMs trained with our dataset exhibit advanced role-playing capabilities for both general and personality-related evaluations. Code and data are available at https://github.com/alienet1109/RolePersonalitythis URL.

Limited by the size of microelectronics, as well as the space of electrical vehicles, there are tremendous demands for lithium-ion batteries with high volumetric energy densities. Current lithium-ion batteries, however, adopt graphite-based anodes with low tap density and gravimetric capacity, resulting in poor volumetric performance metric. Here, by encapsulating nanoparticles of metallic tin in mechanically robust graphene tubes, we show tin anodes with high volumetric and gravimetric capacities, high rate performance, and long cycling life. Pairing with a commercial cathode material LiNi 0.6 Mn 0.2 Co 0.2 O 2 , full cells exhibit a gravimetric and volumetric energy density of 590 W h Kg −1 and 1,252 W h L −1 , respectively, the latter of which doubles that of the cell based on graphite anodes. This work provides an effective route towards lithium-ion batteries with high energy density for a broad range of applications. Here the authors report a tin anode design by encapsulating tin nanoparticles in graphene tubes. The design exhibits high capacity, good rate performance and cycling stability. Pairing with NMC, the full cell delivers a volumetric energy density twice as high as that for the commercial cell.

Background: In the era of longevity, many low- and middle-income countries (LMICs) still lack a comprehensive understanding of health deficits among older adults and the care burden associated with “unhealthy longevity”. This study aims to reveal future changes in care needs and pressure in China from 2030 to 2100. Method: This study develops a multistate demographic forecasting framework by integrating a Markov-based health state transition model with the conceptual logic of an age-shift algorithm. Transition probability matrices by age and gender are estimated using nationally representative microdata from the Chinese Longitudinal Healthy Longevity Survey (CLHLS). Baseline population data from the National Bureau of Statistics and WPP 2024 are then used to simulate the evolution of health status among older adults in China from 2030 to 2100. Finally, person-years with disability (PYD) are calculated to evaluate the projected magnitude, structure, and gender disparities of long-term care needs over time. Results: Between 2030 and 2100, the number of disabled older adults in China is projected to follow an inverted U-shaped trend—peaking at 160 million in 2070 and remaining above 115 million by 2100. The share of disabled individuals among older adults rises steadily, from 39.75% to 45.28%. Person-years with disability (PYD) show sustained growth, especially among the oldest-old and women. By 2100, adults aged 95 and older contribute over 20 million PYD—eight times the 2030 level. Gender disparities widen: in 2100, women aged 85–94 account for 53.94 million severe-disability PYD, exceeding men by 8.22 million. These trends reflect mounting structural pressures on China’s long-term care system, increasingly driven by age- and gender-specific disability burdens. Conclusions: If the current disability trend continues unchecked, health risks for older adults will grow over time. In the near future, China will face an extremely heavy care burden and pressure, which will severely impact its economic and social systems. Seizing this critical window for policy action and system improvement is crucial to reducing risks in the longevity era.

Purpose Aerobic exercise has shown beneficial effects in the prevention and treatment of non-alcoholic fatty liver disease (NAFLD). Nevertheless, the regulatory mechanism is not turely clear. Therefore, we aim to clarify the possible mechanism by investigating the effects of aerobic exercise on NAFLD and its mitochondrial dysfunction. Methods NAFLD rat model was established by feeding high fat diet. and used oleic acid (OA) to treat HepG2 cells. Changes in histopathology, lipid accumulation, apoptosis, body weight, and biochemical parameters were assessed. In addition, antioxidants, mitochondrial biogenesis and mitochondrial fusion and division were assessed. Results The obtained in vivo results showed that aerobic exercise significantly improved lipid accumulation and mitochondrial dysfunction induced by HFD, activated the level of Sirtuins1 (Srit1), and weakened the acetylation and activity of dynamic-related protein 1 (Drp1). In vitro results showed that activation of Srit1 inhibited OA-induced apoptosis in HepG2 cells and alleviated OA-induced mitochondrial dysfunction by inhibiting Drp1 acetylation and reducing Drp1 expression. Conclusion Aerobic exercise alleviates NAFLD and its mitochondrial dysfunction by activating Srit1 to regulate Drp1 acetylation. Our study clarifies the mechanism of aerobic exercise in alleviating NAFLD and its mitochondrial dysfunction and provides a new method for adjuvant treatment of NAFLD.

