Explore the vast range of titles available.

Previous studies support the textbook model that shape and color are extracted by distinct neurons in primate primary visual cortex (V1). However, rigorous testing of this model requires sampling a larger stimulus space than previously possible. We used stable GCaMP6f expression and two-photon calcium imaging to probe a very large spatial and chromatic visual stimulus space and map functional microarchitecture of thousands of neurons with single-cell resolution. Notable proportions of V1 neurons strongly preferred equiluminant color over achromatic stimuli and were also orientation selective, indicating that orientation and color in V1 are mutually processed by overlapping circuits. Single neurons could precisely and unambiguously code for both color and orientation. Further analyses revealed systematic spatial relationships between color tuning, orientation selectivity, and cytochrome oxidase histology.

Studies of color perception have led to mechanistic models of how cone-opponent signals from retinal ganglion cells are integrated to generate color appearance. But it is unknown how this hypothesized integration occurs in the brain. Here we show that cone-opponent signals transmitted from retina to primary visual cortex (V1) are integrated through highly organized circuits within V1 to implement the color opponent interactions required for color appearance. Combining intrinsic signal optical imaging (ISI) and 2-photon calcium imaging (2PCI) at single cell resolution, we demonstrate cone-opponent functional domains (COFDs) that combine L/M cone-opponent and S/L + M cone-opponent signals following the rules predicted from psychophysical studies of color perception. These give rise to an orderly organization of hue preferences of the neurons within the COFDs and the generation of hue “pinwheels”. Thus, spatially organized neural circuits mediate an orderly transition from cone-opponency to color appearance that begins in V1. Psychophysical studies predict that retinal cone-opponent mechanisms interact in specific combinations to generate color perception. Here, the authors show that the predicted interactions are implemented by organized circuits in primary visual cortex.

Relative permeability (kr) and the capillary pressure (Pc) are the central key elements defining the multiphase fluids flow behavior in the porous media. However, the dynamic capillarity should consider the dynamic relative permeability and the dynamic capillary pressure while performing waterflooding process in extremely low permeable formations. In order to improve the oil production, the advanced horizontal well drilling along with multiple hydraulic fracturing is generally instigated to penetrate the unconventional resources. The aim of this study is to consider the dynamic capillarity in a commercial reservoir simulation, while utilizing the data gained from the dynamic and steady experiments of the relative permeability and the capillary pressure impacts during waterflooding process in the core plugs of unconventional tight oil reservoirs. The commercial reservoir simulation conducted sensitivity analyses using Computer Modeling Group simulator. The outcomes show that the well production of the reservoir is overestimated while implementing steady data for forecasting due to which the oil saturation decreases more equally and further rapidly. Additionally, the forecast of the well production estimated to breakthrough sooner. However, neglecting the dynamic capillarity causes a huge breakthrough of water influx. Therefore, the core objective of this study is to probe the consequences of taking into consideration the dynamic capillarity in ultra-low permeable formations while giving an alternative perspective to forecast the production of the hydraulically fractured unconventional tight oil reservoirs.

The ability to target and manipulate specific neuronal populations is crucial for understanding brain function. In this report, the authors describe a novel virus that restricts gene expression to telencephalic GABAergic interneurons, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in mice and in non-genetically tractable species. A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.

Nat. Neurosci. 19, 1743–1749 (2016); published online 31 October 2016; corrected after print 29 November 2016 In the version of this article initially published, authors Joshua S. Grimley, Anne-Rachel Krostag and Ajamete Kaykas were missing. These authors have been inserted into the author list after Jianhua Chu; they are at the Allen Institute for Brain Science, Seattle, Washington, USA, and performed experiments related to hESCs.

Studies of color perception have led to mechanistic models of how cone-opponent signals from retinal ganglion cells are integrated to generate color appearance. But it is unknown how this hypothesized integration occurs in the brain. Here we show that cone-opponent signals transmitted from retina to primary visual cortex (V1) are integrated through highly organized circuits within V1 to implement the color opponent interactions required for color appearance. Combining intrinsic signal optical imaging (ISI) and 2-photon calcium imaging (2PCI) at single cell resolution, we demonstrate cone-opponent functional domains (COFDs) that combine L/M cone-opponent and S/L+M cone-opponent signals following the rules predicted from psychophysical studies of color perception. These give rise to an orderly organization of hue preferences of the neurons within the COFDs and the generation of hue “pinwheels”. Thus, spatially organized neural circuits mediate an orderly transition from cone-opponency to color appearance that begins in V1.

Abstract Studies of color perception have led to mechanistic models of how signals from cone-opponent retinal ganglion cells are integrated to generate color appearance. But it is not known where or how these hypothesized mechanisms occur in the brain. Here we show that cone opponent signals transmitted from the retina to primary visual cortex (V1) are integrated through highly organized circuits within V1 to generate the color opponent mechanisms that underlie color appearance. Combining intrinsic signal optical imaging (ISI) and 2-photon calcium imaging (2PCI) at single cell resolution, we demonstrate cone-opponent functional domains (COFDs) that combine L/M cone-opponent and S/L+M cone-opponent signals in precisely the combinations predicted from psychophysical studies of color perception. These give rise to an orderly organization of hue preferences of the neurons within the COFDs and the generation of hue “pinwheels”. COFDs occupy regions corresponding to both high and low cytochrome oxidase intensity (“blobs” and “interblobs”) but have a bias toward blobs. Thus, neural circuits in the primary visual cortex establish the boundary conditions for color opponency and unique hues. One Sentence Summary Cone-opponent functional domains generate color opponent functional architecture in primary visual cortex. Competing Interest Statement The authors have declared no competing interest.