Acute graft-versus-host disease (GVHD) remains a major obstacle for the wider usage of allogeneic hematopoietic stem cell transplantation (allo-HSCT), which is an effective therapy for hematopoietic malignancy. Here we show that caspase-11, the cytosolic receptor for bacterial endotoxin (lipopolysaccharide: LPS), enhances GVHD severity. Allo-HSCT markedly increases the LPS-caspase-11 interaction, leading to the cleavage of gasdermin D (GSDMD). Caspase-11 and GSDMD mediate the release of interleukin-1α (IL-1α) in allo-HSCT. Deletion of Caspase-11 or Gsdmd , inhibition of LPS-caspase-11 interaction, or neutralizing IL-1α uniformly reduces intestinal inflammation, tissue damage, donor T cell expansion and mortality in allo-HSCT. Importantly, Caspase-11 deficiency does not decrease the graft-versus-leukemia (GVL) activity, which is essential to prevent cancer relapse. These findings have major implications for allo-HSCT, as pharmacological interference with the caspase-11 signaling might reduce GVHD while preserving GVL activity. An increasing number of inflammatory pathologies is associated with IL-1 production downstream of caspases 1 and 11. Here the authors show that graft-versus-host-disease (GvHD) is diminished in mice with genetic or pharmacological ablation of caspase-11, and provide mechanistic insights into the signals leading to caspase-11 activation in GvHD.

Aging increases the risk of a myriad of chronic diseases, which are expensive and difficult to treat owing to their various risk factors. Repurposing existing medications has accelerated the development of therapies aimed at slowing aging. In this study, using IMR90 cells and aged mice, we revealed that enalapril, a drug widely prescribed for hypertension, can improve both cellular senescence and individual health. Mechanistically, phosphorylated Smad1/5/9 act as pivotal mediators of the anti-senescence properties of enalapril. It stimulates downstream genes involved in cell cycle regulation and antioxidative defenses, facilitating cell proliferation and diminishing the production of reactive oxygen species (ROS), thus increasing the antioxidative ability of enalapril. At the organismal level, enalapril has been shown to bolster the physiological performance of various organs; it notably enhances memory capacity and renal function and relieves lipid accumulation. Our work highlights the potential of enalapril to augment antioxidative defenses and combat the effects of aging, thereby indicating its promise as a treatment strategy for aging-associated diseases and its use for healthy aging.

Background Evidence on the dose-response connection involving depressive symptoms and various types of physical activity in urban and rural populations is scarce. This study compares the dose-response connection across depressive symptoms and different types of activity in populations living in rural and urban areas. Methods The study is based on a cross-sectional weighted sample from the 2020 China Health and Retirement Longitudinal Study (CHARLS). Physical activity and depressive symptoms were assessed using self-reported activity from the CHARLS questionnaire and the Centre for Epidemiological Studies Depression Scale. Physical activity was computed in metabolic equivalents (METs) and depressive symptom cutoff is 10. 1 MET is the amount of oxygen used during rest (3.5 mL O2/kg/min). Multivariate logistic regression analyses were used to determine the dose-response relationship between different types of physical activity and depressive symptoms. Results Physical activity of different types showed a varying relationship with depressive symptoms with dose in rural populations. However, in urban populations, recreational physical activity exhibited a negative correlation with depressive symptoms, while non-recreational activity displayed a positive correlation. Specifically, in the rural population, 600-3,000 METs-min/week of recreational physical activity was inversely linked to depressive symptoms, but recreational physical activity exceeding 3,000 METs-min/week was associated with a 13.2% risk increase in depressive symptoms (OR = 1.132, 95% CI: 1.131–1.132), and over 6,000 METs-min/week of non-recreational activity was similarly associated with an increased risk of depressive symptoms. In the urban population, recreational physical activity was related to a reduced incidence of depressive symptoms, with a maximum reduction of 71.7% at doses up to 9,000–12,000 METs min/week (OR = 0.283, 95% CI:0.282–0.284), and 6,000–9,000 non-recreational physical activities, the odds of increased risk of depressive symptoms were highest (OR = 2.224, 95% CI:2.221–2.227). Conclusions Our study found a non-linear dose correlation between physical activities and depressive symptoms. The dose-response connection between different physical activity types and depressive symptoms varies between rural and urban areas. Recreational physical activity is recommended for urban and reasonable amounts of nonrecreational physical activity for rural populations to reduce the risk of depressive symptoms. However, causal interpretations cannot be made due to the cross-sectional design of the study.